MD15G1284800.v1.1

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003099
Physical Location & Seq
Reverse (-)
25889690 .. 25892943
3254 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1284800.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGCTTCTTGTAACAAGAGTTTCTCTTGCAAATACATGTTGTTGGATCCCAACGATGTGGGATTCTTTGATCTGATTCACATCTTGTTTTCCAGTAATTTGAAGAGGAGGAGGTTTGTCGACTCTTCTATGGATACCGAGGACGACCCTGACCGGAGGTGGCTCATATTTCTCTCCATTGTGGTGCAAAAGTTGCTTCAGTTTGTGGCAACGCCAATGGCAATGATTGGCAGGGGGATCGAGCTCTGCTTGAACCTTTTCTCAAATACTCAAAACTTGGTCGGGCTGCTCCAGAATTTGCTGCGAGGTGAGTATATAATACCAAAGAAAGACTCAAGAACGTTCGTGTCGTTCATTGGAAATTTGGACAAGCGAGTAGAGCTGGACAAAAGCATCAAACATGGAGATGGTAGGTATAATGCAGCACTTAGTATGATGGCGTCTAAAATATCGTATGAGAACAAATTCTACATCGAAACTACAGTCCTAGATCACTGGAAGATGGAGTTCGTGGGCTCTTACGACTTCCGGAATGATTATCAAGGAAAATCGACAACACAGGCATGTATTCTACGTGACAGAAGTGCCGACCGTGACATTATTGTTGTAGCCTTTAGAGGCACTGAACCCTTCGATGCAGATGCATGGTGCACTGACCTTGACATCTCCTGGTATAAACTAAAAGGGGTCGGAAAGATCCATGGAGGTTTCATGAAAGCTCTAGGTTTACAGAGAGATGTTGGTTGGCCGGAGCCGGAGAAAATCAAACAAGTGGATAAGAACCCAGGCGGCTTAGCTTACTATGCCGTTAGGGACATGCTGAAAATACTTCTGCTTGAAAATGACAAAGCAAAATTTATAGTAACTGGCCACAGTTTAGGTGGTGCGCTAGCAATTCTCCTTCCAGCAGTTTTAACATTTCACAAGGGAAATGATTCAGAACTCCTGTTGGAGAGGTTGGAGGGAGTTTATACATTTGGGCAACCTAGGGTTGGTGACAAGGTTTTCGGGGAGTACATGGAAAAGAAAATGAAAGAGCATAAGATTAATTATTTTAGGTTCGTTTATGGGAATGACTTGGTGCCGAGGCTGCCTTACGATGACAAGGCCCTAATGTTCAAGCACTTTGGGAAGTGCCTCTACTATAATAGGGAATATGAAGTGCAGGTTCTTCCAGAAGAGCCAAATAAGAATTACTTCTCCCCAGAGAAAATAATACCGATGATGGCGAATGCTTTTGGTGAGCTGGTAAGAAGTTTCACCATTCCAGACAAGAAGGGACTGGACTACAAAGAAGGGTGCTTCTTTAAGATGATTAGGTTAATGGGTTTGGTAATGCCAGGAGCAGCAGCACATTGTCCCCAAGATTATGTAAACGCCACCAGATTGGGATCCTCGGATGTGTTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

471

Amino Acids

53.82

Weight (kDa)

8.4

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 202 - 367 6.9e-32 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1156
AccB1I GGYRCC 1 cut(s) 1081
AccI GTMKAC 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 528
AciI CCGC 1 cut(s) 789
AclI AACGTT 1 cut(s) 341
AclWI GGATC 6 cut(s) 41, 54, 245, 691, 1386, 1399
AcoI YGGCCR 2 cut(s) 746, 868
AcsI RAATTY 4 cut(s) 295, 361, 464, 854
AcuI CTGAAG 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 440
AfaI GTAC 1 cut(s) 1016
AfiI CCNNNNNNNGG 1 cut(s) 992
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 4 cut(s) 103, 253, 839, 1120
AjnI CCWGG 3 cut(s) 668, 784, 1339
AluBI AGCT 5 cut(s) 244, 382, 719, 797, 1246
AluI AGCT 5 cut(s) 244, 382, 719, 797, 1246
Alw21I GWGCWC 2 cut(s) 246, 653
Alw44I GTGCAC 1 cut(s) 649
AlwI GGATC 6 cut(s) 41, 54, 245, 691, 1386, 1399
Aor13HI TCCGGA 1 cut(s) 528
AoxI GGCC 3 cut(s) 746, 868, 1107
ApaLI GTGCAC 1 cut(s) 649
ApeKI GCWGC 6 cut(s) 286, 301, 422, 1090, 1346, 1349
ApoI RAATTY 4 cut(s) 295, 361, 464, 854
ArsI GACNNNNNNTTYG 4 cut(s) 468, 500, 989, 1021
AseI ATTAAT 1 cut(s) 1047
Asp700I GAANNNNTTC 4 cut(s) 257, 464, 934, 1196
AspA2I CCTAGG 1 cut(s) 986
AspLEI GCGC 1 cut(s) 889
AspS9I GGNCC 1 cut(s) 1108
AsuHPI GGTGA 4 cut(s) 320, 1007, 1252, 1253
AsuNHI GCTAGC 1 cut(s) 889
AvrII CCTAGG 1 cut(s) 986
BaeGI GKGCMC 1 cut(s) 653
BalI TGGCCA 1 cut(s) 870
BamHI GGATCC 2 cut(s) 46, 1391
BanI GGYRCC 1 cut(s) 1081
BanII GRGCYC 2 cut(s) 246, 518
BarI GAAGNNNNNNTAC 2 cut(s) 1124, 1156
Bbv12I GWGCWC 2 cut(s) 246, 653
BbvI GCAGC 6 cut(s) 273, 288, 434, 1077, 1358, 1361
BccI CCATC 4 cut(s) 401, 430, 496, 1219
BceAI ACGGC 1 cut(s) 791
BcgI CGANNNNNNTGC 4 cut(s) 220, 254, 623, 657
BciT130I CCWGG 3 cut(s) 670, 786, 1341
BciVI GTATCC 1 cut(s) 127
BfaI CTAG 4 cut(s) 488, 722, 890, 987
BfmI CTRYAG 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 1156
BfuI GTATCC 1 cut(s) 127
BisI GCNGC 7 cut(s) 287, 302, 423, 790, 1091, 1347, 1350
BlnI CCTAGG 1 cut(s) 986
BlpI GCTNAGC 1 cut(s) 793
BlsI GCNGC 7 cut(s) 288, 303, 424, 791, 1092, 1348, 1351
Bme1390I CCNGG 3 cut(s) 670, 786, 1341
BmgT120I GGNCC 1 cut(s) 1108
BmiI GGNNCC 4 cut(s) 48, 753, 1083, 1393
BmrFI CCNGG 3 cut(s) 670, 786, 1341
BmsI GCATC 3 cut(s) 402, 625, 631
BmtI GCTAGC 1 cut(s) 893
BpmI CTGGAG 1 cut(s) 275
Bpu1102I GCTNAGC 1 cut(s) 793
BpuEI CTTGAG 1 cut(s) 319
BsaAI YACGTR 1 cut(s) 575
BsaBI GATNNNNATC 2 cut(s) 80, 1390
BsaHI GRCGYC 1 cut(s) 440
BsaJI CCNNGG 6 cut(s) 138, 700, 784, 986, 1085, 1395
BsaWI WCCGGW 2 cut(s) 153, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 992
Bse1I ACTGG 4 cut(s) 93, 500, 871, 1287
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 2 cut(s) 80, 1390
BseAI TCCGGA 1 cut(s) 528
BseBI CCWGG 3 cut(s) 670, 786, 1341
BseDI CCNNGG 6 cut(s) 138, 700, 784, 986, 1085, 1395
BseGI GGATG 1 cut(s) 1405
BseJI GATNNNNATC 2 cut(s) 80, 1390
BseLI CCNNNNNNNGG 1 cut(s) 992
BseMI GCAATG 1 cut(s) 228
BseNI ACTGG 4 cut(s) 93, 500, 871, 1287
BseRI GAGGAG 2 cut(s) 121, 124
BseSI GKGCMC 1 cut(s) 653
BseXI GCAGC 6 cut(s) 273, 288, 434, 1077, 1358, 1361
BsgI GTGCAG 1 cut(s) 1184
Bsh1285I CGRYCG 1 cut(s) 592
BshFI GGCC 3 cut(s) 748, 870, 1109
BshNI GGYRCC 1 cut(s) 1081
BsiEI CGRYCG 1 cut(s) 592
BsiHKAI GWGCWC 2 cut(s) 246, 653
BsiSI CCGG 4 cut(s) 154, 529, 749, 755
BslFI GGGAC 3 cut(s) 827, 1293, 1344
BslI CCNNNNNNNGG 1 cut(s) 992
BsmFI GGGAC 3 cut(s) 827, 1293, 1344
BsmI GAATGC 1 cut(s) 1237
BsnI GGCC 3 cut(s) 748, 870, 1109
Bsp1286I GDGCHC 3 cut(s) 246, 518, 653
Bsp13I TCCGGA 1 cut(s) 528
Bsp143I GATC 6 cut(s) 46, 70, 237, 490, 696, 1391
Bsp1720I GCTNAGC 1 cut(s) 793
Bsp19I CCATGG 1 cut(s) 700
BspACI CCGC 1 cut(s) 789
BspANI GGCC 3 cut(s) 748, 870, 1109
BspEI TCCGGA 1 cut(s) 528
BspHI TCATGA 1 cut(s) 711
BspLI GGNNCC 4 cut(s) 48, 753, 1083, 1393
BspMI ACCTGC 1 cut(s) 1156
BspOI GCTAGC 1 cut(s) 893
BspPI GGATC 6 cut(s) 41, 54, 245, 691, 1386, 1399
BspQI GCTCTTC 1 cut(s) 1173
BspT107I GGYRCC 1 cut(s) 1081
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 4 cut(s) 93, 500, 871, 1287
BssECI CCNNGG 6 cut(s) 138, 700, 784, 986, 1085, 1395
BssMI GATC 6 cut(s) 46, 70, 237, 490, 696, 1391
BssNI GRCGYC 1 cut(s) 440
BssT1I CCWWGG 2 cut(s) 700, 986
Bst2UI CCWGG 3 cut(s) 670, 786, 1341
Bst4CI ACNGT 3 cut(s) 484, 593, 875
Bst6I CTCTTC 3 cut(s) 98, 130, 1173
BstACI GRCGYC 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 193
BstBAI YACGTR 1 cut(s) 575
BstC8I GCNNGC 1 cut(s) 891
BstDEI CTNAG 2 cut(s) 428, 793
BstDSI CCRYGG 1 cut(s) 700
BstF5I GGATG 1 cut(s) 1405
BstHHI GCGC 1 cut(s) 889
BstKTI GATC 6 cut(s) 49, 73, 240, 493, 699, 1394
BstMBI GATC 6 cut(s) 46, 70, 237, 490, 696, 1391
BstMCI CGRYCG 1 cut(s) 592
BstMWI GCNNNNNNNGC 4 cut(s) 193, 379, 803, 1090
BstNI CCWGG 3 cut(s) 670, 786, 1341
BstNSI RCATGY 3 cut(s) 40, 567, 820
BstSCI CCNGG 3 cut(s) 668, 784, 1339
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 653
BstV1I GCAGC 6 cut(s) 273, 288, 434, 1077, 1358, 1361
BstX2I RGATCY 3 cut(s) 46, 696, 1391
BstXI CCANNNNNNTGG 2 cut(s) 58, 1387
BstYI RGATCY 3 cut(s) 46, 696, 1391
BsuI GTATCC 1 cut(s) 127
BsuRI GGCC 3 cut(s) 748, 870, 1109
BtgI CCRYGG 1 cut(s) 700
BtsCI GGATG 1 cut(s) 1405
BtsIMutI CAGTG 3 cut(s) 493, 621, 651
BveI ACCTGC 1 cut(s) 1156
Cac8I GCNNGC 1 cut(s) 891
CciI TCATGA 1 cut(s) 711
CfoI GCGC 1 cut(s) 889
Cfr13I GGNCC 1 cut(s) 1108
CseI GACGC 1 cut(s) 429
Csp6I GTAC 1 cut(s) 1015
CviAII CATG 8 cut(s) 37, 401, 564, 645, 701, 712, 817, 1018
CviQI GTAC 1 cut(s) 1015
DdeI CTNAG 2 cut(s) 428, 793
DpnI GATC 6 cut(s) 48, 72, 239, 492, 698, 1393
DpnII GATC 6 cut(s) 46, 70, 237, 490, 696, 1391
EaeI YGGCCR 2 cut(s) 746, 868
Eam1104I CTCTTC 3 cut(s) 98, 130, 1173
EarI CTCTTC 3 cut(s) 98, 130, 1173
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 2 cut(s) 700, 986
Eco24I GRGCYC 2 cut(s) 246, 518
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 1108
EcoRII CCWGG 3 cut(s) 668, 784, 1339
EcoT14I CCWWGG 2 cut(s) 700, 986
EcoT22I ATGCAT 1 cut(s) 646
EcoT38I GRGCYC 2 cut(s) 246, 518
ErhI CCWWGG 2 cut(s) 700, 986
FaeI CATG 8 cut(s) 40, 404, 567, 648, 704, 715, 820, 1021
FalI AAGNNNNNCTT 2 cut(s) 1181, 1213
FaqI GGGAC 3 cut(s) 827, 1293, 1344
FatI CATG 8 cut(s) 36, 400, 563, 644, 700, 711, 816, 1017
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 7 cut(s) 287, 302, 423, 790, 1091, 1347, 1350
FriOI GRGCYC 2 cut(s) 246, 518
Fsp4HI GCNGC 7 cut(s) 287, 302, 423, 790, 1091, 1347, 1350
FspBI CTAG 4 cut(s) 488, 722, 890, 987
GlaI GCGC 1 cut(s) 888
GluI GCNGC 7 cut(s) 287, 302, 423, 790, 1091, 1347, 1350
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 3 cut(s) 748, 870, 1109
HapII CCGG 4 cut(s) 154, 529, 749, 755
HgaI GACGC 1 cut(s) 429
HhaI GCGC 1 cut(s) 889
Hin1I GRCGYC 1 cut(s) 440
Hin1II CATG 8 cut(s) 40, 404, 567, 648, 704, 715, 820, 1021
Hin6I GCGC 1 cut(s) 887
HinP1I GCGC 1 cut(s) 887
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 5 cut(s) 63, 76, 122, 332, 935
HpaII CCGG 4 cut(s) 154, 529, 749, 755
HphI GGTGA 4 cut(s) 320, 1007, 1252, 1253
Hpy166II GTNNAC 4 cut(s) 121, 651, 728, 1375
Hpy188I TCNGA 4 cut(s) 75, 692, 940, 1399
Hpy188III TCNNGA 6 cut(s) 292, 336, 529, 712, 1175, 1268
Hpy8I GTNNAC 4 cut(s) 121, 651, 728, 1375
HpyAV CCTTC 4 cut(s) 640, 911, 1270, 1289
HpyCH4III ACNGT 3 cut(s) 484, 593, 875
HpyCH4IV ACGT 2 cut(s) 341, 574
HpyCH4V TGCA 7 cut(s) 30, 187, 422, 638, 644, 651, 1165
HpyF10VI GCNNNNNNNGC 4 cut(s) 193, 379, 803, 1090
HpyF3I CTNAG 2 cut(s) 428, 793
HpySE526I ACGT 2 cut(s) 341, 574
Hsp92I GRCGYC 1 cut(s) 440
Hsp92II CATG 8 cut(s) 40, 404, 567, 648, 704, 715, 820, 1021
HspAI GCGC 1 cut(s) 887
Kpn2I TCCGGA 1 cut(s) 528
Kzo9I GATC 6 cut(s) 46, 70, 237, 490, 696, 1391
LguI GCTCTTC 1 cut(s) 1173
LmnI GCTCC 3 cut(s) 294, 751, 1343
Lsp1109I GCAGC 6 cut(s) 273, 288, 434, 1077, 1358, 1361
LweI GCATC 3 cut(s) 402, 625, 631
MaeI CTAG 4 cut(s) 488, 722, 890, 987
MaeII ACGT 2 cut(s) 341, 574
MaeIII GTNAC 5 cut(s) 11, 575, 593, 862, 995
MalI GATC 6 cut(s) 48, 72, 239, 492, 698, 1393
MboI GATC 6 cut(s) 46, 70, 237, 490, 696, 1391
MboII GAAGA 5 cut(s) 115, 117, 511, 1163, 1190
MflI RGATCY 3 cut(s) 46, 696, 1391
MhlI GDGCHC 3 cut(s) 246, 518, 653
MlsI TGGCCA 1 cut(s) 870
MluCI AATT 8 cut(s) 97, 295, 361, 464, 854, 894, 1048, 1192
MluNI TGGCCA 1 cut(s) 870
MlyI GAGTC 2 cut(s) 116, 326
MmeI TCCRAC 4 cut(s) 24, 670, 930, 939
Mox20I TGGCCA 1 cut(s) 870
Mph1103I ATGCAT 1 cut(s) 646
MroI TCCGGA 1 cut(s) 528
MroXI GAANNNNTTC 4 cut(s) 257, 464, 934, 1196
MscI TGGCCA 1 cut(s) 870
MseI TTAA 4 cut(s) 914, 1047, 1308, 1322
MslI CAYNNNNRTG 3 cut(s) 182, 405, 562
Msp20I TGGCCA 1 cut(s) 870
MspI CCGG 4 cut(s) 154, 529, 749, 755
MspR9I CCNGG 3 cut(s) 670, 786, 1341
Mva1269I GAATGC 1 cut(s) 1237
MvaI CCWGG 3 cut(s) 670, 786, 1341
MwoI GCNNNNNNNGC 4 cut(s) 193, 379, 803, 1090
NcoI CCATGG 1 cut(s) 700
NdeII GATC 6 cut(s) 46, 70, 237, 490, 696, 1391
NheI GCTAGC 1 cut(s) 889
NlaIII CATG 8 cut(s) 40, 404, 567, 648, 704, 715, 820, 1021
NlaIV GGNNCC 4 cut(s) 48, 753, 1083, 1393
NmeAIII GCCGAG 1 cut(s) 1110
NmuCI GTSAC 3 cut(s) 575, 593, 995
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 3 cut(s) 40, 567, 820
PagI TCATGA 1 cut(s) 711
PciI ACATGT 1 cut(s) 36
PciSI GCTCTTC 1 cut(s) 1173
PcsI WCGNNNNNNNCGW 1 cut(s) 347
PctI GAATGC 1 cut(s) 1237
PdmI GAANNNNTTC 4 cut(s) 257, 464, 934, 1196
PfeI GAWTC 3 cut(s) 63, 76, 935
PkrI GCNGC 7 cut(s) 288, 303, 424, 791, 1092, 1348, 1351
PleI GAGTC 2 cut(s) 116, 326
PpsI GAGTC 2 cut(s) 116, 326
Ppu21I YACGTR 1 cut(s) 575
PscI ACATGT 1 cut(s) 36
PshBI ATTAAT 1 cut(s) 1047
Psp124BI GAGCTC 1 cut(s) 246
Psp1406I AACGTT 1 cut(s) 341
Psp6I CCWGG 3 cut(s) 668, 784, 1339
PspGI CCWGG 3 cut(s) 668, 784, 1339
PspN4I GGNNCC 4 cut(s) 48, 753, 1083, 1393
PspPI GGNCC 1 cut(s) 1108
PsrI GAACNNNNNNTAC 2 cut(s) 452, 484
PsuI RGATCY 3 cut(s) 46, 696, 1391
RsaI GTAC 1 cut(s) 1016
RsaNI GTAC 1 cut(s) 1015
RseI CAYNNNNRTG 3 cut(s) 182, 405, 562
SacI GAGCTC 1 cut(s) 246
SalI GTCGAC 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 1173
SaqAI TTAA 4 cut(s) 914, 1047, 1308, 1322
SatI GCNGC 7 cut(s) 287, 302, 423, 790, 1091, 1347, 1350
Sau3AI GATC 6 cut(s) 46, 70, 237, 490, 696, 1391
Sau96I GGNCC 1 cut(s) 1108
SchI GAGTC 2 cut(s) 116, 326
ScrFI CCNGG 3 cut(s) 670, 786, 1341
SduI GDGCHC 3 cut(s) 246, 518, 653
SfaNI GCATC 3 cut(s) 402, 625, 631
SfcI CTRYAG 1 cut(s) 480
SmiMI CAYNNNNRTG 3 cut(s) 182, 405, 562
SmlI CTYRAG 1 cut(s) 334
SmoI CTYRAG 1 cut(s) 334
Sse9I AATT 8 cut(s) 97, 295, 361, 464, 854, 894, 1048, 1192
SsiI CCGC 1 cut(s) 789
SspMI CTAG 4 cut(s) 488, 722, 890, 987
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 3 cut(s) 668, 784, 1339
StyI CCWWGG 2 cut(s) 700, 986
TaaI ACNGT 3 cut(s) 484, 593, 875
TaiI ACGT 2 cut(s) 344, 577
TaqI TCGA 5 cut(s) 120, 240, 474, 551, 633
TasI AATT 8 cut(s) 97, 295, 361, 464, 854, 894, 1048, 1192
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 1 cut(s) 792
TfiI GAWTC 3 cut(s) 63, 76, 935
Tru1I TTAA 4 cut(s) 914, 1047, 1308, 1322
Tru9I TTAA 4 cut(s) 914, 1047, 1308, 1322
TscAI CASTG 3 cut(s) 500, 628, 658
TseFI GTSAC 3 cut(s) 575, 593, 995
TseI GCWGC 6 cut(s) 286, 301, 422, 1090, 1346, 1349
Tsp45I GTSAC 3 cut(s) 575, 593, 995
TspDTI ATGAA 5 cut(s) 343, 700, 728, 1046, 1173
TspRI CASTG 3 cut(s) 500, 628, 658
VneI GTGCAC 1 cut(s) 649
VspI ATTAAT 1 cut(s) 1047
XapI RAATTY 4 cut(s) 295, 361, 464, 854
XceI RCATGY 3 cut(s) 40, 567, 820
XcmI CCANNNNNNNNNTGG 1 cut(s) 878
XmaJI CCTAGG 1 cut(s) 986
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 4 cut(s) 257, 464, 934, 1196
XspI CTAG 4 cut(s) 488, 722, 890, 987
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.