Rh2CG185700

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003099
Physical Location & Seq
Reverse (-)
17252923 .. 17255790
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG185700.1

Sequence Viewer

Length: 1293 bp
ATGGCTTGTAACAAGAGTTTTTCCAGCAATTACATGCTACTGGACCCCAACGATAAGTTGCTGCAGTTTGTGTCGAAGCCAATGGCAGTTATTGGCTGGGCAATCGAAATGTTCTTGAACTTTTTGTCAATTAATGGCTGCAGCTTGCATGGACTGCTTCTAAATCTTTTGCGAGGCAAGATCATAGTACCAGATAGCAACTCAGAAACATTCTTGTCGTTTATTGGGAATTTGGATAAGCGAGTAAAGCTGGACAGTAGAATCAAACCTGGAGATGCCAAATATAATGCAGCACTCAGTGTGATGGCCTCTAAACTATCATATGAGAACCATGCCTGCATCAAAACTACAGTCCAAGATCACTGGAAGATGGAGTTCGTGGGGTTTTACGACTTCTGGAATGAGTATCAGGGAAAAGCTACAACACAAGCCTTTATGCTTCGCGACAGATCACGTGCTGATCATGATACCATTGTTGTTGCCTTTAGAGGAACCGAACCCTTTGATGCAGATGCATGGTGCTCTGACTTTGATATTTCGTGGTATGAACTTGAAGGTGGAGTTAACGGAAAGATCCATGGTGGCTTCATGAAAGCTCTAGGTTTACAGAAAAATATCGGTTGGCCAAAGAAGATCCAGCAAGATGACAAGCACCCCTTAGCTTACTATGCTGTTAGGGACATGCTGAAAAAGCTACTAAGTGAAAATGATAAAGCGAGATATATCATAACAGGGCACAGTTTAGGCGGAGCGTTAGCAATTCTCTTCCCTGCAATTTTGGCATTGCACGAGGAAAACGACCTGTTGGAGAAATTGGAAGGAGTTTATACATTTGGGCAGCCTAGGGTTGGAGACAAGGAATTTGGGCAATACATGGAAAATGAAGTATTGAAAGAGCACAATGTTAGGTATTTTAGGTTTGTTTATAGTAATGATATGGTGCCCAGGTTACCCTATGATGACAAGGCCCTCATGTTCAAGCACTTTGGGACCTGCCTCTACTATAACAGAAACTATGAAGCGCAGATTCTCCCAGAAGAGCCAAACAAGAATTACTTCTCCCCATCGGTGCAAATAACTATGATGGTGAATGCATTTTATGAGCTGACGAGAAGCTTCACAATTCCAAATAAGTACGGACCTGACTACGTCGAAGGGTACTTCTTCAGATTGTTTAGGCTAAGTGGTTTGCTAATGCCAGGGGCATCAGCACACTGTCCCCAAGATTACGTGAATTTAACCAGATTGGGACCCTCAGATGTGTTCCTCTCACCTAAGCCCAAGCAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

430

Amino Acids

49.28

Weight (kDa)

6.83

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 159 - 320 1.2e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1001
AccB1I GGYRCC 1 cut(s) 940
AccII CGCG 1 cut(s) 444
AciI CCGC 1 cut(s) 747
AclWI GGATC 2 cut(s) 568, 628
AcoI YGGCCR 1 cut(s) 623
AcsI RAATTY 3 cut(s) 229, 860, 1234
AcuI CTGAAG 1 cut(s) 1150
AcvI CACGTG 1 cut(s) 455
AdeI CACNNNGTG 1 cut(s) 299
AfaI GTAC 3 cut(s) 189, 1136, 1160
AfiI CCNNNNNNNGG 1 cut(s) 848
AgsI TTSAA 4 cut(s) 118, 554, 892, 979
AjnI CCWGG 3 cut(s) 268, 944, 1198
AluBI AGCT 8 cut(s) 144, 250, 419, 596, 662, 694, 1105, 1116
AluI AGCT 8 cut(s) 144, 250, 419, 596, 662, 694, 1105, 1116
Alw21I GWGCWC 2 cut(s) 524, 900
Alw26I GTCTC 1 cut(s) 846
AlwI GGATC 2 cut(s) 568, 628
AoxI GGCC 3 cut(s) 306, 623, 966
ApeKI GCWGC 5 cut(s) 61, 138, 141, 290, 838
ApoI RAATTY 3 cut(s) 229, 860, 1234
ArsI GACNNNNNNTTYG 4 cut(s) 336, 368, 845, 877
AseI ATTAAT 1 cut(s) 132
Asp700I GAANNNNTTC 1 cut(s) 1055
AspA2I CCTAGG 1 cut(s) 842
AspLEI GCGC 1 cut(s) 1024
AspS9I GGNCC 5 cut(s) 43, 967, 990, 1139, 1250
AsuHPI GGTGA 2 cut(s) 1099, 1263
AvaII GGWCC 4 cut(s) 43, 990, 1139, 1250
AvrII CCTAGG 1 cut(s) 842
BaeGI GKGCMC 2 cut(s) 738, 945
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BalI TGGCCA 1 cut(s) 625
BanI GGYRCC 1 cut(s) 940
BauI CACGAG 1 cut(s) 788
BbrPI CACGTG 1 cut(s) 455
Bbv12I GWGCWC 2 cut(s) 524, 900
BbvI GCAGC 5 cut(s) 48, 125, 153, 302, 850
BccI CCATC 4 cut(s) 298, 364, 1072, 1078
BciT130I CCWGG 3 cut(s) 270, 946, 1200
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 846
BfaI CTAG 2 cut(s) 599, 843
BfmI CTRYAG 3 cut(s) 62, 139, 348
BfuAI ACCTGC 1 cut(s) 1001
BisI GCNGC 5 cut(s) 62, 139, 142, 291, 839
BlnI CCTAGG 1 cut(s) 842
BlsI GCNGC 5 cut(s) 63, 140, 143, 292, 840
Bme1390I CCNGG 3 cut(s) 270, 946, 1200
Bme18I GGWCC 4 cut(s) 43, 990, 1139, 1250
BmgT120I GGNCC 5 cut(s) 43, 967, 990, 1139, 1250
BmiI GGNNCC 6 cut(s) 45, 493, 942, 991, 1251, 1252
BmrFI CCNGG 3 cut(s) 270, 946, 1200
BmsI GCATC 5 cut(s) 265, 348, 496, 502, 1214
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 2 cut(s) 658, 1275
BsaAI YACGTR 2 cut(s) 455, 1231
BsaJI CCNNGG 4 cut(s) 577, 842, 944, 1199
Bsc4I CCNNNNNNNGG 1 cut(s) 848
Bse1I ACTGG 2 cut(s) 45, 368
Bse3DI GCAATG 1 cut(s) 782
BseBI CCWGG 3 cut(s) 270, 946, 1200
BseDI CCNNGG 4 cut(s) 577, 842, 944, 1199
BseLI CCNNNNNNNGG 1 cut(s) 848
BseMI GCAATG 1 cut(s) 782
BseMII CTCAG 3 cut(s) 216, 310, 1269
BseNI ACTGG 2 cut(s) 45, 368
BseSI GKGCMC 2 cut(s) 738, 945
BseXI GCAGC 5 cut(s) 48, 125, 153, 302, 850
BseYI CCCAGC 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 3 cut(s) 308, 625, 968
BshNI GGYRCC 1 cut(s) 940
BsiHKAI GWGCWC 2 cut(s) 524, 900
BslFI GGGAC 4 cut(s) 692, 1003, 1203, 1263
BslI CCNNNNNNNGG 1 cut(s) 848
BsmAI GTCTC 1 cut(s) 846
BsmFI GGGAC 4 cut(s) 692, 1003, 1203, 1263
BsmI GAATGC 1 cut(s) 1096
BsnI GGCC 3 cut(s) 308, 625, 968
Bsp1286I GDGCHC 4 cut(s) 524, 738, 900, 945
Bsp143I GATC 6 cut(s) 180, 358, 449, 460, 573, 633
Bsp19I CCATGG 1 cut(s) 577
Bsp68I TCGCGA 1 cut(s) 444
BspACI CCGC 1 cut(s) 747
BspANI GGCC 3 cut(s) 308, 625, 968
BspCNI CTCAG 3 cut(s) 215, 309, 1268
BspFNI CGCG 1 cut(s) 444
BspHI TCATGA 2 cut(s) 463, 588
BspLI GGNNCC 6 cut(s) 45, 493, 942, 991, 1251, 1252
BspMAI CTGCAG 2 cut(s) 66, 143
BspMI ACCTGC 1 cut(s) 1001
BspPI GGATC 2 cut(s) 568, 628
BspQI GCTCTTC 1 cut(s) 1032
BspT107I GGYRCC 1 cut(s) 940
BsrDI GCAATG 1 cut(s) 782
BsrI ACTGG 2 cut(s) 45, 368
BssECI CCNNGG 4 cut(s) 577, 842, 944, 1199
BssMI GATC 6 cut(s) 180, 358, 449, 460, 573, 633
BssSI CACGAG 1 cut(s) 788
BssT1I CCWWGG 2 cut(s) 577, 842
Bst2BI CACGAG 1 cut(s) 788
Bst2UI CCWGG 3 cut(s) 270, 946, 1200
Bst4CI ACNGT 4 cut(s) 257, 352, 740, 1217
Bst6I CTCTTC 2 cut(s) 770, 1032
BstAPI GCANNNNNTGC 1 cut(s) 154
BstBAI YACGTR 2 cut(s) 455, 1231
BstC8I GCNNGC 2 cut(s) 146, 337
BstDEI CTNAG 7 cut(s) 202, 296, 658, 698, 1181, 1255, 1275
BstDSI CCRYGG 1 cut(s) 577
BstEII GGTNACC 1 cut(s) 948
BstFNI CGCG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 1024
BstKTI GATC 6 cut(s) 183, 361, 452, 463, 576, 636
BstMAI GTCTC 1 cut(s) 846
BstMBI GATC 6 cut(s) 180, 358, 449, 460, 573, 633
BstMWI GCNNNNNNNGC 5 cut(s) 154, 247, 668, 691, 779
BstNI CCWGG 3 cut(s) 270, 946, 1200
BstNSI RCATGY 2 cut(s) 37, 685
BstPI GGTNACC 1 cut(s) 948
BstSCI CCNGG 3 cut(s) 268, 944, 1198
BstSFI CTRYAG 3 cut(s) 62, 139, 348
BstSLI GKGCMC 2 cut(s) 738, 945
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 5 cut(s) 48, 125, 153, 302, 850
BstX2I RGATCY 2 cut(s) 573, 633
BstYI RGATCY 2 cut(s) 573, 633
BsuRI GGCC 3 cut(s) 308, 625, 968
BtgI CCRYGG 1 cut(s) 577
BtsIMutI CAGTG 3 cut(s) 304, 361, 1213
BtuMI TCGCGA 1 cut(s) 444
BveI ACCTGC 1 cut(s) 1001
Cac8I GCNNGC 2 cut(s) 146, 337
CciI TCATGA 2 cut(s) 463, 588
CfoI GCGC 1 cut(s) 1024
Cfr13I GGNCC 5 cut(s) 43, 967, 990, 1139, 1250
Csp6I GTAC 3 cut(s) 188, 1135, 1159
CviQI GTAC 3 cut(s) 188, 1135, 1159
DdeI CTNAG 7 cut(s) 202, 296, 658, 698, 1181, 1255, 1275
DpnI GATC 6 cut(s) 182, 360, 451, 462, 575, 635
DpnII GATC 6 cut(s) 180, 358, 449, 460, 573, 633
DraIII CACNNNGTG 1 cut(s) 299
EaeI YGGCCR 1 cut(s) 623
Eam1104I CTCTTC 2 cut(s) 770, 1032
EarI CTCTTC 2 cut(s) 770, 1032
EciI GGCGGA 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 577, 842
Eco47I GGWCC 4 cut(s) 43, 990, 1139, 1250
Eco57I CTGAAG 1 cut(s) 1150
Eco72I CACGTG 1 cut(s) 455
Eco91I GGTNACC 1 cut(s) 948
EcoO109I RGGNCCY 3 cut(s) 967, 990, 1250
EcoO65I GGTNACC 1 cut(s) 948
EcoRII CCWGG 3 cut(s) 268, 944, 1198
EcoT14I CCWWGG 2 cut(s) 577, 842
EcoT22I ATGCAT 2 cut(s) 517, 1096
ErhI CCWWGG 2 cut(s) 577, 842
FalI AAGNNNNNCTT 4 cut(s) 641, 673, 1040, 1072
FaqI GGGAC 4 cut(s) 692, 1003, 1203, 1263
FauNDI CATATG 1 cut(s) 322
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 5 cut(s) 62, 139, 142, 291, 839
Fsp4HI GCNGC 5 cut(s) 62, 139, 142, 291, 839
FspBI CTAG 2 cut(s) 599, 843
GlaI GCGC 1 cut(s) 1023
GluI GCNGC 5 cut(s) 62, 139, 142, 291, 839
GsaI CCCAGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 3 cut(s) 308, 625, 968
HhaI GCGC 1 cut(s) 1024
Hin6I GCGC 1 cut(s) 1022
HinP1I GCGC 1 cut(s) 1022
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 1114
HinfI GANTC 2 cut(s) 261, 1027
HpaI GTTAAC 1 cut(s) 565
HphI GGTGA 2 cut(s) 1099, 1263
Hpy166II GTNNAC 2 cut(s) 565, 605
Hpy188I TCNGA 4 cut(s) 205, 526, 1169, 1258
Hpy188III TCNNGA 5 cut(s) 115, 397, 443, 464, 589
Hpy8I GTNNAC 2 cut(s) 565, 605
Hpy99I CGWCG 1 cut(s) 1154
HpyAV CCTTC 3 cut(s) 548, 812, 1148
HpyCH4III ACNGT 4 cut(s) 257, 352, 740, 1217
HpyCH4IV ACGT 3 cut(s) 454, 1149, 1230
HpyF10VI GCNNNNNNNGC 5 cut(s) 154, 247, 668, 691, 779
HpyF3I CTNAG 7 cut(s) 202, 296, 658, 698, 1181, 1255, 1275
HpySE526I ACGT 3 cut(s) 454, 1149, 1230
HspAI GCGC 1 cut(s) 1022
KflI GGGWCCC 1 cut(s) 1250
Ksp22I TGATCA 1 cut(s) 460
KspAI GTTAAC 1 cut(s) 565
Kzo9I GATC 6 cut(s) 180, 358, 449, 460, 573, 633
LguI GCTCTTC 1 cut(s) 1032
LmnI GCTCC 1 cut(s) 749
Lsp1109I GCAGC 5 cut(s) 48, 125, 153, 302, 850
LweI GCATC 5 cut(s) 265, 348, 496, 502, 1214
MaeI CTAG 2 cut(s) 599, 843
MaeII ACGT 3 cut(s) 454, 1149, 1230
MaeIII GTNAC 2 cut(s) 8, 948
MalI GATC 6 cut(s) 182, 360, 451, 462, 575, 635
MboI GATC 6 cut(s) 180, 358, 449, 460, 573, 633
MboII GAAGA 5 cut(s) 379, 643, 757, 1049, 1156
MfeI CAATTG 1 cut(s) 1286
MflI RGATCY 2 cut(s) 573, 633
MhlI GDGCHC 4 cut(s) 524, 738, 900, 945
MlsI TGGCCA 1 cut(s) 625
MluNI TGGCCA 1 cut(s) 625
MmeI TCCRAC 2 cut(s) 786, 829
MnlI CCTC 8 cut(s) 167, 319, 482, 784, 980, 1007, 1264, 1277
Mox20I TGGCCA 1 cut(s) 625
Mph1103I ATGCAT 2 cut(s) 517, 1096
MroXI GAANNNNTTC 1 cut(s) 1055
MscI TGGCCA 1 cut(s) 625
MseI TTAA 3 cut(s) 132, 564, 1238
Msp20I TGGCCA 1 cut(s) 625
MspR9I CCNGG 3 cut(s) 270, 946, 1200
MunI CAATTG 1 cut(s) 1286
Mva1269I GAATGC 1 cut(s) 1096
MvaI CCWGG 3 cut(s) 270, 946, 1200
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 5 cut(s) 154, 247, 668, 691, 779
NcoI CCATGG 1 cut(s) 577
NdeI CATATG 1 cut(s) 322
NdeII GATC 6 cut(s) 180, 358, 449, 460, 573, 633
NlaIV GGNNCC 6 cut(s) 45, 493, 942, 991, 1251, 1252
NruI TCGCGA 1 cut(s) 444
NsiI ATGCAT 2 cut(s) 517, 1096
NspI RCATGY 2 cut(s) 37, 685
PagI TCATGA 2 cut(s) 463, 588
PciSI GCTCTTC 1 cut(s) 1032
PcsI WCGNNNNNNNCGW 1 cut(s) 795
PctI GAATGC 1 cut(s) 1096
PdmI GAANNNNTTC 1 cut(s) 1055
PfeI GAWTC 2 cut(s) 261, 1027
PflFI GACNNNGTC 1 cut(s) 1148
PkrI GCNGC 5 cut(s) 63, 140, 143, 292, 840
PmaCI CACGTG 1 cut(s) 455
PmlI CACGTG 1 cut(s) 455
Ppu21I YACGTR 2 cut(s) 455, 1231
PpuMI RGGWCCY 2 cut(s) 990, 1250
PshBI ATTAAT 1 cut(s) 132
Psp5II RGGWCCY 2 cut(s) 990, 1250
Psp6I CCWGG 3 cut(s) 268, 944, 1198
PspCI CACGTG 1 cut(s) 455
PspEI GGTNACC 1 cut(s) 948
PspFI CCCAGC 1 cut(s) 96
PspGI CCWGG 3 cut(s) 268, 944, 1198
PspN4I GGNNCC 6 cut(s) 45, 493, 942, 991, 1251, 1252
PspPI GGNCC 5 cut(s) 43, 967, 990, 1139, 1250
PspPPI RGGWCCY 2 cut(s) 990, 1250
PstI CTGCAG 2 cut(s) 66, 143
PsuI RGATCY 2 cut(s) 573, 633
PsyI GACNNNGTC 1 cut(s) 1148
RruI TCGCGA 1 cut(s) 444
RsaI GTAC 3 cut(s) 189, 1136, 1160
RsaNI GTAC 3 cut(s) 188, 1135, 1159
SapI GCTCTTC 1 cut(s) 1032
SaqAI TTAA 3 cut(s) 132, 564, 1238
SatI GCNGC 5 cut(s) 62, 139, 142, 291, 839
Sau3AI GATC 6 cut(s) 180, 358, 449, 460, 573, 633
Sau96I GGNCC 5 cut(s) 43, 967, 990, 1139, 1250
ScrFI CCNGG 3 cut(s) 270, 946, 1200
SduI GDGCHC 4 cut(s) 524, 738, 900, 945
SfaNI GCATC 5 cut(s) 265, 348, 496, 502, 1214
SfcI CTRYAG 3 cut(s) 62, 139, 348
SinI GGWCC 4 cut(s) 43, 990, 1139, 1250
SsiI CCGC 1 cut(s) 747
SspMI CTAG 2 cut(s) 599, 843
StyD4I CCNGG 3 cut(s) 268, 944, 1198
StyI CCWWGG 2 cut(s) 577, 842
TaaI ACNGT 4 cut(s) 257, 352, 740, 1217
TaiI ACGT 3 cut(s) 457, 1152, 1233
TaqI TCGA 3 cut(s) 74, 105, 1152
TfiI GAWTC 2 cut(s) 261, 1027
Tru1I TTAA 3 cut(s) 132, 564, 1238
Tru9I TTAA 3 cut(s) 132, 564, 1238
TscAI CASTG 3 cut(s) 304, 368, 1220
TseI GCWGC 5 cut(s) 61, 138, 141, 290, 838
TspDTI ATGAA 5 cut(s) 561, 577, 605, 897, 1032
TspGWI ACGGA 2 cut(s) 582, 1152
TspRI CASTG 3 cut(s) 304, 368, 1220
Tth111I GACNNNGTC 1 cut(s) 1148
VpaK11BI GGWCC 4 cut(s) 43, 990, 1139, 1250
VspI ATTAAT 1 cut(s) 132
XapI RAATTY 3 cut(s) 229, 860, 1234
XceI RCATGY 2 cut(s) 37, 685
XmaJI CCTAGG 1 cut(s) 842
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 2 cut(s) 599, 843
Zsp2I ATGCAT 2 cut(s) 517, 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.