Rh2DG187400

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003099
Physical Location & Seq
Reverse (-)
16733632 .. 16738387
4756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG187400.1

Sequence Viewer

Length: 1203 bp
ATGGCCTGCGACAAGGGATTTTCCAGCAGTTTTATGCTGCTGAAGCCAGAAGAAGTCGGATTGTTTGGTCTGTTTCACATTTTGTTCTCCAGCAAGTTAGAGGAAAAGAAATTTGTAGATTGCCCGGAGGGGACTGTCACTGATGAGATCAGCTTCAGGCGCAGATGGCTCATATTTGTTTCCATCTTGGCTCAAAAGTTTCTGCAACTTGTGTCAAAGCCACTCTCAGTTGTCGGATCTGGGATCGAAATGTGTTTGAATCTTGTATCCGTCAACCGCAATGTTTTTGTGCTTGTGATCAATTTCTTCCGAGGGGTGGTGGTAAAGCCAGATAAGACATCAGCAACATTCCTGTCATTTATAGGAAATTTTGACAAGAGGGTGGAGTTGGATAGCACCATTAAATATGATGATGCCAAGTACCATGCAGCTCTCTCTATGATGGCTTCTAAAGCATCCTATGAGAATAATGCCTACATTGAAACTACTGTTAGGGATCATTGGGAGATGGAGTTCTTAGGATCTTTCGACTTCTGGAATGATTATCAACAAAAAGCCACAACACAAGCCTTTATGCTTCGGGATAAGTCTTCTGACCATGACATCGTTGTTGTGGCCTTTAGGGGAACCGAACCGTTTGATGCAGATGCATGGTGCTCCGACTTTGACATCTCTTGGTATGAGCTTTCAGGGGTCGGAAAAATTCATGGCGGCTTTATGAAAGCTCTAGGTTTACAAAAGAATGTTGGTTGGCCTAAGGAAACCAAACAAGAAGATGGTCATCCTGACTTGGCTTACTATGCAATTAGAGACATGCTAAACAAGCTTTTGAGCCAAAATGATCAAGCTAGATACATATTGACAGGGCATAGTTTGGGAGGGGCTTTAGCAATTTTGTTTCCAGCAGTTTTGGCTCTCCACAATGAGACATGGTTATTGGAGAGATTGCAAGGGGTATATACATTTGGGCAACCAAGGGTAGGAGATGAAAAGTTTGGTGAGTTCATGGAGAAACAGTTGCAAGAGCATAACTTTCAGTATTTTAGATTTGTTTATGGTAATGATATGGTTCCTAGGTTGCCTTATGATGACAAAGCCTTGATGTTCAAGCACTTTGGGGCATGCCTCTATTATAATAGGCACTATGAAGTGAAGGTAATTAAGTACACTAACCATGCATCCCGATTTATATTTACAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

400

Amino Acids

46.07

Weight (kDa)

6.19

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 204 - 363 4.2e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1134
AciI CCGC 2 cut(s) 277, 711
AclWI GGATC 4 cut(s) 244, 251, 504, 529
AcsI RAATTY 3 cut(s) 110, 367, 702
AcuI CTGAAG 2 cut(s) 62, 139
AfaI GTAC 2 cut(s) 422, 1166
AfiI CCNNNNNNNGG 2 cut(s) 316, 980
AgsI TTSAA 3 cut(s) 259, 482, 1108
AloI GAACNNNNNNTCC 2 cut(s) 497, 529
AluBI AGCT 6 cut(s) 153, 431, 685, 725, 826, 848
AluI AGCT 6 cut(s) 153, 431, 685, 725, 826, 848
Alw21I GWGCWC 1 cut(s) 659
Alw26I GTCTC 2 cut(s) 804, 920
AlwI GGATC 4 cut(s) 244, 251, 504, 529
AoxI GGCC 3 cut(s) 3, 615, 752
ApeKI GCWGC 2 cut(s) 37, 428
ApoI RAATTY 3 cut(s) 110, 367, 702
AspA2I CCTAGG 1 cut(s) 1073
AspLEI GCGC 1 cut(s) 162
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 1010
AvrII CCTAGG 1 cut(s) 1073
AxyI CCTNAGG 1 cut(s) 756
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 659
BbvI GCAGC 2 cut(s) 24, 440
BccI CCATC 5 cut(s) 159, 191, 436, 502, 770
BciVI GTATCC 1 cut(s) 277
BclI TGATCA 2 cut(s) 297, 841
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 804, 920
BfaI CTAG 3 cut(s) 728, 849, 1074
BfuI GTATCC 1 cut(s) 277
BisI GCNGC 3 cut(s) 38, 429, 712
BlnI CCTAGG 1 cut(s) 1073
BlsI GCNGC 3 cut(s) 39, 430, 713
Bme1390I CCNGG 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 628, 1071
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 5 cut(s) 403, 464, 631, 637, 1187
BpiI GAAGAC 1 cut(s) 582
BpmI CTGGAG 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 310, 974, 1073
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 1001, 1031
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 980
Bse21I CCTNAGG 1 cut(s) 756
Bse3DI GCAATG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 780
BseDI CCNNGG 3 cut(s) 310, 974, 1073
BseGI GGATG 3 cut(s) 455, 781, 1178
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 2 cut(s) 316, 980
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 1 cut(s) 240
BseXI GCAGC 2 cut(s) 24, 440
BshFI GGCC 3 cut(s) 5, 617, 754
BsiHKAI GWGCWC 1 cut(s) 659
BsiSI CCGG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 2 cut(s) 316, 980
BsmAI GTCTC 2 cut(s) 804, 920
BsmFI GGGAC 1 cut(s) 145
BsnI GGCC 3 cut(s) 5, 617, 754
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
BspACI CCGC 2 cut(s) 277, 711
BspANI GGCC 3 cut(s) 5, 617, 754
BspCNI CTCAG 1 cut(s) 239
BspLI GGNNCC 2 cut(s) 628, 1071
BspPI GGATC 4 cut(s) 244, 251, 504, 529
BsrDI GCAATG 1 cut(s) 286
BssECI CCNNGG 3 cut(s) 310, 974, 1073
BssMI GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
BssT1I CCWWGG 2 cut(s) 974, 1073
Bst4CI ACNGT 4 cut(s) 136, 490, 636, 1017
BstC8I GCNNGC 2 cut(s) 7, 1123
BstDEI CTNAG 3 cut(s) 226, 517, 756
BstF5I GGATG 3 cut(s) 455, 781, 1178
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 7 cut(s) 150, 239, 246, 300, 499, 524, 844
BstMAI GTCTC 2 cut(s) 804, 920
BstMBI GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
BstMWI GCNNNNNNNGC 7 cut(s) 43, 159, 166, 452, 800, 823, 911
BstNSI RCATGY 2 cut(s) 817, 1125
BstSCI CCNGG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 24, 440
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 2 cut(s) 236, 521
BstYI RGATCY 2 cut(s) 236, 521
Bsu36I CCTNAGG 1 cut(s) 756
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 3 cut(s) 5, 617, 754
BtsCI GGATG 3 cut(s) 455, 781, 1178
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 7, 1123
CfoI GCGC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 421, 1165
CviAII CATG 9 cut(s) 425, 599, 651, 707, 814, 930, 1006, 1122, 1175
CviQI GTAC 2 cut(s) 421, 1165
DdeI CTNAG 3 cut(s) 226, 517, 756
DpnI GATC 7 cut(s) 149, 238, 245, 299, 498, 523, 843
DpnII GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
Eco130I CCWWGG 2 cut(s) 974, 1073
Eco57I CTGAAG 2 cut(s) 62, 139
Eco81I CCTNAGG 1 cut(s) 756
EcoT14I CCWWGG 2 cut(s) 974, 1073
EcoT22I ATGCAT 2 cut(s) 652, 1180
ErhI CCWWGG 2 cut(s) 974, 1073
FaeI CATG 9 cut(s) 428, 602, 654, 710, 817, 933, 1009, 1125, 1178
FaqI GGGAC 1 cut(s) 145
FatI CATG 9 cut(s) 424, 598, 650, 706, 813, 929, 1005, 1121, 1174
FbaI TGATCA 2 cut(s) 297, 841
Fnu4HI GCNGC 3 cut(s) 38, 429, 712
FokI GGATG 3 cut(s) 442, 768, 1165
Fsp4HI GCNGC 3 cut(s) 38, 429, 712
FspBI CTAG 3 cut(s) 728, 849, 1074
GlaI GCGC 1 cut(s) 161
GluI GCNGC 3 cut(s) 38, 429, 712
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 3 cut(s) 5, 617, 754
HapII CCGG 1 cut(s) 125
HhaI GCGC 1 cut(s) 162
Hin1II CATG 9 cut(s) 428, 602, 654, 710, 817, 933, 1009, 1125, 1178
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 1 cut(s) 259
HpaII CCGG 1 cut(s) 125
HphI GGTGA 1 cut(s) 1010
Hpy166II GTNNAC 3 cut(s) 274, 734, 1167
Hpy188I TCNGA 6 cut(s) 59, 236, 311, 595, 661, 698
Hpy188III TCNNGA 4 cut(s) 535, 581, 785, 1182
Hpy8I GTNNAC 3 cut(s) 274, 734, 1167
HpyAV CCTTC 1 cut(s) 1147
HpyCH4III ACNGT 4 cut(s) 136, 490, 636, 1017
HpyCH4V TGCA 8 cut(s) 205, 428, 644, 650, 803, 949, 1021, 1178
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 159, 166, 452, 800, 823, 911
HpyF3I CTNAG 3 cut(s) 226, 517, 756
Hsp92II CATG 9 cut(s) 428, 602, 654, 710, 817, 933, 1009, 1125, 1178
HspAI GCGC 1 cut(s) 160
Ksp22I TGATCA 2 cut(s) 297, 841
Kzo9I GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
LmnI GCTCC 1 cut(s) 662
Lsp1109I GCAGC 2 cut(s) 24, 440
LweI GCATC 5 cut(s) 403, 464, 631, 637, 1187
MaeI CTAG 3 cut(s) 728, 849, 1074
MaeIII GTNAC 1 cut(s) 136
MalI GATC 7 cut(s) 149, 238, 245, 299, 498, 523, 843
MboI GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
MboII GAAGA 4 cut(s) 62, 298, 582, 785
MflI RGATCY 2 cut(s) 236, 521
MhlI GDGCHC 1 cut(s) 659
MluCI AATT 8 cut(s) 110, 301, 367, 702, 804, 891, 1158, 1197
MmeI TCCRAC 5 cut(s) 37, 214, 369, 676, 684
MnlI CCTC 6 cut(s) 94, 121, 305, 372, 872, 1136
Mph1103I ATGCAT 2 cut(s) 652, 1180
MseI TTAA 2 cut(s) 402, 1161
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 125
MwoI GCNNNNNNNGC 7 cut(s) 43, 159, 166, 452, 800, 823, 911
NciI CCSGG 1 cut(s) 125
NdeII GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
NlaIII CATG 9 cut(s) 428, 602, 654, 710, 817, 933, 1009, 1125, 1178
NlaIV GGNNCC 2 cut(s) 628, 1071
NmuCI GTSAC 1 cut(s) 136
NsiI ATGCAT 2 cut(s) 652, 1180
NspI RCATGY 2 cut(s) 817, 1125
PaeI GCATGC 1 cut(s) 1125
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 3 cut(s) 39, 430, 713
PsiI TTATAA 1 cut(s) 1134
PspN4I GGNNCC 2 cut(s) 628, 1071
PsuI RGATCY 2 cut(s) 236, 521
RsaI GTAC 2 cut(s) 422, 1166
RsaNI GTAC 2 cut(s) 421, 1165
SaqAI TTAA 2 cut(s) 402, 1161
SatI GCNGC 3 cut(s) 38, 429, 712
Sau3AI GATC 7 cut(s) 147, 236, 243, 297, 496, 521, 841
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 659
SetI ASST 9 cut(s) 155, 433, 687, 727, 733, 828, 850, 1079, 1158
SfaNI GCATC 5 cut(s) 403, 464, 631, 637, 1187
SphI GCATGC 1 cut(s) 1125
Sse9I AATT 8 cut(s) 110, 301, 367, 702, 804, 891, 1158, 1197
SsiI CCGC 2 cut(s) 277, 711
SspMI CTAG 3 cut(s) 728, 849, 1074
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 2 cut(s) 974, 1073
TaaI ACNGT 4 cut(s) 136, 490, 636, 1017
TaqI TCGA 2 cut(s) 246, 528
TasI AATT 8 cut(s) 110, 301, 367, 702, 804, 891, 1158, 1197
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 402, 1161
Tru9I TTAA 2 cut(s) 402, 1161
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 2 cut(s) 37, 428
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 5 cut(s) 695, 734, 994, 1002, 1161
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 1 cut(s) 145
XapI RAATTY 3 cut(s) 110, 367, 702
XceI RCATGY 2 cut(s) 817, 1125
XmaJI CCTAGG 1 cut(s) 1073
XspI CTAG 3 cut(s) 728, 849, 1074
Zsp2I ATGCAT 2 cut(s) 652, 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.