Rroxscaffold_2G00140090

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012563
Physical Location & Seq
Reverse (-)
78388071 .. 78392313
4243 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140090.1

Sequence Viewer

Length: 1821 bp
ATGGCTGCAATTGATGTTCTCAAGTATGCTCATAGTCCCGCACACAAAGCCATAGTCACCAAGGACTATGCCGGTCTCGAGAGGATTCTTGCAGCTCTTCCGCGACTTTCTAATCTCGCCGAGATTCAAACCGAGTCGGCTTCATTAGCTGAAGAATTCTTGGTGATGGGTCCGACGATGGCAAGGCCCGGCATTGATGTGACTCGTGCGGTTCTCTTACCTCGCCTGACTTGGAGGCGGCAGGAGAAAACGAAACGGTTGGATCATGGAAGGCTAAGTGAGGAACTTGATGATATTTTGACAGAGGATGAGAGGAAGCAACTTGAAGTTGCACCGAAACTAGATTCATCCGAATCGACCAACGAGAGTGGTGATGGAATTATCGAGCATCGTCATAGTTGTTATGAGTCAAGAGAAATTCCTATTGAGGATGTAAGCAATTGTAGAAATGGAGAGACCAAGCAAGAAAAGAAAGGATGGTTTGGTGGATGGAGGAAATGGGATACCAAAAATGAAGGACATAAAAAGACCGCCCCACCAAGAAGTTCTCTTTGTGTGGATGAGAAGAGTGAGAAAGTAGGGTTCGAGGACGGAGATGTCCGAAGTTCGACGGTTAAAGATAACCGAAGACCCAGTGGGGTGTTGGGATTGAGGATTTTGAATGTATTTCCTCGTGATTCCCGTCAGTACTATGAGGCCAAAACCTCTATTTTAGTTCCGAGCATCGATGACACCTATTTTACAATAGCAAACAGCACATATCAAGCTTTGTCTACTTGTGATGCTCTGATGAGCCACAACAATTACAGAGAATTTAGCGTGAAACCTGGTTTGAAGTTACAAGTACCACTTAGCTGGGATGACTCTGTCTCCGTAGTTAGTGAGAGATTTAATGTCAGTGAAAAGAGCGTCCTTCATGCCAATGGGTTTACAGAAGATGATCCTACTTTATATGAAGAATTGGAAAGTGCTACAGAGGATTTCAGCAACAAGAACTGGTTGGGTGGCTCTGTTTATAGTGGTTTTCTCAGTGGAAATGTGCTAGTAGCCATAAAAAAGATGACCAAAGATGTGAGTGAGGAGGTGAAGTTACTGCAAAAGATCAACCACTTCAATCTAATAAGTCTTTTGGGTGCGTGCGAACACCGTAAGGATTTCTACCTTGTTTATGAGTTCATGGAGAATGGTTCATTAAGAGATTGGCTAAGTTGTCCCCAAGTTTTGGGATGGAAGCACAGGGTTCAGATTGCTTTGGACATTGCCAATGGGCTTCACTATCTTCACAACTTCACCAGTCCTGCCTATGTGCACAAGGATATCAAAAGTACCAATGTTCTTCTCAATAGAGATTTAAGGGCAAAGATTGGAAATTTTAGTCTCGCAAGATCAGCGGAGAAGGAAACATATGGCTTTTCATCTCGGAAGTTTGCACAGGTATCAAAAGGCTACATGGCACCAGAATATATAGAATATGGCTTGGTGGCACCAGCAGTGGATGTATATGCTTTTGGGGTTGTTATGCTGGAATTAGTCACAGGAAAAGAAGCTGTTTTTACCGAGGATGGAGTTGAAGTATTTCTTTCAGAAGCTATATTTTCAGTAATGGAAGGAGGAAATGGAGAAGTTGAGCCTGTTGGACTAGTAGATCCCAATTTGAATGTAGAGCACTTGATGGAGTTTGCAGTGAGATTGATAAAGTTAAGTCTAGCCTGCTTGGCTGAAGAACCAGAAGACAGACCCAGAATGCCTCAAGTACTCTCATCTGTGCTGAAAATACAATTGGATGCAAACAGGAAAATGCAGAACTTCCATGTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

606

Amino Acids

68.21

Weight (kDa)

5.65

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM2_CERK1_LYK3_4_5 PF23472 230 - 284 1.3e-20 LYK3/4/5 LysM2 domain
LysM3_LYK4_5 PF23473 285 - 314 3.3e-07 LYK4/5 LysM3 domain
PK_Tyr_Ser-Thr PF07714 336 - 587 2.3e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 344 - 587 1.3e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 596
AccB1I GGYRCC 2 cut(s) 1453, 1483
AccB7I CCANNNNNTGG 1 cut(s) 1222
AccI GTMKAC 1 cut(s) 773
AccII CGCG 1 cut(s) 103
AciI CCGC 6 cut(s) 39, 101, 209, 238, 531, 1391
AclWI GGATC 3 cut(s) 270, 935, 1640
AcsI RAATTY 4 cut(s) 155, 417, 812, 1369
AcuI CTGAAG 2 cut(s) 171, 1740
AfaI GTAC 4 cut(s) 689, 846, 1327, 1755
AfiI CCNNNNNNNGG 1 cut(s) 1222
AgsI TTSAA 7 cut(s) 128, 326, 661, 835, 1114, 1571, 1657
AhlI ACTAGT 1 cut(s) 1639
AjnI CCWGG 1 cut(s) 826
AjuI GAANNNNNNNTTGG 2 cut(s) 1763, 1795
AluBI AGCT 6 cut(s) 95, 149, 767, 855, 1547, 1589
AluI AGCT 6 cut(s) 95, 149, 767, 855, 1547, 1589
Alw21I GWGCWC 2 cut(s) 1311, 1668
Alw26I GTCTC 4 cut(s) 80, 449, 874, 1382
Alw44I GTGCAC 1 cut(s) 1307
AlwI GGATC 3 cut(s) 270, 935, 1640
Ama87I CYCGRG 1 cut(s) 77
AoxI GGCC 2 cut(s) 185, 696
ApaLI GTGCAC 1 cut(s) 1307
ApeKI GCWGC 2 cut(s) 5, 92
ApoI RAATTY 4 cut(s) 155, 417, 812, 1369
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
Asp700I GAANNNNTTC 1 cut(s) 1575
AspS9I GGNCC 2 cut(s) 170, 186
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 5 cut(s) 49, 175, 383, 1096, 1282
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 1311
BanI GGYRCC 2 cut(s) 1453, 1483
BauI CACGAG 2 cut(s) 204, 672
BbsI GAAGAC 2 cut(s) 634, 1737
Bbv12I GWGCWC 2 cut(s) 1311, 1668
BbvI GCAGC 1 cut(s) 104
BccI CCATC 8 cut(s) 160, 172, 368, 471, 483, 1221, 1556, 1666
BciT130I CCWGG 1 cut(s) 828
BciVI GTATCC 1 cut(s) 496
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 4 cut(s) 80, 449, 874, 1382
BcuI ACTAGT 1 cut(s) 1639
BfaI CTAG 4 cut(s) 341, 1043, 1640, 1706
BfmI CTRYAG 1 cut(s) 972
BfuI GTATCC 1 cut(s) 496
BglI GCCNNNNNGGC 1 cut(s) 1715
BisI GCNGC 3 cut(s) 6, 93, 239
BlsI GCNGC 3 cut(s) 7, 94, 240
BmcAI AGTACT 2 cut(s) 689, 1755
Bme1390I CCNGG 2 cut(s) 189, 828
Bme18I GGWCC 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 2 cut(s) 170, 186
BmiI GGNNCC 3 cut(s) 171, 1455, 1485
BmrFI CCNGG 2 cut(s) 189, 828
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 4 cut(s) 397, 732, 772, 1774
BmuI ACTGGG 1 cut(s) 627
BpiI GAAGAC 2 cut(s) 634, 1737
BpuEI CTTGAG 2 cut(s) 5, 1734
BpuMI CCSGG 1 cut(s) 189
Bsa29I ATCGAT 1 cut(s) 726
BsaI GGTCTC 2 cut(s) 80, 449
BsaJI CCNNGG 2 cut(s) 60, 1557
BsaXI ACNNNNNCTCC 6 cut(s) 854, 884, 1073, 1103, 1557, 1587
Bsc4I CCNNNNNNNGG 1 cut(s) 1222
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 3 cut(s) 633, 1001, 1293
Bse3DI GCAATG 1 cut(s) 1257
BseBI CCWGG 1 cut(s) 828
BseCI ATCGAT 1 cut(s) 726
BseDI CCNNGG 2 cut(s) 60, 1557
BseLI CCNNNNNNNGG 1 cut(s) 1222
BseMI GCAATG 1 cut(s) 1257
BseMII CTCAG 1 cut(s) 1042
BseNI ACTGG 3 cut(s) 633, 1001, 1293
BseRI GAGGAG 1 cut(s) 1094
BseSI GKGCMC 1 cut(s) 1311
BseXI GCAGC 1 cut(s) 104
BseYI CCCAGC 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 103
BshFI GGCC 2 cut(s) 187, 698
BshNI GGYRCC 2 cut(s) 1453, 1483
BshVI ATCGAT 1 cut(s) 726
BsiHKAI GWGCWC 2 cut(s) 1311, 1668
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 2 cut(s) 72, 189
BslFI GGGAC 2 cut(s) 21, 1197
BslI CCNNNNNNNGG 1 cut(s) 1222
BsmAI GTCTC 4 cut(s) 80, 449, 874, 1382
BsmFI GGGAC 2 cut(s) 21, 1197
BsmI GAATGC 1 cut(s) 1749
BsnI GGCC 2 cut(s) 187, 698
Bso31I GGTCTC 2 cut(s) 80, 449
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 2 cut(s) 1311, 1668
Bsp143I GATC 5 cut(s) 262, 940, 1101, 1385, 1645
BspACI CCGC 6 cut(s) 39, 101, 209, 238, 531, 1391
BspANI GGCC 2 cut(s) 187, 698
BspCNI CTCAG 1 cut(s) 1041
BspDI ATCGAT 1 cut(s) 726
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 3 cut(s) 171, 1455, 1485
BspPI GGATC 3 cut(s) 270, 935, 1640
BspQI GCTCTTC 1 cut(s) 102
BspT107I GGYRCC 2 cut(s) 1453, 1483
BspTNI GGTCTC 2 cut(s) 80, 449
BsrDI GCAATG 1 cut(s) 1257
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 3 cut(s) 633, 1001, 1293
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 60, 1557
BssMI GATC 5 cut(s) 262, 940, 1101, 1385, 1645
BssSI CACGAG 2 cut(s) 204, 672
BssT1I CCWWGG 1 cut(s) 60
Bst2BI CACGAG 2 cut(s) 204, 672
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 3 cut(s) 258, 613, 1148
Bst6I CTCTTC 2 cut(s) 102, 560
BstC8I GCNNGC 2 cut(s) 1138, 1711
BstDEI CTNAG 4 cut(s) 275, 851, 1028, 1205
BstFNI CGCG 1 cut(s) 103
BstKTI GATC 5 cut(s) 265, 943, 1104, 1388, 1648
BstMAI GTCTC 4 cut(s) 80, 449, 874, 1382
BstMBI GATC 5 cut(s) 262, 940, 1101, 1385, 1645
BstMWI GCNNNNNNNGC 4 cut(s) 47, 146, 1388, 1715
BstNI CCWGG 1 cut(s) 828
BstSCI CCNGG 2 cut(s) 187, 826
BstSFI CTRYAG 1 cut(s) 972
BstSLI GKGCMC 1 cut(s) 1311
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 104
BstV2I GAAGAC 2 cut(s) 634, 1737
BstX2I RGATCY 1 cut(s) 1645
BstXI CCANNNNNNTGG 1 cut(s) 855
BstYI RGATCY 1 cut(s) 1645
Bsu15I ATCGAT 1 cut(s) 726
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 2 cut(s) 187, 698
BsuTUI ATCGAT 1 cut(s) 726
BtsI GCAGTG 2 cut(s) 1497, 1689
BtsIMutI CAGTG 5 cut(s) 640, 904, 1036, 1497, 1689
Cac8I GCNNGC 2 cut(s) 1138, 1711
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 2 cut(s) 170, 186
ClaI ATCGAT 1 cut(s) 726
CseI GACGC 1 cut(s) 898
CsiI ACCWGGT 1 cut(s) 826
Csp6I GTAC 4 cut(s) 688, 845, 1326, 1754
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 5 cut(s) 266, 917, 1177, 1450, 1811
CviQI GTAC 4 cut(s) 688, 845, 1326, 1754
DdeI CTNAG 4 cut(s) 275, 851, 1028, 1205
DpnI GATC 5 cut(s) 264, 942, 1103, 1387, 1647
DpnII GATC 5 cut(s) 262, 940, 1101, 1385, 1645
DrdI GACNNNNNNGTC 1 cut(s) 596
DseDI GACNNNNNNGTC 1 cut(s) 596
Eam1104I CTCTTC 2 cut(s) 102, 560
EarI CTCTTC 2 cut(s) 102, 560
Eco130I CCWWGG 1 cut(s) 60
Eco31I GGTCTC 2 cut(s) 80, 449
Eco32I GATATC 1 cut(s) 1318
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 2 cut(s) 171, 1740
Eco88I CYCGRG 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 826
EcoRV GATATC 1 cut(s) 1318
EcoT14I CCWWGG 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 5 cut(s) 269, 920, 1180, 1453, 1814
FalI AAGNNNNNCTT 4 cut(s) 834, 866, 1563, 1595
FaqI GGGAC 2 cut(s) 21, 1197
FatI CATG 5 cut(s) 265, 916, 1176, 1449, 1810
FauI CCCGC 1 cut(s) 46
FauNDI CATATG 1 cut(s) 1405
FblI GTMKAC 1 cut(s) 773
Fnu4HI GCNGC 3 cut(s) 6, 93, 239
Fsp4HI GCNGC 3 cut(s) 6, 93, 239
FspBI CTAG 4 cut(s) 341, 1043, 1640, 1706
GluI GCNGC 3 cut(s) 6, 93, 239
GsaI CCCAGC 1 cut(s) 859
HaeIII GGCC 2 cut(s) 187, 698
HapII CCGG 2 cut(s) 72, 189
HgaI GACGC 1 cut(s) 898
Hin1II CATG 5 cut(s) 269, 920, 1180, 1453, 1814
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 9 cut(s) 85, 124, 134, 202, 344, 353, 407, 677, 863
HpaII CCGG 2 cut(s) 72, 189
HphI GGTGA 5 cut(s) 49, 175, 383, 1096, 1282
Hpy166II GTNNAC 3 cut(s) 774, 930, 1309
Hpy188I TCNGA 8 cut(s) 174, 352, 602, 720, 789, 1245, 1422, 1585
Hpy188III TCNNGA 4 cut(s) 77, 79, 411, 674
Hpy8I GTNNAC 3 cut(s) 774, 930, 1309
Hpy99I CGWCG 2 cut(s) 178, 613
HpyAV CCTTC 5 cut(s) 264, 509, 923, 1390, 1601
HpyCH4III ACNGT 3 cut(s) 258, 613, 1148
HpyCH4V TGCA 9 cut(s) 8, 92, 332, 1096, 1309, 1430, 1682, 1787, 1801
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 146, 1388, 1715
HpyF3I CTNAG 4 cut(s) 275, 851, 1028, 1205
Hsp92II CATG 5 cut(s) 269, 920, 1180, 1453, 1814
Kzo9I GATC 5 cut(s) 262, 940, 1101, 1385, 1645
LguI GCTCTTC 1 cut(s) 102
Lsp1109I GCAGC 1 cut(s) 104
LweI GCATC 4 cut(s) 397, 732, 772, 1774
MabI ACCWGGT 1 cut(s) 826
MaeI CTAG 4 cut(s) 341, 1043, 1640, 1706
MaeIII GTNAC 5 cut(s) 55, 199, 837, 1089, 1531
MalI GATC 5 cut(s) 264, 942, 1103, 1387, 1647
MboI GATC 5 cut(s) 262, 940, 1101, 1385, 1645
MfeI CAATTG 3 cut(s) 9, 439, 1778
MflI RGATCY 1 cut(s) 1645
MhlI GDGCHC 2 cut(s) 1311, 1668
MlyI GAGTC 4 cut(s) 143, 196, 416, 857
MmeI TCCRAC 3 cut(s) 197, 240, 1615
MroXI GAANNNNTTC 1 cut(s) 1575
MseI TTAA 5 cut(s) 615, 891, 1193, 1352, 1700
MslI CAYNNNNRTG 2 cut(s) 197, 921
MspA1I CMGCKG 1 cut(s) 1391
MspI CCGG 2 cut(s) 72, 189
MspR9I CCNGG 2 cut(s) 189, 828
MunI CAATTG 3 cut(s) 9, 439, 1778
Mva1269I GAATGC 1 cut(s) 1749
MvaI CCWGG 1 cut(s) 828
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 4 cut(s) 47, 146, 1388, 1715
NciI CCSGG 1 cut(s) 189
NdeI CATATG 1 cut(s) 1405
NdeII GATC 5 cut(s) 262, 940, 1101, 1385, 1645
NlaIII CATG 5 cut(s) 269, 920, 1180, 1453, 1814
NlaIV GGNNCC 3 cut(s) 171, 1455, 1485
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 3 cut(s) 55, 199, 1531
PaeR7I CTCGAG 1 cut(s) 77
PciSI GCTCTTC 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 679
PctI GAATGC 1 cut(s) 1749
PdmI GAANNNNTTC 1 cut(s) 1575
PfeI GAWTC 5 cut(s) 85, 124, 344, 353, 677
PflFI GACNNNGTC 1 cut(s) 866
PflMI CCANNNNNTGG 1 cut(s) 1222
PkrI GCNGC 3 cut(s) 7, 94, 240
PleI GAGTC 4 cut(s) 142, 196, 415, 857
PpsI GAGTC 4 cut(s) 142, 196, 415, 857
Psp6I CCWGG 1 cut(s) 826
PspFI CCCAGC 1 cut(s) 855
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 3 cut(s) 171, 1455, 1485
PspPI GGNCC 2 cut(s) 170, 186
PsuI RGATCY 1 cut(s) 1645
PsyI GACNNNGTC 1 cut(s) 866
RsaI GTAC 4 cut(s) 689, 846, 1327, 1755
RsaNI GTAC 4 cut(s) 688, 845, 1326, 1754
RseI CAYNNNNRTG 2 cut(s) 197, 921
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 5 cut(s) 615, 891, 1193, 1352, 1700
SatI GCNGC 3 cut(s) 6, 93, 239
Sau3AI GATC 5 cut(s) 262, 940, 1101, 1385, 1645
Sau96I GGNCC 2 cut(s) 170, 186
ScaI AGTACT 2 cut(s) 689, 1755
SchI GAGTC 4 cut(s) 143, 196, 416, 857
ScrFI CCNGG 2 cut(s) 189, 828
SduI GDGCHC 2 cut(s) 1311, 1668
SexAI ACCWGGT 1 cut(s) 826
SfaNI GCATC 4 cut(s) 397, 732, 772, 1774
SfcI CTRYAG 1 cut(s) 972
Sfr274I CTCGAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 77
SmiMI CAYNNNNRTG 2 cut(s) 197, 921
SmlI CTYRAG 3 cut(s) 20, 77, 1749
SmoI CTYRAG 3 cut(s) 20, 77, 1749
SpeI ACTAGT 1 cut(s) 1639
SsiI CCGC 6 cut(s) 39, 101, 209, 238, 531, 1391
SspMI CTAG 4 cut(s) 341, 1043, 1640, 1706
StyD4I CCNGG 2 cut(s) 187, 826
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 3 cut(s) 258, 613, 1148
TaqI TCGA 6 cut(s) 78, 356, 384, 585, 608, 726
TatI WGTACW 2 cut(s) 687, 1753
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 5 cut(s) 85, 124, 344, 353, 677
Tru1I TTAA 5 cut(s) 615, 891, 1193, 1352, 1700
Tru9I TTAA 5 cut(s) 615, 891, 1193, 1352, 1700
TscAI CASTG 5 cut(s) 640, 904, 1036, 1497, 1689
TseFI GTSAC 3 cut(s) 55, 199, 1531
TseI GCWGC 2 cut(s) 5, 92
Tsp45I GTSAC 3 cut(s) 55, 199, 1531
TspDTI ATGAA 8 cut(s) 132, 336, 528, 905, 969, 1165, 1179, 1404
TspGWI ACGGA 2 cut(s) 606, 862
TspRI CASTG 5 cut(s) 640, 904, 1036, 1497, 1689
Tth111I GACNNNGTC 1 cut(s) 866
Van91I CCANNNNNTGG 1 cut(s) 1222
VneI GTGCAC 1 cut(s) 1307
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 4 cut(s) 155, 417, 812, 1369
XcmI CCANNNNNNNNNTGG 1 cut(s) 640
XhoI CTCGAG 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 773
XmnI GAANNNNTTC 1 cut(s) 1575
XspI CTAG 4 cut(s) 341, 1043, 1640, 1706
ZrmI AGTACT 2 cut(s) 689, 1755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.