RLG00000017456

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003099
Physical Location & Seq
Reverse (-)
17041578 .. 17045376
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017456

Sequence Viewer

Length: 1449 bp
ATGGCCTGCGACAAGGGATTTTCCAGCAGTTTTATGCTGTTGAAGCCAGAAGAAGTCGGATTGTTTGGTCTGTTTCACATTTTGTTCTCCAGCAAGTTAGAGGAAAAGAAATTTGTAGATTGCTCGGAGGGGACTGTCACTGAGGAGATCAGCTTCAGGCGCAGATGGCTCATATTTGTTTCCATCTTGGCTCAAAAGTTTCTGCAACTTGTGTCAAAGCCACTCTCAGTTGTCGGATCTGGGATCGAAATGTGTTTGAACCTTGTATCCGTCAACCGCAATGTTTTTGTGCTCGTGATCAATTTCTTCCGAGGGGTGGTGGTAAAGCCGGATAAGACATCAGCAACATTCCTGTCATTTATAGGAAATTTTGACAAGAGGGTGGAGTTGGATAGCACCATTAAATATGATGATGCCAAGTACCATGCAGCTCTCTCTATGATGGCTTCTAAAGCATCCTATGAGAATAATGCCTACATTGAAACTACTGTTAGGGATCATTGGAAGATGGAGTTCTTCGGATCTTTCGACTTCTGGAATGATTATCAACAAAAAGCCACAACACAAGCCTTTATGCTTCGGGATAAGTCTTCTGACCATGACACAGTTGTTGTGGCCTTTAGGGGAACCGAACCGTTTGATGCAGATGCATGGTGCTCCGACTTTGACATCTCTTGGTATGAGCTTTCAGGGGTCGGAAAAATTCATGGCGGCTTTATGAAAGCTCTAGGTTTACAAAAGAATGTTGGTTGGCCTAAGGAAACCAAACAAGAAGATGGTCATCCAAACTTGGCTTACTATGCAATTAGGGACAGGCTAAGCAAGCTTTTGAGCCAAAATGATCAAGCTAGATACATATTGACAGGGCACAGTTTGGGAGGGGCTTTAGCAATTTTGTTTCCAGCAGTTTTGGCCCTCCACAATGATACATGGTTATTGGAGAGATTGCAAGGGGTATATACATTTGGGCAACCAAGGGTAGGAGATGAAAAGTTTGGTGAGTTCATGGAGAAACAGTTGCAAGAGCATAACTTTCAGTATTTTAGATTTGTTTATGGTAATGATATGGTTCCTCGGTTGCCTTATGATGACAAAGCCTTGATGTTCAAGCACTTTGGGACATGCCTCTATTATAATAGGCACTATGAAGTGAAGGTTGTGGACCAGGAACCTAATAAGAACTACTTCTCCCCATTGGGGGCAATACCAATGATAATAAATGCATTTTTCGAGCTAATTAGAAGCTTCACCATTGCAAAAACAAAGGGGCCAGATTATAAAGAAGGGATTTTTCTCAGAATTTTCAGGATAATTGGCCTGGTATTTCCTGGGATTCCAGCTCATAGCACTCAAGATTATGTGAATGCGACCAGGTTGGGGTCCTCGGACGTCTTCCTCCTACCAAAAAACACTTCATCCCATGAAGATGTATCAAGCTTACAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

483

Amino Acids

55.1

Weight (kDa)

6.51

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 204 - 363 7.5e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1134, 1278
AatII GACGTC 1 cut(s) 1392
AciI CCGC 2 cut(s) 277, 711
AclWI GGATC 4 cut(s) 244, 251, 504, 529
AcsI RAATTY 4 cut(s) 110, 367, 702, 1299
AcuI CTGAAG 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 1389
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 5 cut(s) 316, 980, 1197, 1198, 1377
AgsI TTSAA 4 cut(s) 43, 259, 482, 1108
AjnI CCWGG 4 cut(s) 1164, 1317, 1327, 1370
AjuI GAANNNNNNNTTGG 2 cut(s) 1396, 1428
AloI GAACNNNNNNTCC 4 cut(s) 251, 283, 1172, 1204
Alw21I GWGCWC 2 cut(s) 294, 659
AlwI GGATC 4 cut(s) 244, 251, 504, 529
AoxI GGCC 6 cut(s) 3, 615, 752, 912, 1268, 1315
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 4 cut(s) 110, 367, 702, 1299
Asp700I GAANNNNTTC 1 cut(s) 1184
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 4 cut(s) 913, 1162, 1268, 1380
AsuHPI GGTGA 2 cut(s) 1010, 1240
AvaII GGWCC 2 cut(s) 1162, 1380
AxyI CCTNAGG 1 cut(s) 756
BaeGI GKGCMC 1 cut(s) 870
BauI CACGAG 1 cut(s) 293
BbsI GAAGAC 2 cut(s) 582, 1384
Bbv12I GWGCWC 2 cut(s) 294, 659
BbvI GCAGC 1 cut(s) 440
BccI CCATC 5 cut(s) 159, 191, 436, 502, 770
BciT130I CCWGG 4 cut(s) 1166, 1319, 1329, 1372
BciVI GTATCC 1 cut(s) 277
BclI TGATCA 2 cut(s) 297, 841
BfaI CTAG 2 cut(s) 728, 849
BfuI GTATCC 1 cut(s) 277
BisI GCNGC 2 cut(s) 429, 712
BlpI GCTNAGC 1 cut(s) 818
BlsI GCNGC 2 cut(s) 430, 713
Bme1390I CCNGG 4 cut(s) 1166, 1319, 1329, 1372
Bme18I GGWCC 2 cut(s) 1162, 1380
BmgT120I GGNCC 4 cut(s) 913, 1162, 1268, 1380
BmiI GGNNCC 5 cut(s) 628, 1071, 1170, 1269, 1381
BmrFI CCNGG 4 cut(s) 1166, 1319, 1329, 1372
BmsI GCATC 4 cut(s) 403, 464, 631, 637
BpiI GAAGAC 2 cut(s) 582, 1384
BpmI CTGGAG 1 cut(s) 73
Bpu1102I GCTNAGC 1 cut(s) 818
BpuEI CTTGAG 1 cut(s) 1335
BsaBI GATNNNNATC 1 cut(s) 780
BsaHI GRCGYC 1 cut(s) 1389
BsaJI CCNNGG 5 cut(s) 310, 974, 1073, 1328, 1383
BsaXI ACNNNNNCTCC 4 cut(s) 1001, 1031, 1172, 1202
Bsc4I CCNNNNNNNGG 5 cut(s) 316, 980, 1197, 1198, 1377
Bse21I CCTNAGG 1 cut(s) 756
Bse3DI GCAATG 2 cut(s) 286, 1251
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 4 cut(s) 1166, 1319, 1329, 1372
BseDI CCNNGG 5 cut(s) 310, 974, 1073, 1328, 1383
BseGI GGATG 3 cut(s) 455, 781, 1415
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 5 cut(s) 316, 980, 1197, 1198, 1377
BseMI GCAATG 2 cut(s) 286, 1251
BseMII CTCAG 3 cut(s) 132, 240, 1309
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 870
BseXI GCAGC 1 cut(s) 440
BshFI GGCC 6 cut(s) 5, 617, 754, 914, 1270, 1317
BsiHKAI GWGCWC 2 cut(s) 294, 659
BsiSI CCGG 1 cut(s) 329
BslFI GGGAC 3 cut(s) 145, 824, 1132
BslI CCNNNNNNNGG 5 cut(s) 316, 980, 1197, 1198, 1377
BsmFI GGGAC 3 cut(s) 145, 824, 1132
BsmI GAATGC 1 cut(s) 1369
BsnI GGCC 6 cut(s) 5, 617, 754, 914, 1270, 1317
Bsp1286I GDGCHC 3 cut(s) 294, 659, 870
Bsp143I GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
Bsp1720I GCTNAGC 1 cut(s) 818
BspACI CCGC 2 cut(s) 277, 711
BspANI GGCC 6 cut(s) 5, 617, 754, 914, 1270, 1317
BspCNI CTCAG 3 cut(s) 133, 239, 1308
BspLI GGNNCC 5 cut(s) 628, 1071, 1170, 1269, 1381
BspPI GGATC 4 cut(s) 244, 251, 504, 529
BsrDI GCAATG 2 cut(s) 286, 1251
BssECI CCNNGG 5 cut(s) 310, 974, 1073, 1328, 1383
BssMI GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
BssNI GRCGYC 1 cut(s) 1389
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 974
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 4 cut(s) 1166, 1319, 1329, 1372
Bst4CI ACNGT 6 cut(s) 136, 490, 607, 636, 872, 1017
BstACI GRCGYC 1 cut(s) 1389
BstC8I GCNNGC 2 cut(s) 7, 824
BstDEI CTNAG 5 cut(s) 141, 226, 756, 818, 1295
BstF5I GGATG 3 cut(s) 455, 781, 1415
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 7 cut(s) 150, 239, 246, 300, 499, 524, 844
BstMBI GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
BstMWI GCNNNNNNNGC 7 cut(s) 43, 159, 166, 452, 800, 823, 911
BstNI CCWGG 4 cut(s) 1166, 1319, 1329, 1372
BstNSI RCATGY 1 cut(s) 1125
BstSCI CCNGG 4 cut(s) 1164, 1317, 1327, 1370
BstSLI GKGCMC 1 cut(s) 870
BstV1I GCAGC 1 cut(s) 440
BstV2I GAAGAC 2 cut(s) 582, 1384
BstX2I RGATCY 2 cut(s) 236, 521
BstYI RGATCY 2 cut(s) 236, 521
Bsu36I CCTNAGG 1 cut(s) 756
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 6 cut(s) 5, 617, 754, 914, 1270, 1317
BtsCI GGATG 3 cut(s) 455, 781, 1415
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 7, 824
CfoI GCGC 1 cut(s) 162
Cfr13I GGNCC 4 cut(s) 913, 1162, 1268, 1380
CsiI ACCWGGT 1 cut(s) 1370
Csp6I GTAC 1 cut(s) 421
CviAII CATG 8 cut(s) 425, 599, 651, 707, 930, 1006, 1122, 1421
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 5 cut(s) 141, 226, 756, 818, 1295
DpnI GATC 7 cut(s) 149, 238, 245, 299, 498, 523, 843
DpnII GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
Eco130I CCWWGG 1 cut(s) 974
Eco47I GGWCC 2 cut(s) 1162, 1380
Eco57I CTGAAG 1 cut(s) 139
Eco81I CCTNAGG 1 cut(s) 756
EcoO109I RGGNCCY 1 cut(s) 1380
EcoRII CCWGG 4 cut(s) 1164, 1317, 1327, 1370
EcoT14I CCWWGG 1 cut(s) 974
EcoT22I ATGCAT 2 cut(s) 652, 1225
ErhI CCWWGG 1 cut(s) 974
FaeI CATG 8 cut(s) 428, 602, 654, 710, 933, 1009, 1125, 1424
FalI AAGNNNNNCTT 2 cut(s) 1169, 1201
FaqI GGGAC 3 cut(s) 145, 824, 1132
FatI CATG 8 cut(s) 424, 598, 650, 706, 929, 1005, 1121, 1420
FbaI TGATCA 2 cut(s) 297, 841
Fnu4HI GCNGC 2 cut(s) 429, 712
FokI GGATG 3 cut(s) 442, 768, 1402
Fsp4HI GCNGC 2 cut(s) 429, 712
FspBI CTAG 2 cut(s) 728, 849
GlaI GCGC 1 cut(s) 161
GluI GCNGC 2 cut(s) 429, 712
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 6 cut(s) 5, 617, 754, 914, 1270, 1317
HapII CCGG 1 cut(s) 329
HhaI GCGC 1 cut(s) 162
Hin1I GRCGYC 1 cut(s) 1389
Hin1II CATG 8 cut(s) 428, 602, 654, 710, 933, 1009, 1125, 1424
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 3 cut(s) 824, 1243, 1435
HinfI GANTC 1 cut(s) 1333
HpaII CCGG 1 cut(s) 329
HphI GGTGA 2 cut(s) 1010, 1240
Hpy166II GTNNAC 3 cut(s) 274, 734, 1162
Hpy188III TCNNGA 5 cut(s) 295, 535, 581, 1306, 1352
Hpy8I GTNNAC 3 cut(s) 274, 734, 1162
HpyAV CCTTC 2 cut(s) 1147, 1277
HpyCH4III ACNGT 6 cut(s) 136, 490, 607, 636, 872, 1017
HpyCH4IV ACGT 1 cut(s) 1389
HpyCH4V TGCA 9 cut(s) 205, 428, 644, 650, 803, 949, 1021, 1223, 1256
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 159, 166, 452, 800, 823, 911
HpyF3I CTNAG 5 cut(s) 141, 226, 756, 818, 1295
HpySE526I ACGT 1 cut(s) 1389
Hsp92I GRCGYC 1 cut(s) 1389
Hsp92II CATG 8 cut(s) 428, 602, 654, 710, 933, 1009, 1125, 1424
HspAI GCGC 1 cut(s) 160
Ksp22I TGATCA 2 cut(s) 297, 841
Kzo9I GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
LmnI GCTCC 1 cut(s) 662
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 4 cut(s) 403, 464, 631, 637
MabI ACCWGGT 1 cut(s) 1370
MaeI CTAG 2 cut(s) 728, 849
MaeII ACGT 1 cut(s) 1389
MaeIII GTNAC 1 cut(s) 136
MalI GATC 7 cut(s) 149, 238, 245, 299, 498, 523, 843
MboI GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
MboII GAAGA 8 cut(s) 62, 298, 508, 517, 582, 785, 1384, 1436
MflI RGATCY 2 cut(s) 236, 521
MhlI GDGCHC 3 cut(s) 294, 659, 870
MluCI AATT 9 cut(s) 110, 301, 367, 702, 804, 891, 1236, 1299, 1311
MmeI TCCRAC 5 cut(s) 37, 214, 369, 676, 684
Mph1103I ATGCAT 2 cut(s) 652, 1225
MroXI GAANNNNTTC 1 cut(s) 1184
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 1425
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 4 cut(s) 1166, 1319, 1329, 1372
Mva1269I GAATGC 1 cut(s) 1369
MvaI CCWGG 4 cut(s) 1166, 1319, 1329, 1372
MwoI GCNNNNNNNGC 7 cut(s) 43, 159, 166, 452, 800, 823, 911
NdeII GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
NlaIII CATG 8 cut(s) 428, 602, 654, 710, 933, 1009, 1125, 1424
NlaIV GGNNCC 5 cut(s) 628, 1071, 1170, 1269, 1381
NmuCI GTSAC 1 cut(s) 136
NsiI ATGCAT 2 cut(s) 652, 1225
NspI RCATGY 1 cut(s) 1125
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PctI GAATGC 1 cut(s) 1369
PdmI GAANNNNTTC 1 cut(s) 1184
PfeI GAWTC 1 cut(s) 1333
PkrI GCNGC 2 cut(s) 430, 713
PpuMI RGGWCCY 1 cut(s) 1380
PsiI TTATAA 2 cut(s) 1134, 1278
Psp5II RGGWCCY 1 cut(s) 1380
Psp6I CCWGG 4 cut(s) 1164, 1317, 1327, 1370
PspGI CCWGG 4 cut(s) 1164, 1317, 1327, 1370
PspN4I GGNNCC 5 cut(s) 628, 1071, 1170, 1269, 1381
PspPI GGNCC 4 cut(s) 913, 1162, 1268, 1380
PspPPI RGGWCCY 1 cut(s) 1380
PsuI RGATCY 2 cut(s) 236, 521
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
RseI CAYNNNNRTG 1 cut(s) 1425
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 2 cut(s) 429, 712
Sau3AI GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
Sau96I GGNCC 4 cut(s) 913, 1162, 1268, 1380
ScrFI CCNGG 4 cut(s) 1166, 1319, 1329, 1372
SduI GDGCHC 3 cut(s) 294, 659, 870
SexAI ACCWGGT 1 cut(s) 1370
SfaNI GCATC 4 cut(s) 403, 464, 631, 637
SinI GGWCC 2 cut(s) 1162, 1380
SmiMI CAYNNNNRTG 1 cut(s) 1425
SmlI CTYRAG 1 cut(s) 1350
SmoI CTYRAG 1 cut(s) 1350
Sse9I AATT 9 cut(s) 110, 301, 367, 702, 804, 891, 1236, 1299, 1311
SsiI CCGC 2 cut(s) 277, 711
SspMI CTAG 2 cut(s) 728, 849
StyD4I CCNGG 4 cut(s) 1164, 1317, 1327, 1370
StyI CCWWGG 1 cut(s) 974
TaaI ACNGT 6 cut(s) 136, 490, 607, 636, 872, 1017
TaiI ACGT 1 cut(s) 1392
TaqI TCGA 3 cut(s) 246, 528, 1230
TasI AATT 9 cut(s) 110, 301, 367, 702, 804, 891, 1236, 1299, 1311
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 1 cut(s) 1333
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 7 cut(s) 695, 734, 994, 1002, 1161, 1404, 1437
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 2 cut(s) 1162, 1380
XapI RAATTY 4 cut(s) 110, 367, 702, 1299
XceI RCATGY 1 cut(s) 1125
XmnI GAANNNNTTC 1 cut(s) 1184
XspI CTAG 2 cut(s) 728, 849
ZraI GACGTC 1 cut(s) 1390
Zsp2I ATGCAT 2 cut(s) 652, 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.