MD15G1284700.v1.1

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003099
Physical Location & Seq
Reverse (-)
25858508 .. 25862475
3968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1284700.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGGCAGCTGCTTGTGATAAGTGTTTCTCATGCAATTACATGCTCCTTGAACCAAAGGATGTGGATTTGTTTGATCTGTTTCACATCTTGTTTTCCAATAACTTAGAAAGGAGGAGGTTCGTTGACTCTTCTGAGGAAACTGAGGACGACTTTCAGCGCAGATGGCTCATATTTCTCTCTATTGTTGTCCAAAAATTGCTTCAATTTATGGCCAAGCCAGTGGCAGCTATTGGCTGGGGGATCGAGATGTGCTTGAATCTTGTCTCAAGTAATCAAAACTTGGTCGGACTCCTTCTAAATTTACTGCGAGGTAACGTGATAATACCAGATAAATCCTCAAGAACGTTCTTGTCGCTTATTGGAAATTTGGACAAGCGAGTAGAGCTGGACGAAAGCATCAAACCTGGAGATGGCAAGTACAACGCAGCACTTAGTATGATGGCGTCTAAAATATCATATGAGAACACATTCTACATCCAAACTGTTGTCCAGGATCATTGGAAGATGGAGTTCTTGGGCTCTTACGACTTTTGGAATGATTATCAAGAAAAAGCCACAACACAAGCCTGTATGTTCCGTGACAGAAGTGGTGACCATGACACAATTGTTGTGGCCTTCAGAGGCACTGAACCCTTTGATGCGGATGCATGGTGCTCCGACTTTGACATCTCCTGGTATCAACTTGAAGGGGTTGGAAAGATCCATAGTGGCTTCATGAAAGCTCTAGGCTTACAAAAGAATGTCGGTTGGCCGGAGCCTGATCAAATCAAAGAAGACGGTAGCCGCCCAGCCTTGGCTTACTATGCCATTAGAGATATGCTGAAAAAACTTCTTCTGAAAAATGAAAAAGCAAAGTTTATAATAACTGGGCACAGTTTAGGTGGGGCACTAGCAATTCTCTTTCCGGCAGTTTTAACATTTCACAAAGGGAAGGATTCAGATTTGCTGTTGGAGAGGTTGGAGGGAGTCTATACATTTGGGCAGCCTAGGGTTGGAGATAAGGCTTTTGGGGAGTACATGGAAAAGAAAATGATAGAGTATAAGATTAGGTATTTTAGGTTTGTTTATGGGAATGACATGGTGCCCAGGTTGCCTTATGATGACAAAGCTCTAATGTTCAAGCACTTTGGGACTTGCCTCTACTATAATAGGAATTATGAAGTTCAGGTTGTTCCAGAAGAGCCAAACAAGAATTACTTCTCTCCAACAGGGACAATACCAATGATAATGAATGCTTTTGGTGAGCTCGCAAGAAGTTTCACCATTCCAAAAAAGTTGGGACCAAACTACAATGAAGGTCCCTTGTTCAAGTTGTTTAGGATAATTGGTTTGCTACTGCCAGGAGCACCAGCACATTGTCCCCAAGATTATGTAAATGCCACCAGATTAGGATCCTCGCATGTGTTCCTCCCTCGCACGAATTCAATGAAACCAGAATCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

482

Amino Acids

55.0

Weight (kDa)

6.53

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 203 - 367 2e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 860
AccB1I GGYRCC 1 cut(s) 1081
AciI CCGC 2 cut(s) 641, 784
AclI AACGTT 1 cut(s) 344
AclWI GGATC 5 cut(s) 248, 501, 694, 1386, 1399
AcoI YGGCCR 2 cut(s) 210, 749
AcsI RAATTY 3 cut(s) 298, 364, 1420
AcuI CTGAAG 1 cut(s) 601
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 2 cut(s) 419, 1016
AfiI CCNNNNNNNGG 3 cut(s) 410, 793, 992
AgsI TTSAA 7 cut(s) 50, 203, 256, 686, 1120, 1309, 1425
AjnI CCWGG 5 cut(s) 403, 489, 671, 1085, 1339
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
AluBI AGCT 6 cut(s) 8, 227, 385, 722, 1109, 1246
AluI AGCT 6 cut(s) 8, 227, 385, 722, 1109, 1246
Alw21I GWGCWC 3 cut(s) 656, 1248, 1348
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 5 cut(s) 248, 501, 694, 1386, 1399
AoxI GGCC 3 cut(s) 210, 612, 749
ApeKI GCWGC 5 cut(s) 5, 8, 224, 425, 982
ApoI RAATTY 3 cut(s) 298, 364, 1420
Asp700I GAANNNNTTC 2 cut(s) 467, 1196
AspA2I CCTAGG 1 cut(s) 986
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 1280, 1298
AsuHPI GGTGA 3 cut(s) 602, 1252, 1253
AvaII GGWCC 2 cut(s) 1280, 1298
AvrII CCTAGG 1 cut(s) 986
BaeGI GKGCMC 3 cut(s) 873, 889, 1086
BalI TGGCCA 1 cut(s) 212
BamHI GGATCC 1 cut(s) 1391
BanI GGYRCC 1 cut(s) 1081
BanII GRGCYC 2 cut(s) 521, 1248
BbsI GAAGAC 1 cut(s) 780
Bbv12I GWGCWC 3 cut(s) 656, 1248, 1348
BbvI GCAGC 4 cut(s) 17, 236, 437, 994
BccI CCATC 4 cut(s) 156, 404, 433, 499
BciT130I CCWGG 5 cut(s) 405, 491, 673, 1087, 1341
BclI TGATCA 1 cut(s) 760
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 3 cut(s) 725, 890, 987
BisI GCNGC 6 cut(s) 6, 9, 225, 426, 784, 983
BlnI CCTAGG 1 cut(s) 986
BlsI GCNGC 6 cut(s) 7, 10, 226, 427, 785, 984
Bme1390I CCNGG 5 cut(s) 405, 491, 673, 1087, 1341
Bme18I GGWCC 2 cut(s) 1280, 1298
BmgT120I GGNCC 2 cut(s) 1280, 1298
BmiI GGNNCC 5 cut(s) 756, 1083, 1281, 1300, 1393
BmrFI CCNGG 5 cut(s) 405, 491, 673, 1087, 1341
BmrI ACTGGG 1 cut(s) 876
BmsI GCATC 3 cut(s) 405, 628, 634
BmuI ACTGGG 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 780
BpmI CTGGAG 1 cut(s) 426
BpuEI CTTGAG 2 cut(s) 250, 322
BsaBI GATNNNNATC 1 cut(s) 1390
BsaHI GRCGYC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 792, 986, 1085
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 793, 992
Bse1I ACTGG 2 cut(s) 218, 871
Bse8I GATNNNNATC 1 cut(s) 1390
BseBI CCWGG 5 cut(s) 405, 491, 673, 1087, 1341
BseDI CCNNGG 3 cut(s) 792, 986, 1085
BseGI GGATG 3 cut(s) 64, 474, 649
BseJI GATNNNNATC 1 cut(s) 1390
BseLI CCNNNNNNNGG 3 cut(s) 410, 793, 992
BseMII CTCAG 2 cut(s) 123, 132
BseNI ACTGG 2 cut(s) 218, 871
BseRI GAGGAG 1 cut(s) 127
BseSI GKGCMC 3 cut(s) 873, 889, 1086
BseXI GCAGC 4 cut(s) 17, 236, 437, 994
BseYI CCCAGC 2 cut(s) 234, 787
BshFI GGCC 3 cut(s) 212, 614, 751
BshNI GGYRCC 1 cut(s) 1081
BsiHKAI GWGCWC 3 cut(s) 656, 1248, 1348
BsiSI CCGG 2 cut(s) 752, 905
BslFI GGGAC 5 cut(s) 1144, 1225, 1284, 1293, 1344
BslI CCNNNNNNNGG 3 cut(s) 410, 793, 992
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 5 cut(s) 1144, 1225, 1284, 1293, 1344
BsmI GAATGC 1 cut(s) 1237
BsnI GGCC 3 cut(s) 212, 614, 751
Bsp1286I GDGCHC 7 cut(s) 521, 656, 873, 889, 1086, 1248, 1348
Bsp143I GATC 6 cut(s) 73, 240, 493, 699, 760, 1391
BspACI CCGC 2 cut(s) 641, 784
BspANI GGCC 3 cut(s) 212, 614, 751
BspCNI CTCAG 2 cut(s) 124, 133
BspHI TCATGA 1 cut(s) 714
BspLI GGNNCC 5 cut(s) 756, 1083, 1281, 1300, 1393
BspPI GGATC 5 cut(s) 248, 501, 694, 1386, 1399
BspQI GCTCTTC 1 cut(s) 1173
BspT107I GGYRCC 1 cut(s) 1081
BsrI ACTGG 2 cut(s) 218, 871
BssECI CCNNGG 3 cut(s) 792, 986, 1085
BssMI GATC 6 cut(s) 73, 240, 493, 699, 760, 1391
BssNI GRCGYC 1 cut(s) 443
BssT1I CCWWGG 2 cut(s) 792, 986
Bst2UI CCWGG 5 cut(s) 405, 491, 673, 1087, 1341
Bst4CI ACNGT 3 cut(s) 484, 779, 875
Bst6I CTCTTC 2 cut(s) 133, 1173
BstACI GRCGYC 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 1248
BstDEI CTNAG 4 cut(s) 103, 132, 141, 431
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 3 cut(s) 64, 474, 649
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 6 cut(s) 76, 243, 496, 702, 763, 1394
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 6 cut(s) 73, 240, 493, 699, 760, 1391
BstMWI GCNNNNNNNGC 4 cut(s) 163, 382, 803, 1090
BstNI CCWGG 5 cut(s) 405, 491, 673, 1087, 1341
BstNSI RCATGY 2 cut(s) 43, 1403
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 5 cut(s) 403, 489, 671, 1085, 1339
BstSLI GKGCMC 3 cut(s) 873, 889, 1086
BstV1I GCAGC 4 cut(s) 17, 236, 437, 994
BstV2I GAAGAC 1 cut(s) 780
BstX2I RGATCY 2 cut(s) 699, 1391
BstXI CCANNNNNNTGG 1 cut(s) 220
BstYI RGATCY 2 cut(s) 699, 1391
BsuRI GGCC 3 cut(s) 212, 614, 751
BtsCI GGATG 3 cut(s) 64, 474, 649
BtsIMutI CAGTG 2 cut(s) 225, 624
Cac8I GCNNGC 1 cut(s) 1248
CciI TCATGA 1 cut(s) 714
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 2 cut(s) 1280, 1298
CseI GACGC 1 cut(s) 432
Csp6I GTAC 2 cut(s) 418, 1015
CspCI CAANNNNNGTGG 4 cut(s) 42, 77, 591, 626
CviAII CATG 8 cut(s) 30, 40, 596, 648, 715, 1018, 1078, 1400
CviQI GTAC 2 cut(s) 418, 1015
DdeI CTNAG 4 cut(s) 103, 132, 141, 431
DpnI GATC 6 cut(s) 75, 242, 495, 701, 762, 1393
DpnII GATC 6 cut(s) 73, 240, 493, 699, 760, 1391
EaeI YGGCCR 2 cut(s) 210, 749
Eam1104I CTCTTC 2 cut(s) 133, 1173
EarI CTCTTC 2 cut(s) 133, 1173
Ecl136II GAGCTC 1 cut(s) 1246
Eco130I CCWWGG 2 cut(s) 792, 986
Eco24I GRGCYC 2 cut(s) 521, 1248
Eco47I GGWCC 2 cut(s) 1280, 1298
Eco53kI GAGCTC 1 cut(s) 1246
Eco57I CTGAAG 1 cut(s) 601
Eco91I GGTNACC 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 1246
EcoO109I RGGNCCY 1 cut(s) 1298
EcoO65I GGTNACC 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 1420
EcoRII CCWGG 5 cut(s) 403, 489, 671, 1085, 1339
EcoT14I CCWWGG 2 cut(s) 792, 986
EcoT22I ATGCAT 1 cut(s) 649
EcoT38I GRGCYC 2 cut(s) 521, 1248
ErhI CCWWGG 2 cut(s) 792, 986
FaeI CATG 8 cut(s) 33, 43, 599, 651, 718, 1021, 1081, 1403
FalI AAGNNNNNCTT 2 cut(s) 1181, 1213
FaqI GGGAC 5 cut(s) 1144, 1225, 1284, 1293, 1344
FatI CATG 8 cut(s) 29, 39, 595, 647, 714, 1017, 1077, 1399
FauNDI CATATG 1 cut(s) 457
FbaI TGATCA 1 cut(s) 760
Fnu4HI GCNGC 6 cut(s) 6, 9, 225, 426, 784, 983
FokI GGATG 3 cut(s) 71, 461, 656
FriOI GRGCYC 2 cut(s) 521, 1248
Fsp4HI GCNGC 6 cut(s) 6, 9, 225, 426, 784, 983
FspBI CTAG 3 cut(s) 725, 890, 987
GlaI GCGC 1 cut(s) 158
GluI GCNGC 6 cut(s) 6, 9, 225, 426, 784, 983
GsaI CCCAGC 2 cut(s) 238, 791
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 3 cut(s) 212, 614, 751
HapII CCGG 2 cut(s) 752, 905
HgaI GACGC 1 cut(s) 432
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 8 cut(s) 33, 43, 599, 651, 718, 1021, 1081, 1403
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 6 cut(s) 125, 256, 288, 935, 966, 1436
HpaII CCGG 2 cut(s) 752, 905
HphI GGTGA 3 cut(s) 602, 1252, 1253
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 6 cut(s) 133, 287, 620, 658, 837, 940
Hpy188III TCNNGA 5 cut(s) 244, 339, 545, 715, 1175
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 5 cut(s) 302, 625, 680, 925, 1289
HpyCH4III ACNGT 3 cut(s) 484, 779, 875
HpyCH4IV ACGT 2 cut(s) 315, 344
HpyCH4V TGCA 2 cut(s) 33, 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 163, 382, 803, 1090
HpyF3I CTNAG 4 cut(s) 103, 132, 141, 431
HpySE526I ACGT 2 cut(s) 315, 344
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 8 cut(s) 33, 43, 599, 651, 718, 1021, 1081, 1403
HspAI GCGC 1 cut(s) 157
Ksp22I TGATCA 1 cut(s) 760
Kzo9I GATC 6 cut(s) 73, 240, 493, 699, 760, 1391
LguI GCTCTTC 1 cut(s) 1173
LmnI GCTCC 4 cut(s) 48, 659, 754, 1343
Lsp1109I GCAGC 4 cut(s) 17, 236, 437, 994
LweI GCATC 3 cut(s) 405, 628, 634
MaeI CTAG 3 cut(s) 725, 890, 987
MaeII ACGT 2 cut(s) 315, 344
MaeIII GTNAC 3 cut(s) 311, 578, 590
MalI GATC 6 cut(s) 75, 242, 495, 701, 762, 1393
MboI GATC 6 cut(s) 73, 240, 493, 699, 760, 1391
MboII GAAGA 5 cut(s) 120, 514, 785, 824, 1190
MfeI CAATTG 1 cut(s) 603
MflI RGATCY 2 cut(s) 699, 1391
MhlI GDGCHC 7 cut(s) 521, 656, 873, 889, 1086, 1248, 1348
MlsI TGGCCA 1 cut(s) 212
MluNI TGGCCA 1 cut(s) 212
MlyI GAGTC 3 cut(s) 119, 282, 975
MmeI TCCRAC 7 cut(s) 265, 673, 681, 930, 939, 973, 1229
Mox20I TGGCCA 1 cut(s) 212
Mph1103I ATGCAT 1 cut(s) 649
MroXI GAANNNNTTC 2 cut(s) 467, 1196
MscI TGGCCA 1 cut(s) 212
MseI TTAA 1 cut(s) 914
Msp20I TGGCCA 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 752, 905
MspR9I CCNGG 5 cut(s) 405, 491, 673, 1087, 1341
MunI CAATTG 1 cut(s) 603
Mva1269I GAATGC 1 cut(s) 1237
MvaI CCWGG 5 cut(s) 405, 491, 673, 1087, 1341
MwoI GCNNNNNNNGC 4 cut(s) 163, 382, 803, 1090
NdeI CATATG 1 cut(s) 457
NdeII GATC 6 cut(s) 73, 240, 493, 699, 760, 1391
NlaIII CATG 8 cut(s) 33, 43, 599, 651, 718, 1021, 1081, 1403
NlaIV GGNNCC 5 cut(s) 756, 1083, 1281, 1300, 1393
NmuCI GTSAC 2 cut(s) 578, 590
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 2 cut(s) 43, 1403
PagI TCATGA 1 cut(s) 714
PciSI GCTCTTC 1 cut(s) 1173
PctI GAATGC 1 cut(s) 1237
PdmI GAANNNNTTC 2 cut(s) 467, 1196
PfeI GAWTC 3 cut(s) 256, 935, 1436
PfoI TCCNGGA 1 cut(s) 489
PkrI GCNGC 6 cut(s) 7, 10, 226, 427, 785, 984
PleI GAGTC 3 cut(s) 119, 282, 974
PpsI GAGTC 3 cut(s) 119, 282, 974
PpuMI RGGWCCY 1 cut(s) 1298
PsiI TTATAA 1 cut(s) 860
Psp124BI GAGCTC 1 cut(s) 1248
Psp1406I AACGTT 1 cut(s) 344
Psp5II RGGWCCY 1 cut(s) 1298
Psp6I CCWGG 5 cut(s) 403, 489, 671, 1085, 1339
PspEI GGTNACC 1 cut(s) 590
PspFI CCCAGC 2 cut(s) 234, 787
PspGI CCWGG 5 cut(s) 403, 489, 671, 1085, 1339
PspN4I GGNNCC 5 cut(s) 756, 1083, 1281, 1300, 1393
PspPI GGNCC 2 cut(s) 1280, 1298
PspPPI RGGWCCY 1 cut(s) 1298
PsrI GAACNNNNNNTAC 2 cut(s) 455, 487
PsuI RGATCY 2 cut(s) 699, 1391
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 419, 1016
RsaNI GTAC 2 cut(s) 418, 1015
SacI GAGCTC 1 cut(s) 1248
SapI GCTCTTC 1 cut(s) 1173
SaqAI TTAA 1 cut(s) 914
SatI GCNGC 6 cut(s) 6, 9, 225, 426, 784, 983
Sau3AI GATC 6 cut(s) 73, 240, 493, 699, 760, 1391
Sau96I GGNCC 2 cut(s) 1280, 1298
SchI GAGTC 3 cut(s) 119, 282, 975
ScrFI CCNGG 5 cut(s) 405, 491, 673, 1087, 1341
SduI GDGCHC 7 cut(s) 521, 656, 873, 889, 1086, 1248, 1348
SfaNI GCATC 3 cut(s) 405, 628, 634
SinI GGWCC 2 cut(s) 1280, 1298
SmlI CTYRAG 2 cut(s) 265, 337
SmoI CTYRAG 2 cut(s) 265, 337
SsiI CCGC 2 cut(s) 641, 784
SspMI CTAG 3 cut(s) 725, 890, 987
SstI GAGCTC 1 cut(s) 1248
StyD4I CCNGG 5 cut(s) 403, 489, 671, 1085, 1339
StyI CCWWGG 2 cut(s) 792, 986
TaaI ACNGT 3 cut(s) 484, 779, 875
TaiI ACGT 2 cut(s) 318, 347
TaqI TCGA 1 cut(s) 243
TatI WGTACW 2 cut(s) 417, 1014
TauI GCSGC 1 cut(s) 786
TfiI GAWTC 3 cut(s) 256, 935, 1436
Tru1I TTAA 1 cut(s) 914
Tru9I TTAA 1 cut(s) 914
TscAI CASTG 2 cut(s) 225, 631
TseFI GTSAC 2 cut(s) 578, 590
TseI GCWGC 5 cut(s) 5, 8, 224, 425, 982
Tsp45I GTSAC 2 cut(s) 578, 590
TspDTI ATGAA 7 cut(s) 703, 731, 858, 1173, 1244, 1308, 1442
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 2 cut(s) 225, 631
VpaK11BI GGWCC 2 cut(s) 1280, 1298
XapI RAATTY 3 cut(s) 298, 364, 1420
XceI RCATGY 2 cut(s) 43, 1403
XmaJI CCTAGG 1 cut(s) 986
XmnI GAANNNNTTC 2 cut(s) 467, 1196
XspI CTAG 3 cut(s) 725, 890, 987
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.