Rh5CG495300

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004026
Physical Location & Seq
Forward (+)
68862773 .. 68863851
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG495300.1

Sequence Viewer

Length: 372 bp
ATGGACTGCGAAGGTTGCGAGAGAAGAGTGAAGAAATCAGTGGAAGGCATGAAAGGAGTCACTAGTGTTGAAGTGGATCCCAAGCAAAGCAAACTCACTGTTATTGGCTATGTGGACCCCAACAAGGTGTTGCATCGTGTCAGGCATCGAACGGGGAAGAAGGCGGATCTGTGGCCGTATGTACCATACGACGTCGTCGAGCATCCTTGGGCTCCCGGGGCTTATGATAGGAAGGCACCACCTGGGTACGTGAGGAACGTGCTGAATGACCCTGACCCTCAGGCTTCTGCTCTCGCACGTGCCAACTCCACCGAGGTGAAGTATACGACGGCGTTTAGTGACGAAAATCCAAATGCTTGTGTGGTTATGTAG

Protein Analysis

123

Amino Acids

13.71

Weight (kDa)

8.43

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 1 - 53 2e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 195
AccB1I GGYRCC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 323
AciI CCGC 1 cut(s) 164
AclWI GGATC 3 cut(s) 71, 84, 174
AcoI YGGCCR 1 cut(s) 173
AcvI CACGTG 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 183, 248
AfiI CCNNNNNNNGG 1 cut(s) 124
AgsI TTSAA 1 cut(s) 71
AhlI ACTAGT 1 cut(s) 62
AjnI CCWGG 1 cut(s) 241
AleI CACNNNNGTG 1 cut(s) 314
AlwI GGATC 3 cut(s) 71, 84, 174
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 1 cut(s) 173
AspS9I GGNCC 1 cut(s) 115
AsuC2I CCSGG 2 cut(s) 216, 217
AsuHPI GGTGA 1 cut(s) 328
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 1 cut(s) 115
AxyI CCTNAGG 1 cut(s) 279
BamHI GGATCC 1 cut(s) 76
BanI GGYRCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 214
BbrPI CACGTG 1 cut(s) 299
BceAI ACGGC 2 cut(s) 160, 345
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 2 cut(s) 216, 217
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 1 cut(s) 63
Bme1390I CCNGG 3 cut(s) 216, 217, 243
Bme18I GGWCC 1 cut(s) 115
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 4 cut(s) 78, 117, 213, 237
BmrFI CCNGG 3 cut(s) 216, 217, 243
BmsI GCATC 3 cut(s) 142, 154, 211
BpuMI CCSGG 2 cut(s) 216, 217
BsaAI YACGTR 2 cut(s) 250, 299
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 5 cut(s) 206, 215, 216, 242, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse21I CCTNAGG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 5 cut(s) 206, 215, 216, 242, 312
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 293
BshFI GGCC 1 cut(s) 175
BshNI GGYRCC 1 cut(s) 235
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 1 cut(s) 216
BslI CCNNNNNNNGG 1 cut(s) 124
BsnI GGCC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 2 cut(s) 76, 166
BspACI CCGC 1 cut(s) 164
BspANI GGCC 1 cut(s) 175
BspCNI CTCAG 1 cut(s) 292
BspLI GGNNCC 4 cut(s) 78, 117, 213, 237
BspPI GGATC 3 cut(s) 71, 84, 174
BspT107I GGYRCC 1 cut(s) 235
BssECI CCNNGG 5 cut(s) 206, 215, 216, 242, 312
BssMI GATC 2 cut(s) 76, 166
BssNAI GTATAC 1 cut(s) 324
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 206
Bst1107I GTATAC 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 192
BstBAI YACGTR 2 cut(s) 250, 299
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 202
BstKTI GATC 2 cut(s) 79, 169
BstMBI GATC 2 cut(s) 76, 166
BstMWI GCNNNNNNNGC 2 cut(s) 15, 218
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 3 cut(s) 214, 215, 241
BstX2I RGATCY 2 cut(s) 76, 166
BstYI RGATCY 2 cut(s) 76, 166
BstZ17I GTATAC 1 cut(s) 324
Bsu36I CCTNAGG 1 cut(s) 279
BsuRI GGCC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 45, 96
Cfr13I GGNCC 1 cut(s) 115
Cfr9I CCCGGG 1 cut(s) 215
Csp6I GTAC 2 cut(s) 182, 247
CviAII CATG 1 cut(s) 49
CviJI RGCY 5 cut(s) 108, 175, 212, 221, 284
CviKI_1 RGCY 5 cut(s) 108, 175, 212, 221, 284
CviQI GTAC 2 cut(s) 182, 247
DdeI CTNAG 1 cut(s) 279
DpnI GATC 2 cut(s) 78, 168
DpnII GATC 2 cut(s) 76, 166
EaeI YGGCCR 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
EciI GGCGGA 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 206
Eco24I GRGCYC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 115
Eco72I CACGTG 1 cut(s) 299
Eco81I CCTNAGG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 206
EcoT38I GRGCYC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 1 cut(s) 52
FaiI YATR 7 cut(s) 50, 111, 180, 187, 225, 324, 368
FatI CATG 1 cut(s) 48
FblI GTMKAC 1 cut(s) 323
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 214
FspBI CTAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 175
HapII CCGG 1 cut(s) 216
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 1 cut(s) 52
HinfI GANTC 1 cut(s) 57
HpaII CCGG 1 cut(s) 216
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 115, 324
Hpy8I GTNNAC 2 cut(s) 115, 324
Hpy99I CGWCG 4 cut(s) 194, 197, 200, 331
HpyAV CCTTC 4 cut(s) 5, 38, 154, 226
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4IV ACGT 4 cut(s) 192, 249, 258, 298
HpyCH4V TGCA 1 cut(s) 133
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 218
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 4 cut(s) 192, 249, 258, 298
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 1 cut(s) 52
Kzo9I GATC 2 cut(s) 76, 166
LmnI GCTCC 1 cut(s) 217
LpnPI CCDG 6 cut(s) 127, 228, 229, 255, 266, 285
LweI GCATC 3 cut(s) 142, 154, 211
MaeI CTAG 1 cut(s) 63
MaeII ACGT 4 cut(s) 192, 249, 258, 298
MaeIII GTNAC 2 cut(s) 58, 338
MalI GATC 2 cut(s) 78, 168
MboI GATC 2 cut(s) 76, 166
MboII GAAGA 3 cut(s) 36, 43, 169
MflI RGATCY 2 cut(s) 76, 166
MhlI GDGCHC 1 cut(s) 214
MlyI GAGTC 1 cut(s) 66
MnlI CCTC 3 cut(s) 246, 288, 307
MslI CAYNNNNRTG 1 cut(s) 314
MspI CCGG 1 cut(s) 216
MspR9I CCNGG 3 cut(s) 216, 217, 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 2 cut(s) 15, 218
NciI CCSGG 2 cut(s) 216, 217
NdeII GATC 2 cut(s) 76, 166
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 4 cut(s) 78, 117, 213, 237
NmuCI GTSAC 2 cut(s) 58, 338
OliI CACNNNNGTG 1 cut(s) 314
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 255
PflFI GACNNNGTC 1 cut(s) 194
PleI GAGTC 1 cut(s) 65
PmaCI CACGTG 1 cut(s) 299
PmlI CACGTG 1 cut(s) 299
PpsI GAGTC 1 cut(s) 65
Ppu21I YACGTR 2 cut(s) 250, 299
Psp6I CCWGG 1 cut(s) 241
PspCI CACGTG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 4 cut(s) 78, 117, 213, 237
PspPI GGNCC 1 cut(s) 115
PsuI RGATCY 2 cut(s) 76, 166
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 2 cut(s) 183, 248
RsaNI GTAC 2 cut(s) 182, 247
RseI CAYNNNNRTG 1 cut(s) 314
Sau3AI GATC 2 cut(s) 76, 166
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 3 cut(s) 216, 217, 243
SduI GDGCHC 1 cut(s) 214
SetI ASST 8 cut(s) 16, 129, 195, 244, 252, 261, 301, 318
SfaNI GCATC 3 cut(s) 142, 154, 211
SinI GGWCC 1 cut(s) 115
SmaI CCCGGG 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 314
SpeI ACTAGT 1 cut(s) 62
SsiI CCGC 1 cut(s) 164
SspMI CTAG 1 cut(s) 63
StyD4I CCNGG 3 cut(s) 214, 215, 241
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 1 cut(s) 100
TaiI ACGT 4 cut(s) 195, 252, 261, 301
TaqI TCGA 2 cut(s) 148, 198
TscAI CASTG 2 cut(s) 45, 103
TseFI GTSAC 2 cut(s) 58, 338
Tsp45I GTSAC 2 cut(s) 58, 338
TspDTI ATGAA 1 cut(s) 65
TspMI CCCGGG 1 cut(s) 215
TspRI CASTG 2 cut(s) 45, 103
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 115
XmaI CCCGGG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 323
XspI CTAG 1 cut(s) 63
ZraI GACGTC 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.