FvH4_1g14260

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012563
Physical Location & Seq
Forward (+)
7908995 .. 7911146
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14260.t1

Sequence Viewer

Length: 1863 bp
ATGATGGAGTACTACTCCATACTTGTTTTCTTGCTTTTCTTGCTGTTCACTCCAAAGTTCAAAGCTCAACAAAGCTATGCCGGTAACTCAGTTCTGGACTGTAATAGCACTGATGATGCAGGGCCATCCTCGGCTTTTCTTTACACCTGCAATGGTCAGAAGCAGTCCTGCAAGTCTTTCTTGATCTTCAAGTCTCAACCTCCTTACAATTCACTCTCCGCCATCTCAAACCTCACATCTTCAGACCCATCTGAGCTTGCTCGAATCAACATCATCTCCGCTAACACACAGTTGTTGCCTACAAGCAAAGAGGTGATTGTTCCTGTGGTCTGTTCTTGCTCAGGTCAGTACTATGAGGCCAAAACCTCTTTTCTAGTTCCCAGCATTGATGACACCTATTTTACAATAGCAAACAACACATATCAAGGTTTGTCTACTTGTGATGCTCTCATGAGCCATAACAATTACAGTGCAACTAGTGTGGAACCTGGTTTGAAGTTACAGGTACCACTTAGGTGTGCTTGTCCAACAAGAAACCAAACTTTGAATGGTACTAAATTTTTGTTAACCTACTTGGTTAGCTGGGATGACTCTGTCTCTGAAGTTAGTAAGAGATTTAATGTCAGTGAAAAGAGTGTCCTTCAGGCCAATGGGTTTACAGAAGACGATCCTACTTTGTTTCCCTTTACAACAATTCTCATTCCACTTCCAGTAGAGCCTTCAAGCTTTCAAACCCAAATCCAGTATCCCCCGCCACTGATTTCTCCTTCGGTTAGGCCTATTCATGGAAAAACAAGGTTAAGGAAAGTATATGTTTGGATAGGGATTGGAGTTTTTGTAGTAGTCCTTTGCATGAGTCTCTTTGTGGCCCTCTTGTATCATCGGAAAAGGGGTTCTCCAAAGATTATTGCTGAAGGAAAATCCAAAAAGGAATTGGATTTGCAAGAAGAACTGTATGTTTCCATTGCTAAAGCTGATGCTGGTTTGAAACTCTATAGATATGAAGAACTGGAAAGTGCTACAGATGATTTCAGCAGCAAGAACTGGTTAGGTGGCTCTGTTTATAGTGGCTTTCTCAGTGGAAATGTGCTAGTAGCCATAAAAAAGATGACCAAAGATGTGAGTGTGGAGGTGAAGTTACTGCAAAAGATCAACCACTTCAATCTGATAAGTCTTTTGGGCGCGTGCCAACACTGTAAGGATTTCTACCTTGTCTATGAGTTCATGGAGAATGGTTCATTAAGAGATTGGCTAAGTTGTCCACAAGTTTTGGGATGGAAGCACAGGGTTCAAATTGCTTTGGACATTGCCAATGGGCTTCACTATCTTCACAATTTCACCAGTCCTGCCTATGTGCACAAGGATATTAAAAGTATCAATATTCTTCTCAATAAAGATTTAAGGGCAAAGATTGCCAATTTCAGTCTTGCAAGATCAGCAGAGAAGGAAACTTATGGCTTTTCATCTCGGAAGTTTGCACATGTATCAAAAGGCTACATGGCACCAGAATATATAGAATATGGGTTGGTGACACCTGCAATGGATGTATATGCTTTTGGGGTTGTCATGCTGGAACTAGTAACAGGAAAAGAAGCTGTCTTCACTGAGGATGGAGTAGAAGCACTTCTTTCAGAAGCTGTGTTTTTAGTAATGGAAGGAGGAGATGGAGAAGTTGCCCTTGTTGGAATAGTAGATCCCAATTTGAATGTAGAGCACTTGATGGAGTTTGCAGTAAGATTGATAAAGTTAAGTCTAGCCTGCTTGGCTGAAGAACCAGAAGATAGACCTAGAATGCCTCAAGTACTCTCATCTGTGCTGAAAATACAATTGGATGCAAATAGGAAAATACAGAACTTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

621

Amino Acids

69.08

Weight (kDa)

6.3

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM1_NFP_LYK PF23446 56 - 113 1.7e-15 NFP/LYK LysM1 domain
LysM2_CERK1_LYK3_4_5 PF23472 117 - 172 9e-24 LYK3/4/5 LysM2 domain
LysM3_LYK4_5 PF23473 187 - 236 1.3e-20 LYK4/5 LysM3 domain
PK_Tyr_Ser-Thr PF07714 352 - 603 2.3e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 354 - 603 8.3e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 155, 1543
Acc36I ACCTGC 2 cut(s) 155, 1543
Acc65I GGTACC 1 cut(s) 505
AccB1I GGYRCC 2 cut(s) 505, 1501
AccI GTMKAC 1 cut(s) 434
AccII CGCG 1 cut(s) 1184
AciI CCGC 3 cut(s) 219, 279, 752
AclWI GGATC 2 cut(s) 662, 1688
AcsI RAATTY 1 cut(s) 557
AcuI CTGAAG 5 cut(s) 225, 621, 626, 933, 1788
AfaI GTAC 5 cut(s) 11, 350, 507, 553, 1803
AfiI CCNNNNNNNGG 1 cut(s) 785
AflIII ACRYGT 1 cut(s) 1480
AhlI ACTAGT 2 cut(s) 476, 1576
AjnI CCWGG 1 cut(s) 487
AjuI GAANNNNNNNTTGG 2 cut(s) 1811, 1843
AleI CACNNNNGTG 1 cut(s) 514
AloI GAACNNNNNNTCC 2 cut(s) 877, 909
AluBI AGCT 8 cut(s) 65, 75, 256, 582, 726, 974, 1595, 1637
AluI AGCT 8 cut(s) 65, 75, 256, 582, 726, 974, 1595, 1637
Alw21I GWGCWC 2 cut(s) 1359, 1716
Alw26I GTCTC 3 cut(s) 198, 601, 863
Alw44I GTGCAC 1 cut(s) 1355
AlwI GGATC 2 cut(s) 662, 1688
AlwNI CAGNNNCTG 1 cut(s) 1637
AoxI GGCC 5 cut(s) 122, 357, 645, 776, 867
ApaLI GTGCAC 1 cut(s) 1355
ApeKI GCWGC 1 cut(s) 1035
ApoI RAATTY 1 cut(s) 557
Asp700I GAANNNNTTC 1 cut(s) 1623
Asp718I GGTACC 1 cut(s) 505
AspLEI GCGC 1 cut(s) 1184
AspS9I GGNCC 2 cut(s) 122, 868
AsuHPI GGTGA 4 cut(s) 325, 1144, 1330, 1540
BaeGI GKGCMC 1 cut(s) 1359
BanI GGYRCC 2 cut(s) 505, 1501
BarI GAAGNNNNNNTAC 2 cut(s) 1608, 1640
BbsI GAAGAC 2 cut(s) 669, 1591
Bbv12I GWGCWC 2 cut(s) 1359, 1716
BbvI GCAGC 1 cut(s) 1047
BccI CCATC 7 cut(s) 133, 230, 256, 1269, 1604, 1658, 1714
BciT130I CCWGG 1 cut(s) 489
BciVI GTATCC 1 cut(s) 756
BcoDI GTCTC 3 cut(s) 198, 601, 863
BcuI ACTAGT 2 cut(s) 476, 1576
BfaI CTAG 6 cut(s) 374, 477, 1091, 1577, 1754, 1788
BfmI CTRYAG 2 cut(s) 994, 1020
BfuAI ACCTGC 2 cut(s) 155, 1543
BfuI GTATCC 1 cut(s) 756
BglI GCCNNNNNGGC 1 cut(s) 1763
BisI GCNGC 1 cut(s) 1036
BlsI GCNGC 1 cut(s) 1037
BmcAI AGTACT 3 cut(s) 11, 350, 1803
Bme1390I CCNGG 1 cut(s) 489
BmgT120I GGNCC 2 cut(s) 122, 868
BmiI GGNNCC 3 cut(s) 486, 507, 1503
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 4 cut(s) 106, 433, 967, 1822
BpiI GAAGAC 2 cut(s) 669, 1591
BplI GAGNNNNNCTC 1 cut(s) 31
Bpu10I CCTNAGC 1 cut(s) 340
BpuEI CTTGAG 1 cut(s) 1782
BsaJI CCNNGG 1 cut(s) 129
BsaXI ACNNNNNCTCC 6 cut(s) 260, 290, 822, 852, 1121, 1151
Bsc4I CCNNNNNNNGG 1 cut(s) 785
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 5 cut(s) 710, 742, 1014, 1049, 1341
Bse3DI GCAATG 4 cut(s) 157, 963, 1305, 1545
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 6 cut(s) 125, 592, 1280, 1549, 1615, 1837
BseLI CCNNNNNNNGG 1 cut(s) 785
BseMI GCAATG 4 cut(s) 157, 963, 1305, 1545
BseMII CTCAG 5 cut(s) 102, 243, 354, 1090, 1596
BseNI ACTGG 5 cut(s) 710, 742, 1014, 1049, 1341
BseRI GAGGAG 1 cut(s) 1674
BseSI GKGCMC 1 cut(s) 1359
BseXI GCAGC 1 cut(s) 1047
BseYI CCCAGC 2 cut(s) 380, 582
Bsh1236I CGCG 1 cut(s) 1184
BshFI GGCC 5 cut(s) 124, 359, 647, 778, 869
BshNI GGYRCC 2 cut(s) 505, 1501
BsiHKAI GWGCWC 2 cut(s) 1359, 1716
BsiSI CCGG 1 cut(s) 81
BslI CCNNNNNNNGG 1 cut(s) 785
BsmAI GTCTC 3 cut(s) 198, 601, 863
BsmI GAATGC 1 cut(s) 1797
BsnI GGCC 5 cut(s) 124, 359, 647, 778, 869
Bsp1286I GDGCHC 2 cut(s) 1359, 1716
Bsp143I GATC 5 cut(s) 183, 667, 1149, 1433, 1693
BspACI CCGC 3 cut(s) 219, 279, 752
BspANI GGCC 5 cut(s) 124, 359, 647, 778, 869
BspCNI CTCAG 5 cut(s) 101, 244, 353, 1089, 1597
BspFNI CGCG 1 cut(s) 1184
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 3 cut(s) 486, 507, 1503
BspMI ACCTGC 2 cut(s) 155, 1543
BspPI GGATC 2 cut(s) 662, 1688
BspT107I GGYRCC 2 cut(s) 505, 1501
BsrDI GCAATG 4 cut(s) 157, 963, 1305, 1545
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 5 cut(s) 710, 742, 1014, 1049, 1341
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 5 cut(s) 183, 667, 1149, 1433, 1693
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 5 cut(s) 101, 291, 470, 954, 1196
BstAPI GCANNNNNTGC 1 cut(s) 1412
BstC8I GCNNGC 3 cut(s) 258, 1186, 1759
BstDEI CTNAG 7 cut(s) 88, 252, 340, 512, 1076, 1253, 1605
BstF5I GGATG 6 cut(s) 125, 592, 1280, 1549, 1615, 1837
BstFNI CGCG 1 cut(s) 1184
BstHHI GCGC 1 cut(s) 1184
BstKTI GATC 5 cut(s) 186, 670, 1152, 1436, 1696
BstMAI GTCTC 3 cut(s) 198, 601, 863
BstMBI GATC 5 cut(s) 183, 667, 1149, 1433, 1693
BstMWI GCNNNNNNNGC 4 cut(s) 40, 1412, 1436, 1763
BstNI CCWGG 1 cut(s) 489
BstNSI RCATGY 1 cut(s) 1484
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 2 cut(s) 994, 1020
BstSLI GKGCMC 1 cut(s) 1359
BstUI CGCG 1 cut(s) 1184
BstV1I GCAGC 1 cut(s) 1047
BstV2I GAAGAC 2 cut(s) 669, 1591
BstX2I RGATCY 1 cut(s) 1693
BstYI RGATCY 1 cut(s) 1693
BsuI GTATCC 1 cut(s) 756
BsuRI GGCC 5 cut(s) 124, 359, 647, 778, 869
BtsCI GGATG 6 cut(s) 125, 592, 1280, 1549, 1615, 1837
BtsIMutI CAGTG 7 cut(s) 108, 475, 631, 755, 1084, 1192, 1602
BveI ACCTGC 2 cut(s) 155, 1543
Cac8I GCNNGC 3 cut(s) 258, 1186, 1759
CaiI CAGNNNCTG 1 cut(s) 1637
CciI TCATGA 1 cut(s) 450
CfoI GCGC 1 cut(s) 1184
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 122, 868
CsiI ACCWGGT 1 cut(s) 487
Csp6I GTAC 5 cut(s) 10, 349, 506, 552, 1802
CspCI CAANNNNNGTGG 4 cut(s) 462, 497, 1251, 1286
CviAII CATG 7 cut(s) 451, 785, 853, 1225, 1481, 1498, 1567
CviQI GTAC 5 cut(s) 10, 349, 506, 552, 1802
DdeI CTNAG 7 cut(s) 88, 252, 340, 512, 1076, 1253, 1605
DpnI GATC 5 cut(s) 185, 669, 1151, 1435, 1695
DpnII GATC 5 cut(s) 183, 667, 1149, 1433, 1693
EciI GGCGGA 1 cut(s) 208
Eco147I AGGCCT 1 cut(s) 778
Eco57I CTGAAG 5 cut(s) 225, 621, 626, 933, 1788
EcoRII CCWGG 1 cut(s) 487
FaeI CATG 7 cut(s) 454, 788, 856, 1228, 1484, 1501, 1570
FalI AAGNNNNNCTT 6 cut(s) 164, 196, 1611, 1643, 1662, 1694
FatI CATG 7 cut(s) 450, 784, 852, 1224, 1480, 1497, 1566
FauI CCCGC 1 cut(s) 759
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 1 cut(s) 1036
FokI GGATG 6 cut(s) 112, 599, 1287, 1556, 1622, 1844
Fsp4HI GCNGC 1 cut(s) 1036
FspBI CTAG 6 cut(s) 374, 477, 1091, 1577, 1754, 1788
GlaI GCGC 1 cut(s) 1183
GluI GCNGC 1 cut(s) 1036
GsaI CCCAGC 2 cut(s) 384, 586
HaeIII GGCC 5 cut(s) 124, 359, 647, 778, 869
HapII CCGG 1 cut(s) 81
HhaI GCGC 1 cut(s) 1184
Hin1II CATG 7 cut(s) 454, 788, 856, 1228, 1484, 1501, 1570
Hin6I GCGC 1 cut(s) 1182
HinP1I GCGC 1 cut(s) 1182
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HindIII AAGCTT 1 cut(s) 724
HinfI GANTC 3 cut(s) 264, 590, 856
HpaI GTTAAC 1 cut(s) 567
HpaII CCGG 1 cut(s) 81
HphI GGTGA 4 cut(s) 325, 1144, 1330, 1540
Hpy166II GTNNAC 6 cut(s) 48, 435, 567, 657, 1262, 1357
Hpy188I TCNGA 8 cut(s) 159, 244, 253, 601, 885, 1167, 1470, 1633
Hpy188III TCNNGA 3 cut(s) 95, 181, 451
Hpy8I GTNNAC 6 cut(s) 48, 435, 567, 657, 1262, 1357
HpyAV CCTTC 6 cut(s) 650, 729, 777, 908, 1438, 1649
HpyCH4III ACNGT 5 cut(s) 101, 291, 470, 954, 1196
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 1412, 1436, 1763
HpyF3I CTNAG 7 cut(s) 88, 252, 340, 512, 1076, 1253, 1605
Hsp92II CATG 7 cut(s) 454, 788, 856, 1228, 1484, 1501, 1570
HspAI GCGC 1 cut(s) 1182
KpnI GGTACC 1 cut(s) 509
KspAI GTTAAC 1 cut(s) 567
Kzo9I GATC 5 cut(s) 183, 667, 1149, 1433, 1693
Lsp1109I GCAGC 1 cut(s) 1047
LweI GCATC 4 cut(s) 106, 433, 967, 1822
MabI ACCWGGT 1 cut(s) 487
MaeI CTAG 6 cut(s) 374, 477, 1091, 1577, 1754, 1788
MaeIII GTNAC 5 cut(s) 83, 498, 1137, 1528, 1579
MalI GATC 5 cut(s) 185, 669, 1151, 1435, 1695
MboI GATC 5 cut(s) 183, 667, 1149, 1433, 1693
MfeI CAATTG 1 cut(s) 1826
MflI RGATCY 1 cut(s) 1693
MhlI GDGCHC 2 cut(s) 1359, 1716
MlyI GAGTC 2 cut(s) 584, 865
MmeI TCCRAC 2 cut(s) 551, 1663
MroXI GAANNNNTTC 1 cut(s) 1623
MseI TTAA 7 cut(s) 566, 618, 800, 1241, 1368, 1400, 1748
MslI CAYNNNNRTG 1 cut(s) 514
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 489
MunI CAATTG 1 cut(s) 1826
Mva1269I GAATGC 1 cut(s) 1797
MvaI CCWGG 1 cut(s) 489
MvnI CGCG 1 cut(s) 1184
MwoI GCNNNNNNNGC 4 cut(s) 40, 1412, 1436, 1763
NdeII GATC 5 cut(s) 183, 667, 1149, 1433, 1693
NlaIII CATG 7 cut(s) 454, 788, 856, 1228, 1484, 1501, 1570
NlaIV GGNNCC 3 cut(s) 486, 507, 1503
NmeAIII GCCGAG 1 cut(s) 110
NmuCI GTSAC 1 cut(s) 1528
NspI RCATGY 1 cut(s) 1484
OliI CACNNNNGTG 1 cut(s) 514
PagI TCATGA 1 cut(s) 450
PaqCI CACCTGC 2 cut(s) 155, 1543
PceI AGGCCT 1 cut(s) 778
PciI ACATGT 1 cut(s) 1480
PctI GAATGC 1 cut(s) 1797
PdmI GAANNNNTTC 1 cut(s) 1623
PfeI GAWTC 1 cut(s) 264
PflFI GACNNNGTC 1 cut(s) 593
PkrI GCNGC 1 cut(s) 1037
PleI GAGTC 2 cut(s) 584, 864
PpsI GAGTC 2 cut(s) 584, 864
PscI ACATGT 1 cut(s) 1480
Psp6I CCWGG 1 cut(s) 487
PspFI CCCAGC 2 cut(s) 380, 582
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 3 cut(s) 486, 507, 1503
PspPI GGNCC 2 cut(s) 122, 868
PstNI CAGNNNCTG 1 cut(s) 1637
PsuI RGATCY 1 cut(s) 1693
PsyI GACNNNGTC 1 cut(s) 593
RsaI GTAC 5 cut(s) 11, 350, 507, 553, 1803
RsaNI GTAC 5 cut(s) 10, 349, 506, 552, 1802
RseI CAYNNNNRTG 1 cut(s) 514
SaqAI TTAA 7 cut(s) 566, 618, 800, 1241, 1368, 1400, 1748
SatI GCNGC 1 cut(s) 1036
Sau3AI GATC 5 cut(s) 183, 667, 1149, 1433, 1693
Sau96I GGNCC 2 cut(s) 122, 868
ScaI AGTACT 3 cut(s) 11, 350, 1803
SchI GAGTC 2 cut(s) 584, 865
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 2 cut(s) 1359, 1716
SexAI ACCWGGT 1 cut(s) 487
SfaNI GCATC 4 cut(s) 106, 433, 967, 1822
SfcI CTRYAG 2 cut(s) 994, 1020
SmiMI CAYNNNNRTG 1 cut(s) 514
SmlI CTYRAG 1 cut(s) 1797
SmoI CTYRAG 1 cut(s) 1797
SpeI ACTAGT 2 cut(s) 476, 1576
SseBI AGGCCT 1 cut(s) 778
SsiI CCGC 3 cut(s) 219, 279, 752
SspI AATATT 1 cut(s) 1381
SspMI CTAG 6 cut(s) 374, 477, 1091, 1577, 1754, 1788
StuI AGGCCT 1 cut(s) 778
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 5 cut(s) 101, 291, 470, 954, 1196
TaqI TCGA 1 cut(s) 262
TatI WGTACW 3 cut(s) 9, 348, 1801
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 7 cut(s) 566, 618, 800, 1241, 1368, 1400, 1748
Tru9I TTAA 7 cut(s) 566, 618, 800, 1241, 1368, 1400, 1748
TscAI CASTG 7 cut(s) 115, 475, 631, 762, 1084, 1199, 1609
TseFI GTSAC 1 cut(s) 1528
TseI GCWGC 1 cut(s) 1035
Tsp45I GTSAC 1 cut(s) 1528
TspDTI ATGAA 5 cut(s) 773, 1017, 1213, 1227, 1452
TspRI CASTG 7 cut(s) 115, 475, 631, 762, 1084, 1199, 1609
Tth111I GACNNNGTC 1 cut(s) 593
VneI GTGCAC 1 cut(s) 1355
XapI RAATTY 1 cut(s) 557
XceI RCATGY 1 cut(s) 1484
XcmI CCANNNNNNNNNTGG 2 cut(s) 545, 931
XmiI GTMKAC 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 1623
XspI CTAG 6 cut(s) 374, 477, 1091, 1577, 1754, 1788
ZrmI AGTACT 3 cut(s) 11, 350, 1803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.