Rroxscaffold_2G00137920

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003099
Physical Location & Seq
Forward (+)
75899080 .. 75903141
4062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00137920.1

Sequence Viewer

Length: 1428 bp
ATGGCTTGTAACAAGAGTTTTTCCAGCAGTTACATGCTACTGGACCCCAACGATGTGGGTTTCTTTGATCTGATTCACATCTTGATCTCCAATAACTTGGAGAAGAGAAAGTTCGTTGACTCCTCCGATGACAAAGAAGATGACTTTCAGCGCAGATGGCTCATATTTCTGTCTATTGTGGTGCAGAAGTTGCTGCAGTTTGTGTCGAAGCCAATGGCGGTTATTGGCTGGGCAATCGAAATGTTCTTGAACTTTTTGTCAATTAATGGCTGCAGCTTGCATGGCCTGCTCCTAAATCTTTTGCGAGGCAAGATCATAGTACCAGATAGCAACTCAGAAACATTCTTGTCGTTTATTGGACATTTGGATAAGCGAGTAAAGCTGGACAGTAGAATCAAACCTGGAGATGGCAAATATAATGCAGCACTCAGTGTGATGGCCTCTAAATTATCATATGAGAACCATGCCTGCATCAAAACTACAGTCCAAGATCACTGGAAGATGGAGTTCGTGGGGTTTTATGACTTCTGGAATGAGTATCAAGGAAAAGCTACAACACAAGCCTTTATGCTTCAGGACAGATCAAGTGCTGACCATGATACCATTGTTGTTGCCTTTAGAGGAACCGAACCCTTTGATGCAGATGCATGGTGCTCTGACTTTGATATTTCGTGGTATGAGCTTGAAGGTGGAGTTAACGGAAAGATCCATGGTGGCTTCATGAAAGCTCTAGGTCTACAGAAAAATATCGGTTGGCCAAAGAAAATCGAGCAAGATGACAAGCACCCCTTAGCTTACTATGCTGTTAGGGACATGCTGAAAAAGCGTCTTAGTGAAACTGATAAGGCGAGATATATCATAACGGGGCACAGTTTAGGCGGAGCGTTAGCAATTCTCTTCCCTGCAATTTTGGCATTGCACGAGGAAAACGAGCTGTTGGAGAAATTGGAAGGAGTTTATACATTTGGGCAGCCAAGGGTTGGAGACAAGGAATTTGGGCAATACATGGAAAATGAAGTATTGAAAGAGCACAATGTTAGGTATTTTAGGTTTGTTTATAGTAATGATATGGTGCCTAGGTTACCTTATGATGACAAGGCCCTCATGTTCAAGCACTTTGGGACCTGCCTCTATTATAACAGAAACTATGAAGCACAGATTCTTCCAGAAGAGCCAAACAAGAATTACTTCTCCCCATCGGTGCAAATAACTATGATGGTGAATGCATTTTATGAGCTGACGAGAAGCTTCACAATGCCAAATAAGTACGGACCTGACTACGTCGAAGGGTACTTCTTCAGATTGTTTAGGCTAAGTGGTTTGCTAATGCCAGGGGCATCAGCACATTGTCCCCAAGATTATGTGAATTTAACCAGATTGGGTCGACCCTCAGATGTGTTCGTCTCACCTAAGCCCAAGCAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

475

Amino Acids

54.65

Weight (kDa)

6.37

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 203 - 364 3.4e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1137
Acc36I ACCTGC 1 cut(s) 1133
AccB1I GGYRCC 1 cut(s) 1072
AccB7I CCANNNNNTGG 1 cut(s) 980
AccI GTMKAC 2 cut(s) 736, 1384
AciI CCGC 2 cut(s) 218, 879
AclWI GGATC 1 cut(s) 700
AcoI YGGCCR 1 cut(s) 755
AcsI RAATTY 2 cut(s) 992, 1366
AcuI CTGAAG 2 cut(s) 557, 1282
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 3 cut(s) 321, 1268, 1292
AfiI CCNNNNNNNGG 2 cut(s) 407, 980
AgsI TTSAA 4 cut(s) 250, 686, 1024, 1111
AjnI CCWGG 2 cut(s) 400, 1330
AluBI AGCT 9 cut(s) 276, 382, 551, 682, 728, 794, 934, 1237, 1248
AluI AGCT 9 cut(s) 276, 382, 551, 682, 728, 794, 934, 1237, 1248
Alw21I GWGCWC 2 cut(s) 656, 1032
Alw26I GTCTC 2 cut(s) 978, 1408
AlwI GGATC 1 cut(s) 700
AoxI GGCC 4 cut(s) 283, 438, 755, 1098
ApeKI GCWGC 5 cut(s) 193, 270, 273, 422, 970
ApoI RAATTY 2 cut(s) 992, 1366
ArsI GACNNNNNNTTYG 4 cut(s) 468, 500, 977, 1009
AseI ATTAAT 1 cut(s) 264
Asp700I GAANNNNTTC 1 cut(s) 1187
AspA2I CCTAGG 1 cut(s) 1076
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 4 cut(s) 43, 1099, 1122, 1271
AsuHPI GGTGA 2 cut(s) 1231, 1398
AvaII GGWCC 3 cut(s) 43, 1122, 1271
AvrII CCTAGG 1 cut(s) 1076
BaeGI GKGCMC 1 cut(s) 870
BaeI ACNNNNGTAYC 2 cut(s) 591, 624
BalI TGGCCA 1 cut(s) 757
BanI GGYRCC 1 cut(s) 1072
BauI CACGAG 1 cut(s) 920
Bbv12I GWGCWC 2 cut(s) 656, 1032
BbvI GCAGC 5 cut(s) 180, 257, 285, 434, 982
BccI CCATC 6 cut(s) 150, 401, 430, 496, 1204, 1210
BciT130I CCWGG 2 cut(s) 402, 1332
BcoDI GTCTC 2 cut(s) 978, 1408
BfaI CTAG 2 cut(s) 731, 1077
BfmI CTRYAG 4 cut(s) 194, 271, 480, 737
BfuAI ACCTGC 1 cut(s) 1133
BisI GCNGC 5 cut(s) 194, 271, 274, 423, 971
BlnI CCTAGG 1 cut(s) 1076
BlsI GCNGC 5 cut(s) 195, 272, 275, 424, 972
Bme1390I CCNGG 2 cut(s) 402, 1332
Bme18I GGWCC 3 cut(s) 43, 1122, 1271
BmgT120I GGNCC 4 cut(s) 43, 1099, 1122, 1271
BmiI GGNNCC 4 cut(s) 45, 625, 1074, 1123
BmrFI CCNGG 2 cut(s) 402, 1332
BmsI GCATC 4 cut(s) 480, 628, 634, 1346
BpmI CTGGAG 1 cut(s) 423
Bpu10I CCTNAGC 2 cut(s) 790, 1410
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 709, 974, 1076, 1331
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 980
Bse1I ACTGG 2 cut(s) 45, 500
Bse3DI GCAATG 1 cut(s) 914
Bse8I GATNNNNATC 1 cut(s) 77
BseBI CCWGG 2 cut(s) 402, 1332
BseDI CCNNGG 4 cut(s) 709, 974, 1076, 1331
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 407, 980
BseMI GCAATG 1 cut(s) 914
BseMII CTCAG 3 cut(s) 348, 442, 1404
BseNI ACTGG 2 cut(s) 45, 500
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 870
BseXI GCAGC 5 cut(s) 180, 257, 285, 434, 982
BseYI CCCAGC 1 cut(s) 228
BsgI GTGCAG 1 cut(s) 203
BshFI GGCC 4 cut(s) 285, 440, 757, 1100
BshNI GGYRCC 1 cut(s) 1072
BsiHKAI GWGCWC 2 cut(s) 656, 1032
BslFI GGGAC 3 cut(s) 824, 1135, 1335
BslI CCNNNNNNNGG 2 cut(s) 407, 980
BsmAI GTCTC 2 cut(s) 978, 1408
BsmBI CGTCTC 1 cut(s) 1408
BsmFI GGGAC 3 cut(s) 824, 1135, 1335
BsmI GAATGC 1 cut(s) 1228
BsnI GGCC 4 cut(s) 285, 440, 757, 1100
Bsp1286I GDGCHC 3 cut(s) 656, 870, 1032
Bsp143I GATC 6 cut(s) 67, 84, 312, 490, 581, 705
Bsp19I CCATGG 1 cut(s) 709
BspACI CCGC 2 cut(s) 218, 879
BspANI GGCC 4 cut(s) 285, 440, 757, 1100
BspCNI CTCAG 3 cut(s) 347, 441, 1403
BspHI TCATGA 1 cut(s) 720
BspLI GGNNCC 4 cut(s) 45, 625, 1074, 1123
BspMAI CTGCAG 2 cut(s) 198, 275
BspMI ACCTGC 1 cut(s) 1133
BspPI GGATC 1 cut(s) 700
BspQI GCTCTTC 1 cut(s) 1164
BspT107I GGYRCC 1 cut(s) 1072
BsrDI GCAATG 1 cut(s) 914
BsrI ACTGG 2 cut(s) 45, 500
BssECI CCNNGG 4 cut(s) 709, 974, 1076, 1331
BssMI GATC 6 cut(s) 67, 84, 312, 490, 581, 705
BssSI CACGAG 1 cut(s) 920
BssT1I CCWWGG 3 cut(s) 709, 974, 1076
Bst2BI CACGAG 1 cut(s) 920
Bst2UI CCWGG 2 cut(s) 402, 1332
Bst4CI ACNGT 4 cut(s) 389, 484, 872, 1425
Bst6I CTCTTC 3 cut(s) 98, 902, 1164
BstAPI GCANNNNNTGC 2 cut(s) 190, 286
BstC8I GCNNGC 3 cut(s) 278, 287, 469
BstDEI CTNAG 7 cut(s) 334, 428, 790, 830, 1313, 1390, 1410
BstDSI CCRYGG 1 cut(s) 709
BstEII GGTNACC 1 cut(s) 1080
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 6 cut(s) 70, 87, 315, 493, 584, 708
BstMAI GTCTC 2 cut(s) 978, 1408
BstMBI GATC 6 cut(s) 67, 84, 312, 490, 581, 705
BstMWI GCNNNNNNNGC 8 cut(s) 157, 190, 282, 286, 379, 800, 823, 911
BstNI CCWGG 2 cut(s) 402, 1332
BstNSI RCATGY 2 cut(s) 37, 817
BstPI GGTNACC 1 cut(s) 1080
BstSCI CCNGG 2 cut(s) 400, 1330
BstSFI CTRYAG 4 cut(s) 194, 271, 480, 737
BstSLI GKGCMC 1 cut(s) 870
BstV1I GCAGC 5 cut(s) 180, 257, 285, 434, 982
BstX2I RGATCY 1 cut(s) 705
BstXI CCANNNNNNTGG 2 cut(s) 55, 97
BstYI RGATCY 1 cut(s) 705
BsuRI GGCC 4 cut(s) 285, 440, 757, 1100
BtgI CCRYGG 1 cut(s) 709
BtsIMutI CAGTG 2 cut(s) 436, 493
BveI ACCTGC 1 cut(s) 1133
Cac8I GCNNGC 3 cut(s) 278, 287, 469
CciI TCATGA 1 cut(s) 720
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 4 cut(s) 43, 1099, 1122, 1271
CseI GACGC 1 cut(s) 815
Csp6I GTAC 3 cut(s) 320, 1267, 1291
CviQI GTAC 3 cut(s) 320, 1267, 1291
DdeI CTNAG 7 cut(s) 334, 428, 790, 830, 1313, 1390, 1410
DpnI GATC 6 cut(s) 69, 86, 314, 492, 583, 707
DpnII GATC 6 cut(s) 67, 84, 312, 490, 581, 705
DraIII CACNNNGTG 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 755
Eam1104I CTCTTC 3 cut(s) 98, 902, 1164
EarI CTCTTC 3 cut(s) 98, 902, 1164
EciI GGCGGA 1 cut(s) 894
Eco130I CCWWGG 3 cut(s) 709, 974, 1076
Eco47I GGWCC 3 cut(s) 43, 1122, 1271
Eco57I CTGAAG 2 cut(s) 557, 1282
Eco91I GGTNACC 1 cut(s) 1080
EcoO109I RGGNCCY 2 cut(s) 1099, 1122
EcoO65I GGTNACC 1 cut(s) 1080
EcoRII CCWGG 2 cut(s) 400, 1330
EcoT14I CCWWGG 3 cut(s) 709, 974, 1076
EcoT22I ATGCAT 2 cut(s) 649, 1228
ErhI CCWWGG 3 cut(s) 709, 974, 1076
Esp3I CGTCTC 1 cut(s) 1408
FalI AAGNNNNNCTT 4 cut(s) 773, 805, 1172, 1204
FaqI GGGAC 3 cut(s) 824, 1135, 1335
FauNDI CATATG 1 cut(s) 454
FblI GTMKAC 2 cut(s) 736, 1384
Fnu4HI GCNGC 5 cut(s) 194, 271, 274, 423, 971
Fsp4HI GCNGC 5 cut(s) 194, 271, 274, 423, 971
FspBI CTAG 2 cut(s) 731, 1077
GlaI GCGC 1 cut(s) 152
GluI GCNGC 5 cut(s) 194, 271, 274, 423, 971
GsaI CCCAGC 1 cut(s) 232
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 4 cut(s) 285, 440, 757, 1100
HgaI GACGC 1 cut(s) 815
HhaI GCGC 1 cut(s) 153
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HincII GTYRAC 3 cut(s) 118, 697, 1385
HindII GTYRAC 3 cut(s) 118, 697, 1385
HindIII AAGCTT 1 cut(s) 1246
HinfI GANTC 4 cut(s) 73, 119, 393, 1159
HpaI GTTAAC 1 cut(s) 697
HphI GGTGA 2 cut(s) 1231, 1398
Hpy166II GTNNAC 4 cut(s) 118, 697, 737, 1385
Hpy188I TCNGA 6 cut(s) 72, 127, 337, 658, 1301, 1393
Hpy188III TCNNGA 6 cut(s) 82, 247, 529, 575, 721, 1166
Hpy8I GTNNAC 4 cut(s) 118, 697, 737, 1385
Hpy99I CGWCG 1 cut(s) 1286
HpyAV CCTTC 3 cut(s) 680, 944, 1280
HpyCH4III ACNGT 4 cut(s) 389, 484, 872, 1425
HpyCH4IV ACGT 1 cut(s) 1281
HpyF10VI GCNNNNNNNGC 8 cut(s) 157, 190, 282, 286, 379, 800, 823, 911
HpyF3I CTNAG 7 cut(s) 334, 428, 790, 830, 1313, 1390, 1410
HpySE526I ACGT 1 cut(s) 1281
HspAI GCGC 1 cut(s) 151
KspAI GTTAAC 1 cut(s) 697
Kzo9I GATC 6 cut(s) 67, 84, 312, 490, 581, 705
LguI GCTCTTC 1 cut(s) 1164
LmnI GCTCC 2 cut(s) 294, 881
Lsp1109I GCAGC 5 cut(s) 180, 257, 285, 434, 982
LweI GCATC 4 cut(s) 480, 628, 634, 1346
MaeI CTAG 2 cut(s) 731, 1077
MaeII ACGT 1 cut(s) 1281
MaeIII GTNAC 3 cut(s) 8, 29, 1080
MalI GATC 6 cut(s) 69, 86, 314, 492, 583, 707
MboI GATC 6 cut(s) 67, 84, 312, 490, 581, 705
MboII GAAGA 7 cut(s) 115, 149, 511, 889, 1154, 1181, 1288
MflI RGATCY 1 cut(s) 705
MhlI GDGCHC 3 cut(s) 656, 870, 1032
MlsI TGGCCA 1 cut(s) 757
MluCI AATT 8 cut(s) 261, 446, 891, 906, 944, 992, 1183, 1366
MluNI TGGCCA 1 cut(s) 757
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 918, 961
MnlI CCTC 8 cut(s) 133, 299, 451, 614, 916, 1112, 1139, 1399
Mox20I TGGCCA 1 cut(s) 757
Mph1103I ATGCAT 2 cut(s) 649, 1228
MroXI GAANNNNTTC 1 cut(s) 1187
MscI TGGCCA 1 cut(s) 757
MseI TTAA 3 cut(s) 264, 696, 1370
Msp20I TGGCCA 1 cut(s) 757
MspR9I CCNGG 2 cut(s) 402, 1332
Mva1269I GAATGC 1 cut(s) 1228
MvaI CCWGG 2 cut(s) 402, 1332
MwoI GCNNNNNNNGC 8 cut(s) 157, 190, 282, 286, 379, 800, 823, 911
NcoI CCATGG 1 cut(s) 709
NdeI CATATG 1 cut(s) 454
NdeII GATC 6 cut(s) 67, 84, 312, 490, 581, 705
NlaIV GGNNCC 4 cut(s) 45, 625, 1074, 1123
NsiI ATGCAT 2 cut(s) 649, 1228
NspI RCATGY 2 cut(s) 37, 817
PagI TCATGA 1 cut(s) 720
PciSI GCTCTTC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 1 cut(s) 927
PctI GAATGC 1 cut(s) 1228
PdmI GAANNNNTTC 1 cut(s) 1187
PfeI GAWTC 3 cut(s) 73, 393, 1159
PflFI GACNNNGTC 1 cut(s) 1280
PflMI CCANNNNNTGG 1 cut(s) 980
PkrI GCNGC 5 cut(s) 195, 272, 275, 424, 972
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 1122
PshBI ATTAAT 1 cut(s) 264
PsiI TTATAA 1 cut(s) 1137
Psp5II RGGWCCY 1 cut(s) 1122
Psp6I CCWGG 2 cut(s) 400, 1330
PspEI GGTNACC 1 cut(s) 1080
PspFI CCCAGC 1 cut(s) 228
PspGI CCWGG 2 cut(s) 400, 1330
PspN4I GGNNCC 4 cut(s) 45, 625, 1074, 1123
PspPI GGNCC 4 cut(s) 43, 1099, 1122, 1271
PspPPI RGGWCCY 1 cut(s) 1122
PstI CTGCAG 2 cut(s) 198, 275
PsuI RGATCY 1 cut(s) 705
PsyI GACNNNGTC 1 cut(s) 1280
RsaI GTAC 3 cut(s) 321, 1268, 1292
RsaNI GTAC 3 cut(s) 320, 1267, 1291
SalI GTCGAC 1 cut(s) 1383
SapI GCTCTTC 1 cut(s) 1164
SaqAI TTAA 3 cut(s) 264, 696, 1370
SatI GCNGC 5 cut(s) 194, 271, 274, 423, 971
Sau3AI GATC 6 cut(s) 67, 84, 312, 490, 581, 705
Sau96I GGNCC 4 cut(s) 43, 1099, 1122, 1271
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 2 cut(s) 402, 1332
SduI GDGCHC 3 cut(s) 656, 870, 1032
SfaNI GCATC 4 cut(s) 480, 628, 634, 1346
SfcI CTRYAG 4 cut(s) 194, 271, 480, 737
SinI GGWCC 3 cut(s) 43, 1122, 1271
Sse9I AATT 8 cut(s) 261, 446, 891, 906, 944, 992, 1183, 1366
SsiI CCGC 2 cut(s) 218, 879
SspMI CTAG 2 cut(s) 731, 1077
StyD4I CCNGG 2 cut(s) 400, 1330
StyI CCWWGG 3 cut(s) 709, 974, 1076
TaaI ACNGT 4 cut(s) 389, 484, 872, 1425
TaiI ACGT 1 cut(s) 1284
TaqI TCGA 5 cut(s) 206, 237, 768, 1284, 1384
TasI AATT 8 cut(s) 261, 446, 891, 906, 944, 992, 1183, 1366
TfiI GAWTC 3 cut(s) 73, 393, 1159
Tru1I TTAA 3 cut(s) 264, 696, 1370
Tru9I TTAA 3 cut(s) 264, 696, 1370
TscAI CASTG 2 cut(s) 436, 500
TseI GCWGC 5 cut(s) 193, 270, 273, 422, 970
TspDTI ATGAA 4 cut(s) 709, 737, 1029, 1164
TspGWI ACGGA 2 cut(s) 714, 1284
TspRI CASTG 2 cut(s) 436, 500
Tth111I GACNNNGTC 1 cut(s) 1280
Van91I CCANNNNNTGG 1 cut(s) 980
VpaK11BI GGWCC 3 cut(s) 43, 1122, 1271
VspI ATTAAT 1 cut(s) 264
XapI RAATTY 2 cut(s) 992, 1366
XceI RCATGY 2 cut(s) 37, 817
XmaJI CCTAGG 1 cut(s) 1076
XmiI GTMKAC 2 cut(s) 736, 1384
XmnI GAANNNNTTC 1 cut(s) 1187
XspI CTAG 2 cut(s) 731, 1077
Zsp2I ATGCAT 2 cut(s) 649, 1228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.