Rw4G011560

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004026
Physical Location & Seq
Forward (+)
27346395 .. 27346972
578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011560.1

Sequence Viewer

Length: 438 bp
ATGGTCTATATCTTGCAGCCAAATCATGCTTTTTTTGGGAGTATCTCAAGCTTGCCAGTGGGTTTTCTCAGTAAAGTCTCCGAGCTCCGTGCTAGGGCTCGGAGTCATAAAAAGCTCAAGAAGAACAAGCAGCTACAGACTGTGGAGATAAAAGTGAAGATGGACTGCGAAGGTTGCGAGAGAAGAGTGAAGAAATCAGTGGAAGGCATGAAAGGAGTCACTAGTGTTGAAGTGGATCCCAAGCAAAGCAAGCTCACTGTTATTGGCAATGTGGACCCCAACAAGGTGTTGCATCGTGTCAGGCATCGAACGGGGAAGAAGCCGGATCTGTGGCCGTATGTACCATACGACGTCGTCGAGCATCCATGGGCTCCCGGGGCTTATGATAGGAAGGCACCACCTGGGTACGTGAGGAACTTGCTAAATGACCCTGACTAG

Protein Analysis

145

Amino Acids

16.47

Weight (kDa)

9.85

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 51 - 106 2.5e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
AccB1I GGYRCC 1 cut(s) 394
AclWI GGATC 3 cut(s) 230, 243, 333
AcoI YGGCCR 1 cut(s) 332
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 2 cut(s) 342, 407
AfiI CCNNNNNNNGG 2 cut(s) 94, 283
AgsI TTSAA 1 cut(s) 230
AhlI ACTAGT 1 cut(s) 221
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 5 cut(s) 51, 85, 115, 133, 253
AluI AGCT 5 cut(s) 51, 85, 115, 133, 253
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 3 cut(s) 230, 243, 333
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 1 cut(s) 332
ApeKI GCWGC 2 cut(s) 16, 130
AspS9I GGNCC 1 cut(s) 274
AsuC2I CCSGG 2 cut(s) 375, 376
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 274
BamHI GGATCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 3 cut(s) 87, 100, 373
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 28, 142
BccI CCATC 1 cut(s) 154
BceAI ACGGC 1 cut(s) 319
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 1 cut(s) 402
BcnI CCSGG 2 cut(s) 375, 376
BcoDI GTCTC 1 cut(s) 82
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 3 cut(s) 93, 222, 436
BfmI CTRYAG 1 cut(s) 134
BisI GCNGC 2 cut(s) 17, 131
BlsI GCNGC 2 cut(s) 18, 132
Bme1390I CCNGG 3 cut(s) 375, 376, 402
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 4 cut(s) 237, 276, 372, 396
BmrFI CCNGG 3 cut(s) 375, 376, 402
BmsI GCATC 3 cut(s) 301, 313, 370
BpuEI CTTGAG 2 cut(s) 31, 101
BpuMI CCSGG 2 cut(s) 375, 376
BsaAI YACGTR 1 cut(s) 409
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 365, 374, 375, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 283
Bse1I ACTGG 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 4 cut(s) 365, 374, 375, 401
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 2 cut(s) 94, 283
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 82
BseNI ACTGG 1 cut(s) 56
BseXI GCAGC 2 cut(s) 28, 142
BshFI GGCC 1 cut(s) 334
BshNI GGYRCC 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 2 cut(s) 323, 375
BslI CCNNNNNNNGG 2 cut(s) 94, 283
BsmAI GTCTC 1 cut(s) 82
BsnI GGCC 1 cut(s) 334
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 87, 100, 373
Bsp143I GATC 2 cut(s) 235, 325
Bsp19I CCATGG 1 cut(s) 365
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 1 cut(s) 81
BspLI GGNNCC 4 cut(s) 237, 276, 372, 396
BspPI GGATC 3 cut(s) 230, 243, 333
BspT107I GGYRCC 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 274
BsrI ACTGG 1 cut(s) 56
BssECI CCNNGG 4 cut(s) 365, 374, 375, 401
BssMI GATC 2 cut(s) 235, 325
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 2 cut(s) 142, 259
Bst6I CTCTTC 1 cut(s) 178
BstACI GRCGYC 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 2 cut(s) 53, 251
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 1 cut(s) 365
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 2 cut(s) 238, 328
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 2 cut(s) 235, 325
BstMWI GCNNNNNNNGC 3 cut(s) 174, 250, 377
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 3 cut(s) 373, 374, 400
BstSFI CTRYAG 1 cut(s) 134
BstV1I GCAGC 2 cut(s) 28, 142
BstX2I RGATCY 2 cut(s) 235, 325
BstYI RGATCY 2 cut(s) 235, 325
BsuRI GGCC 1 cut(s) 334
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 3 cut(s) 63, 204, 255
Cac8I GCNNGC 2 cut(s) 53, 251
Cfr13I GGNCC 1 cut(s) 274
Cfr9I CCCGGG 1 cut(s) 374
Csp6I GTAC 2 cut(s) 341, 406
CviAII CATG 3 cut(s) 26, 208, 366
CviQI GTAC 2 cut(s) 341, 406
DdeI CTNAG 1 cut(s) 68
DpnI GATC 2 cut(s) 237, 327
DpnII GATC 2 cut(s) 235, 325
EaeI YGGCCR 1 cut(s) 332
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 365
Eco24I GRGCYC 3 cut(s) 87, 100, 373
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 365
EcoT38I GRGCYC 3 cut(s) 87, 100, 373
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 3 cut(s) 29, 211, 369
FaiI YATR 8 cut(s) 9, 27, 108, 209, 339, 346, 367, 384
FatI CATG 3 cut(s) 25, 207, 365
Fnu4HI GCNGC 2 cut(s) 17, 131
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 3 cut(s) 87, 100, 373
Fsp4HI GCNGC 2 cut(s) 17, 131
FspBI CTAG 3 cut(s) 93, 222, 436
GluI GCNGC 2 cut(s) 17, 131
HaeIII GGCC 1 cut(s) 334
HapII CCGG 2 cut(s) 323, 375
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 3 cut(s) 29, 211, 369
HindIII AAGCTT 1 cut(s) 49
HinfI GANTC 2 cut(s) 103, 216
HpaII CCGG 2 cut(s) 323, 375
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 2 cut(s) 82, 102
Hpy188III TCNNGA 1 cut(s) 118
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 3 cut(s) 353, 356, 359
HpyAV CCTTC 3 cut(s) 164, 197, 385
HpyCH4III ACNGT 2 cut(s) 142, 259
HpyCH4IV ACGT 2 cut(s) 351, 408
HpyCH4V TGCA 2 cut(s) 16, 292
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 250, 377
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 2 cut(s) 351, 408
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 3 cut(s) 29, 211, 369
Kzo9I GATC 2 cut(s) 235, 325
LmnI GCTCC 2 cut(s) 90, 376
LpnPI CCDG 6 cut(s) 69, 286, 336, 387, 388, 414
Lsp1109I GCAGC 2 cut(s) 28, 142
LweI GCATC 3 cut(s) 301, 313, 370
MaeI CTAG 3 cut(s) 93, 222, 436
MaeII ACGT 2 cut(s) 351, 408
MaeIII GTNAC 1 cut(s) 217
MalI GATC 2 cut(s) 237, 327
MboI GATC 2 cut(s) 235, 325
MboII GAAGA 5 cut(s) 133, 169, 195, 202, 328
MflI RGATCY 2 cut(s) 235, 325
MhlI GDGCHC 3 cut(s) 87, 100, 373
MlyI GAGTC 2 cut(s) 112, 225
MnlI CCTC 1 cut(s) 405
MspI CCGG 2 cut(s) 323, 375
MspR9I CCNGG 3 cut(s) 375, 376, 402
MvaI CCWGG 1 cut(s) 402
MwoI GCNNNNNNNGC 3 cut(s) 174, 250, 377
NciI CCSGG 2 cut(s) 375, 376
NcoI CCATGG 1 cut(s) 365
NdeII GATC 2 cut(s) 235, 325
NlaIII CATG 3 cut(s) 29, 211, 369
NlaIV GGNNCC 4 cut(s) 237, 276, 372, 396
NmuCI GTSAC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PflFI GACNNNGTC 1 cut(s) 353
PkrI GCNGC 2 cut(s) 18, 132
PleI GAGTC 2 cut(s) 111, 224
PpsI GAGTC 2 cut(s) 111, 224
Ppu21I YACGTR 1 cut(s) 409
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 4 cut(s) 237, 276, 372, 396
PspPI GGNCC 1 cut(s) 274
PsuI RGATCY 2 cut(s) 235, 325
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 2 cut(s) 342, 407
RsaNI GTAC 2 cut(s) 341, 406
SacI GAGCTC 1 cut(s) 87
SatI GCNGC 2 cut(s) 17, 131
Sau3AI GATC 2 cut(s) 235, 325
Sau96I GGNCC 1 cut(s) 274
SchI GAGTC 2 cut(s) 112, 225
ScrFI CCNGG 3 cut(s) 375, 376, 402
SduI GDGCHC 3 cut(s) 87, 100, 373
SfaNI GCATC 3 cut(s) 301, 313, 370
SfcI CTRYAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 274
SmaI CCCGGG 1 cut(s) 376
SmlI CTYRAG 2 cut(s) 46, 116
SmoI CTYRAG 2 cut(s) 46, 116
SpeI ACTAGT 1 cut(s) 221
SspMI CTAG 3 cut(s) 93, 222, 436
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 3 cut(s) 373, 374, 400
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 2 cut(s) 142, 259
TaiI ACGT 2 cut(s) 354, 411
TaqI TCGA 2 cut(s) 307, 357
TscAI CASTG 3 cut(s) 63, 204, 262
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 2 cut(s) 16, 130
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 224
TspGWI ACGGA 1 cut(s) 77
TspMI CCCGGG 1 cut(s) 374
TspRI CASTG 3 cut(s) 63, 204, 262
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 274
XmaI CCCGGG 1 cut(s) 374
XspI CTAG 3 cut(s) 93, 222, 436
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.