Rw2G014200

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003099
Physical Location & Seq
Reverse (-)
15753966 .. 15757039
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014200.1

Sequence Viewer

Length: 1422 bp
ATGGCTTGTAACAAGAGTTTTTCCAGCAATTACATGCTACTGGACCCCAACGATGTGGGTTTCTTTGATCTGATTCACATCTTGATCTCCAATAACTTGGAGAAGAGAAAGTTCGTTGACTCCTCCGATGACAAAGAAGATGACTTTCAGCGCAGATGGCTCATATTTCTGTCTATTGTGGTGCAGAAGTTGCTGCAGTCTGTGTCGAAGCCAATGGCAGTTATTGGCTGGGCAATCGAAATGTTCTTGAACTTTTTGTCAATTAATTGCTGCAGCTTGCATGGACTGCTTCTAAATCTTTTGCGAGGCAAGATCATAGTACCAGATAGCAACTCAGAAACATTCTTGTCGTTTATTGGAAATTTGGATAAGCGAGTAAAGCTGGACAGTAGAATCAAACCTGGAGATGGCAAATATAATGCAGCACTCAGTGTGATGGCCTCTAAACTATCATATGAGAACCATGCCTGCATCAAAACTACAGTCCAAGATCACTGGAAGATGGAGTTCGTGGGGTTTTACGACTTCTGGAATGAGTATCAGGGAAAAGCTACAACACAAGCCTTTATGCTTCGGGACAGATCACGTGCTGACCATGATACCATTGTTGTTGCCTTTAGAGGAACCGAACCCTTTGATGCAGATGCATGGTGCTCTGACTTTGATATTTCGTGGTATGAACTTGAAGGTGGAGTTAACGGAAAGATCCATGGTGGCTTCATGAAAGCTCTAGGTTTACAGAAAAATATCGGTTGGCCAAAGAAGATCGAGCAAGATGACAAGCACCCCTTAGCTTACTATGCTGTTAGGGACATGCTGAAAAAGCTACTAAGTGAAAATGATAAGGCGAGATATATCATAACAGGGCACAGTTTAGGCGGAGCGTTAGCAATTCTCTTCCCTGCAATTTTGGCATTGCACGAGGAAAACGAGCTGTTGGAGAAATTGGAAGGAGTTTATACATTTGGGCAGCCTAGGGTTGGAGACAAGGAATTTGGGCAATACATGGAAAATGAAGTATTGAAAGAGCACAATGTTAGGTATTTTAGGTTTGTTTATGGTAATGATTTGGTGCCCAGGTTACCCTATGATGACAAGGCCCTCATGTTCAAGCACTTTGGGACCTGCCTCTACTATAACAGAAACTATGAAGCGCAGATTCTTCCAGAAGAGCCAAACAAGAATTACTTCTCCCCATCGGTGCAAATAACTATGATGGTGAATGCATTTTATGAGCTGACGAGAAGCTTCACACTTCCAAATAAGTACGGACCTGACTACGTCGAAGGGTACTTCTTCAGATTGTTTAGGCTAAGTGGTTTGCTAATGCCAGGGGCATCAGCACATTGTCCTCAAGATTATGTGAATTTAACCAGATTGGGACCCTCAGATGTGTTCGTCTCACCTACAAGCAACTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

473

Amino Acids

54.3

Weight (kDa)

6.09

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 203 - 364 2.7e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1133
AccB1I GGYRCC 1 cut(s) 1072
AciI CCGC 1 cut(s) 879
AclWI GGATC 1 cut(s) 700
AcoI YGGCCR 1 cut(s) 755
AcsI RAATTY 3 cut(s) 361, 992, 1366
AcuI CTGAAG 1 cut(s) 1282
AcvI CACGTG 1 cut(s) 587
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 3 cut(s) 321, 1268, 1292
AfiI CCNNNNNNNGG 2 cut(s) 407, 980
AgsI TTSAA 4 cut(s) 250, 686, 1024, 1111
AjnI CCWGG 3 cut(s) 400, 1076, 1330
AluBI AGCT 9 cut(s) 276, 382, 551, 728, 794, 826, 934, 1237, 1248
AluI AGCT 9 cut(s) 276, 382, 551, 728, 794, 826, 934, 1237, 1248
Alw21I GWGCWC 2 cut(s) 656, 1032
Alw26I GTCTC 2 cut(s) 978, 1405
AlwI GGATC 1 cut(s) 700
AoxI GGCC 3 cut(s) 438, 755, 1098
ApeKI GCWGC 5 cut(s) 193, 270, 273, 422, 970
ApoI RAATTY 3 cut(s) 361, 992, 1366
ArsI GACNNNNNNTTYG 4 cut(s) 468, 500, 977, 1009
AseI ATTAAT 1 cut(s) 264
Asp700I GAANNNNTTC 1 cut(s) 1187
AspA2I CCTAGG 1 cut(s) 974
AspLEI GCGC 2 cut(s) 153, 1156
AspS9I GGNCC 5 cut(s) 43, 1099, 1122, 1271, 1382
AsuHPI GGTGA 2 cut(s) 1231, 1395
AvaII GGWCC 4 cut(s) 43, 1122, 1271, 1382
AvrII CCTAGG 1 cut(s) 974
BaeGI GKGCMC 2 cut(s) 870, 1077
BaeI ACNNNNGTAYC 2 cut(s) 591, 624
BalI TGGCCA 1 cut(s) 757
BanI GGYRCC 1 cut(s) 1072
BauI CACGAG 1 cut(s) 920
BbrPI CACGTG 1 cut(s) 587
Bbv12I GWGCWC 2 cut(s) 656, 1032
BbvI GCAGC 5 cut(s) 180, 257, 285, 434, 982
BccI CCATC 6 cut(s) 150, 401, 430, 496, 1204, 1210
BciT130I CCWGG 3 cut(s) 402, 1078, 1332
BcoDI GTCTC 2 cut(s) 978, 1405
BfaI CTAG 2 cut(s) 731, 975
BfmI CTRYAG 3 cut(s) 194, 271, 480
BfuAI ACCTGC 1 cut(s) 1133
BisI GCNGC 5 cut(s) 194, 271, 274, 423, 971
BlnI CCTAGG 1 cut(s) 974
BlsI GCNGC 5 cut(s) 195, 272, 275, 424, 972
Bme1390I CCNGG 3 cut(s) 402, 1078, 1332
Bme18I GGWCC 4 cut(s) 43, 1122, 1271, 1382
BmgT120I GGNCC 5 cut(s) 43, 1099, 1122, 1271, 1382
BmiI GGNNCC 6 cut(s) 45, 625, 1074, 1123, 1383, 1384
BmrFI CCNGG 3 cut(s) 402, 1078, 1332
BmsI GCATC 4 cut(s) 480, 628, 634, 1346
BpmI CTGGAG 1 cut(s) 423
Bpu10I CCTNAGC 1 cut(s) 790
BpuEI CTTGAG 1 cut(s) 1338
BsaAI YACGTR 1 cut(s) 587
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 709, 974, 1076, 1331
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 980
Bse1I ACTGG 2 cut(s) 45, 500
Bse3DI GCAATG 1 cut(s) 914
Bse8I GATNNNNATC 1 cut(s) 77
BseBI CCWGG 3 cut(s) 402, 1078, 1332
BseDI CCNNGG 4 cut(s) 709, 974, 1076, 1331
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 407, 980
BseMI GCAATG 1 cut(s) 914
BseMII CTCAG 3 cut(s) 348, 442, 1401
BseNI ACTGG 2 cut(s) 45, 500
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 2 cut(s) 870, 1077
BseXI GCAGC 5 cut(s) 180, 257, 285, 434, 982
BseYI CCCAGC 1 cut(s) 228
BsgI GTGCAG 1 cut(s) 203
BshFI GGCC 3 cut(s) 440, 757, 1100
BshNI GGYRCC 1 cut(s) 1072
BsiHKAI GWGCWC 2 cut(s) 656, 1032
BslFI GGGAC 4 cut(s) 590, 824, 1135, 1395
BslI CCNNNNNNNGG 2 cut(s) 407, 980
BsmAI GTCTC 2 cut(s) 978, 1405
BsmBI CGTCTC 1 cut(s) 1405
BsmFI GGGAC 4 cut(s) 590, 824, 1135, 1395
BsmI GAATGC 1 cut(s) 1228
BsnI GGCC 3 cut(s) 440, 757, 1100
Bsp1286I GDGCHC 4 cut(s) 656, 870, 1032, 1077
Bsp143I GATC 7 cut(s) 67, 84, 312, 490, 581, 705, 765
Bsp19I CCATGG 1 cut(s) 709
BspACI CCGC 1 cut(s) 879
BspANI GGCC 3 cut(s) 440, 757, 1100
BspCNI CTCAG 3 cut(s) 347, 441, 1400
BspHI TCATGA 1 cut(s) 720
BspLI GGNNCC 6 cut(s) 45, 625, 1074, 1123, 1383, 1384
BspMAI CTGCAG 2 cut(s) 198, 275
BspMI ACCTGC 1 cut(s) 1133
BspPI GGATC 1 cut(s) 700
BspQI GCTCTTC 1 cut(s) 1164
BspT107I GGYRCC 1 cut(s) 1072
BsrDI GCAATG 1 cut(s) 914
BsrI ACTGG 2 cut(s) 45, 500
BssECI CCNNGG 4 cut(s) 709, 974, 1076, 1331
BssMI GATC 7 cut(s) 67, 84, 312, 490, 581, 705, 765
BssSI CACGAG 1 cut(s) 920
BssT1I CCWWGG 2 cut(s) 709, 974
Bst2BI CACGAG 1 cut(s) 920
Bst2UI CCWGG 3 cut(s) 402, 1078, 1332
Bst4CI ACNGT 4 cut(s) 389, 484, 872, 1418
Bst6I CTCTTC 3 cut(s) 98, 902, 1164
BstAPI GCANNNNNTGC 2 cut(s) 190, 286
BstBAI YACGTR 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 278, 469
BstDEI CTNAG 6 cut(s) 334, 428, 790, 830, 1313, 1387
BstDSI CCRYGG 1 cut(s) 709
BstEII GGTNACC 1 cut(s) 1080
BstHHI GCGC 2 cut(s) 153, 1156
BstKTI GATC 7 cut(s) 70, 87, 315, 493, 584, 708, 768
BstMAI GTCTC 2 cut(s) 978, 1405
BstMBI GATC 7 cut(s) 67, 84, 312, 490, 581, 705, 765
BstMWI GCNNNNNNNGC 7 cut(s) 157, 190, 286, 379, 800, 823, 911
BstNI CCWGG 3 cut(s) 402, 1078, 1332
BstNSI RCATGY 2 cut(s) 37, 817
BstPI GGTNACC 1 cut(s) 1080
BstSCI CCNGG 3 cut(s) 400, 1076, 1330
BstSFI CTRYAG 3 cut(s) 194, 271, 480
BstSLI GKGCMC 2 cut(s) 870, 1077
BstV1I GCAGC 5 cut(s) 180, 257, 285, 434, 982
BstX2I RGATCY 1 cut(s) 705
BstXI CCANNNNNNTGG 2 cut(s) 55, 97
BstYI RGATCY 1 cut(s) 705
BsuRI GGCC 3 cut(s) 440, 757, 1100
BtgI CCRYGG 1 cut(s) 709
BtsIMutI CAGTG 2 cut(s) 436, 493
BveI ACCTGC 1 cut(s) 1133
Cac8I GCNNGC 2 cut(s) 278, 469
CciI TCATGA 1 cut(s) 720
CfoI GCGC 2 cut(s) 153, 1156
Cfr13I GGNCC 5 cut(s) 43, 1099, 1122, 1271, 1382
Csp6I GTAC 3 cut(s) 320, 1267, 1291
CviQI GTAC 3 cut(s) 320, 1267, 1291
DdeI CTNAG 6 cut(s) 334, 428, 790, 830, 1313, 1387
DpnI GATC 7 cut(s) 69, 86, 314, 492, 583, 707, 767
DpnII GATC 7 cut(s) 67, 84, 312, 490, 581, 705, 765
DraIII CACNNNGTG 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 755
Eam1104I CTCTTC 3 cut(s) 98, 902, 1164
EarI CTCTTC 3 cut(s) 98, 902, 1164
EciI GGCGGA 1 cut(s) 894
Eco130I CCWWGG 2 cut(s) 709, 974
Eco47I GGWCC 4 cut(s) 43, 1122, 1271, 1382
Eco57I CTGAAG 1 cut(s) 1282
Eco72I CACGTG 1 cut(s) 587
Eco91I GGTNACC 1 cut(s) 1080
EcoO109I RGGNCCY 3 cut(s) 1099, 1122, 1382
EcoO65I GGTNACC 1 cut(s) 1080
EcoRII CCWGG 3 cut(s) 400, 1076, 1330
EcoT14I CCWWGG 2 cut(s) 709, 974
EcoT22I ATGCAT 2 cut(s) 649, 1228
ErhI CCWWGG 2 cut(s) 709, 974
Esp3I CGTCTC 1 cut(s) 1405
FalI AAGNNNNNCTT 4 cut(s) 773, 805, 1172, 1204
FaqI GGGAC 4 cut(s) 590, 824, 1135, 1395
FauNDI CATATG 1 cut(s) 454
Fnu4HI GCNGC 5 cut(s) 194, 271, 274, 423, 971
Fsp4HI GCNGC 5 cut(s) 194, 271, 274, 423, 971
FspBI CTAG 2 cut(s) 731, 975
GlaI GCGC 2 cut(s) 152, 1155
GluI GCNGC 5 cut(s) 194, 271, 274, 423, 971
GsaI CCCAGC 1 cut(s) 232
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 3 cut(s) 440, 757, 1100
HhaI GCGC 2 cut(s) 153, 1156
Hin6I GCGC 2 cut(s) 151, 1154
HinP1I GCGC 2 cut(s) 151, 1154
HincII GTYRAC 2 cut(s) 118, 697
HindII GTYRAC 2 cut(s) 118, 697
HindIII AAGCTT 1 cut(s) 1246
HinfI GANTC 4 cut(s) 73, 119, 393, 1159
HpaI GTTAAC 1 cut(s) 697
HphI GGTGA 2 cut(s) 1231, 1395
Hpy166II GTNNAC 3 cut(s) 118, 697, 737
Hpy188I TCNGA 6 cut(s) 72, 127, 337, 658, 1301, 1390
Hpy188III TCNNGA 7 cut(s) 82, 247, 529, 575, 721, 1166, 1355
Hpy8I GTNNAC 3 cut(s) 118, 697, 737
Hpy99I CGWCG 1 cut(s) 1286
HpyAV CCTTC 3 cut(s) 680, 944, 1280
HpyCH4III ACNGT 4 cut(s) 389, 484, 872, 1418
HpyCH4IV ACGT 2 cut(s) 586, 1281
HpyF10VI GCNNNNNNNGC 7 cut(s) 157, 190, 286, 379, 800, 823, 911
HpyF3I CTNAG 6 cut(s) 334, 428, 790, 830, 1313, 1387
HpySE526I ACGT 2 cut(s) 586, 1281
HspAI GCGC 2 cut(s) 151, 1154
KflI GGGWCCC 1 cut(s) 1382
KspAI GTTAAC 1 cut(s) 697
Kzo9I GATC 7 cut(s) 67, 84, 312, 490, 581, 705, 765
LguI GCTCTTC 1 cut(s) 1164
LmnI GCTCC 1 cut(s) 881
Lsp1109I GCAGC 5 cut(s) 180, 257, 285, 434, 982
LweI GCATC 4 cut(s) 480, 628, 634, 1346
MaeI CTAG 2 cut(s) 731, 975
MaeII ACGT 2 cut(s) 586, 1281
MaeIII GTNAC 2 cut(s) 8, 1080
MalI GATC 7 cut(s) 69, 86, 314, 492, 583, 707, 767
MboI GATC 7 cut(s) 67, 84, 312, 490, 581, 705, 765
MboII GAAGA 8 cut(s) 115, 149, 511, 775, 889, 1154, 1181, 1288
MflI RGATCY 1 cut(s) 705
MhlI GDGCHC 4 cut(s) 656, 870, 1032, 1077
MlsI TGGCCA 1 cut(s) 757
MluNI TGGCCA 1 cut(s) 757
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 918, 961
MnlI CCTC 9 cut(s) 133, 299, 451, 614, 916, 1112, 1139, 1362, 1396
Mox20I TGGCCA 1 cut(s) 757
Mph1103I ATGCAT 2 cut(s) 649, 1228
MroXI GAANNNNTTC 1 cut(s) 1187
MscI TGGCCA 1 cut(s) 757
MseI TTAA 4 cut(s) 264, 696, 1370, 1420
Msp20I TGGCCA 1 cut(s) 757
MspR9I CCNGG 3 cut(s) 402, 1078, 1332
Mva1269I GAATGC 1 cut(s) 1228
MvaI CCWGG 3 cut(s) 402, 1078, 1332
MwoI GCNNNNNNNGC 7 cut(s) 157, 190, 286, 379, 800, 823, 911
NcoI CCATGG 1 cut(s) 709
NdeI CATATG 1 cut(s) 454
NdeII GATC 7 cut(s) 67, 84, 312, 490, 581, 705, 765
NlaIV GGNNCC 6 cut(s) 45, 625, 1074, 1123, 1383, 1384
NsiI ATGCAT 2 cut(s) 649, 1228
NspI RCATGY 2 cut(s) 37, 817
PagI TCATGA 1 cut(s) 720
PciSI GCTCTTC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 1 cut(s) 927
PctI GAATGC 1 cut(s) 1228
PdmI GAANNNNTTC 1 cut(s) 1187
PfeI GAWTC 3 cut(s) 73, 393, 1159
PflFI GACNNNGTC 1 cut(s) 1280
PkrI GCNGC 5 cut(s) 195, 272, 275, 424, 972
PleI GAGTC 1 cut(s) 113
PmaCI CACGTG 1 cut(s) 587
PmlI CACGTG 1 cut(s) 587
PpsI GAGTC 1 cut(s) 113
Ppu21I YACGTR 1 cut(s) 587
PpuMI RGGWCCY 2 cut(s) 1122, 1382
PshBI ATTAAT 1 cut(s) 264
Psp5II RGGWCCY 2 cut(s) 1122, 1382
Psp6I CCWGG 3 cut(s) 400, 1076, 1330
PspCI CACGTG 1 cut(s) 587
PspEI GGTNACC 1 cut(s) 1080
PspFI CCCAGC 1 cut(s) 228
PspGI CCWGG 3 cut(s) 400, 1076, 1330
PspN4I GGNNCC 6 cut(s) 45, 625, 1074, 1123, 1383, 1384
PspPI GGNCC 5 cut(s) 43, 1099, 1122, 1271, 1382
PspPPI RGGWCCY 2 cut(s) 1122, 1382
PstI CTGCAG 2 cut(s) 198, 275
PsuI RGATCY 1 cut(s) 705
PsyI GACNNNGTC 1 cut(s) 1280
RsaI GTAC 3 cut(s) 321, 1268, 1292
RsaNI GTAC 3 cut(s) 320, 1267, 1291
SapI GCTCTTC 1 cut(s) 1164
SaqAI TTAA 4 cut(s) 264, 696, 1370, 1420
SatI GCNGC 5 cut(s) 194, 271, 274, 423, 971
Sau3AI GATC 7 cut(s) 67, 84, 312, 490, 581, 705, 765
Sau96I GGNCC 5 cut(s) 43, 1099, 1122, 1271, 1382
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 3 cut(s) 402, 1078, 1332
SduI GDGCHC 4 cut(s) 656, 870, 1032, 1077
SfaNI GCATC 4 cut(s) 480, 628, 634, 1346
SfcI CTRYAG 3 cut(s) 194, 271, 480
SinI GGWCC 4 cut(s) 43, 1122, 1271, 1382
SmlI CTYRAG 1 cut(s) 1353
SmoI CTYRAG 1 cut(s) 1353
SsiI CCGC 1 cut(s) 879
SspMI CTAG 2 cut(s) 731, 975
StyD4I CCNGG 3 cut(s) 400, 1076, 1330
StyI CCWWGG 2 cut(s) 709, 974
TaaI ACNGT 4 cut(s) 389, 484, 872, 1418
TaiI ACGT 2 cut(s) 589, 1284
TaqI TCGA 4 cut(s) 206, 237, 768, 1284
TfiI GAWTC 3 cut(s) 73, 393, 1159
Tru1I TTAA 4 cut(s) 264, 696, 1370, 1420
Tru9I TTAA 4 cut(s) 264, 696, 1370, 1420
TscAI CASTG 2 cut(s) 436, 500
TseI GCWGC 5 cut(s) 193, 270, 273, 422, 970
TspDTI ATGAA 5 cut(s) 693, 709, 737, 1029, 1164
TspGWI ACGGA 2 cut(s) 714, 1284
TspRI CASTG 2 cut(s) 436, 500
Tth111I GACNNNGTC 1 cut(s) 1280
VpaK11BI GGWCC 4 cut(s) 43, 1122, 1271, 1382
VspI ATTAAT 1 cut(s) 264
XapI RAATTY 3 cut(s) 361, 992, 1366
XceI RCATGY 2 cut(s) 37, 817
XmaJI CCTAGG 1 cut(s) 974
XmnI GAANNNNTTC 1 cut(s) 1187
XspI CTAG 2 cut(s) 731, 975
Zsp2I ATGCAT 2 cut(s) 649, 1228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.