pycom02g09890

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004026
Physical Location & Seq
Reverse (-)
6839955 .. 6841169
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09890.1

Sequence Viewer

Length: 582 bp
ATGCTGATGTGTATATATATGTTGCAACCTGCAAGGCAAACTTTAGGGTTTTATAACTTCGTAAGCAGCATTGTCCCACTTTTTCTAGTTTTATCTTTGCACGTGTGTGTGGCAATGGGTTTTCTCAGTAAAGTCTCTGAGCTTGGAGAGTGGCATCGTAGTAGTAAAAAGCTCAAAAAGAACAAGCAATTGAAGACGGTGGAGATTAAAGTGAAGATGGACTGTGAAGGGTGCGAGAGAAGAGTGAAGAAATCAGTGCAAGGCATGAAAGGAGTCACGGAGGTTGAGGTGGACCCCAAGCAGAGCAAGCTCACTGTGATTGGCTATGTGGACCCCGACAAAGTCTTGCATCGTGTTAGGCATCGAACGGGGAAGAAGGCTGAGCTCTGGCCTTACGTGCCGTACGATGTCGTTCCGCATCCCTACGCCCCGGAGGCTTATGACAAGAAGGCCCCACCTGGGTACGTGCGGAACGTGCTGGTGGATCCCGAGGCTTCGGCTCTCGCACGTGCAAGCTCCACTGAAGTGAAGTATACGACGGCGTTTAGTGACGAGAATCCAAATGCGTGTGTCGTCATGTAG

Protein Analysis

194

Amino Acids

21.78

Weight (kDa)

9.29

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 70 - 125 2.7e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 54
Acc36I ACCTGC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 533
AciI CCGC 2 cut(s) 416, 469
AclWI GGATC 2 cut(s) 479, 492
AcuI CTGAAG 1 cut(s) 543
AcvI CACGTG 2 cut(s) 103, 509
AfaI GTAC 2 cut(s) 404, 464
AfiI CCNNNNNNNGG 1 cut(s) 459
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 1 cut(s) 193
AjnI CCWGG 1 cut(s) 457
AleI CACNNNNGTG 2 cut(s) 105, 524
AluBI AGCT 5 cut(s) 142, 172, 310, 385, 516
AluI AGCT 5 cut(s) 142, 172, 310, 385, 516
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 2 cut(s) 479, 492
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 2 cut(s) 389, 450
ApeKI GCWGC 1 cut(s) 66
AspS9I GGNCC 3 cut(s) 292, 331, 451
AsuC2I CCSGG 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 2 cut(s) 292, 331
BamHI GGATCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 387
BbrPI CACGTG 2 cut(s) 103, 509
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 78
BccI CCATC 1 cut(s) 211
BceAI ACGGC 2 cut(s) 385, 555
BciT130I CCWGG 1 cut(s) 459
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 86
BfuAI ACCTGC 1 cut(s) 37
BglI GCCNNNNNGGC 1 cut(s) 434
BisI GCNGC 1 cut(s) 67
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 431, 459
Bme18I GGWCC 2 cut(s) 292, 331
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 3 cut(s) 292, 331, 451
BmiI GGNNCC 4 cut(s) 294, 333, 453, 486
BmrFI CCNGG 2 cut(s) 431, 459
BmsI GCATC 4 cut(s) 163, 358, 370, 427
BpiI GAAGAC 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 381
BpuMI CCSGG 1 cut(s) 431
BsaAI YACGTR 4 cut(s) 103, 397, 466, 509
BsaJI CCNNGG 3 cut(s) 429, 458, 489
Bsc4I CCNNNNNNNGG 1 cut(s) 459
Bse3DI GCAATG 1 cut(s) 120
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 429, 458, 489
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 1 cut(s) 459
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 3 cut(s) 129, 139, 372
BseXI GCAGC 1 cut(s) 78
BshFI GGCC 2 cut(s) 391, 452
BsiHKAI GWGCWC 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 1 cut(s) 431
BsiWI CGTACG 1 cut(s) 402
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 459
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 59
BsnI GGCC 2 cut(s) 391, 452
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 1 cut(s) 484
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 2 cut(s) 416, 469
BspANI GGCC 2 cut(s) 391, 452
BspCNI CTCAG 3 cut(s) 130, 138, 373
BspLI GGNNCC 4 cut(s) 294, 333, 453, 486
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 2 cut(s) 479, 492
BsrDI GCAATG 1 cut(s) 120
BssECI CCNNGG 3 cut(s) 429, 458, 489
BssMI GATC 1 cut(s) 484
BssNAI GTATAC 1 cut(s) 534
Bst1107I GTATAC 1 cut(s) 534
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 3 cut(s) 199, 224, 316
Bst6I CTCTTC 1 cut(s) 235
BstBAI YACGTR 4 cut(s) 103, 397, 466, 509
BstC8I GCNNGC 2 cut(s) 308, 514
BstDEI CTNAG 3 cut(s) 125, 138, 381
BstF5I GGATG 1 cut(s) 418
BstKTI GATC 1 cut(s) 487
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 1 cut(s) 484
BstMWI GCNNNNNNNGC 4 cut(s) 307, 397, 434, 475
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 2 cut(s) 429, 457
BstV1I GCAGC 1 cut(s) 78
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
BstZ17I GTATAC 1 cut(s) 534
BsuRI GGCC 2 cut(s) 391, 452
BtsCI GGATG 1 cut(s) 418
BtsIMutI CAGTG 3 cut(s) 261, 312, 519
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 2 cut(s) 308, 514
Cfr13I GGNCC 3 cut(s) 292, 331, 451
Csp6I GTAC 2 cut(s) 403, 463
CviAII CATG 2 cut(s) 265, 577
CviQI GTAC 2 cut(s) 403, 463
DdeI CTNAG 3 cut(s) 125, 138, 381
DpnI GATC 1 cut(s) 486
DpnII GATC 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 235
Ecl136II GAGCTC 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 292, 331
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 543
Eco72I CACGTG 2 cut(s) 103, 509
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 387
FaeI CATG 2 cut(s) 268, 580
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FaqI GGGAC 1 cut(s) 59
FatI CATG 2 cut(s) 264, 576
FblI GTMKAC 1 cut(s) 533
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 1 cut(s) 405
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 67
FspBI CTAG 1 cut(s) 86
GluI GCNGC 1 cut(s) 67
HaeIII GGCC 2 cut(s) 391, 452
HapII CCGG 1 cut(s) 431
Hin1II CATG 2 cut(s) 268, 580
HinfI GANTC 2 cut(s) 273, 556
HpaII CCGG 1 cut(s) 431
Hpy166II GTNNAC 3 cut(s) 292, 331, 534
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 488
Hpy8I GTNNAC 3 cut(s) 292, 331, 534
Hpy99I CGWCG 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 221, 370, 442
HpyCH4III ACNGT 3 cut(s) 199, 224, 316
HpyCH4IV ACGT 5 cut(s) 102, 396, 465, 474, 508
HpyCH4V TGCA 6 cut(s) 25, 32, 100, 259, 349, 512
HpyF10VI GCNNNNNNNGC 4 cut(s) 307, 397, 434, 475
HpyF3I CTNAG 3 cut(s) 125, 138, 381
HpySE526I ACGT 5 cut(s) 102, 396, 465, 474, 508
Hsp92II CATG 2 cut(s) 268, 580
Kzo9I GATC 1 cut(s) 484
LmnI GCTCC 1 cut(s) 521
LpnPI CCDG 6 cut(s) 42, 373, 444, 444, 464, 471
Lsp1109I GCAGC 1 cut(s) 78
LweI GCATC 4 cut(s) 163, 358, 370, 427
MaeI CTAG 1 cut(s) 86
MaeII ACGT 5 cut(s) 102, 396, 465, 474, 508
MaeIII GTNAC 2 cut(s) 274, 548
MalI GATC 1 cut(s) 486
MboI GATC 1 cut(s) 484
MboII GAAGA 5 cut(s) 205, 226, 252, 259, 385
MfeI CAATTG 1 cut(s) 188
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 1 cut(s) 188
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 4 cut(s) 274, 280, 427, 484
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 2 cut(s) 105, 524
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 2 cut(s) 431, 459
MunI CAATTG 1 cut(s) 188
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 4 cut(s) 307, 397, 434, 475
NciI CCSGG 1 cut(s) 431
NdeII GATC 1 cut(s) 484
NlaIII CATG 2 cut(s) 268, 580
NlaIV GGNNCC 4 cut(s) 294, 333, 453, 486
NmuCI GTSAC 2 cut(s) 274, 548
OliI CACNNNNGTG 2 cut(s) 105, 524
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 471
PfeI GAWTC 1 cut(s) 556
Pfl23II CGTACG 1 cut(s) 402
PflFI GACNNNGTC 1 cut(s) 341
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 1 cut(s) 281
PmaCI CACGTG 2 cut(s) 103, 509
PmlI CACGTG 2 cut(s) 103, 509
PpsI GAGTC 1 cut(s) 281
Ppu21I YACGTR 4 cut(s) 103, 397, 466, 509
PsiI TTATAA 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 457
PspCI CACGTG 2 cut(s) 103, 509
PspGI CCWGG 1 cut(s) 457
PspLI CGTACG 1 cut(s) 402
PspN4I GGNNCC 4 cut(s) 294, 333, 453, 486
PspPI GGNCC 3 cut(s) 292, 331, 451
PsuI RGATCY 1 cut(s) 484
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 2 cut(s) 404, 464
RsaNI GTAC 2 cut(s) 403, 463
RseI CAYNNNNRTG 2 cut(s) 105, 524
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 1 cut(s) 484
Sau96I GGNCC 3 cut(s) 292, 331, 451
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 2 cut(s) 431, 459
SduI GDGCHC 1 cut(s) 387
SfaNI GCATC 4 cut(s) 163, 358, 370, 427
SinI GGWCC 2 cut(s) 292, 331
SmiMI CAYNNNNRTG 2 cut(s) 105, 524
Sse9I AATT 1 cut(s) 188
SsiI CCGC 2 cut(s) 416, 469
SspMI CTAG 1 cut(s) 86
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 429, 457
TaaI ACNGT 3 cut(s) 199, 224, 316
TaiI ACGT 5 cut(s) 105, 399, 468, 477, 511
TaqI TCGA 1 cut(s) 364
TasI AATT 1 cut(s) 188
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 3 cut(s) 261, 319, 526
TseFI GTSAC 2 cut(s) 274, 548
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 2 cut(s) 274, 548
TspDTI ATGAA 1 cut(s) 281
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 3 cut(s) 261, 319, 526
Tth111I GACNNNGTC 1 cut(s) 341
VpaK11BI GGWCC 2 cut(s) 292, 331
XmiI GTMKAC 1 cut(s) 533
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.