MD02G1173000.v1.1

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003099
Physical Location & Seq
Reverse (-)
15165276 .. 15168309
3034 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1173000.v1.1.491

Sequence Viewer

Length: 1119 bp
ATGGCGATGATTGGCAGGGGGATCGAGATCTGCTTGAACCTTTTCTCAAGTACTCAAAACTTGGTCGGACTGTTCAAAAATTTGCTGCGAGGTGAGGATATAGTACCAAAGAAAGACTCAAGAACGTTCTTGTCGTTCATTGGAAATTTGGACAAACGAGTGGAGCTGGACAAAAGCATCAAACATGGAGATGTCAGGTATAATGCAGCACTCAGTATGATGGCTTCTAAAATATCGTACGAGAACAAATTCTACATCGAAACTACAGTCCAAGATCACTGGAAGATGGAGTTCATCGGCTCTTACGACTTCCGGAATGATTATCAAGGAAAATCCACAACCCAAGCCTGTATTCTACGTGACAGAAATGCGGACCATGACATTATTGTTGTGGCCTTTAGAGGCACTGAACCCTTTGATGCGGATGCATGGTGCACTGACTTTGACATCTCCTGGTATCAACTTGAAGGGGTTGGAAAGATCCATGGAGGTTTCATGAAAGCTCTAGGTTTACAGAGAGATGTTGGTTGGCCGGAGCCAGAGAATATCAAACAAGTCGATGCCGGCCCAGGGGGCTTAGCTTACTATGCCATCAGGGACATGCTGAAAAAGCTTCTGCTTGAAAATGAGAGAGCAAAATTTATAGTAACTGGGCACAGTTTAGGTGGTGCACTAGCAATTCTCTTTCCAGCAGTTCTAACATTTCACAAGGGAAATGATTCAAAACTCTTGTTGGAGAGGTTGGAGGGAGTTTATACATTCGGGCAACCTAGGGTTGGTGATAAGGTTTTTGGGGAGTACATGGAAAAGAAAATGATACAGCACAAAATTAATTATTTTAGGTTTGTTTATGGGAATGACCTGGTGCCTAGGTTGCCTTATGATGACAAGGCCCTAATGTTCAAGCACTTTGGGAATTGCATCTACAGTAATAGGGAATATGAACTGCAGGTTCTGCCGGAAGAGCCAAACAAGAATTACTTCTCCCCAGAGAAGGTAATACCGATGGTGGCCAATGTTTTTGGTGAGCTGAAAAGAAGTTTCACCATTCCGTATATGAAGGGACCTGACTACAAAGAAGGGCGATTCCTCAAAATGGTTAGGCTAATGGGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

373

Amino Acids

42.71

Weight (kDa)

8.42

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 130 - 295 1e-32 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 940
AccB1I GGYRCC 1 cut(s) 865
AccIII TCCGGA 1 cut(s) 312
AciI CCGC 2 cut(s) 371, 422
AclI AACGTT 1 cut(s) 125
AclWI GGATC 2 cut(s) 29, 475
AcoI YGGCCR 2 cut(s) 530, 1011
AcsI RAATTY 4 cut(s) 79, 145, 248, 638
AfaI GTAC 4 cut(s) 52, 105, 239, 800
AfiI CCNNNNNNNGG 4 cut(s) 570, 776, 994, 1096
AgsI TTSAA 6 cut(s) 37, 76, 467, 623, 723, 904
AjnI CCWGG 3 cut(s) 452, 568, 861
AjuI GAANNNNNNNTTGG 2 cut(s) 336, 368
AluBI AGCT 5 cut(s) 166, 503, 581, 613, 1030
AluI AGCT 5 cut(s) 166, 503, 581, 613, 1030
Alw21I GWGCWC 2 cut(s) 437, 673
Alw44I GTGCAC 2 cut(s) 433, 669
AlwI GGATC 2 cut(s) 29, 475
AlwNI CAGNNNCTG 1 cut(s) 955
Aor13HI TCCGGA 1 cut(s) 312
AoxI GGCC 5 cut(s) 393, 530, 565, 891, 1011
ApaLI GTGCAC 2 cut(s) 433, 669
ApeKI GCWGC 2 cut(s) 85, 206
ApoI RAATTY 4 cut(s) 79, 145, 248, 638
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 4 cut(s) 41, 248, 718, 980
AspA2I CCTAGG 2 cut(s) 770, 869
AspS9I GGNCC 4 cut(s) 373, 566, 892, 1064
AsuHPI GGTGA 4 cut(s) 104, 791, 1036, 1037
AvaII GGWCC 2 cut(s) 373, 1064
AvrII CCTAGG 2 cut(s) 770, 869
BaeGI GKGCMC 3 cut(s) 437, 657, 673
BalI TGGCCA 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 865
BarI GAAGNNNNNNTAC 2 cut(s) 208, 240
Bbv12I GWGCWC 2 cut(s) 437, 673
BbvI GCAGC 2 cut(s) 72, 218
BccI CCATC 4 cut(s) 214, 280, 599, 1000
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 3 cut(s) 454, 570, 863
BfaI CTAG 4 cut(s) 506, 674, 771, 870
BfmI CTRYAG 3 cut(s) 264, 925, 947
BfuAI ACCTGC 1 cut(s) 940
BglI GCCNNNNNGGC 1 cut(s) 573
BglII AGATCT 1 cut(s) 27
BisI GCNGC 2 cut(s) 86, 207
BlnI CCTAGG 2 cut(s) 770, 869
BlpI GCTNAGC 1 cut(s) 577
BlsI GCNGC 2 cut(s) 87, 208
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 3 cut(s) 454, 570, 863
Bme18I GGWCC 2 cut(s) 373, 1064
BmgT120I GGNCC 4 cut(s) 373, 566, 892, 1064
BmiI GGNNCC 3 cut(s) 537, 867, 1065
BmrFI CCNGG 3 cut(s) 454, 570, 863
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 5 cut(s) 186, 409, 415, 550, 930
BmuI ACTGGG 1 cut(s) 660
Bpu1102I GCTNAGC 1 cut(s) 577
BpuEI CTTGAG 2 cut(s) 31, 103
BsaAI YACGTR 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 5 cut(s) 484, 568, 569, 770, 869
BsaWI WCCGGW 1 cut(s) 312
Bsc4I CCNNNNNNNGG 4 cut(s) 570, 776, 994, 1096
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 2 cut(s) 284, 655
Bse8I GATNNNNATC 1 cut(s) 26
BseAI TCCGGA 1 cut(s) 312
BseBI CCWGG 3 cut(s) 454, 570, 863
BseDI CCNNGG 5 cut(s) 484, 568, 569, 770, 869
BseGI GGATG 1 cut(s) 430
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 4 cut(s) 570, 776, 994, 1096
BseMII CTCAG 1 cut(s) 226
BseNI ACTGG 2 cut(s) 284, 655
BseSI GKGCMC 3 cut(s) 437, 657, 673
BseXI GCAGC 2 cut(s) 72, 218
BshFI GGCC 5 cut(s) 395, 532, 567, 893, 1013
BshNI GGYRCC 1 cut(s) 865
BsiHKAI GWGCWC 2 cut(s) 437, 673
BsiSI CCGG 4 cut(s) 313, 533, 564, 959
BsiWI CGTACG 1 cut(s) 237
BslFI GGGAC 2 cut(s) 611, 1077
BslI CCNNNNNNNGG 4 cut(s) 570, 776, 994, 1096
BsmFI GGGAC 2 cut(s) 611, 1077
BsnI GGCC 5 cut(s) 395, 532, 567, 893, 1013
Bsp1286I GDGCHC 3 cut(s) 437, 657, 673
Bsp13I TCCGGA 1 cut(s) 312
Bsp143I GATC 4 cut(s) 21, 27, 274, 480
Bsp1720I GCTNAGC 1 cut(s) 577
Bsp19I CCATGG 1 cut(s) 484
BspACI CCGC 2 cut(s) 371, 422
BspANI GGCC 5 cut(s) 395, 532, 567, 893, 1013
BspCNI CTCAG 1 cut(s) 225
BspEI TCCGGA 1 cut(s) 312
BspHI TCATGA 1 cut(s) 495
BspLI GGNNCC 3 cut(s) 537, 867, 1065
BspMAI CTGCAG 1 cut(s) 951
BspMI ACCTGC 1 cut(s) 940
BspPI GGATC 2 cut(s) 29, 475
BspQI GCTCTTC 1 cut(s) 957
BspT107I GGYRCC 1 cut(s) 865
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 2 cut(s) 284, 655
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 5 cut(s) 484, 568, 569, 770, 869
BssMI GATC 4 cut(s) 21, 27, 274, 480
BssT1I CCWWGG 3 cut(s) 484, 770, 869
Bst2UI CCWGG 3 cut(s) 454, 570, 863
Bst4CI ACNGT 4 cut(s) 72, 268, 659, 929
Bst6I CTCTTC 1 cut(s) 957
BstAPI GCANNNNNTGC 1 cut(s) 955
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 565
BstDEI CTNAG 2 cut(s) 212, 577
BstDSI CCRYGG 1 cut(s) 484
BstF5I GGATG 1 cut(s) 430
BstKTI GATC 4 cut(s) 24, 30, 277, 483
BstMBI GATC 4 cut(s) 21, 27, 274, 480
BstMWI GCNNNNNNNGC 6 cut(s) 573, 587, 610, 874, 955, 964
BstNI CCWGG 3 cut(s) 454, 570, 863
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 3 cut(s) 452, 568, 861
BstSFI CTRYAG 3 cut(s) 264, 925, 947
BstSLI GKGCMC 3 cut(s) 437, 657, 673
BstV1I GCAGC 2 cut(s) 72, 218
BstX2I RGATCY 2 cut(s) 27, 480
BstYI RGATCY 2 cut(s) 27, 480
BsuRI GGCC 5 cut(s) 395, 532, 567, 893, 1013
BtgI CCRYGG 1 cut(s) 484
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 430
BtsIMutI CAGTG 3 cut(s) 277, 405, 435
BveI ACCTGC 1 cut(s) 940
Cac8I GCNNGC 1 cut(s) 565
CaiI CAGNNNCTG 1 cut(s) 955
CciI TCATGA 1 cut(s) 495
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 4 cut(s) 373, 566, 892, 1064
CsiI ACCWGGT 1 cut(s) 861
Csp6I GTAC 4 cut(s) 51, 104, 238, 799
CviAII CATG 7 cut(s) 185, 377, 429, 485, 496, 601, 802
CviQI GTAC 4 cut(s) 51, 104, 238, 799
DdeI CTNAG 2 cut(s) 212, 577
DpnI GATC 4 cut(s) 23, 29, 276, 482
DpnII GATC 4 cut(s) 21, 27, 274, 480
EaeI YGGCCR 2 cut(s) 530, 1011
Eam1104I CTCTTC 1 cut(s) 957
EarI CTCTTC 1 cut(s) 957
Eco130I CCWWGG 3 cut(s) 484, 770, 869
Eco47I GGWCC 2 cut(s) 373, 1064
EcoO109I RGGNCCY 2 cut(s) 892, 1064
EcoRII CCWGG 3 cut(s) 452, 568, 861
EcoT14I CCWWGG 3 cut(s) 484, 770, 869
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 3 cut(s) 484, 770, 869
FaeI CATG 7 cut(s) 188, 380, 432, 488, 499, 604, 805
FalI AAGNNNNNCTT 2 cut(s) 965, 997
FaqI GGGAC 2 cut(s) 611, 1077
FatI CATG 7 cut(s) 184, 376, 428, 484, 495, 600, 801
Fnu4HI GCNGC 2 cut(s) 86, 207
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 2 cut(s) 86, 207
FspBI CTAG 4 cut(s) 506, 674, 771, 870
GluI GCNGC 2 cut(s) 86, 207
HaeIII GGCC 5 cut(s) 395, 532, 567, 893, 1013
HapII CCGG 4 cut(s) 313, 533, 564, 959
Hin1II CATG 7 cut(s) 188, 380, 432, 488, 499, 604, 805
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 3 cut(s) 116, 719, 1086
HpaII CCGG 4 cut(s) 313, 533, 564, 959
HphI GGTGA 4 cut(s) 104, 791, 1036, 1037
Hpy166II GTNNAC 3 cut(s) 435, 512, 671
Hpy188I TCNGA 1 cut(s) 68
Hpy188III TCNNGA 4 cut(s) 25, 120, 313, 496
Hpy8I GTNNAC 3 cut(s) 435, 512, 671
HpyAV CCTTC 4 cut(s) 461, 988, 1054, 1073
HpyCH4III ACNGT 4 cut(s) 72, 268, 659, 929
HpyCH4IV ACGT 2 cut(s) 125, 358
HpyCH4V TGCA 6 cut(s) 206, 428, 435, 671, 921, 949
HpyF10VI GCNNNNNNNGC 6 cut(s) 573, 587, 610, 874, 955, 964
HpyF3I CTNAG 2 cut(s) 212, 577
HpySE526I ACGT 2 cut(s) 125, 358
Hsp92II CATG 7 cut(s) 188, 380, 432, 488, 499, 604, 805
Kpn2I TCCGGA 1 cut(s) 312
KroI GCCGGC 1 cut(s) 563
KroNI GCCGGC 1 cut(s) 565
Kzo9I GATC 4 cut(s) 21, 27, 274, 480
LguI GCTCTTC 1 cut(s) 957
LmnI GCTCC 2 cut(s) 163, 535
Lsp1109I GCAGC 2 cut(s) 72, 218
LweI GCATC 5 cut(s) 186, 409, 415, 550, 930
MabI ACCWGGT 1 cut(s) 861
MaeI CTAG 4 cut(s) 506, 674, 771, 870
MaeII ACGT 2 cut(s) 125, 358
MaeIII GTNAC 2 cut(s) 359, 646
MalI GATC 4 cut(s) 23, 29, 276, 482
MboI GATC 4 cut(s) 21, 27, 274, 480
MboII GAAGA 2 cut(s) 295, 974
MflI RGATCY 2 cut(s) 27, 480
MhlI GDGCHC 3 cut(s) 437, 657, 673
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 9 cut(s) 79, 145, 248, 638, 678, 828, 832, 916, 976
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 4 cut(s) 46, 454, 714, 723
MnlI CCTC 7 cut(s) 83, 88, 395, 482, 732, 739, 1100
Mox20I TGGCCA 1 cut(s) 1013
Mph1103I ATGCAT 1 cut(s) 430
MroI TCCGGA 1 cut(s) 312
MroNI GCCGGC 1 cut(s) 563
MroXI GAANNNNTTC 4 cut(s) 41, 248, 718, 980
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 1 cut(s) 831
MslI CAYNNNNRTG 1 cut(s) 189
Msp20I TGGCCA 1 cut(s) 1013
MspI CCGG 4 cut(s) 313, 533, 564, 959
MspR9I CCNGG 3 cut(s) 454, 570, 863
MvaI CCWGG 3 cut(s) 454, 570, 863
MwoI GCNNNNNNNGC 6 cut(s) 573, 587, 610, 874, 955, 964
NaeI GCCGGC 1 cut(s) 565
NcoI CCATGG 1 cut(s) 484
NdeII GATC 4 cut(s) 21, 27, 274, 480
NgoMIV GCCGGC 1 cut(s) 563
NlaIII CATG 7 cut(s) 188, 380, 432, 488, 499, 604, 805
NlaIV GGNNCC 3 cut(s) 537, 867, 1065
NmuCI GTSAC 1 cut(s) 359
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 604
PagI TCATGA 1 cut(s) 495
PasI CCCWGGG 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 957
PcsI WCGNNNNNNNCGW 2 cut(s) 131, 303
PdiI GCCGGC 1 cut(s) 565
PdmI GAANNNNTTC 4 cut(s) 41, 248, 718, 980
PfeI GAWTC 2 cut(s) 719, 1086
Pfl23II CGTACG 1 cut(s) 237
PkrI GCNGC 2 cut(s) 87, 208
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 1 cut(s) 1064
PshBI ATTAAT 1 cut(s) 831
Psp1406I AACGTT 1 cut(s) 125
Psp5II RGGWCCY 1 cut(s) 1064
Psp6I CCWGG 3 cut(s) 452, 568, 861
PspGI CCWGG 3 cut(s) 452, 568, 861
PspLI CGTACG 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 537, 867, 1065
PspPI GGNCC 4 cut(s) 373, 566, 892, 1064
PspPPI RGGWCCY 1 cut(s) 1064
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PstI CTGCAG 1 cut(s) 951
PstNI CAGNNNCTG 1 cut(s) 955
PsuI RGATCY 2 cut(s) 27, 480
RsaI GTAC 4 cut(s) 52, 105, 239, 800
RsaNI GTAC 4 cut(s) 51, 104, 238, 799
RseI CAYNNNNRTG 1 cut(s) 189
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 1 cut(s) 831
SatI GCNGC 2 cut(s) 86, 207
Sau3AI GATC 4 cut(s) 21, 27, 274, 480
Sau96I GGNCC 4 cut(s) 373, 566, 892, 1064
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 3 cut(s) 454, 570, 863
SduI GDGCHC 3 cut(s) 437, 657, 673
SexAI ACCWGGT 1 cut(s) 861
SfaNI GCATC 5 cut(s) 186, 409, 415, 550, 930
SfcI CTRYAG 3 cut(s) 264, 925, 947
SinI GGWCC 2 cut(s) 373, 1064
SmiMI CAYNNNNRTG 1 cut(s) 189
SmlI CTYRAG 2 cut(s) 46, 118
SmoI CTYRAG 2 cut(s) 46, 118
Sse9I AATT 9 cut(s) 79, 145, 248, 638, 678, 828, 832, 916, 976
SsiI CCGC 2 cut(s) 371, 422
SspMI CTAG 4 cut(s) 506, 674, 771, 870
StyD4I CCNGG 3 cut(s) 452, 568, 861
StyI CCWWGG 3 cut(s) 484, 770, 869
TaaI ACNGT 4 cut(s) 72, 268, 659, 929
TaiI ACGT 2 cut(s) 128, 361
TaqI TCGA 3 cut(s) 24, 258, 558
TasI AATT 9 cut(s) 79, 145, 248, 638, 678, 828, 832, 916, 976
TatI WGTACW 2 cut(s) 50, 798
TfiI GAWTC 2 cut(s) 719, 1086
Tru1I TTAA 1 cut(s) 831
Tru9I TTAA 1 cut(s) 831
TscAI CASTG 3 cut(s) 284, 412, 442
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 2 cut(s) 85, 206
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 6 cut(s) 127, 283, 484, 512, 957, 1073
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 3 cut(s) 284, 412, 442
VneI GTGCAC 2 cut(s) 433, 669
VpaK11BI GGWCC 2 cut(s) 373, 1064
VspI ATTAAT 1 cut(s) 831
XapI RAATTY 4 cut(s) 79, 145, 248, 638
XceI RCATGY 1 cut(s) 604
XmaJI CCTAGG 2 cut(s) 770, 869
XmnI GAANNNNTTC 4 cut(s) 41, 248, 718, 980
XspI CTAG 4 cut(s) 506, 674, 771, 870
ZrmI AGTACT 1 cut(s) 52
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.