MD02G1144900.v1.1

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008798
Physical Location & Seq
Reverse (-)
11983302 .. 11986404
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1144900.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCGATGGCGATGGTTTCTATGCTCGTTTCGCTCCCCACCCTCACTCCTCCCAACTCCCGCCGAGTCAACCGGTTTTGGCAAACAACGCCAAAAACGTCGTCATCCGTGATGGCTGTTGCAAGCGGCGCCGGAAGCAAAGAGAGCGTTACTGCAGGGGTGAAATTTGCAGAGGGAGAAGAGAAATTACCCAAGGTCGTTTTGACCAGCGCCGGCGGCAGTGAGGCGGATGTATATTTATATGGCGGTGTTATTACTTCTTGGAAAGTCCCAAATGGGAGGGACCTGCTTTTTGTTCGACCTGATGCGGTGTTCAACAAGAAGAAACCAATCAGTGGAGGCGTTCCACATTGTTTTCCACAGTTTGGACCAGGCCCTATGCAGCAGCACGGATTTGCAAGAAATCTGGACTGGTCCGTCATTGATTCTGAAAATGTGCAAGGAGATCCTACCATAACTTTAGAACTAAAGGATGGTGAATACAGTCGCTCAATGTGGGATTTCAGCTTTCAAGCTTTGTACAAGGTCACTTTGCAAACAAAGAACCTTTCCACTGAACTTGTAATTAAAAATACAGACAGCAAGCCATTTTCATTCAGCACCGCATTGCATACATACTTCCGGGCTGCTGTACAAGGGGCATCAGTTAAAGGTTTGAAAGGCTGCAAAACACTAAATAAAGATCCTGATCCCAAGAATCCACTGGAGGGCAAGGAAGAAAGGGATGGGATCACTTTTCCTGGATTCGTGGATTGTGTATACCTGGATGCCCCTAATGAGTTACATCTTGATAATGGCTTAGGTGATGTAATATCTATCAAGAACACAAATTGGACAGATGCTGTTTTGTGGAACCCATATCTGACAATGGAAGCATGCTACAAAGATTTTGTTTGTGTTGAAAATGCAAAGATTGGAAACGTACAGCTAGAGCCTGGTAATACGTGGATTGCTACGCAACATCTTAGCATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

326

Amino Acids

35.62

Weight (kDa)

6.24

Isoelectric Point (pI)

22.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 65 - 322 5.8e-45 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 294
AccB1I GGYRCC 1 cut(s) 128
AccB7I CCANNNNNTGG 2 cut(s) 335, 365
AccI GTMKAC 1 cut(s) 759
AciI CCGC 7 cut(s) 61, 126, 216, 227, 246, 308, 603
AclWI GGATC 4 cut(s) 440, 677, 683, 737
AcsI RAATTY 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 3 cut(s) 521, 633, 924
AfiI CCNNNNNNNGG 3 cut(s) 335, 365, 707
AgeI ACCGGT 1 cut(s) 72
AgsI TTSAA 4 cut(s) 316, 512, 658, 902
AjnI CCWGG 4 cut(s) 370, 739, 762, 934
AluBI AGCT 3 cut(s) 507, 515, 928
AluI AGCT 3 cut(s) 507, 515, 928
AlwI GGATC 4 cut(s) 440, 677, 683, 737
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 1 cut(s) 373
ApeKI GCWGC 4 cut(s) 382, 385, 626, 663
ApoI RAATTY 1 cut(s) 164
ArsI GACNNNNNNTTYG 4 cut(s) 86, 118, 275, 307
AseI ATTAAT 1 cut(s) 972
AsiGI ACCGGT 1 cut(s) 72
AspLEI GCGC 2 cut(s) 131, 212
AspS9I GGNCC 4 cut(s) 283, 368, 374, 414
AsuC2I CCSGG 1 cut(s) 623
AsuHPI GGTGA 3 cut(s) 172, 488, 815
AvaII GGWCC 3 cut(s) 283, 368, 414
BanI GGYRCC 1 cut(s) 128
BarI GAAGNNNNNNTAC 2 cut(s) 171, 203
BbvI GCAGC 4 cut(s) 394, 397, 613, 650
BccI CCATC 4 cut(s) 7, 106, 467, 719
BciT130I CCWGG 4 cut(s) 372, 741, 764, 936
BcnI CCSGG 1 cut(s) 623
BfaI CTAG 1 cut(s) 929
BfmI CTRYAG 1 cut(s) 153
BfoI RGCGCY 2 cut(s) 132, 213
BfuAI ACCTGC 1 cut(s) 294
BisI GCNGC 6 cut(s) 127, 217, 383, 386, 627, 664
BlsI GCNGC 6 cut(s) 128, 218, 384, 387, 628, 665
Bme1390I CCNGG 5 cut(s) 372, 623, 741, 764, 936
Bme18I GGWCC 3 cut(s) 283, 368, 414
BmgT120I GGNCC 4 cut(s) 283, 368, 374, 414
BmiI GGNNCC 3 cut(s) 130, 284, 854
BmrFI CCNGG 5 cut(s) 372, 623, 741, 764, 936
BmsI GCATC 4 cut(s) 295, 650, 757, 829
BpmI CTGGAG 1 cut(s) 725
Bpu10I CCTNAGC 1 cut(s) 799
BpuMI CCSGG 1 cut(s) 623
BsaAI YACGTR 1 cut(s) 945
BsaBI GATNNNNATC 1 cut(s) 687
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 192
BsaWI WCCGGW 1 cut(s) 72
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 3 cut(s) 335, 365, 707
Bse118I RCCGGY 2 cut(s) 72, 212
Bse1I ACTGG 2 cut(s) 416, 708
Bse3DI GCAATG 1 cut(s) 605
Bse8I GATNNNNATC 1 cut(s) 687
BseBI CCWGG 4 cut(s) 372, 741, 764, 936
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 5 cut(s) 104, 235, 478, 730, 772
BseJI GATNNNNATC 1 cut(s) 687
BseLI CCNNNNNNNGG 3 cut(s) 335, 365, 707
BseMI GCAATG 1 cut(s) 605
BseNI ACTGG 2 cut(s) 416, 708
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 4 cut(s) 394, 397, 613, 650
BshFI GGCC 1 cut(s) 375
BshNI GGYRCC 1 cut(s) 128
BshTI ACCGGT 1 cut(s) 72
BsiSI CCGG 4 cut(s) 73, 132, 213, 622
BslFI GGGAC 2 cut(s) 254, 296
BslI CCNNNNNNNGG 3 cut(s) 335, 365, 707
BsmFI GGGAC 2 cut(s) 254, 296
BsnI GGCC 1 cut(s) 375
Bsp1407I TGTACA 2 cut(s) 519, 631
Bsp143I GATC 4 cut(s) 445, 682, 688, 729
BspACI CCGC 7 cut(s) 61, 126, 216, 227, 246, 308, 603
BspANI GGCC 1 cut(s) 375
BspLI GGNNCC 3 cut(s) 130, 284, 854
BspMAI CTGCAG 1 cut(s) 157
BspMI ACCTGC 1 cut(s) 294
BspPI GGATC 4 cut(s) 440, 677, 683, 737
BspT107I GGYRCC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 605
BsrFI RCCGGY 2 cut(s) 72, 212
BsrGI TGTACA 2 cut(s) 519, 631
BsrI ACTGG 2 cut(s) 416, 708
BssAI RCCGGY 2 cut(s) 72, 212
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 4 cut(s) 445, 682, 688, 729
BssNAI GTATAC 1 cut(s) 760
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 192
Bst1107I GTATAC 1 cut(s) 760
Bst2UI CCWGG 4 cut(s) 372, 741, 764, 936
Bst4CI ACNGT 2 cut(s) 363, 485
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 129
BstAUI TGTACA 2 cut(s) 519, 631
BstBAI YACGTR 1 cut(s) 945
BstC8I GCNNGC 4 cut(s) 124, 214, 584, 877
BstDEI CTNAG 2 cut(s) 799, 965
BstF5I GGATG 5 cut(s) 104, 235, 478, 730, 772
BstH2I RGCGCY 2 cut(s) 132, 213
BstHHI GCGC 2 cut(s) 131, 212
BstKTI GATC 4 cut(s) 448, 685, 691, 732
BstMBI GATC 4 cut(s) 445, 682, 688, 729
BstMWI GCNNNNNNNGC 6 cut(s) 31, 88, 128, 135, 144, 216
BstNI CCWGG 4 cut(s) 372, 741, 764, 936
BstNSI RCATGY 1 cut(s) 879
BstSCI CCNGG 5 cut(s) 370, 621, 739, 762, 934
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 4 cut(s) 394, 397, 613, 650
BstX2I RGATCY 2 cut(s) 445, 682
BstYI RGATCY 2 cut(s) 445, 682
BstZ17I GTATAC 1 cut(s) 760
BsuRI GGCC 1 cut(s) 375
BtgZI GCGATG 2 cut(s) 20, 26
BtsCI GGATG 5 cut(s) 104, 235, 478, 730, 772
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 4 cut(s) 226, 340, 552, 701
BveI ACCTGC 1 cut(s) 294
Cac8I GCNNGC 4 cut(s) 124, 214, 584, 877
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 2 cut(s) 131, 212
Cfr10I RCCGGY 2 cut(s) 72, 212
Cfr13I GGNCC 4 cut(s) 283, 368, 374, 414
Csp6I GTAC 3 cut(s) 520, 632, 923
CspAI ACCGGT 1 cut(s) 72
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviAII CATG 1 cut(s) 876
CviQI GTAC 3 cut(s) 520, 632, 923
DdeI CTNAG 2 cut(s) 799, 965
DinI GGCGCC 1 cut(s) 130
DpnI GATC 4 cut(s) 447, 684, 690, 731
DpnII GATC 4 cut(s) 445, 682, 688, 729
DrdI GACNNNNNNGTC 1 cut(s) 416
DseDI GACNNNNNNGTC 1 cut(s) 416
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 192
Eco47I GGWCC 3 cut(s) 283, 368, 414
EcoO109I RGGNCCY 2 cut(s) 283, 374
EcoRII CCWGG 4 cut(s) 370, 739, 762, 934
EcoT14I CCWWGG 1 cut(s) 192
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 1 cut(s) 879
FaqI GGGAC 2 cut(s) 254, 296
FatI CATG 1 cut(s) 875
FauI CCCGC 1 cut(s) 68
FblI GTMKAC 1 cut(s) 759
Fnu4HI GCNGC 6 cut(s) 127, 217, 383, 386, 627, 664
FokI GGATG 5 cut(s) 91, 242, 485, 737, 779
Fsp4HI GCNGC 6 cut(s) 127, 217, 383, 386, 627, 664
FspBI CTAG 1 cut(s) 929
GlaI GCGC 2 cut(s) 130, 211
GluI GCNGC 6 cut(s) 127, 217, 383, 386, 627, 664
GsuI CTGGAG 1 cut(s) 725
HaeII RGCGCY 2 cut(s) 132, 213
HaeIII GGCC 1 cut(s) 375
HapII CCGG 4 cut(s) 73, 132, 213, 622
HhaI GCGC 2 cut(s) 131, 212
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 1 cut(s) 879
Hin6I GCGC 2 cut(s) 129, 210
HinP1I GCGC 2 cut(s) 129, 210
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 513
HinfI GANTC 4 cut(s) 66, 425, 697, 744
HpaII CCGG 4 cut(s) 73, 132, 213, 622
HphI GGTGA 3 cut(s) 172, 488, 815
Hpy166II GTNNAC 2 cut(s) 70, 760
Hpy188I TCNGA 2 cut(s) 430, 864
Hpy188III TCNNGA 4 cut(s) 407, 686, 788, 820
Hpy8I GTNNAC 2 cut(s) 70, 760
Hpy99I CGWCG 1 cut(s) 103
HpyCH4III ACNGT 2 cut(s) 363, 485
HpyCH4IV ACGT 3 cut(s) 98, 921, 944
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 88, 128, 135, 144, 216
HpyF3I CTNAG 2 cut(s) 799, 965
HpySE526I ACGT 3 cut(s) 98, 921, 944
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 1 cut(s) 879
HspAI GCGC 2 cut(s) 129, 210
KasI GGCGCC 1 cut(s) 128
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Kzo9I GATC 4 cut(s) 445, 682, 688, 729
LmnI GCTCC 1 cut(s) 39
Lsp1109I GCAGC 4 cut(s) 394, 397, 613, 650
LweI GCATC 4 cut(s) 295, 650, 757, 829
MaeI CTAG 1 cut(s) 929
MaeII ACGT 3 cut(s) 98, 921, 944
MaeIII GTNAC 3 cut(s) 148, 526, 780
MalI GATC 4 cut(s) 447, 684, 690, 731
MboI GATC 4 cut(s) 445, 682, 688, 729
MboII GAAGA 3 cut(s) 191, 334, 728
MflI RGATCY 2 cut(s) 445, 682
MluCI AATT 5 cut(s) 164, 185, 564, 829, 973
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 1 cut(s) 75
MnlI CCTC 7 cut(s) 53, 60, 166, 217, 273, 332, 700
MreI CGCCGGCG 1 cut(s) 212
MroNI GCCGGC 1 cut(s) 212
MseI TTAA 3 cut(s) 567, 648, 972
MspI CCGG 4 cut(s) 73, 132, 213, 622
MspR9I CCNGG 5 cut(s) 372, 623, 741, 764, 936
MvaI CCWGG 4 cut(s) 372, 741, 764, 936
MwoI GCNNNNNNNGC 6 cut(s) 31, 88, 128, 135, 144, 216
NaeI GCCGGC 1 cut(s) 214
NarI GGCGCC 1 cut(s) 129
NciI CCSGG 1 cut(s) 623
NdeII GATC 4 cut(s) 445, 682, 688, 729
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 1 cut(s) 879
NlaIV GGNNCC 3 cut(s) 130, 284, 854
NmeAIII GCCGAG 1 cut(s) 89
NmuCI GTSAC 1 cut(s) 526
NspI RCATGY 1 cut(s) 879
PaeI GCATGC 1 cut(s) 879
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 3 cut(s) 425, 697, 744
PflMI CCANNNNNTGG 2 cut(s) 335, 365
PfoI TCCNGGA 1 cut(s) 739
PinAI ACCGGT 1 cut(s) 72
PkrI GCNGC 6 cut(s) 128, 218, 384, 387, 628, 665
PleI GAGTC 1 cut(s) 74
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 74
Ppu21I YACGTR 1 cut(s) 945
PpuMI RGGWCCY 1 cut(s) 283
PshBI ATTAAT 1 cut(s) 972
Psp5II RGGWCCY 1 cut(s) 283
Psp6I CCWGG 4 cut(s) 370, 739, 762, 934
PspGI CCWGG 4 cut(s) 370, 739, 762, 934
PspN4I GGNNCC 3 cut(s) 130, 284, 854
PspPI GGNCC 4 cut(s) 283, 368, 374, 414
PspPPI RGGWCCY 1 cut(s) 283
PstI CTGCAG 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 2 cut(s) 445, 682
RsaI GTAC 3 cut(s) 521, 633, 924
RsaNI GTAC 3 cut(s) 520, 632, 923
SaqAI TTAA 3 cut(s) 567, 648, 972
SatI GCNGC 6 cut(s) 127, 217, 383, 386, 627, 664
Sau3AI GATC 4 cut(s) 445, 682, 688, 729
Sau96I GGNCC 4 cut(s) 283, 368, 374, 414
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 5 cut(s) 372, 623, 741, 764, 936
SfaNI GCATC 4 cut(s) 295, 650, 757, 829
SfcI CTRYAG 1 cut(s) 153
SfoI GGCGCC 1 cut(s) 130
SgrAI CRCCGGYG 1 cut(s) 212
SinI GGWCC 3 cut(s) 283, 368, 414
SphI GCATGC 1 cut(s) 879
Sse9I AATT 5 cut(s) 164, 185, 564, 829, 973
SsiI CCGC 7 cut(s) 61, 126, 216, 227, 246, 308, 603
SspDI GGCGCC 1 cut(s) 128
SspMI CTAG 1 cut(s) 929
StyD4I CCNGG 5 cut(s) 370, 621, 739, 762, 934
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 2 cut(s) 363, 485
TaiI ACGT 3 cut(s) 101, 924, 947
TaqI TCGA 1 cut(s) 298
TasI AATT 5 cut(s) 164, 185, 564, 829, 973
TatI WGTACW 2 cut(s) 519, 631
TauI GCSGC 2 cut(s) 129, 219
TfiI GAWTC 3 cut(s) 425, 697, 744
Tru1I TTAA 3 cut(s) 567, 648, 972
Tru9I TTAA 3 cut(s) 567, 648, 972
TscAI CASTG 4 cut(s) 226, 340, 559, 708
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 4 cut(s) 382, 385, 626, 663
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 1 cut(s) 582
TspGWI ACGGA 3 cut(s) 97, 405, 406
TspRI CASTG 4 cut(s) 226, 340, 559, 708
Van91I CCANNNNNTGG 2 cut(s) 335, 365
VpaK11BI GGWCC 3 cut(s) 283, 368, 414
VspI ATTAAT 1 cut(s) 972
XapI RAATTY 1 cut(s) 164
XceI RCATGY 1 cut(s) 879
XcmI CCANNNNNNNNNTGG 1 cut(s) 700
XmiI GTMKAC 1 cut(s) 759
XspI CTAG 1 cut(s) 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.