AT5G66530

Glucose-6-phosphate 1-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008798
Physical Location & Seq
Reverse (-)
26551755 .. 26555886
4132 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66530.2

Sequence Viewer

Length: 924 bp
ATGGCGATTGTTTCCGTCTCTAACTCTTTTCTCACTTTCAATTCTCCCAATCAGCTCCGATTTAGCCGAAGAAGATTCTCTGCCATGGCTTCTTCAACTACTGGAGTGAGAGTCGCTGAAGGAGAAGGCAATTTGCCAAAACTTGTCCTTACTTCTCCTCAGAACAGCGAGGCTGAGATATATCTCTTCGGAGGCTGCATTACTTCTTGGAAAGTTGCGAGCGGTAAAGATCTTCTTTTTGTCAGACCAGATGCTGTCTTCAATAAGATTAAGCCCATTAGCGGAGGGATTCCACATTGTTTTCCACAGTTTGGACCTGGGCTAATTCAACAGCATGGGTTTGGAAGGAACATGGACTGGTCTGTTGTCGATTCCCAGAATGCAGATGACAATGCTGCTGTTACTCTTGAGCTTAAGGATGGTCCCTATAGTCGAGCCATGTGGGACTTTGCTTTCCAGGCTCTATACAAGGTCATTGTTGGCGCGGACTCCCTTTCCACTGAGCTAAAGATTACAAACACAGACGATAAACCATTTTCTTTCAGCACTGCGTTGCATACTTACTTCCGTGCTTCTTCTGCGGGGGCCTCCGTGAGAGGTCTAAAGGGTTGTAAAACTCTCAATAAGGATCCAGACCCTAAGAACCCAATAGAGGGTAAAGAAGACAGGGATGCAGTCACTTTTCCTGGATTTGTGGATACCGTCTATCTTGATGCTCCCAATGAATTGCAGTTTGATAATGGCTTGGGTGATAAAATAATCATCAAAAACACAAATTGGTCGGATGCGGTCTTGTGGAACCCGCATACTCAGATGGAGGCTTGTTACAGAGACTTTGTGTGCGTGGAAAATGCAAAGCTTGGGGATGTCAAGCTAGAGCCGGGACAGTCTTGGACTGCAACACAACTTCTCAGCATCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

307

Amino Acids

33.69

Weight (kDa)

5.64

Isoelectric Point (pI)

23.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 47 - 304 6.4e-45 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 311
AccBSI CCGCTC 1 cut(s) 222
AccII CGCG 1 cut(s) 485
AciI CCGC 6 cut(s) 222, 282, 485, 581, 788, 803
AclWI GGATC 2 cut(s) 623, 636
AcuI CTGAAG 1 cut(s) 138
AfiI CCNNNNNNNGG 4 cut(s) 281, 311, 652, 653
AflII CTTAAG 1 cut(s) 413
AgsI TTSAA 4 cut(s) 40, 96, 262, 329
AjnI CCWGG 3 cut(s) 316, 456, 685
AluBI AGCT 5 cut(s) 55, 412, 505, 859, 874
AluI AGCT 5 cut(s) 55, 412, 505, 859, 874
Alw26I GTCTC 2 cut(s) 22, 825
AlwI GGATC 2 cut(s) 623, 636
AlwNI CAGNNNCTG 1 cut(s) 254
AoxI GGCC 1 cut(s) 585
ApeKI GCWGC 2 cut(s) 195, 395
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 3 cut(s) 314, 422, 585
AsuC2I CCSGG 1 cut(s) 882
AsuHPI GGTGA 1 cut(s) 761
AvaII GGWCC 2 cut(s) 314, 422
BamHI GGATCC 1 cut(s) 628
BbsI GAAGAC 2 cut(s) 250, 669
BbvI GCAGC 2 cut(s) 182, 382
BccI CCATC 2 cut(s) 413, 808
BciT130I CCWGG 3 cut(s) 318, 458, 687
BciVI GTATCC 1 cut(s) 691
BcnI CCSGG 1 cut(s) 882
BcoDI GTCTC 2 cut(s) 22, 825
BfaI CTAG 1 cut(s) 875
BfmI CTRYAG 1 cut(s) 427
BfrI CTTAAG 1 cut(s) 413
BfuI GTATCC 1 cut(s) 691
BglII AGATCT 1 cut(s) 229
BisI GCNGC 2 cut(s) 196, 396
BlsI GCNGC 2 cut(s) 197, 397
Bme1390I CCNGG 4 cut(s) 318, 458, 687, 882
Bme18I GGWCC 2 cut(s) 314, 422
BmgT120I GGNCC 3 cut(s) 314, 422, 585
BmiI GGNNCC 4 cut(s) 424, 586, 630, 800
BmrFI CCNGG 4 cut(s) 318, 458, 687, 882
BmsI GCATC 4 cut(s) 241, 661, 703, 775
BpiI GAAGAC 2 cut(s) 250, 669
BpmI CTGGAG 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 1 cut(s) 882
BsaJI CCNNGG 2 cut(s) 84, 317
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 311, 652, 653
Bse1I ACTGG 2 cut(s) 106, 362
BseBI CCWGG 3 cut(s) 318, 458, 687
BseDI CCNNGG 2 cut(s) 84, 317
BseGI GGATG 4 cut(s) 424, 676, 790, 871
BseLI CCNNNNNNNGG 4 cut(s) 281, 311, 652, 653
BseMII CTCAG 4 cut(s) 165, 173, 492, 824
BseNI ACTGG 2 cut(s) 106, 362
BseRI GAGGAG 1 cut(s) 147
BseXI GCAGC 2 cut(s) 182, 382
Bsh1236I CGCG 1 cut(s) 485
BshFI GGCC 1 cut(s) 587
BsiSI CCGG 1 cut(s) 881
BslFI GGGAC 3 cut(s) 408, 458, 897
BslI CCNNNNNNNGG 4 cut(s) 281, 311, 652, 653
BsmAI GTCTC 2 cut(s) 22, 825
BsmBI CGTCTC 1 cut(s) 22
BsmFI GGGAC 3 cut(s) 408, 458, 897
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 1 cut(s) 587
Bsp143I GATC 2 cut(s) 229, 628
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 6 cut(s) 222, 282, 485, 581, 788, 803
BspANI GGCC 1 cut(s) 587
BspCNI CTCAG 5 cut(s) 166, 172, 493, 823, 924
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 4 cut(s) 424, 586, 630, 800
BspPI GGATC 2 cut(s) 623, 636
BspTI CTTAAG 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 222
BsrI ACTGG 2 cut(s) 106, 362
BssECI CCNNGG 2 cut(s) 84, 317
BssMI GATC 2 cut(s) 229, 628
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 3 cut(s) 318, 458, 687
Bst4CI ACNGT 3 cut(s) 309, 703, 888
Bst6I CTCTTC 1 cut(s) 191
BstAFI CTTAAG 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 6 cut(s) 159, 174, 501, 639, 810, 911
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 4 cut(s) 424, 676, 790, 871
BstFNI CGCG 1 cut(s) 485
BstHHI GCGC 1 cut(s) 485
BstKTI GATC 2 cut(s) 232, 631
BstMAI GTCTC 2 cut(s) 22, 825
BstMBI GATC 2 cut(s) 229, 628
BstMWI GCNNNNNNNGC 2 cut(s) 458, 578
BstNI CCWGG 3 cut(s) 318, 458, 687
BstSCI CCNGG 4 cut(s) 316, 456, 685, 880
BstSFI CTRYAG 1 cut(s) 427
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 2 cut(s) 182, 382
BstV2I GAAGAC 2 cut(s) 250, 669
BstX2I RGATCY 2 cut(s) 229, 628
BstYI RGATCY 2 cut(s) 229, 628
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 1 cut(s) 587
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 4 cut(s) 424, 676, 790, 871
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 2 cut(s) 498, 546
Cac8I GCNNGC 1 cut(s) 220
CaiI CAGNNNCTG 1 cut(s) 254
CfoI GCGC 1 cut(s) 485
Cfr13I GGNCC 3 cut(s) 314, 422, 585
CviAII CATG 4 cut(s) 85, 335, 352, 439
DdeI CTNAG 6 cut(s) 159, 174, 501, 639, 810, 911
DpnI GATC 2 cut(s) 231, 630
DpnII GATC 2 cut(s) 229, 628
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 84
Eco47I GGWCC 2 cut(s) 314, 422
Eco57I CTGAAG 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 585
EcoRII CCWGG 3 cut(s) 316, 456, 685
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 4 cut(s) 88, 338, 355, 442
FaiI YATR 9 cut(s) 86, 181, 336, 353, 429, 440, 466, 558, 807
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FaqI GGGAC 3 cut(s) 408, 458, 897
FatI CATG 4 cut(s) 84, 334, 351, 438
FauI CCCGC 2 cut(s) 574, 810
Fnu4HI GCNGC 2 cut(s) 196, 396
FokI GGATG 4 cut(s) 431, 683, 797, 878
Fsp4HI GCNGC 2 cut(s) 196, 396
FspBI CTAG 1 cut(s) 875
GlaI GCGC 1 cut(s) 484
GluI GCNGC 2 cut(s) 196, 396
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 1 cut(s) 587
HapII CCGG 1 cut(s) 881
HhaI GCGC 1 cut(s) 485
Hin1II CATG 4 cut(s) 88, 338, 355, 442
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 5 cut(s) 75, 111, 289, 371, 488
HpaII CCGG 1 cut(s) 881
HphI GGTGA 1 cut(s) 761
Hpy188I TCNGA 6 cut(s) 59, 162, 191, 245, 784, 813
Hpy188III TCNNGA 3 cut(s) 407, 632, 710
HpyAV CCTTC 3 cut(s) 113, 119, 339
HpyCH4III ACNGT 3 cut(s) 309, 703, 888
HpyCH4V TGCA 7 cut(s) 198, 383, 556, 674, 730, 854, 899
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 578
HpyF3I CTNAG 6 cut(s) 159, 174, 501, 639, 810, 911
Hsp92II CATG 4 cut(s) 88, 338, 355, 442
HspAI GCGC 1 cut(s) 483
Kzo9I GATC 2 cut(s) 229, 628
LmnI GCTCC 2 cut(s) 60, 721
Lsp1109I GCAGC 2 cut(s) 182, 382
LweI GCATC 4 cut(s) 241, 661, 703, 775
MaeI CTAG 1 cut(s) 875
MaeIII GTNAC 3 cut(s) 400, 676, 824
MalI GATC 2 cut(s) 231, 630
MbiI CCGCTC 1 cut(s) 222
MboI GATC 2 cut(s) 229, 628
MboII GAAGA 8 cut(s) 81, 84, 84, 178, 224, 250, 567, 674
MflI RGATCY 2 cut(s) 229, 628
MluCI AATT 5 cut(s) 40, 130, 324, 725, 775
MlyI GAGTC 2 cut(s) 120, 482
MmeI TCCRAC 1 cut(s) 762
MnlI CCTC 8 cut(s) 163, 168, 185, 278, 590, 598, 646, 811
MseI TTAA 2 cut(s) 270, 414
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 1 cut(s) 881
MspR9I CCNGG 4 cut(s) 318, 458, 687, 882
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 3 cut(s) 318, 458, 687
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 2 cut(s) 458, 578
NciI CCSGG 1 cut(s) 882
NcoI CCATGG 1 cut(s) 84
NdeII GATC 2 cut(s) 229, 628
NlaIII CATG 4 cut(s) 88, 338, 355, 442
NlaIV GGNNCC 4 cut(s) 424, 586, 630, 800
NmuCI GTSAC 1 cut(s) 676
PctI GAATGC 1 cut(s) 385
PfeI GAWTC 3 cut(s) 75, 289, 371
PflMI CCANNNNNTGG 1 cut(s) 311
PfoI TCCNGGA 1 cut(s) 685
PkrI GCNGC 2 cut(s) 197, 397
PleI GAGTC 2 cut(s) 119, 482
PpsI GAGTC 2 cut(s) 119, 482
Psp6I CCWGG 3 cut(s) 316, 456, 685
PspGI CCWGG 3 cut(s) 316, 456, 685
PspN4I GGNNCC 4 cut(s) 424, 586, 630, 800
PspPI GGNCC 3 cut(s) 314, 422, 585
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 2 cut(s) 229, 628
SaqAI TTAA 2 cut(s) 270, 414
SatI GCNGC 2 cut(s) 196, 396
Sau3AI GATC 2 cut(s) 229, 628
Sau96I GGNCC 3 cut(s) 314, 422, 585
SchI GAGTC 2 cut(s) 120, 482
ScrFI CCNGG 4 cut(s) 318, 458, 687, 882
SetI ASST 8 cut(s) 57, 319, 414, 474, 507, 601, 861, 876
SfaNI GCATC 4 cut(s) 241, 661, 703, 775
SfcI CTRYAG 1 cut(s) 427
SinI GGWCC 2 cut(s) 314, 422
SmlI CTYRAG 2 cut(s) 407, 413
SmoI CTYRAG 2 cut(s) 407, 413
Sse9I AATT 5 cut(s) 40, 130, 324, 725, 775
SsiI CCGC 6 cut(s) 222, 282, 485, 581, 788, 803
SspMI CTAG 1 cut(s) 875
StyD4I CCNGG 4 cut(s) 316, 456, 685, 880
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 3 cut(s) 309, 703, 888
TaqI TCGA 2 cut(s) 369, 433
TasI AATT 5 cut(s) 40, 130, 324, 725, 775
TfiI GAWTC 3 cut(s) 75, 289, 371
Tru1I TTAA 2 cut(s) 270, 414
Tru9I TTAA 2 cut(s) 270, 414
TscAI CASTG 2 cut(s) 505, 553
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 2 cut(s) 195, 395
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 1 cut(s) 738
TspGWI ACGGA 3 cut(s) 4, 557, 580
TspRI CASTG 2 cut(s) 505, 553
Van91I CCANNNNNTGG 1 cut(s) 311
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 314, 422
XspI CTAG 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.