AT5G67050

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003099
Physical Location & Seq
Reverse (-)
26759378 .. 26761197
1820 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67050.1

Sequence Viewer

Length: 1434 bp
ATGATGAACAGCGACGATGACGATGAACCGAGAGGATATCTGATTCTCCGACCGGAAGAGCTCCGGCCATGGGAACTTGTTCGTTTGTTATTCTCCGGAGATATAGAGAAACCGAGATCAGTGGATAGCTCGGAAACCGAAGAACACAGTTTCCGACATCGATGGCTTATCTTTGTCTCTCTCGTCCTTCTCAAGCTTCTTCGATTCTTCTCCAAGCTTTTAGCTCTCGTTGGCTCCGCCTTAGAATTCTCTTTAAACTTCCTCTCCAATAACTCTTTCTCCGGTCTCTTCCTTCGAGGAGAAGTGGTGATGCCGCAACGGACATCGGAGAATTATCAGTCATTTATCGGACATTTAGACACGAGGGTAAGTTTAGACATGACGTTGAATCGTGAAGATGGAGAGAAATACTATGCTGCATTGTCCATTATGGCTTCAAAAATTGCCTACGAAAACGCAGCCCGTATTAAACATGTCGTCGAGAATCACTGGAATATGAAGTATTTAGGTCTCGTTGACTATTGGAACGAGTACCAAGAAAAGGAAACAACACAAGCCTTTATAATGTCTACTGACGAAACAACAACAAGATCCAACGGTCAAGAAACGACGGTGGTGGTTGCATTTAGAGGCACAGAACTGTTTAATTCCGAAGATTGGTGTTCAGATTTCGACATTACATGGTTTGAGCTCCCAAACATTGGGAATATCCATGGTGGTTTCATGAAAGCTCTAGGGCTTCAAAACAATTGTAGTTGGCCTAAAGAACCTCTCTCAAACCCTGATCGAAAATCCCCTTTAGCTTACTACTCAATAAGAGACTCTTTGAAAACTCTGATCGCTCAAAACAAAAACACCAAGTTTGTTCTGACCGGTCATAGCTTAGGAGGAGCCCTCGCGATATTGTTCACGGCTGTGCTTGTGATTCACCACGAGACCGAGTTGTTGGAGAGGATTCAAGGGGTGTATACTTATGGACAGCCTAGGGTTGGAGATTCCAAGTTTGGTGAATTTATGGAGAAGAAATTAGAAAAATATAATATAAAGTATTATAGATTTGTTTATAATAACGACATTGTTCCAAGATTGCCGTACGATGATAAGGATTTGATGTTCAAACACTTTGGCACTTGCATCTACTACGACCAGAACTATCAAGCAAAGGTAATGAGAGAGCAATCGGACGAAAACTTTTTCTTGTTGCGAGGGATTATAAAGATGATGTGGAGTGCTATATTGGAGTTTATAAGAAGCTTCACTATAGTGGCTGAGAAGGGCTCGGAGTACAGTGAAGGATGGTTGTTGAAGGGTGGTAGGGCTTTGGGGATTATAGTCCCTGGTGTTTCAAATCATACACCTCAAGATTATGTGAATGCCACTAGATTAACCCTTCCTTGTGTTTTCCAAGTCTATAGAGATGTATCCATAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

477

Amino Acids

55.18

Weight (kDa)

5.76

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 206 - 366 1.4e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 563, 1065, 1214, 1247
AccB7I CCANNNNNTGG 1 cut(s) 701
AccI GTMKAC 2 cut(s) 569, 968
AccII CGCG 1 cut(s) 899
AccIII TCCGGA 1 cut(s) 95
AciI CCGC 2 cut(s) 237, 314
AclWI GGATC 1 cut(s) 585
AcoI YGGCCR 1 cut(s) 65
AcsI RAATTY 2 cut(s) 245, 1010
AfaI GTAC 3 cut(s) 533, 1094, 1286
AfiI CCNNNNNNNGG 5 cut(s) 70, 541, 657, 701, 989
AflIII ACRYGT 1 cut(s) 472
AgeI ACCGGT 1 cut(s) 872
AgsI TTSAA 8 cut(s) 388, 438, 743, 829, 959, 1117, 1306, 1347
AjnI CCWGG 1 cut(s) 1336
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AleI CACNNNNGTG 2 cut(s) 914, 1262
AloI GAACNNNNNNTCC 4 cut(s) 135, 167, 1097, 1129
Alw21I GWGCWC 2 cut(s) 63, 693
Alw26I GTCTC 5 cut(s) 181, 290, 515, 813, 929
AlwI GGATC 1 cut(s) 585
Aor13HI TCCGGA 1 cut(s) 95
AoxI GGCC 2 cut(s) 65, 758
ApeKI GCWGC 2 cut(s) 416, 458
ApoI RAATTY 2 cut(s) 245, 1010
AsiGI ACCGGT 1 cut(s) 872
Asp700I GAANNNNTTC 1 cut(s) 78
AspA2I CCTAGG 1 cut(s) 983
AsuHPI GGTGA 3 cut(s) 319, 920, 1019
AvrII CCTAGG 1 cut(s) 983
BanII GRGCYC 4 cut(s) 63, 693, 895, 1280
BauI CACGAG 2 cut(s) 361, 932
Bbv12I GWGCWC 2 cut(s) 63, 693
BbvI GCAGC 2 cut(s) 403, 470
BccI CCATC 3 cut(s) 156, 392, 1290
BceAI ACGGC 2 cut(s) 927, 1075
BciT130I CCWGG 1 cut(s) 1338
BciVI GTATCC 1 cut(s) 1432
BcoDI GTCTC 5 cut(s) 181, 290, 515, 813, 929
BfaI CTAG 3 cut(s) 734, 984, 1380
BfmI CTRYAG 2 cut(s) 1260, 1411
BfuI GTATCC 1 cut(s) 1432
BisI GCNGC 3 cut(s) 314, 417, 459
BlnI CCTAGG 1 cut(s) 983
BlsI GCNGC 3 cut(s) 315, 418, 460
Bme1390I CCNGG 1 cut(s) 1338
BmiI GGNNCC 2 cut(s) 235, 892
BmrFI CCNGG 1 cut(s) 1338
BmsI GCATC 2 cut(s) 300, 1143
BplI GAGNNNNNCTC 4 cut(s) 879, 911, 1262, 1294
Bpu10I CCTNAGC 1 cut(s) 883
BpuEI CTTGAG 2 cut(s) 176, 1344
Bsa29I ATCGAT 1 cut(s) 160
BsaI GGTCTC 3 cut(s) 290, 515, 929
BsaJI CCNNGG 4 cut(s) 68, 712, 983, 1336
BsaWI WCCGGW 4 cut(s) 52, 95, 281, 872
BsaXI ACNNNNNCTCC 6 cut(s) 248, 263, 278, 291, 293, 321
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 541, 657, 701, 989
Bse118I RCCGGY 1 cut(s) 872
Bse1I ACTGG 1 cut(s) 494
BseAI TCCGGA 1 cut(s) 95
BseBI CCWGG 1 cut(s) 1338
BseCI ATCGAT 1 cut(s) 160
BseDI CCNNGG 4 cut(s) 68, 712, 983, 1336
BseGI GGATG 1 cut(s) 1301
BseLI CCNNNNNNNGG 5 cut(s) 70, 541, 657, 701, 989
BseMII CTCAG 1 cut(s) 1260
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 2 cut(s) 312, 903
BseXI GCAGC 2 cut(s) 403, 470
Bsh1236I CGCG 1 cut(s) 899
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 67, 760
BshTI ACCGGT 1 cut(s) 872
BshVI ATCGAT 1 cut(s) 160
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 2 cut(s) 63, 693
BsiSI CCGG 5 cut(s) 53, 64, 96, 282, 873
BsiWI CGTACG 1 cut(s) 1092
BslFI GGGAC 1 cut(s) 1319
BslI CCNNNNNNNGG 5 cut(s) 70, 541, 657, 701, 989
BsmAI GTCTC 5 cut(s) 181, 290, 515, 813, 929
BsmFI GGGAC 1 cut(s) 1319
BsmI GAATGC 1 cut(s) 1378
BsnI GGCC 2 cut(s) 67, 760
Bso31I GGTCTC 3 cut(s) 290, 515, 929
Bsp1286I GDGCHC 4 cut(s) 63, 693, 895, 1280
Bsp13I TCCGGA 1 cut(s) 95
Bsp143I GATC 4 cut(s) 116, 590, 784, 837
Bsp19I CCATGG 2 cut(s) 68, 712
Bsp68I TCGCGA 1 cut(s) 899
BspACI CCGC 2 cut(s) 237, 314
BspANI GGCC 2 cut(s) 67, 760
BspCNI CTCAG 1 cut(s) 1261
BspDI ATCGAT 1 cut(s) 160
BspEI TCCGGA 1 cut(s) 95
BspFNI CGCG 1 cut(s) 899
BspHI TCATGA 1 cut(s) 723
BspLI GGNNCC 2 cut(s) 235, 892
BspPI GGATC 1 cut(s) 585
BspQI GCTCTTC 1 cut(s) 51
BspTNI GGTCTC 3 cut(s) 290, 515, 929
BsrFI RCCGGY 1 cut(s) 872
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 872
BssECI CCNNGG 4 cut(s) 68, 712, 983, 1336
BssMI GATC 4 cut(s) 116, 590, 784, 837
BssNAI GTATAC 1 cut(s) 969
BssSI CACGAG 2 cut(s) 361, 932
BssT1I CCWWGG 3 cut(s) 68, 712, 983
Bst1107I GTATAC 1 cut(s) 969
Bst2BI CACGAG 2 cut(s) 361, 932
Bst2UI CCWGG 1 cut(s) 1338
Bst4CI ACNGT 5 cut(s) 149, 599, 613, 642, 1289
Bst6I CTCTTC 2 cut(s) 51, 293
BstDEI CTNAG 3 cut(s) 241, 883, 1269
BstDSI CCRYGG 2 cut(s) 68, 712
BstF5I GGATG 1 cut(s) 1301
BstFNI CGCG 1 cut(s) 899
BstKTI GATC 4 cut(s) 119, 593, 787, 840
BstMAI GTCTC 5 cut(s) 181, 290, 515, 813, 929
BstMBI GATC 4 cut(s) 116, 590, 784, 837
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 1 cut(s) 1338
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 1336
BstSFI CTRYAG 2 cut(s) 1260, 1411
BstUI CGCG 1 cut(s) 899
BstV1I GCAGC 2 cut(s) 403, 470
BstX2I RGATCY 1 cut(s) 590
BstYI RGATCY 1 cut(s) 590
BstZ17I GTATAC 1 cut(s) 969
Bsu15I ATCGAT 1 cut(s) 160
BsuI GTATCC 1 cut(s) 1432
BsuRI GGCC 2 cut(s) 67, 760
BsuTUI ATCGAT 1 cut(s) 160
BtgI CCRYGG 2 cut(s) 68, 712
BtsCI GGATG 1 cut(s) 1301
BtsIMutI CAGTG 3 cut(s) 126, 487, 1294
BtuMI TCGCGA 1 cut(s) 899
CciI TCATGA 1 cut(s) 723
Cfr10I RCCGGY 1 cut(s) 872
ClaI ATCGAT 1 cut(s) 160
Csp6I GTAC 3 cut(s) 532, 1093, 1285
CspAI ACCGGT 1 cut(s) 872
CviAII CATG 6 cut(s) 69, 379, 473, 681, 713, 724
CviQI GTAC 3 cut(s) 532, 1093, 1285
DdeI CTNAG 3 cut(s) 241, 883, 1269
DpnI GATC 4 cut(s) 118, 592, 786, 839
DpnII GATC 4 cut(s) 116, 590, 784, 837
DraI TTTAAA 1 cut(s) 255
EaeI YGGCCR 1 cut(s) 65
Eam1104I CTCTTC 2 cut(s) 51, 293
EarI CTCTTC 2 cut(s) 51, 293
EciI GGCGGA 1 cut(s) 226
Ecl136II GAGCTC 2 cut(s) 61, 691
Eco130I CCWWGG 3 cut(s) 68, 712, 983
Eco24I GRGCYC 4 cut(s) 63, 693, 895, 1280
Eco31I GGTCTC 3 cut(s) 290, 515, 929
Eco32I GATATC 1 cut(s) 38
Eco53kI GAGCTC 2 cut(s) 61, 691
EcoICRI GAGCTC 2 cut(s) 61, 691
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 1336
EcoRV GATATC 1 cut(s) 38
EcoT14I CCWWGG 3 cut(s) 68, 712, 983
EcoT38I GRGCYC 4 cut(s) 63, 693, 895, 1280
ErhI CCWWGG 3 cut(s) 68, 712, 983
FaeI CATG 6 cut(s) 72, 382, 476, 684, 716, 727
FalI AAGNNNNNCTT 2 cut(s) 808, 840
FaqI GGGAC 1 cut(s) 1319
FatI CATG 6 cut(s) 68, 378, 472, 680, 712, 723
FblI GTMKAC 2 cut(s) 569, 968
Fnu4HI GCNGC 3 cut(s) 314, 417, 459
FokI GGATG 1 cut(s) 1308
FriOI GRGCYC 4 cut(s) 63, 693, 895, 1280
Fsp4HI GCNGC 3 cut(s) 314, 417, 459
FspBI CTAG 3 cut(s) 734, 984, 1380
GluI GCNGC 3 cut(s) 314, 417, 459
HaeIII GGCC 2 cut(s) 67, 760
HapII CCGG 5 cut(s) 53, 64, 96, 282, 873
Hin1II CATG 6 cut(s) 72, 382, 476, 684, 716, 727
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 3 cut(s) 194, 215, 1252
HinfI GANTC 8 cut(s) 43, 204, 388, 484, 821, 925, 955, 995
HpaII CCGG 5 cut(s) 53, 64, 96, 282, 873
HphI GGTGA 3 cut(s) 319, 920, 1019
Hpy166II GTNNAC 4 cut(s) 517, 570, 909, 969
Hpy188III TCNNGA 7 cut(s) 96, 392, 481, 602, 724, 898, 1361
Hpy8I GTNNAC 4 cut(s) 517, 570, 909, 969
Hpy99I CGWCG 3 cut(s) 17, 482, 613
HpyAV CCTTC 6 cut(s) 197, 302, 1267, 1286, 1300, 1400
HpyCH4III ACNGT 5 cut(s) 149, 599, 613, 642, 1289
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 3 cut(s) 419, 623, 1134
HpyF3I CTNAG 3 cut(s) 241, 883, 1269
HpySE526I ACGT 1 cut(s) 383
Hsp92II CATG 6 cut(s) 72, 382, 476, 684, 716, 727
Kpn2I TCCGGA 1 cut(s) 95
Kzo9I GATC 4 cut(s) 116, 590, 784, 837
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 4 cut(s) 66, 239, 696, 890
Lsp1109I GCAGC 2 cut(s) 403, 470
LweI GCATC 2 cut(s) 300, 1143
MaeI CTAG 3 cut(s) 734, 984, 1380
MaeII ACGT 1 cut(s) 383
MalI GATC 4 cut(s) 118, 592, 786, 839
MboI GATC 4 cut(s) 116, 590, 784, 837
MboII GAAGA 8 cut(s) 68, 152, 191, 199, 280, 407, 665, 1033
MfeI CAATTG 1 cut(s) 748
MflI RGATCY 1 cut(s) 590
MhlI GDGCHC 4 cut(s) 63, 693, 895, 1280
MluCI AATT 7 cut(s) 245, 331, 441, 646, 748, 1010, 1025
MlyI GAGTC 1 cut(s) 815
MmeI TCCRAC 5 cut(s) 73, 178, 618, 927, 970
MroI TCCGGA 1 cut(s) 95
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 4 cut(s) 254, 468, 645, 1385
MslI CAYNNNNRTG 2 cut(s) 914, 1262
MspI CCGG 5 cut(s) 53, 64, 96, 282, 873
MspR9I CCNGG 1 cut(s) 1338
MunI CAATTG 1 cut(s) 748
Mva1269I GAATGC 1 cut(s) 1378
MvaI CCWGG 1 cut(s) 1338
MvnI CGCG 1 cut(s) 899
NcoI CCATGG 2 cut(s) 68, 712
NdeII GATC 4 cut(s) 116, 590, 784, 837
NlaIII CATG 6 cut(s) 72, 382, 476, 684, 716, 727
NlaIV GGNNCC 2 cut(s) 235, 892
NruI TCGCGA 1 cut(s) 899
NspI RCATGY 1 cut(s) 476
OliI CACNNNNGTG 2 cut(s) 914, 1262
PagI TCATGA 1 cut(s) 723
PciI ACATGT 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 51
PctI GAATGC 1 cut(s) 1378
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 7 cut(s) 43, 204, 388, 484, 925, 955, 995
Pfl23II CGTACG 1 cut(s) 1092
PflMI CCANNNNNTGG 1 cut(s) 701
PinAI ACCGGT 1 cut(s) 872
PkrI GCNGC 3 cut(s) 315, 418, 460
PleI GAGTC 1 cut(s) 815
PpsI GAGTC 1 cut(s) 815
PscI ACATGT 1 cut(s) 472
PsiI TTATAA 4 cut(s) 563, 1065, 1214, 1247
Psp124BI GAGCTC 2 cut(s) 63, 693
Psp6I CCWGG 1 cut(s) 1336
PspGI CCWGG 1 cut(s) 1336
PspLI CGTACG 1 cut(s) 1092
PspN4I GGNNCC 2 cut(s) 235, 892
PsuI RGATCY 1 cut(s) 590
RruI TCGCGA 1 cut(s) 899
RsaI GTAC 3 cut(s) 533, 1094, 1286
RsaNI GTAC 3 cut(s) 532, 1093, 1285
RseI CAYNNNNRTG 2 cut(s) 914, 1262
SacI GAGCTC 2 cut(s) 63, 693
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 4 cut(s) 254, 468, 645, 1385
SatI GCNGC 3 cut(s) 314, 417, 459
Sau3AI GATC 4 cut(s) 116, 590, 784, 837
SchI GAGTC 1 cut(s) 815
ScrFI CCNGG 1 cut(s) 1338
SduI GDGCHC 4 cut(s) 63, 693, 895, 1280
SfaNI GCATC 2 cut(s) 300, 1143
SfcI CTRYAG 2 cut(s) 1260, 1411
SmiMI CAYNNNNRTG 2 cut(s) 914, 1262
SmlI CTYRAG 2 cut(s) 191, 1359
SmoI CTYRAG 2 cut(s) 191, 1359
Sse9I AATT 7 cut(s) 245, 331, 441, 646, 748, 1010, 1025
SsiI CCGC 2 cut(s) 237, 314
SspMI CTAG 3 cut(s) 734, 984, 1380
SstI GAGCTC 2 cut(s) 63, 693
StyD4I CCNGG 1 cut(s) 1336
StyI CCWWGG 3 cut(s) 68, 712, 983
TaaI ACNGT 5 cut(s) 149, 599, 613, 642, 1289
TaiI ACGT 1 cut(s) 386
TaqI TCGA 6 cut(s) 160, 202, 295, 480, 672, 787
TaqII GACCGA 1 cut(s) 953
TasI AATT 7 cut(s) 245, 331, 441, 646, 748, 1010, 1025
TatI WGTACW 1 cut(s) 1284
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 7 cut(s) 43, 204, 388, 484, 925, 955, 995
Tru1I TTAA 4 cut(s) 254, 468, 645, 1385
Tru9I TTAA 4 cut(s) 254, 468, 645, 1385
TscAI CASTG 3 cut(s) 126, 494, 1294
TseI GCWGC 2 cut(s) 416, 458
TspDTI ATGAA 5 cut(s) 20, 39, 512, 712, 740
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 3 cut(s) 126, 494, 1294
Van91I CCANNNNNTGG 1 cut(s) 701
XapI RAATTY 2 cut(s) 245, 1010
XceI RCATGY 1 cut(s) 476
XmaJI CCTAGG 1 cut(s) 983
XmiI GTMKAC 2 cut(s) 569, 968
XmnI GAANNNNTTC 1 cut(s) 78
XspI CTAG 3 cut(s) 734, 984, 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.