RLG00000017457

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003099
Physical Location & Seq
Reverse (-)
17046937 .. 17049707
2771 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017457

Sequence Viewer

Length: 1197 bp
ATGGCTTGTAACAAGAGTTTTTCCAGCAATTACATGCTACTGGACCCCAACGATGTGGGTTTCTTTGATCTGATTCACATCTTGATCTCCAATAACTTGGAGAAGAGAAAGTTCGTTGACTCCTCCGATGACAAAGAAGATGACTTTCAGCGCAGATGGCTCATATTTCTGTCTATTGTGGTGCAGAAGTTGCTGCAGTTTGTGTCGAAGCCAATGGCAGTTATTGGCCGGGCAATCGAAATGTTCTTGAACTTTTTGTCAATTAATGGCTGCAGCTTGCATGGACTGCTCCTAAATCTTTTGCGAGAGTATCAGGGAAAAGCTACAACACAAGCCTTTATGCTTCGGGACAGATCACGTGCTGACCATGATACCATTGTTGTTGCCTTTAGAGGAACCGAACCCTTTGATGCAGATGCATGGTGCTCTGACTTTGATATTTCGTGGTATGAGCTTGAAGGTGGAGTTAACGGAAAGATCCATGGTGGATTCATGAAAGCTCTAGGTTTACAGAAAAATATCGGTTGGCCAAAGAAAATCGAGCAAGATGACAAGCACCCCTTGGCTTACTATGCTGTTAGGGACACGCTGAAAACGCTACTAAGTGAAAATGATAAGGCGAGATATATCATAACAGGGCACAGTTTAGGCGGAGCGTTAGCAATTCTCTTCCCTGCAATTTTGGCATTACACGAGGAAAACGAGCTGTTGGAGAAATTGGAAGGAGTTTATACATTTGGCCAGCCACGGGTTGGAGACAAGGAATTTGGGCAATACATGGAAAATGAAGTATTGAAAGAGCACAATGTTAGGTATTTTAGGTTTGTTTATAGTAATGATATGGTGCCCAGGTTACCTTATGATGACAAGGCCCTCATGTTCAAGCACTTTGGGACCTGCCTCTACTATAACAGAAACTATGAAGCACAGATTCTTCCAGAAGAGCCAAACAAGAATTACTTCTCCCCATCGGTGCAAATAACTATGATGGTGAATGCATTTTATGAGCTGACGAGAAGCTTCACAATTCCAAATAAGTACGGACCTGACTACGTCGAAGGGTACTTCTTCAGATTGTTTAGGCTAAGTGGTTTGCTAATGCCAGGGGCATCAGCACATTGTCCCCAAGATTATGTGAATTTAACCAGATTGGGACCCTCAGATGTGTTCGTCTCACCTAAGCCCAAGCAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

399

Amino Acids

45.8

Weight (kDa)

6.09

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 127 - 288 4.9e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 905
AccB1I GGYRCC 1 cut(s) 844
AccB7I CCANNNNNTGG 1 cut(s) 752
AciI CCGC 1 cut(s) 651
AclWI GGATC 1 cut(s) 472
AcoI YGGCCR 3 cut(s) 226, 527, 739
AcsI RAATTY 2 cut(s) 764, 1138
AcuI CTGAAG 1 cut(s) 1054
AcvI CACGTG 1 cut(s) 359
AfaI GTAC 2 cut(s) 1040, 1064
AfiI CCNNNNNNNGG 2 cut(s) 748, 752
AgsI TTSAA 4 cut(s) 250, 458, 796, 883
AjnI CCWGG 2 cut(s) 848, 1102
AluBI AGCT 7 cut(s) 276, 323, 454, 500, 706, 1009, 1020
AluI AGCT 7 cut(s) 276, 323, 454, 500, 706, 1009, 1020
Alw21I GWGCWC 2 cut(s) 428, 804
Alw26I GTCTC 2 cut(s) 750, 1177
AlwI GGATC 1 cut(s) 472
AoxI GGCC 4 cut(s) 226, 527, 739, 870
ApeKI GCWGC 3 cut(s) 193, 270, 273
ApoI RAATTY 2 cut(s) 764, 1138
ArsI GACNNNNNNTTYG 2 cut(s) 749, 781
AseI ATTAAT 1 cut(s) 264
Asp700I GAANNNNTTC 1 cut(s) 959
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 5 cut(s) 43, 871, 894, 1043, 1154
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 1003, 1167
AvaII GGWCC 4 cut(s) 43, 894, 1043, 1154
BaeGI GKGCMC 2 cut(s) 642, 849
BaeI ACNNNNGTAYC 2 cut(s) 363, 396
BalI TGGCCA 2 cut(s) 529, 741
BanI GGYRCC 1 cut(s) 844
BauI CACGAG 1 cut(s) 692
BbrPI CACGTG 1 cut(s) 359
Bbv12I GWGCWC 2 cut(s) 428, 804
BbvI GCAGC 3 cut(s) 180, 257, 285
BccI CCATC 3 cut(s) 150, 976, 982
BciT130I CCWGG 2 cut(s) 850, 1104
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 2 cut(s) 750, 1177
BfaI CTAG 1 cut(s) 503
BfmI CTRYAG 2 cut(s) 194, 271
BfuAI ACCTGC 1 cut(s) 905
BisI GCNGC 3 cut(s) 194, 271, 274
BlsI GCNGC 3 cut(s) 195, 272, 275
Bme1390I CCNGG 3 cut(s) 230, 850, 1104
Bme18I GGWCC 4 cut(s) 43, 894, 1043, 1154
BmgT120I GGNCC 5 cut(s) 43, 871, 894, 1043, 1154
BmiI GGNNCC 6 cut(s) 45, 397, 846, 895, 1155, 1156
BmrFI CCNGG 3 cut(s) 230, 850, 1104
BmsI GCATC 3 cut(s) 400, 406, 1118
Bpu10I CCTNAGC 1 cut(s) 1179
BpuMI CCSGG 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 5 cut(s) 481, 561, 746, 848, 1103
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 752
Bse1I ACTGG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 77
BseBI CCWGG 2 cut(s) 850, 1104
BseDI CCNNGG 5 cut(s) 481, 561, 746, 848, 1103
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 748, 752
BseMII CTCAG 1 cut(s) 1173
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 2 cut(s) 642, 849
BseXI GCAGC 3 cut(s) 180, 257, 285
BsgI GTGCAG 1 cut(s) 203
BshFI GGCC 4 cut(s) 228, 529, 741, 872
BshNI GGYRCC 1 cut(s) 844
BsiHKAI GWGCWC 2 cut(s) 428, 804
BsiSI CCGG 1 cut(s) 229
BslFI GGGAC 5 cut(s) 362, 596, 907, 1107, 1167
BslI CCNNNNNNNGG 2 cut(s) 748, 752
BsmAI GTCTC 2 cut(s) 750, 1177
BsmBI CGTCTC 1 cut(s) 1177
BsmFI GGGAC 5 cut(s) 362, 596, 907, 1107, 1167
BsmI GAATGC 1 cut(s) 1000
BsnI GGCC 4 cut(s) 228, 529, 741, 872
Bsp1286I GDGCHC 4 cut(s) 428, 642, 804, 849
Bsp143I GATC 4 cut(s) 67, 84, 353, 477
Bsp19I CCATGG 1 cut(s) 481
BspACI CCGC 1 cut(s) 651
BspANI GGCC 4 cut(s) 228, 529, 741, 872
BspCNI CTCAG 1 cut(s) 1172
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 6 cut(s) 45, 397, 846, 895, 1155, 1156
BspMAI CTGCAG 2 cut(s) 198, 275
BspMI ACCTGC 1 cut(s) 905
BspPI GGATC 1 cut(s) 472
BspQI GCTCTTC 1 cut(s) 936
BspT107I GGYRCC 1 cut(s) 844
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 5 cut(s) 481, 561, 746, 848, 1103
BssMI GATC 4 cut(s) 67, 84, 353, 477
BssSI CACGAG 1 cut(s) 692
BssT1I CCWWGG 2 cut(s) 481, 561
Bst2BI CACGAG 1 cut(s) 692
Bst2UI CCWGG 2 cut(s) 850, 1104
Bst4CI ACNGT 2 cut(s) 644, 1194
Bst6I CTCTTC 3 cut(s) 98, 674, 936
BstAPI GCANNNNNTGC 2 cut(s) 190, 286
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 278, 743
BstDEI CTNAG 4 cut(s) 602, 1085, 1159, 1179
BstDSI CCRYGG 2 cut(s) 481, 746
BstEII GGTNACC 1 cut(s) 852
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 4 cut(s) 70, 87, 356, 480
BstMAI GTCTC 2 cut(s) 750, 1177
BstMBI GATC 4 cut(s) 67, 84, 353, 477
BstMWI GCNNNNNNNGC 6 cut(s) 157, 190, 286, 572, 595, 683
BstNI CCWGG 2 cut(s) 850, 1104
BstNSI RCATGY 1 cut(s) 37
BstPI GGTNACC 1 cut(s) 852
BstSCI CCNGG 3 cut(s) 228, 848, 1102
BstSFI CTRYAG 2 cut(s) 194, 271
BstSLI GKGCMC 2 cut(s) 642, 849
BstV1I GCAGC 3 cut(s) 180, 257, 285
BstX2I RGATCY 1 cut(s) 477
BstXI CCANNNNNNTGG 2 cut(s) 55, 97
BstYI RGATCY 1 cut(s) 477
BsuRI GGCC 4 cut(s) 228, 529, 741, 872
BtgI CCRYGG 2 cut(s) 481, 746
BveI ACCTGC 1 cut(s) 905
Cac8I GCNNGC 2 cut(s) 278, 743
CciI TCATGA 1 cut(s) 492
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 5 cut(s) 43, 871, 894, 1043, 1154
Csp6I GTAC 2 cut(s) 1039, 1063
CviAII CATG 8 cut(s) 34, 281, 368, 420, 482, 493, 778, 877
CviQI GTAC 2 cut(s) 1039, 1063
DdeI CTNAG 4 cut(s) 602, 1085, 1159, 1179
DpnI GATC 4 cut(s) 69, 86, 355, 479
DpnII GATC 4 cut(s) 67, 84, 353, 477
EaeI YGGCCR 3 cut(s) 226, 527, 739
Eam1104I CTCTTC 3 cut(s) 98, 674, 936
EarI CTCTTC 3 cut(s) 98, 674, 936
EciI GGCGGA 1 cut(s) 666
Eco130I CCWWGG 2 cut(s) 481, 561
Eco47I GGWCC 4 cut(s) 43, 894, 1043, 1154
Eco57I CTGAAG 1 cut(s) 1054
Eco72I CACGTG 1 cut(s) 359
Eco91I GGTNACC 1 cut(s) 852
EcoO109I RGGNCCY 3 cut(s) 871, 894, 1154
EcoO65I GGTNACC 1 cut(s) 852
EcoRII CCWGG 2 cut(s) 848, 1102
EcoT14I CCWWGG 2 cut(s) 481, 561
EcoT22I ATGCAT 2 cut(s) 421, 1000
ErhI CCWWGG 2 cut(s) 481, 561
Esp3I CGTCTC 1 cut(s) 1177
FaeI CATG 8 cut(s) 37, 284, 371, 423, 485, 496, 781, 880
FalI AAGNNNNNCTT 4 cut(s) 545, 577, 944, 976
FaqI GGGAC 5 cut(s) 362, 596, 907, 1107, 1167
FatI CATG 8 cut(s) 33, 280, 367, 419, 481, 492, 777, 876
Fnu4HI GCNGC 3 cut(s) 194, 271, 274
Fsp4HI GCNGC 3 cut(s) 194, 271, 274
FspBI CTAG 1 cut(s) 503
GlaI GCGC 1 cut(s) 152
GluI GCNGC 3 cut(s) 194, 271, 274
HaeIII GGCC 4 cut(s) 228, 529, 741, 872
HapII CCGG 1 cut(s) 229
HhaI GCGC 1 cut(s) 153
Hin1II CATG 8 cut(s) 37, 284, 371, 423, 485, 496, 781, 880
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HincII GTYRAC 2 cut(s) 118, 469
HindII GTYRAC 2 cut(s) 118, 469
HindIII AAGCTT 1 cut(s) 1018
HinfI GANTC 4 cut(s) 73, 119, 489, 931
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 1 cut(s) 229
HphI GGTGA 2 cut(s) 1003, 1167
Hpy166II GTNNAC 3 cut(s) 118, 469, 509
Hpy188I TCNGA 5 cut(s) 72, 127, 430, 1073, 1162
Hpy188III TCNNGA 5 cut(s) 82, 247, 347, 493, 938
Hpy8I GTNNAC 3 cut(s) 118, 469, 509
Hpy99I CGWCG 1 cut(s) 1058
HpyAV CCTTC 3 cut(s) 452, 716, 1052
HpyCH4III ACNGT 2 cut(s) 644, 1194
HpyCH4IV ACGT 2 cut(s) 358, 1053
HpyCH4V TGCA 9 cut(s) 184, 196, 273, 280, 413, 419, 677, 976, 998
HpyF10VI GCNNNNNNNGC 6 cut(s) 157, 190, 286, 572, 595, 683
HpyF3I CTNAG 4 cut(s) 602, 1085, 1159, 1179
HpySE526I ACGT 2 cut(s) 358, 1053
Hsp92II CATG 8 cut(s) 37, 284, 371, 423, 485, 496, 781, 880
HspAI GCGC 1 cut(s) 151
KflI GGGWCCC 1 cut(s) 1154
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 4 cut(s) 67, 84, 353, 477
LguI GCTCTTC 1 cut(s) 936
LmnI GCTCC 2 cut(s) 294, 653
Lsp1109I GCAGC 3 cut(s) 180, 257, 285
LweI GCATC 3 cut(s) 400, 406, 1118
MaeI CTAG 1 cut(s) 503
MaeII ACGT 2 cut(s) 358, 1053
MaeIII GTNAC 2 cut(s) 8, 852
MalI GATC 4 cut(s) 69, 86, 355, 479
MboI GATC 4 cut(s) 67, 84, 353, 477
MboII GAAGA 6 cut(s) 115, 149, 661, 926, 953, 1060
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 4 cut(s) 428, 642, 804, 849
MlsI TGGCCA 2 cut(s) 529, 741
MluCI AATT 9 cut(s) 28, 261, 663, 678, 716, 764, 955, 1026, 1138
MluNI TGGCCA 2 cut(s) 529, 741
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 690, 733
MnlI CCTC 6 cut(s) 133, 386, 688, 884, 911, 1168
Mox20I TGGCCA 2 cut(s) 529, 741
Mph1103I ATGCAT 2 cut(s) 421, 1000
MroXI GAANNNNTTC 1 cut(s) 959
MscI TGGCCA 2 cut(s) 529, 741
MseI TTAA 3 cut(s) 264, 468, 1142
Msp20I TGGCCA 2 cut(s) 529, 741
MspI CCGG 1 cut(s) 229
MspR9I CCNGG 3 cut(s) 230, 850, 1104
Mva1269I GAATGC 1 cut(s) 1000
MvaI CCWGG 2 cut(s) 850, 1104
MwoI GCNNNNNNNGC 6 cut(s) 157, 190, 286, 572, 595, 683
NciI CCSGG 1 cut(s) 230
NcoI CCATGG 1 cut(s) 481
NdeII GATC 4 cut(s) 67, 84, 353, 477
NlaIII CATG 8 cut(s) 37, 284, 371, 423, 485, 496, 781, 880
NlaIV GGNNCC 6 cut(s) 45, 397, 846, 895, 1155, 1156
NsiI ATGCAT 2 cut(s) 421, 1000
NspI RCATGY 1 cut(s) 37
PagI TCATGA 1 cut(s) 492
PciSI GCTCTTC 1 cut(s) 936
PcsI WCGNNNNNNNCGW 1 cut(s) 699
PctI GAATGC 1 cut(s) 1000
PdmI GAANNNNTTC 1 cut(s) 959
PfeI GAWTC 3 cut(s) 73, 489, 931
PflFI GACNNNGTC 1 cut(s) 1052
PflMI CCANNNNNTGG 1 cut(s) 752
PkrI GCNGC 3 cut(s) 195, 272, 275
PleI GAGTC 1 cut(s) 113
PmaCI CACGTG 1 cut(s) 359
PmlI CACGTG 1 cut(s) 359
PpsI GAGTC 1 cut(s) 113
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 2 cut(s) 894, 1154
PshBI ATTAAT 1 cut(s) 264
Psp5II RGGWCCY 2 cut(s) 894, 1154
Psp6I CCWGG 2 cut(s) 848, 1102
PspCI CACGTG 1 cut(s) 359
PspEI GGTNACC 1 cut(s) 852
PspGI CCWGG 2 cut(s) 848, 1102
PspN4I GGNNCC 6 cut(s) 45, 397, 846, 895, 1155, 1156
PspPI GGNCC 5 cut(s) 43, 871, 894, 1043, 1154
PspPPI RGGWCCY 2 cut(s) 894, 1154
PstI CTGCAG 2 cut(s) 198, 275
PsuI RGATCY 1 cut(s) 477
PsyI GACNNNGTC 1 cut(s) 1052
RsaI GTAC 2 cut(s) 1040, 1064
RsaNI GTAC 2 cut(s) 1039, 1063
SapI GCTCTTC 1 cut(s) 936
SaqAI TTAA 3 cut(s) 264, 468, 1142
SatI GCNGC 3 cut(s) 194, 271, 274
Sau3AI GATC 4 cut(s) 67, 84, 353, 477
Sau96I GGNCC 5 cut(s) 43, 871, 894, 1043, 1154
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 3 cut(s) 230, 850, 1104
SduI GDGCHC 4 cut(s) 428, 642, 804, 849
SfaNI GCATC 3 cut(s) 400, 406, 1118
SfcI CTRYAG 2 cut(s) 194, 271
SinI GGWCC 4 cut(s) 43, 894, 1043, 1154
Sse9I AATT 9 cut(s) 28, 261, 663, 678, 716, 764, 955, 1026, 1138
SsiI CCGC 1 cut(s) 651
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 3 cut(s) 228, 848, 1102
StyI CCWWGG 2 cut(s) 481, 561
TaaI ACNGT 2 cut(s) 644, 1194
TaiI ACGT 2 cut(s) 361, 1056
TaqI TCGA 4 cut(s) 206, 237, 540, 1056
TasI AATT 9 cut(s) 28, 261, 663, 678, 716, 764, 955, 1026, 1138
TfiI GAWTC 3 cut(s) 73, 489, 931
Tru1I TTAA 3 cut(s) 264, 468, 1142
Tru9I TTAA 3 cut(s) 264, 468, 1142
TseI GCWGC 3 cut(s) 193, 270, 273
TspDTI ATGAA 4 cut(s) 481, 509, 801, 936
TspGWI ACGGA 2 cut(s) 486, 1056
Tth111I GACNNNGTC 1 cut(s) 1052
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 4 cut(s) 43, 894, 1043, 1154
VspI ATTAAT 1 cut(s) 264
XapI RAATTY 2 cut(s) 764, 1138
XceI RCATGY 1 cut(s) 37
XcmI CCANNNNNNNNNTGG 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 959
XspI CTAG 1 cut(s) 503
Zsp2I ATGCAT 2 cut(s) 421, 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.