MD02G1172900.v1.1

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003099
Physical Location & Seq
Reverse (-)
15139775 .. 15144103
4329 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1172900.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGGCCTGCGACAAGGGATTTTCGAGTAGCTATTTTCTACTGAAGCCAGAAGAAGTTGGCTTCTTTGATCTGGTTCACATTCTGTTCTCCTCCAACGTTGAGGAAGGAAGGTATGCAGATTACTCTAAGAGTGAGGGCACAGTCACAGATGATACGATCAGCTTCAGACGCAGATGGCTTATGTTTATCTCCATCTTGGCTCAAAAGTTTCTGCAACTTGTGTCAAAGCCACTCTCAGTTTTAGGGTCGGGGATTGAAATGTGTTTGAACCTTTTGTCAAGCAACCGCAATATTTTTGTGCTCGTACTCAATTCCTTCCGAGGGAAGTTGGTAAAGCCAGATAAATCATCAGCAACATTTCTGTCATTTATAGGGAATATGGACAAGCGGGTGAAGTTAGACAGTAGCATCAAACATGATGATGCCAATTACCATGCAGCACTCTCTATGATGGCTTCTAAAGCTTCTTATGAAAACAAGACCTACATTGAAACTACTGTGAATGATCACTGGAAGATGGAGTTCTTGGGATTTTATGACTTCTGGAATGATTATCAACAAAAAGCCACAACACAAGCCTTCTTGCTTCGTGATAAAACTGGTGACCGTGACACCATTGTTGTGGCCTTTAGGGGAACCGAACCTTTCGACGCCGATGCATGGTGCTCCGACTTTGACATCTCATGGTACGAGCTTTCAGCCGGGGTTGGAAAAATTCATGGTGGCTTTATGAAAGCTTTAGGCTTACAAAAGAATGTTGGTTGGCCTAAAGAAATCAAACAAGAAGATGGGCAGCCAGCCGTAGCTTACTATGCCATTAGAGAGATGCTAAAAGAACTTTTGAGCCAAAACGATCAAGCGAGATACATTGTGACAGGGCATAGTTTGGGTGGGGCTCTAGCAATTCTGTTTCCTACAGTTCTGGCTCTGCACAATGAGGCATGGTTATTGGAGGGGTTGCAAGGGGTGTATACATTTGGGCAGCCAAGGGTAGGAGATGAGAAATTTGGAGAGTTCATGACAGAAAAATTGAAAGAGCATGGTATTCGATATTTTAGATTCGTTTATGGTAATGATATGGTGCCTAGGTTGCCTTATGATGACAAGGACTTAATGTTCAAGCATTTTGGGACATGCCTCTACTATAATCGGCAGTATGAAGTGAAGGTTGTTGCAGAAGAACCGAACAAGAATTACTTTTCACCATTGGGGGCAATACCAATGATGATAAATGCCTTCTGCGAGCTGATTAGAAGCTTCACTATTTCACGCACAAAGGGACCAGACTATAAAGAAGGATCTTTTCTAAGAATTTTTAGGGTCATTGGCTTGGTACTTCCAGGCGTTCCATCTCATAGCACCCAAGATTATGTTAATGCTACCCGGTTGGGATCATCAAACGTATTCCTCCCACCCAGAAACATACCGCAAGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

480

Amino Acids

54.54

Weight (kDa)

6.58

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 207 - 367 4.5e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1081
AccI GTMKAC 1 cut(s) 971
AciI CCGC 3 cut(s) 286, 388, 1427
AclI AACGTT 1 cut(s) 96
AclWI GGATC 2 cut(s) 1306, 1399
AcsI RAATTY 3 cut(s) 714, 1004, 1311
AcuI CTGAAG 2 cut(s) 62, 148
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 3 cut(s) 306, 689, 1335
AfiI CCNNNNNNNGG 3 cut(s) 321, 660, 992
AgsI TTSAA 5 cut(s) 257, 268, 491, 1033, 1120
AjnI CCWGG 1 cut(s) 1339
AloI GAACNNNNNNTCC 2 cut(s) 1100, 1132
AluBI AGCT 8 cut(s) 30, 162, 464, 694, 737, 806, 1246, 1257
AluI AGCT 8 cut(s) 30, 162, 464, 694, 737, 806, 1246, 1257
Alw21I GWGCWC 2 cut(s) 303, 668
AlwI GGATC 2 cut(s) 1306, 1399
AoxI GGCC 3 cut(s) 3, 624, 764
ApeKI GCWGC 3 cut(s) 437, 793, 982
ApoI RAATTY 3 cut(s) 714, 1004, 1311
AspA2I CCTAGG 1 cut(s) 1085
AspS9I GGNCC 1 cut(s) 1280
AsuC2I CCSGG 2 cut(s) 703, 1384
AsuHPI GGTGA 3 cut(s) 403, 614, 1194
AvaII GGWCC 1 cut(s) 1280
AvrII CCTAGG 1 cut(s) 1085
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 1081
BanII GRGCYC 1 cut(s) 898
Bbv12I GWGCWC 2 cut(s) 303, 668
BbvI GCAGC 3 cut(s) 449, 805, 994
BccI CCATC 6 cut(s) 168, 200, 445, 511, 782, 1357
BceAI ACGGC 1 cut(s) 785
BcgI CGANNNNNNTGC 4 cut(s) 638, 672, 1028, 1062
BciT130I CCWGG 1 cut(s) 1341
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 2 cut(s) 703, 1384
BfaI CTAG 2 cut(s) 899, 1086
BfmI CTRYAG 1 cut(s) 915
BisI GCNGC 3 cut(s) 438, 794, 983
BlnI CCTAGG 1 cut(s) 1085
BlsI GCNGC 3 cut(s) 439, 795, 984
Bme1390I CCNGG 3 cut(s) 703, 1341, 1384
Bme18I GGWCC 1 cut(s) 1280
BmgT120I GGNCC 1 cut(s) 1280
BmiI GGNNCC 3 cut(s) 637, 1083, 1281
BmrFI CCNGG 3 cut(s) 703, 1341, 1384
BmsI GCATC 4 cut(s) 412, 417, 646, 816
BpuMI CCSGG 2 cut(s) 703, 1384
BsaHI GRCGYC 1 cut(s) 651
BsaJI CCNNGG 4 cut(s) 319, 702, 986, 1085
Bsc4I CCNNNNNNNGG 3 cut(s) 321, 660, 992
Bse1I ACTGG 2 cut(s) 515, 604
BseBI CCWGG 1 cut(s) 1341
BseDI CCNNGG 4 cut(s) 319, 702, 986, 1085
BseLI CCNNNNNNNGG 3 cut(s) 321, 660, 992
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 2 cut(s) 515, 604
BseRI GAGGAG 1 cut(s) 79
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 3 cut(s) 449, 805, 994
BsgI GTGCAG 1 cut(s) 914
BshFI GGCC 3 cut(s) 5, 626, 766
BshNI GGYRCC 1 cut(s) 1081
BsiHKAI GWGCWC 2 cut(s) 303, 668
BsiSI CCGG 2 cut(s) 702, 1384
BslFI GGGAC 2 cut(s) 1144, 1293
BslI CCNNNNNNNGG 3 cut(s) 321, 660, 992
BsmFI GGGAC 2 cut(s) 1144, 1293
BsnI GGCC 3 cut(s) 5, 626, 766
Bsp1286I GDGCHC 4 cut(s) 140, 303, 668, 898
Bsp143I GATC 6 cut(s) 67, 156, 505, 853, 1298, 1391
BspACI CCGC 3 cut(s) 286, 388, 1427
BspANI GGCC 3 cut(s) 5, 626, 766
BspCNI CTCAG 1 cut(s) 248
BspHI TCATGA 1 cut(s) 1017
BspLI GGNNCC 3 cut(s) 637, 1083, 1281
BspPI GGATC 2 cut(s) 1306, 1399
BspT107I GGYRCC 1 cut(s) 1081
BsrI ACTGG 2 cut(s) 515, 604
BssECI CCNNGG 4 cut(s) 319, 702, 986, 1085
BssMI GATC 6 cut(s) 67, 156, 505, 853, 1298, 1391
BssNAI GTATAC 1 cut(s) 972
BssNI GRCGYC 1 cut(s) 651
BssT1I CCWWGG 2 cut(s) 986, 1085
Bst1107I GTATAC 1 cut(s) 972
Bst2UI CCWGG 1 cut(s) 1341
Bst4CI ACNGT 5 cut(s) 142, 404, 499, 608, 919
BstACI GRCGYC 1 cut(s) 651
BstC8I GCNNGC 3 cut(s) 7, 798, 1244
BstDEI CTNAG 3 cut(s) 126, 235, 1307
BstEII GGTNACC 1 cut(s) 602
BstKTI GATC 6 cut(s) 70, 159, 508, 856, 1301, 1394
BstMBI GATC 6 cut(s) 67, 156, 505, 853, 1298, 1391
BstMWI GCNNNNNNNGC 4 cut(s) 168, 461, 812, 1090
BstNI CCWGG 1 cut(s) 1341
BstNSI RCATGY 1 cut(s) 1137
BstPI GGTNACC 1 cut(s) 602
BstSCI CCNGG 3 cut(s) 701, 1339, 1382
BstSFI CTRYAG 1 cut(s) 915
BstSLI GKGCMC 1 cut(s) 140
BstV1I GCAGC 3 cut(s) 449, 805, 994
BstX2I RGATCY 1 cut(s) 1298
BstXI CCANNNNNNTGG 1 cut(s) 622
BstYI RGATCY 1 cut(s) 1298
BstZ17I GTATAC 1 cut(s) 972
BsuRI GGCC 3 cut(s) 5, 626, 766
BtsIMutI CAGTG 1 cut(s) 508
Cac8I GCNNGC 3 cut(s) 7, 798, 1244
CciI TCATGA 1 cut(s) 1017
Cfr13I GGNCC 1 cut(s) 1280
CseI GACGC 2 cut(s) 177, 659
Csp6I GTAC 3 cut(s) 305, 688, 1334
CviAII CATG 9 cut(s) 416, 434, 660, 684, 719, 942, 1018, 1040, 1134
CviQI GTAC 3 cut(s) 305, 688, 1334
DdeI CTNAG 3 cut(s) 126, 235, 1307
DpnI GATC 6 cut(s) 69, 158, 507, 855, 1300, 1393
DpnII GATC 6 cut(s) 67, 156, 505, 853, 1298, 1391
Eco130I CCWWGG 2 cut(s) 986, 1085
Eco24I GRGCYC 1 cut(s) 898
Eco47I GGWCC 1 cut(s) 1280
Eco57I CTGAAG 2 cut(s) 62, 148
Eco91I GGTNACC 1 cut(s) 602
EcoO65I GGTNACC 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 1339
EcoT14I CCWWGG 2 cut(s) 986, 1085
EcoT22I ATGCAT 1 cut(s) 661
EcoT38I GRGCYC 1 cut(s) 898
ErhI CCWWGG 2 cut(s) 986, 1085
FaeI CATG 9 cut(s) 419, 437, 663, 687, 722, 945, 1021, 1043, 1137
FalI AAGNNNNNCTT 2 cut(s) 1181, 1213
FaqI GGGAC 2 cut(s) 1144, 1293
FatI CATG 9 cut(s) 415, 433, 659, 683, 718, 941, 1017, 1039, 1133
FauI CCCGC 1 cut(s) 381
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 1 cut(s) 971
Fnu4HI GCNGC 3 cut(s) 438, 794, 983
FriOI GRGCYC 1 cut(s) 898
Fsp4HI GCNGC 3 cut(s) 438, 794, 983
FspBI CTAG 2 cut(s) 899, 1086
GluI GCNGC 3 cut(s) 438, 794, 983
HaeIII GGCC 3 cut(s) 5, 626, 766
HapII CCGG 2 cut(s) 702, 1384
HgaI GACGC 2 cut(s) 177, 659
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 9 cut(s) 419, 437, 663, 687, 722, 945, 1021, 1043, 1137
HindIII AAGCTT 3 cut(s) 462, 735, 1255
HinfI GANTC 1 cut(s) 1059
HpaII CCGG 2 cut(s) 702, 1384
HphI GGTGA 3 cut(s) 403, 614, 1194
Hpy166II GTNNAC 2 cut(s) 76, 972
Hpy188I TCNGA 3 cut(s) 167, 320, 670
Hpy188III TCNNGA 3 cut(s) 544, 590, 1018
Hpy8I GTNNAC 2 cut(s) 76, 972
Hpy99I CGWCG 1 cut(s) 653
HpyAV CCTTC 7 cut(s) 98, 102, 325, 589, 1159, 1246, 1289
HpyCH4III ACNGT 5 cut(s) 142, 404, 499, 608, 919
HpyCH4IV ACGT 2 cut(s) 96, 1401
HpyCH4V TGCA 7 cut(s) 116, 214, 437, 659, 931, 961, 1175
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 461, 812, 1090
HpyF3I CTNAG 3 cut(s) 126, 235, 1307
HpySE526I ACGT 2 cut(s) 96, 1401
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 9 cut(s) 419, 437, 663, 687, 722, 945, 1021, 1043, 1137
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 6 cut(s) 67, 156, 505, 853, 1298, 1391
LmnI GCTCC 1 cut(s) 671
Lsp1109I GCAGC 3 cut(s) 449, 805, 994
LweI GCATC 4 cut(s) 412, 417, 646, 816
MaeI CTAG 2 cut(s) 899, 1086
MaeII ACGT 2 cut(s) 96, 1401
MaeIII GTNAC 4 cut(s) 142, 602, 608, 871
MalI GATC 6 cut(s) 69, 158, 507, 855, 1300, 1393
MboI GATC 6 cut(s) 67, 156, 505, 853, 1298, 1391
MboII GAAGA 4 cut(s) 62, 526, 797, 1190
MflI RGATCY 1 cut(s) 1298
MhlI GDGCHC 4 cut(s) 140, 303, 668, 898
MluCI AATT 8 cut(s) 310, 427, 714, 903, 1004, 1028, 1192, 1311
MmeI TCCRAC 3 cut(s) 117, 688, 693
MnlI CCTC 8 cut(s) 94, 100, 127, 314, 931, 946, 1148, 1418
Mph1103I ATGCAT 1 cut(s) 661
MseI TTAA 2 cut(s) 1112, 1374
MslI CAYNNNNRTG 2 cut(s) 420, 620
MspI CCGG 2 cut(s) 702, 1384
MspR9I CCNGG 3 cut(s) 703, 1341, 1384
MvaI CCWGG 1 cut(s) 1341
MwoI GCNNNNNNNGC 4 cut(s) 168, 461, 812, 1090
NciI CCSGG 2 cut(s) 703, 1384
NdeII GATC 6 cut(s) 67, 156, 505, 853, 1298, 1391
NlaIII CATG 9 cut(s) 419, 437, 663, 687, 722, 945, 1021, 1043, 1137
NlaIV GGNNCC 3 cut(s) 637, 1083, 1281
NmuCI GTSAC 4 cut(s) 142, 602, 608, 871
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 1 cut(s) 1137
PagI TCATGA 1 cut(s) 1017
PfeI GAWTC 1 cut(s) 1059
PkrI GCNGC 3 cut(s) 439, 795, 984
Psp1406I AACGTT 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 1339
PspEI GGTNACC 1 cut(s) 602
PspGI CCWGG 1 cut(s) 1339
PspN4I GGNNCC 3 cut(s) 637, 1083, 1281
PspPI GGNCC 1 cut(s) 1280
PsrI GAACNNNNNNTAC 2 cut(s) 1178, 1210
PsuI RGATCY 1 cut(s) 1298
RsaI GTAC 3 cut(s) 306, 689, 1335
RsaNI GTAC 3 cut(s) 305, 688, 1334
RseI CAYNNNNRTG 2 cut(s) 420, 620
SaqAI TTAA 2 cut(s) 1112, 1374
SatI GCNGC 3 cut(s) 438, 794, 983
Sau3AI GATC 6 cut(s) 67, 156, 505, 853, 1298, 1391
Sau96I GGNCC 1 cut(s) 1280
ScrFI CCNGG 3 cut(s) 703, 1341, 1384
SduI GDGCHC 4 cut(s) 140, 303, 668, 898
SfaNI GCATC 4 cut(s) 412, 417, 646, 816
SfcI CTRYAG 1 cut(s) 915
SinI GGWCC 1 cut(s) 1280
SmiMI CAYNNNNRTG 2 cut(s) 420, 620
Sse9I AATT 8 cut(s) 310, 427, 714, 903, 1004, 1028, 1192, 1311
SsiI CCGC 3 cut(s) 286, 388, 1427
SspI AATATT 1 cut(s) 292
SspMI CTAG 2 cut(s) 899, 1086
StyD4I CCNGG 3 cut(s) 701, 1339, 1382
StyI CCWWGG 2 cut(s) 986, 1085
TaaI ACNGT 5 cut(s) 142, 404, 499, 608, 919
TaiI ACGT 2 cut(s) 99, 1404
TaqI TCGA 3 cut(s) 23, 648, 1048
TasI AATT 8 cut(s) 310, 427, 714, 903, 1004, 1028, 1192, 1311
TfiI GAWTC 1 cut(s) 1059
Tru1I TTAA 2 cut(s) 1112, 1374
Tru9I TTAA 2 cut(s) 1112, 1374
TscAI CASTG 1 cut(s) 515
TseFI GTSAC 4 cut(s) 142, 602, 608, 871
TseI GCWGC 3 cut(s) 437, 793, 982
Tsp45I GTSAC 4 cut(s) 142, 602, 608, 871
TspDTI ATGAA 5 cut(s) 486, 707, 746, 1006, 1173
TspRI CASTG 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 1280
XapI RAATTY 3 cut(s) 714, 1004, 1311
XceI RCATGY 1 cut(s) 1137
XmaJI CCTAGG 1 cut(s) 1085
XmiI GTMKAC 1 cut(s) 971
XspI CTAG 2 cut(s) 899, 1086
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.