FvH4_1g16103

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003099
Physical Location & Seq
Reverse (-)
9208803 .. 9213293
4491 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16103.t3

Sequence Viewer

Length: 927 bp
ATGGAGTTCTTGGGATCTTTCGACTTCTGGAATGATTATCAACAAAAAGCCACCACACAAGCCTTCATGCTTCGGGATAAGTCTTCTGACCATGACACTCTTATGGTGGCCTTCAGAGGAACCGAACCATTTGATGCAGATGCATGGTGCTCCGACTTTGATATCTCTTGGTATGAGCTTTCAGGGGTCGGAAAAATTCATGGCGGCTTTATGAAAGCTTTAGGGTTACGAAAGAATGTTGGTTGGCCTAAGGAAACCAAACAAGAAGATGGTCATCCAGACTTGGCTTACTATGCAATTAGGGATATGCTTAACAAGCTTTTGAGGCAAAATGATCAAGCTAGATACATATTGACAGGGCATAGTTTGGGAGGGGCATTAGCAATATTGTTTCCAGCAGTTTTGGCTCTTCACAATGAGCCATGGTTATTGGAGAGATTGCAAGGGGTATATACTTTTGGACAACCGAGGGTTGGAGATGAGAAGTTTGGTGAGTTCATGGAGGAAAAGTTGCAAGAACATAATGTTCGATATTATAGATTTGTTTATGGTAATGATATGGTTCCCAGGTTGCCTTATGATGACAAAGCCTTGATGTTCAAGCACTTTGGGACATGCCTCTACTACAATAGGCACTATAAAGTGAAGGTTGTGGAACATGAACCTAATAAAAACTACTTCTCACCACTAGGGGCAATACCAATGATAATAAACGCATTTTTCGAGCTAATTAGAAGCTTCACCATTGCAAAAGCAAAGGGGCCAGACTATAAGGAAGGTACATTTCTAAGAATTTTCAGAATAATTGGCCTGGTCTTTCCTGGGGTTCCAGCTCATAGCACTCAAGATTATGTGAATGCGACCAGGTTGGGGTCCTCGGACGTATTCCTCCTACCAAAGGACACTTCATCCCATGAGGATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

309

Amino Acids

35.48

Weight (kDa)

6.26

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 36 - 194 9.4e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 204
AclWI GGATC 1 cut(s) 22
AcsI RAATTY 2 cut(s) 195, 792
AcuI CTGAAG 1 cut(s) 97
AfaI GTAC 1 cut(s) 781
AfiI CCNNNNNNNGG 3 cut(s) 473, 870, 898
AgsI TTSAA 1 cut(s) 601
AjnI CCWGG 4 cut(s) 566, 810, 820, 863
AjuI GAANNNNNNNTTGG 2 cut(s) 889, 921
AluBI AGCT 7 cut(s) 178, 218, 319, 341, 727, 738, 833
AluI AGCT 7 cut(s) 178, 218, 319, 341, 727, 738, 833
Alw21I GWGCWC 1 cut(s) 152
AlwI GGATC 1 cut(s) 22
AoxI GGCC 4 cut(s) 108, 245, 761, 808
ApoI RAATTY 2 cut(s) 195, 792
AspS9I GGNCC 2 cut(s) 761, 873
AsuHPI GGTGA 3 cut(s) 503, 675, 733
AvaII GGWCC 1 cut(s) 873
AxyI CCTNAGG 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 152
BccI CCATC 1 cut(s) 263
BciT130I CCWGG 4 cut(s) 568, 812, 822, 865
BclI TGATCA 1 cut(s) 334
BfaI CTAG 2 cut(s) 342, 689
BisI GCNGC 1 cut(s) 205
BlsI GCNGC 1 cut(s) 206
Bme1390I CCNGG 4 cut(s) 568, 812, 822, 865
Bme18I GGWCC 1 cut(s) 873
BmgT120I GGNCC 2 cut(s) 761, 873
BmiI GGNNCC 5 cut(s) 121, 564, 762, 828, 874
BmrFI CCNGG 4 cut(s) 568, 812, 822, 865
BmsI GCATC 2 cut(s) 124, 130
BpiI GAAGAC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 828
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 5 cut(s) 422, 467, 566, 821, 876
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 3 cut(s) 473, 870, 898
Bse21I CCTNAGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 744
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 4 cut(s) 568, 812, 822, 865
BseDI CCNNGG 5 cut(s) 422, 467, 566, 821, 876
BseGI GGATG 3 cut(s) 274, 908, 925
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 3 cut(s) 473, 870, 898
BseMI GCAATG 1 cut(s) 744
BshFI GGCC 4 cut(s) 110, 247, 763, 810
BsiHKAI GWGCWC 1 cut(s) 152
BslFI GGGAC 1 cut(s) 625
BslI CCNNNNNNNGG 3 cut(s) 473, 870, 898
BsmFI GGGAC 1 cut(s) 625
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 4 cut(s) 110, 247, 763, 810
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 2 cut(s) 14, 334
Bsp19I CCATGG 1 cut(s) 422
BspACI CCGC 1 cut(s) 204
BspANI GGCC 4 cut(s) 110, 247, 763, 810
BspLI GGNNCC 5 cut(s) 121, 564, 762, 828, 874
BspPI GGATC 1 cut(s) 22
BspQI GCTCTTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 744
BssECI CCNNGG 5 cut(s) 422, 467, 566, 821, 876
BssMI GATC 2 cut(s) 14, 334
BssT1I CCWWGG 1 cut(s) 422
Bst2UI CCWGG 4 cut(s) 568, 812, 822, 865
Bst6I CTCTTC 1 cut(s) 414
BstDEI CTNAG 2 cut(s) 249, 788
BstDSI CCRYGG 1 cut(s) 422
BstENI CCTNNNNNAGG 1 cut(s) 896
BstF5I GGATG 3 cut(s) 274, 908, 925
BstKTI GATC 2 cut(s) 17, 337
BstMBI GATC 2 cut(s) 14, 334
BstMWI GCNNNNNNNGC 4 cut(s) 293, 316, 325, 404
BstNI CCWGG 4 cut(s) 568, 812, 822, 865
BstNSI RCATGY 1 cut(s) 618
BstSCI CCNGG 4 cut(s) 566, 810, 820, 863
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
Bsu36I CCTNAGG 1 cut(s) 249
BsuRI GGCC 4 cut(s) 110, 247, 763, 810
BtgI CCRYGG 1 cut(s) 422
BtsCI GGATG 3 cut(s) 274, 908, 925
Cfr13I GGNCC 2 cut(s) 761, 873
CsiI ACCWGGT 1 cut(s) 863
Csp6I GTAC 1 cut(s) 780
CviAII CATG 9 cut(s) 67, 92, 144, 200, 423, 499, 615, 659, 914
CviQI GTAC 1 cut(s) 780
DdeI CTNAG 2 cut(s) 249, 788
DpnI GATC 2 cut(s) 16, 336
DpnII GATC 2 cut(s) 14, 334
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco130I CCWWGG 1 cut(s) 422
Eco32I GATATC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 873
Eco57I CTGAAG 1 cut(s) 97
Eco81I CCTNAGG 1 cut(s) 249
EcoNI CCTNNNNNAGG 1 cut(s) 896
EcoO109I RGGNCCY 1 cut(s) 873
EcoRII CCWGG 4 cut(s) 566, 810, 820, 863
EcoRV GATATC 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 422
EcoT22I ATGCAT 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 422
FaeI CATG 9 cut(s) 70, 95, 147, 203, 426, 502, 618, 662, 917
FaqI GGGAC 1 cut(s) 625
FatI CATG 9 cut(s) 66, 91, 143, 199, 422, 498, 614, 658, 913
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 205
FokI GGATG 2 cut(s) 261, 895
Fsp4HI GCNGC 1 cut(s) 205
FspBI CTAG 2 cut(s) 342, 689
GluI GCNGC 1 cut(s) 205
HaeIII GGCC 4 cut(s) 110, 247, 763, 810
Hin1II CATG 9 cut(s) 70, 95, 147, 203, 426, 502, 618, 662, 917
HindIII AAGCTT 3 cut(s) 216, 317, 736
HphI GGTGA 3 cut(s) 503, 675, 733
Hpy188I TCNGA 6 cut(s) 88, 116, 154, 191, 800, 880
Hpy188III TCNNGA 4 cut(s) 28, 74, 278, 845
HpyAV CCTTC 4 cut(s) 73, 121, 640, 770
HpyCH4IV ACGT 1 cut(s) 882
HpyCH4V TGCA 6 cut(s) 137, 143, 296, 442, 514, 749
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 316, 325, 404
HpyF3I CTNAG 2 cut(s) 249, 788
HpySE526I ACGT 1 cut(s) 882
Hsp92II CATG 9 cut(s) 70, 95, 147, 203, 426, 502, 618, 662, 917
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 14, 334
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 155
LweI GCATC 2 cut(s) 124, 130
MabI ACCWGGT 1 cut(s) 863
MaeI CTAG 2 cut(s) 342, 689
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 1 cut(s) 225
MalI GATC 2 cut(s) 16, 336
MboI GATC 2 cut(s) 14, 334
MboII GAAGA 3 cut(s) 75, 278, 401
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 5 cut(s) 195, 297, 729, 792, 804
MmeI TCCRAC 3 cut(s) 169, 177, 454
MnlI CCTC 9 cut(s) 110, 318, 365, 462, 496, 629, 886, 899, 910
Mph1103I ATGCAT 1 cut(s) 145
MseI TTAA 1 cut(s) 312
MslI CAYNNNNRTG 2 cut(s) 101, 918
MspR9I CCNGG 4 cut(s) 568, 812, 822, 865
Mva1269I GAATGC 1 cut(s) 862
MvaI CCWGG 4 cut(s) 568, 812, 822, 865
MwoI GCNNNNNNNGC 4 cut(s) 293, 316, 325, 404
NcoI CCATGG 1 cut(s) 422
NdeII GATC 2 cut(s) 14, 334
NlaIII CATG 9 cut(s) 70, 95, 147, 203, 426, 502, 618, 662, 917
NlaIV GGNNCC 5 cut(s) 121, 564, 762, 828, 874
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 1 cut(s) 618
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 720
PctI GAATGC 1 cut(s) 862
PkrI GCNGC 1 cut(s) 206
PpuMI RGGWCCY 1 cut(s) 873
Psp5II RGGWCCY 1 cut(s) 873
Psp6I CCWGG 4 cut(s) 566, 810, 820, 863
PspGI CCWGG 4 cut(s) 566, 810, 820, 863
PspN4I GGNNCC 5 cut(s) 121, 564, 762, 828, 874
PspPI GGNCC 2 cut(s) 761, 873
PspPPI RGGWCCY 1 cut(s) 873
PsuI RGATCY 1 cut(s) 14
RsaI GTAC 1 cut(s) 781
RsaNI GTAC 1 cut(s) 780
RseI CAYNNNNRTG 2 cut(s) 101, 918
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 1 cut(s) 205
Sau3AI GATC 2 cut(s) 14, 334
Sau96I GGNCC 2 cut(s) 761, 873
ScrFI CCNGG 4 cut(s) 568, 812, 822, 865
SduI GDGCHC 1 cut(s) 152
SexAI ACCWGGT 1 cut(s) 863
SfaNI GCATC 2 cut(s) 124, 130
SinI GGWCC 1 cut(s) 873
SmiMI CAYNNNNRTG 2 cut(s) 101, 918
SmlI CTYRAG 1 cut(s) 843
SmoI CTYRAG 1 cut(s) 843
Sse9I AATT 5 cut(s) 195, 297, 729, 792, 804
SsiI CCGC 1 cut(s) 204
SspI AATATT 1 cut(s) 387
SspMI CTAG 2 cut(s) 342, 689
StyD4I CCNGG 4 cut(s) 566, 810, 820, 863
StyI CCWWGG 1 cut(s) 422
TaiI ACGT 1 cut(s) 885
TaqI TCGA 3 cut(s) 21, 529, 723
TasI AATT 5 cut(s) 195, 297, 729, 792, 804
TauI GCSGC 1 cut(s) 207
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TspDTI ATGAA 6 cut(s) 55, 188, 227, 487, 675, 897
VpaK11BI GGWCC 1 cut(s) 873
XagI CCTNNNNNAGG 1 cut(s) 896
XapI RAATTY 2 cut(s) 195, 792
XceI RCATGY 1 cut(s) 618
XspI CTAG 2 cut(s) 342, 689
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.