FvH4_1g16104

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003099
Physical Location & Seq
Reverse (-)
9213886 .. 9217149
3264 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16104.t1

Sequence Viewer

Length: 1428 bp
ATGGCTGCTTGTAACAAGAATTTCTCCAGCAGTTATATGCTACTGGAACCCCATGATGTGGGATTCTTTGATCTGATTCACATCTTGTTCTCCAATAACTTGGAGCACAGAAAGTTCGTAGACTCCTCCGATGACAAAGAATATGACTTTCAGCGCAGATGGCTCATATTTCTGTCAATTGTGGTGCAAAAGCTGCTGCAGTTTGTATCGAAGCCAATGGCAGTTATCGGCTGGGCAATCGAAATGTTCTTGAACTTTTTGTCACTTAATGTATGCAGCTTGCGTGGACTGCTCCTAAATCTTTCGCAAGGCAAGATTAGCATACCAGATAACACCTCAGGAACATACTTGTCGTTTATTGGACATCTGGATAAGCGAGTAAGGTTAGACAGTAGAATCAAACCTGGAGATTGCAGATATAATGCAGCACTCAGTATGATGGCTTCTAAACTTTCATATGAGAACCACGCCTGCATCAAAACTACAGTCGAAGATCACTGGAAGATGGAGTTCGTGGGCTTTTACGATTTCTGGAACGAGTATCAAGGAAAAGCTACAACTCAAGCCTTTATGCTCCGGGACAGATCTAGTGATGACCATGACACCATTGTTGTTGCCTTTAGAGGAACTGAACCCTTTGATGCAGATGCATGGTGCTCCGACTTTGATATTTCGTGGTACGAGCTTGAAGGAGGAGTTAATGGAAGGATTCATGGGGGCTTCATGAAAGCTCTGGGTTTACAGAAGAATGTCGGTTGGCCAAAGAATATAGATCAAGATGATAAGCACCCATTAGCTTACTATGCTATTAGGGACATGCTGAAAAAGCTTCTGAGTGAAAATGATAAGGCGAGATACGTCATAACAGGTCACAGTTTAGGTGGAGCGTTAGCGATTCTCTTCCCTGCAATTCTGGCATTGCACGAGGAAAACGAGCTGTTGGAGAAAATGGAAGGAGTTTATACATTTGGGCAGCCTAGGGTTGGTGACAAGGAATTTGGGCAGTACATGGAAAATGAAGTATTGACAGAGCACAATGTTAGGTACTTTAGGTTTGTTTATAGTAATGATATGGTGCCCAGGTTGCCCTATGATGACAAGGCCCTCATGTTCAAGCACTTCGGGACCTGCCTCTACTATAACAGAAACTATGAAGCACAGATTCTTCCAGAAGAGCCAAACAAGAATTACTTCTCCCTGTGGGTGCAAATGCCTATGATGGTGAATGCATTTTATGAGCTGACAAGAAGCTTCACACTTCCACGAAAGCATGGACCTGACTACGTCGAAGGGTACTTCTTCAGATTGTTTAGGCTAACTGGTTTGCTAATGCCAGGGGCACCAGCACATTGTCCCCAAGACTATGTGAACTTAACCAGATTGGGACCTCCAGATGTGTTAGTCTCCCCTAAGCCCAAGCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

476

Amino Acids

54.97

Weight (kDa)

6.12

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 204 - 365 1.8e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1136
AccB1I GGYRCC 2 cut(s) 1075, 1339
AccB7I CCANNNNNTGG 1 cut(s) 58
AccI GTMKAC 1 cut(s) 120
AcoI YGGCCR 1 cut(s) 758
AcsI RAATTY 2 cut(s) 19, 995
AcuI CTGAAG 1 cut(s) 1285
AfaI GTAC 4 cut(s) 680, 1007, 1046, 1295
AfiI CCNNNNNNNGG 2 cut(s) 58, 983
AgsI TTSAA 3 cut(s) 253, 689, 1114
AjnI CCWGG 3 cut(s) 403, 1079, 1333
Alw21I GWGCWC 3 cut(s) 108, 659, 1035
Alw26I GTCTC 1 cut(s) 1408
AoxI GGCC 2 cut(s) 758, 1101
ApeKI GCWGC 6 cut(s) 5, 193, 196, 276, 425, 973
ApoI RAATTY 2 cut(s) 19, 995
ArsI GACNNNNNNTTYG 4 cut(s) 471, 503, 980, 1012
Asp700I GAANNNNTTC 1 cut(s) 1190
AspA2I CCTAGG 1 cut(s) 977
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 4 cut(s) 1102, 1125, 1274, 1385
AsuC2I CCSGG 1 cut(s) 578
AsuHPI GGTGA 2 cut(s) 998, 1234
AvaII GGWCC 3 cut(s) 1125, 1274, 1385
AvrII CCTAGG 1 cut(s) 977
AxyI CCTNAGG 1 cut(s) 337
BaeGI GKGCMC 2 cut(s) 1080, 1342
BalI TGGCCA 1 cut(s) 760
BanI GGYRCC 2 cut(s) 1075, 1339
BarI GAAGNNNNNNTAC 2 cut(s) 427, 459
BauI CACGAG 1 cut(s) 923
Bbv12I GWGCWC 3 cut(s) 108, 659, 1035
BbvI GCAGC 5 cut(s) 180, 183, 288, 437, 985
BccI CCATC 4 cut(s) 153, 433, 499, 1213
BciT130I CCWGG 3 cut(s) 405, 1081, 1335
BcnI CCSGG 1 cut(s) 578
BcoDI GTCTC 1 cut(s) 1408
BfaI CTAG 2 cut(s) 588, 978
BfmI CTRYAG 2 cut(s) 197, 483
BfuAI ACCTGC 1 cut(s) 1136
BglII AGATCT 1 cut(s) 584
BisI GCNGC 6 cut(s) 6, 194, 197, 277, 426, 974
BlnI CCTAGG 1 cut(s) 977
BlsI GCNGC 6 cut(s) 7, 195, 198, 278, 427, 975
Bme1390I CCNGG 4 cut(s) 405, 578, 1081, 1335
Bme18I GGWCC 3 cut(s) 1125, 1274, 1385
BmgT120I GGNCC 4 cut(s) 1102, 1125, 1274, 1385
BmiI GGNNCC 5 cut(s) 48, 1077, 1126, 1341, 1386
BmrFI CCNGG 4 cut(s) 405, 578, 1081, 1335
BmsI GCATC 3 cut(s) 483, 631, 637
BpmI CTGGAG 3 cut(s) 10, 426, 1374
Bpu10I CCTNAGC 1 cut(s) 1410
BpuEI CTTGAG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 578
BsaBI GATNNNNATC 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 977, 1079, 1334
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 983
Bse1I ACTGG 3 cut(s) 48, 503, 1324
Bse21I CCTNAGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 917
Bse8I GATNNNNATC 1 cut(s) 80
BseBI CCWGG 3 cut(s) 405, 1081, 1335
BseDI CCNNGG 3 cut(s) 977, 1079, 1334
BseJI GATNNNNATC 1 cut(s) 80
BseLI CCNNNNNNNGG 2 cut(s) 58, 983
BseMI GCAATG 1 cut(s) 917
BseMII CTCAG 3 cut(s) 351, 445, 824
BseNI ACTGG 3 cut(s) 48, 503, 1324
BseRI GAGGAG 2 cut(s) 115, 708
BseSI GKGCMC 2 cut(s) 1080, 1342
BseXI GCAGC 5 cut(s) 180, 183, 288, 437, 985
BseYI CCCAGC 1 cut(s) 231
BshFI GGCC 2 cut(s) 760, 1103
BshNI GGYRCC 2 cut(s) 1075, 1339
BsiHKAI GWGCWC 3 cut(s) 108, 659, 1035
BsiSI CCGG 1 cut(s) 577
BslFI GGGAC 5 cut(s) 593, 827, 1138, 1338, 1398
BslI CCNNNNNNNGG 2 cut(s) 58, 983
BsmAI GTCTC 1 cut(s) 1408
BsmFI GGGAC 5 cut(s) 593, 827, 1138, 1338, 1398
BsmI GAATGC 1 cut(s) 1231
BsnI GGCC 2 cut(s) 760, 1103
Bsp1286I GDGCHC 5 cut(s) 108, 659, 1035, 1080, 1342
Bsp143I GATC 4 cut(s) 70, 493, 584, 772
BspANI GGCC 2 cut(s) 760, 1103
BspCNI CTCAG 3 cut(s) 350, 444, 825
BspHI TCATGA 1 cut(s) 723
BspLI GGNNCC 5 cut(s) 48, 1077, 1126, 1341, 1386
BspMAI CTGCAG 1 cut(s) 201
BspMI ACCTGC 1 cut(s) 1136
BspQI GCTCTTC 1 cut(s) 1167
BspT107I GGYRCC 2 cut(s) 1075, 1339
BsrDI GCAATG 1 cut(s) 917
BsrI ACTGG 3 cut(s) 48, 503, 1324
BssECI CCNNGG 3 cut(s) 977, 1079, 1334
BssMI GATC 4 cut(s) 70, 493, 584, 772
BssSI CACGAG 1 cut(s) 923
BssT1I CCWWGG 1 cut(s) 977
Bst2BI CACGAG 1 cut(s) 923
Bst2UI CCWGG 3 cut(s) 405, 1081, 1335
Bst4CI ACNGT 4 cut(s) 392, 487, 875, 1425
Bst6I CTCTTC 2 cut(s) 905, 1167
BstAPI GCANNNNNTGC 1 cut(s) 193
BstC8I GCNNGC 2 cut(s) 281, 472
BstDEI CTNAG 4 cut(s) 337, 431, 833, 1410
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 4 cut(s) 73, 496, 587, 775
BstMAI GTCTC 1 cut(s) 1408
BstMBI GATC 4 cut(s) 70, 493, 584, 772
BstMWI GCNNNNNNNGC 8 cut(s) 160, 193, 289, 318, 803, 826, 914, 1084
BstNI CCWGG 3 cut(s) 405, 1081, 1335
BstNSI RCATGY 1 cut(s) 820
BstSCI CCNGG 4 cut(s) 403, 576, 1079, 1333
BstSFI CTRYAG 2 cut(s) 197, 483
BstSLI GKGCMC 2 cut(s) 1080, 1342
BstV1I GCAGC 5 cut(s) 180, 183, 288, 437, 985
BstX2I RGATCY 1 cut(s) 584
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 1 cut(s) 584
Bsu36I CCTNAGG 1 cut(s) 337
BsuRI GGCC 2 cut(s) 760, 1103
BtsIMutI CAGTG 1 cut(s) 496
BveI ACCTGC 1 cut(s) 1136
Cac8I GCNNGC 2 cut(s) 281, 472
CciI TCATGA 1 cut(s) 723
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 4 cut(s) 1102, 1125, 1274, 1385
Csp6I GTAC 4 cut(s) 679, 1006, 1045, 1294
CviAII CATG 9 cut(s) 53, 599, 651, 713, 724, 817, 1009, 1108, 1271
CviQI GTAC 4 cut(s) 679, 1006, 1045, 1294
DdeI CTNAG 4 cut(s) 337, 431, 833, 1410
DpnI GATC 4 cut(s) 72, 495, 586, 774
DpnII GATC 4 cut(s) 70, 493, 584, 772
EaeI YGGCCR 1 cut(s) 758
Eam1104I CTCTTC 2 cut(s) 905, 1167
EarI CTCTTC 2 cut(s) 905, 1167
Eco130I CCWWGG 1 cut(s) 977
Eco47I GGWCC 3 cut(s) 1125, 1274, 1385
Eco57I CTGAAG 1 cut(s) 1285
Eco81I CCTNAGG 1 cut(s) 337
EcoO109I RGGNCCY 3 cut(s) 1102, 1125, 1385
EcoRII CCWGG 3 cut(s) 403, 1079, 1333
EcoT14I CCWWGG 1 cut(s) 977
EcoT22I ATGCAT 2 cut(s) 652, 1231
ErhI CCWWGG 1 cut(s) 977
FaeI CATG 9 cut(s) 56, 602, 654, 716, 727, 820, 1012, 1111, 1274
FalI AAGNNNNNCTT 2 cut(s) 1175, 1207
FaqI GGGAC 5 cut(s) 593, 827, 1138, 1338, 1398
FatI CATG 9 cut(s) 52, 598, 650, 712, 723, 816, 1008, 1107, 1270
FauNDI CATATG 1 cut(s) 457
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 6 cut(s) 6, 194, 197, 277, 426, 974
Fsp4HI GCNGC 6 cut(s) 6, 194, 197, 277, 426, 974
FspBI CTAG 2 cut(s) 588, 978
GlaI GCGC 1 cut(s) 155
GluI GCNGC 6 cut(s) 6, 194, 197, 277, 426, 974
GsaI CCCAGC 1 cut(s) 235
GsuI CTGGAG 3 cut(s) 10, 426, 1374
HaeIII GGCC 2 cut(s) 760, 1103
HapII CCGG 1 cut(s) 577
HhaI GCGC 1 cut(s) 156
Hin1II CATG 9 cut(s) 56, 602, 654, 716, 727, 820, 1012, 1111, 1274
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HindIII AAGCTT 2 cut(s) 827, 1249
HinfI GANTC 7 cut(s) 63, 76, 122, 396, 709, 895, 1162
HpaII CCGG 1 cut(s) 577
HphI GGTGA 2 cut(s) 998, 1234
Hpy166II GTNNAC 4 cut(s) 121, 287, 740, 1369
Hpy188I TCNGA 5 cut(s) 75, 130, 661, 834, 1304
Hpy188III TCNNGA 9 cut(s) 250, 339, 368, 532, 724, 776, 1123, 1169, 1391
Hpy8I GTNNAC 4 cut(s) 121, 287, 740, 1369
Hpy99I CGWCG 1 cut(s) 1289
HpyAV CCTTC 4 cut(s) 683, 699, 947, 1283
HpyCH4III ACNGT 4 cut(s) 392, 487, 875, 1425
HpyCH4IV ACGT 2 cut(s) 858, 1284
HpyF10VI GCNNNNNNNGC 8 cut(s) 160, 193, 289, 318, 803, 826, 914, 1084
HpyF3I CTNAG 4 cut(s) 337, 431, 833, 1410
HpySE526I ACGT 2 cut(s) 858, 1284
Hsp92II CATG 9 cut(s) 56, 602, 654, 716, 727, 820, 1012, 1111, 1274
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 4 cut(s) 70, 493, 584, 772
LguI GCTCTTC 1 cut(s) 1167
LmnI GCTCC 5 cut(s) 103, 297, 579, 662, 884
Lsp1109I GCAGC 5 cut(s) 180, 183, 288, 437, 985
LweI GCATC 3 cut(s) 483, 631, 637
MaeI CTAG 2 cut(s) 588, 978
MaeII ACGT 2 cut(s) 858, 1284
MaeIII GTNAC 4 cut(s) 11, 261, 869, 986
MalI GATC 4 cut(s) 72, 495, 586, 774
MboI GATC 4 cut(s) 70, 493, 584, 772
MboII GAAGA 7 cut(s) 503, 514, 757, 892, 1157, 1184, 1291
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 5 cut(s) 108, 659, 1035, 1080, 1342
MlsI TGGCCA 1 cut(s) 760
MluCI AATT 5 cut(s) 19, 177, 909, 995, 1186
MluNI TGGCCA 1 cut(s) 760
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 684, 921
MnlI CCTC 8 cut(s) 136, 346, 617, 686, 919, 1115, 1142, 1398
Mox20I TGGCCA 1 cut(s) 760
Mph1103I ATGCAT 2 cut(s) 652, 1231
MroXI GAANNNNTTC 1 cut(s) 1190
MscI TGGCCA 1 cut(s) 760
MseI TTAA 3 cut(s) 267, 699, 1373
Msp20I TGGCCA 1 cut(s) 760
MspI CCGG 1 cut(s) 577
MspR9I CCNGG 4 cut(s) 405, 578, 1081, 1335
MunI CAATTG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 1231
MvaI CCWGG 3 cut(s) 405, 1081, 1335
MwoI GCNNNNNNNGC 8 cut(s) 160, 193, 289, 318, 803, 826, 914, 1084
NciI CCSGG 1 cut(s) 578
NdeI CATATG 1 cut(s) 457
NdeII GATC 4 cut(s) 70, 493, 584, 772
NlaIII CATG 9 cut(s) 56, 602, 654, 716, 727, 820, 1012, 1111, 1274
NlaIV GGNNCC 5 cut(s) 48, 1077, 1126, 1341, 1386
NmuCI GTSAC 3 cut(s) 261, 869, 986
NsiI ATGCAT 2 cut(s) 652, 1231
NspI RCATGY 1 cut(s) 820
PagI TCATGA 1 cut(s) 723
PciSI GCTCTTC 1 cut(s) 1167
PcsI WCGNNNNNNNCGW 1 cut(s) 930
PctI GAATGC 1 cut(s) 1231
PdmI GAANNNNTTC 1 cut(s) 1190
PfeI GAWTC 6 cut(s) 63, 76, 396, 709, 895, 1162
PflFI GACNNNGTC 1 cut(s) 1283
PflMI CCANNNNNTGG 1 cut(s) 58
PfoI TCCNGGA 1 cut(s) 576
PkrI GCNGC 6 cut(s) 7, 195, 198, 278, 427, 975
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PpuMI RGGWCCY 2 cut(s) 1125, 1385
Psp5II RGGWCCY 2 cut(s) 1125, 1385
Psp6I CCWGG 3 cut(s) 403, 1079, 1333
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 3 cut(s) 403, 1079, 1333
PspN4I GGNNCC 5 cut(s) 48, 1077, 1126, 1341, 1386
PspPI GGNCC 4 cut(s) 1102, 1125, 1274, 1385
PspPPI RGGWCCY 2 cut(s) 1125, 1385
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 584
PsyI GACNNNGTC 1 cut(s) 1283
RsaI GTAC 4 cut(s) 680, 1007, 1046, 1295
RsaNI GTAC 4 cut(s) 679, 1006, 1045, 1294
SapI GCTCTTC 1 cut(s) 1167
SaqAI TTAA 3 cut(s) 267, 699, 1373
SatI GCNGC 6 cut(s) 6, 194, 197, 277, 426, 974
Sau3AI GATC 4 cut(s) 70, 493, 584, 772
Sau96I GGNCC 4 cut(s) 1102, 1125, 1274, 1385
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 4 cut(s) 405, 578, 1081, 1335
SduI GDGCHC 5 cut(s) 108, 659, 1035, 1080, 1342
SfaNI GCATC 3 cut(s) 483, 631, 637
SfcI CTRYAG 2 cut(s) 197, 483
SinI GGWCC 3 cut(s) 1125, 1274, 1385
SmlI CTYRAG 1 cut(s) 561
SmoI CTYRAG 1 cut(s) 561
Sse9I AATT 5 cut(s) 19, 177, 909, 995, 1186
SspMI CTAG 2 cut(s) 588, 978
StyD4I CCNGG 4 cut(s) 403, 576, 1079, 1333
StyI CCWWGG 1 cut(s) 977
TaaI ACNGT 4 cut(s) 392, 487, 875, 1425
TaiI ACGT 2 cut(s) 861, 1287
TaqI TCGA 4 cut(s) 209, 240, 489, 1287
TasI AATT 5 cut(s) 19, 177, 909, 995, 1186
TatI WGTACW 1 cut(s) 1005
TfiI GAWTC 6 cut(s) 63, 76, 396, 709, 895, 1162
Tru1I TTAA 3 cut(s) 267, 699, 1373
Tru9I TTAA 3 cut(s) 267, 699, 1373
TscAI CASTG 1 cut(s) 503
TseFI GTSAC 3 cut(s) 261, 869, 986
TseI GCWGC 6 cut(s) 5, 193, 196, 276, 425, 973
Tsp45I GTSAC 3 cut(s) 261, 869, 986
TspDTI ATGAA 6 cut(s) 444, 701, 712, 740, 1032, 1167
TspRI CASTG 1 cut(s) 503
Tth111I GACNNNGTC 1 cut(s) 1283
Van91I CCANNNNNTGG 1 cut(s) 58
VpaK11BI GGWCC 3 cut(s) 1125, 1274, 1385
XapI RAATTY 2 cut(s) 19, 995
XceI RCATGY 1 cut(s) 820
XmaJI CCTAGG 1 cut(s) 977
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 1190
XspI CTAG 2 cut(s) 588, 978
Zsp2I ATGCAT 2 cut(s) 652, 1231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.