RchiOBHm_Chr2g0102921

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0012563
Physical Location & Seq
Forward (+)
14535881 .. 14537740
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47732

Sequence Viewer

Length: 1860 bp
ATGATGGAGTGCTCTATACTTGTTTTCGTACTTTTCTTTCTATTCACTCCAAAGCTCAAAGCTCAACAAAGCTATGCCGGAAACTCGGTTCTGGACTGTAATAACACTGATGATGCAGGGCCATCCGCGGCTTTTCTTTACACCTGCAATGGTCAGAAGCAGTCCTGCAAGTCTTTCTTGATCTTCAAGTCTCAACCTCCTTACAATTCGCTCTCCGCCATCTCAAACCTCACATCTTCAGACCCATCTGAGCTTGCCCAAATCAACATGATCTCCGACAACACACAGATGTTGCCTCCAGGCAAAGAGGTGATTGTTCCTGTGGTCTGTTCTTGCTCAGGTCAGTACTATGAGGCCAAAACCTCTTTCTCAGTTCCAAGCATCCATGACACTTATTTTACAACAGCAAACAGCACATACCAAGGTTTGTCTACTTGTGATGCACTGATGAGCCACAACAATTACAGTGAATTTAGCGTGGAACCTGGTTTGAAGTTACAGGTACCACTTAGGTGTGCTTGTCCCACAAGAAACCAAACTTTGAATGGTACTAAATTTCTATTGACTTACTTGGTCAGCTGGGATGACTCTGTCTCCGTAGTTAGTGAGAGATTTAATGTCAGTGAAAAGAGCGTCCTTCATGCCAATGGGTTTACAGAAGATGATCCTACTTTGTTTCCCTTTACAACAATTCTTATTCCACTTCCAGTAGAGCCTTCAAGCTCTCAAACCCGAATCCACTACCCACCGCCACTGAAATCTCCTCCGGTTAGTCCTATTCATGGAAAGGCAAGGTCAAGGAAAGTATATGTTTGGATAGCAGGAATTGGAGTTCTTGTACTAGTCCTTTGCTTGATTATCTTTGTGGTCGTTTTCTATCGCCGGAAGAAGGGTAGTCAAAAGAATACTGAAGGGAAATCCAAGAAGAAATTGGATTTGCAGGAAGAATTATATGTCTCCATTGCTAAAGCTGATGCAGGTTTGAAACTCTATAGATATGAAGAATTGGAAAGTGCTACAGAGGATTTCAGCAACAAGAACTGGTTGGGTGGCTCTGTTTATAGTGGTTTTCTCAGTGGAAATGTGCTAGTAGCCATAAAAAAGATGACCAAAGATGTGAGTGAGGAGGTGAAGTTACTGCAAAAGATCAACCACTTCAATCTAATAAGTCTTTTGGGTGCGTGCGAACACTGTAAGGATTTCTACCTTGTTTATGAGTTCATGGAGAATGGTTCATTAAGAGATTGGCTAAGTTGTCCCCAAGTTTTGGGATGGAAGCACAGGGTTCAGATTGCTTTGGACATTGCCAATGGGCTTCACTATCTTCACAACTTCACCAGTCCTGCCTATGTGCACAAGGATATTAAAAGTACCAATGTACTTCTCAATAGAGATTTAAGGGCAAAGATTGCAAATTTTAGTCTCGCAAGATCAGCAGAGAAGGAAACATATGGCTTTTCGTCTCGGAAGTTTGCACAGGTATCAAAAGGCTACATGGCACCAGAATATATAGAATATGGCTTGGTGACACCTGCAATGGATGTATATGCTTTTGGGGTTATTATGCTGGAATTAGTAACAGGAAAAGAAGCTGTTTTTACTGAGGATGGAGTAGAAGTATTTCTTTCAGAAGTTATATTTTCAGTAATGGAAGAAGGAAATGGAGAAGTTGAGCCTGTTGGACTAGTAGATCCCAATTTGAATGTAGAGCACTTGATGGAGTTTGCAGTGAGATTGATAAAGTTAAGTCTAGTCTGCTTGGCTGAAGAACCAGAAGACAGACCCAGAATGCCTCAAGTACTCTCATCTGTGCTGAAAATACAATTGGATGCAAACAGGAAAATGCAGACTTCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

619

Amino Acids

69.06

Weight (kDa)

6.11

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM1_NFP_LYK PF23446 55 - 112 6.3e-18 NFP/LYK LysM1 domain
LysM2_CERK1_LYK3_4_5 PF23472 116 - 171 4.4e-22 LYK3/4/5 LysM2 domain
LysM3_LYK4_5 PF23473 186 - 235 2e-20 LYK4/5 LysM3 domain
PK_Tyr_Ser-Thr PF07714 351 - 602 3.9e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 359 - 602 1.3e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 152, 1540
Acc36I ACCTGC 3 cut(s) 152, 968, 1540
Acc65I GGTACC 1 cut(s) 502
AccB1I GGYRCC 2 cut(s) 502, 1498
AccB7I CCANNNNNTGG 1 cut(s) 1267
AccI GTMKAC 1 cut(s) 431
AccII CGCG 1 cut(s) 128
AciI CCGC 4 cut(s) 126, 128, 216, 749
AclWI GGATC 2 cut(s) 659, 1685
AcsI RAATTY 3 cut(s) 470, 554, 1414
AcuI CTGAAG 3 cut(s) 222, 930, 1785
AfaI GTAC 8 cut(s) 30, 347, 504, 550, 840, 1372, 1380, 1800
AfiI CCNNNNNNNGG 3 cut(s) 782, 889, 1267
AgsI TTSAA 7 cut(s) 187, 493, 544, 720, 985, 1159, 1702
AhlI ACTAGT 2 cut(s) 841, 1684
AjnI CCWGG 2 cut(s) 298, 484
AjuI GAANNNNNNNTTGG 4 cut(s) 350, 382, 1808, 1840
AleI CACNNNNGTG 1 cut(s) 511
AloI GAACNNNNNNTCC 4 cut(s) 72, 104, 816, 848
AluBI AGCT 8 cut(s) 55, 62, 72, 253, 579, 723, 971, 1592
AluI AGCT 8 cut(s) 55, 62, 72, 253, 579, 723, 971, 1592
Alw21I GWGCWC 3 cut(s) 14, 1356, 1713
Alw26I GTCTC 5 cut(s) 195, 598, 961, 1427, 1467
Alw44I GTGCAC 1 cut(s) 1352
AlwI GGATC 2 cut(s) 659, 1685
AoxI GGCC 2 cut(s) 119, 354
ApaLI GTGCAC 1 cut(s) 1352
ApoI RAATTY 3 cut(s) 470, 554, 1414
Asp700I GAANNNNTTC 1 cut(s) 1620
Asp718I GGTACC 1 cut(s) 502
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 4 cut(s) 322, 1141, 1327, 1537
BaeGI GKGCMC 1 cut(s) 1356
BanI GGYRCC 2 cut(s) 502, 1498
BbsI GAAGAC 1 cut(s) 1782
Bbv12I GWGCWC 3 cut(s) 14, 1356, 1713
BccI CCATC 6 cut(s) 130, 227, 253, 1266, 1601, 1711
BciT130I CCWGG 2 cut(s) 300, 486
BcoDI GTCTC 5 cut(s) 195, 598, 961, 1427, 1467
BcuI ACTAGT 2 cut(s) 841, 1684
BfaI CTAG 4 cut(s) 842, 1088, 1685, 1751
BfmI CTRYAG 2 cut(s) 991, 1017
BfuAI ACCTGC 3 cut(s) 152, 968, 1540
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
BmcAI AGTACT 2 cut(s) 347, 1800
Bme1390I CCNGG 2 cut(s) 300, 486
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 3 cut(s) 483, 504, 1500
BmrFI CCNGG 2 cut(s) 300, 486
BmsI GCATC 5 cut(s) 103, 390, 430, 964, 1819
BpiI GAAGAC 1 cut(s) 1782
BpmI CTGGAG 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 1779
BsaJI CCNNGG 2 cut(s) 126, 421
BsaWI WCCGGW 1 cut(s) 766
Bsc4I CCNNNNNNNGG 3 cut(s) 782, 889, 1267
Bse1I ACTGG 3 cut(s) 707, 1046, 1338
Bse3DI GCAATG 4 cut(s) 154, 960, 1302, 1542
BseBI CCWGG 2 cut(s) 300, 486
BseDI CCNNGG 2 cut(s) 126, 421
BseGI GGATG 7 cut(s) 122, 381, 589, 1277, 1546, 1612, 1834
BseLI CCNNNNNNNGG 3 cut(s) 782, 889, 1267
BseMI GCAATG 4 cut(s) 154, 960, 1302, 1542
BseMII CTCAG 5 cut(s) 240, 351, 384, 1087, 1593
BseNI ACTGG 3 cut(s) 707, 1046, 1338
BseRI GAGGAG 2 cut(s) 753, 1139
BseSI GKGCMC 1 cut(s) 1356
BseYI CCCAGC 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 2 cut(s) 121, 356
BshNI GGYRCC 2 cut(s) 502, 1498
BsiHKAI GWGCWC 3 cut(s) 14, 1356, 1713
BsiSI CCGG 3 cut(s) 78, 767, 883
BslFI GGGAC 2 cut(s) 507, 1242
BslI CCNNNNNNNGG 3 cut(s) 782, 889, 1267
BsmAI GTCTC 5 cut(s) 195, 598, 961, 1427, 1467
BsmBI CGTCTC 1 cut(s) 1467
BsmFI GGGAC 2 cut(s) 507, 1242
BsmI GAATGC 1 cut(s) 1794
BsnI GGCC 2 cut(s) 121, 356
Bsp1286I GDGCHC 3 cut(s) 14, 1356, 1713
Bsp143I GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1690
BspACI CCGC 4 cut(s) 126, 128, 216, 749
BspANI GGCC 2 cut(s) 121, 356
BspCNI CTCAG 5 cut(s) 241, 350, 383, 1086, 1594
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 3 cut(s) 483, 504, 1500
BspMI ACCTGC 3 cut(s) 152, 968, 1540
BspPI GGATC 2 cut(s) 659, 1685
BspT107I GGYRCC 2 cut(s) 502, 1498
BsrDI GCAATG 4 cut(s) 154, 960, 1302, 1542
BsrI ACTGG 3 cut(s) 707, 1046, 1338
BssECI CCNNGG 2 cut(s) 126, 421
BssMI GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1690
BssT1I CCWWGG 1 cut(s) 421
Bst2UI CCWGG 2 cut(s) 300, 486
Bst4CI ACNGT 3 cut(s) 98, 467, 1193
BstAPI GCANNNNNTGC 1 cut(s) 1409
BstC8I GCNNGC 2 cut(s) 255, 1183
BstDEI CTNAG 7 cut(s) 249, 337, 370, 509, 1073, 1250, 1602
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 7 cut(s) 122, 381, 589, 1277, 1546, 1612, 1834
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 6 cut(s) 183, 273, 667, 1149, 1433, 1693
BstMAI GTCTC 5 cut(s) 195, 598, 961, 1427, 1467
BstMBI GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1690
BstMWI GCNNNNNNNGC 2 cut(s) 1409, 1433
BstNI CCWGG 2 cut(s) 300, 486
BstSCI CCNGG 2 cut(s) 298, 484
BstSFI CTRYAG 2 cut(s) 991, 1017
BstSLI GKGCMC 1 cut(s) 1356
BstUI CGCG 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 1782
BstX2I RGATCY 1 cut(s) 1690
BstYI RGATCY 1 cut(s) 1690
BsuRI GGCC 2 cut(s) 121, 356
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 7 cut(s) 122, 381, 589, 1277, 1546, 1612, 1834
BtsI GCAGTG 1 cut(s) 1734
BtsIMutI CAGTG 8 cut(s) 105, 443, 472, 628, 752, 1081, 1189, 1734
BveI ACCTGC 3 cut(s) 152, 968, 1540
Cac8I GCNNGC 2 cut(s) 255, 1183
Cfr13I GGNCC 1 cut(s) 119
Cfr42I CCGCGG 1 cut(s) 129
CseI GACGC 1 cut(s) 622
CsiI ACCWGGT 1 cut(s) 484
Csp6I GTAC 8 cut(s) 29, 346, 503, 549, 839, 1371, 1379, 1799
CviAII CATG 6 cut(s) 268, 386, 641, 782, 1222, 1495
CviQI GTAC 8 cut(s) 29, 346, 503, 549, 839, 1371, 1379, 1799
DdeI CTNAG 7 cut(s) 249, 337, 370, 509, 1073, 1250, 1602
DpnI GATC 6 cut(s) 182, 272, 666, 1148, 1432, 1692
DpnII GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1690
EciI GGCGGA 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 421
Eco57I CTGAAG 3 cut(s) 222, 930, 1785
EcoRII CCWGG 2 cut(s) 298, 484
EcoT14I CCWWGG 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 421
Esp3I CGTCTC 1 cut(s) 1467
FaeI CATG 6 cut(s) 271, 389, 644, 785, 1225, 1498
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 1608, 1640
FaqI GGGAC 2 cut(s) 507, 1242
FatI CATG 6 cut(s) 267, 385, 640, 781, 1221, 1494
FauNDI CATATG 1 cut(s) 1450
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 7 cut(s) 109, 368, 596, 1284, 1553, 1619, 1841
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 4 cut(s) 842, 1088, 1685, 1751
GluI GCNGC 1 cut(s) 129
GsaI CCCAGC 1 cut(s) 583
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 2 cut(s) 121, 356
HapII CCGG 3 cut(s) 78, 767, 883
HgaI GACGC 1 cut(s) 622
Hin1II CATG 6 cut(s) 271, 389, 644, 785, 1225, 1498
HinfI GANTC 2 cut(s) 587, 735
HpaII CCGG 3 cut(s) 78, 767, 883
HphI GGTGA 4 cut(s) 322, 1141, 1327, 1537
Hpy166II GTNNAC 3 cut(s) 432, 654, 1354
Hpy188I TCNGA 7 cut(s) 156, 241, 250, 277, 1290, 1467, 1630
Hpy188III TCNNGA 2 cut(s) 92, 178
Hpy8I GTNNAC 3 cut(s) 432, 654, 1354
HpyAV CCTTC 6 cut(s) 647, 726, 883, 905, 1435, 1649
HpyCH4III ACNGT 3 cut(s) 98, 467, 1193
HpyF10VI GCNNNNNNNGC 2 cut(s) 1409, 1433
HpyF3I CTNAG 7 cut(s) 249, 337, 370, 509, 1073, 1250, 1602
Hsp92II CATG 6 cut(s) 271, 389, 644, 785, 1225, 1498
KpnI GGTACC 1 cut(s) 506
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1690
LweI GCATC 5 cut(s) 103, 390, 430, 964, 1819
MabI ACCWGGT 1 cut(s) 484
MaeI CTAG 4 cut(s) 842, 1088, 1685, 1751
MaeIII GTNAC 4 cut(s) 495, 1134, 1525, 1576
MalI GATC 6 cut(s) 182, 272, 666, 1148, 1432, 1692
MboI GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1690
MfeI CAATTG 1 cut(s) 1823
MflI RGATCY 1 cut(s) 1690
MhlI GDGCHC 3 cut(s) 14, 1356, 1713
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 2 cut(s) 300, 1660
MroXI GAANNNNTTC 1 cut(s) 1620
MseI TTAA 5 cut(s) 615, 1238, 1365, 1397, 1745
MslI CAYNNNNRTG 3 cut(s) 287, 511, 645
MspA1I CMGCKG 2 cut(s) 128, 579
MspI CCGG 3 cut(s) 78, 767, 883
MspR9I CCNGG 2 cut(s) 300, 486
MunI CAATTG 1 cut(s) 1823
Mva1269I GAATGC 1 cut(s) 1794
MvaI CCWGG 2 cut(s) 300, 486
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 2 cut(s) 1409, 1433
NdeI CATATG 1 cut(s) 1450
NdeII GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1690
NlaIII CATG 6 cut(s) 271, 389, 644, 785, 1225, 1498
NlaIV GGNNCC 3 cut(s) 483, 504, 1500
NmuCI GTSAC 1 cut(s) 1525
OliI CACNNNNGTG 1 cut(s) 511
PaqCI CACCTGC 2 cut(s) 152, 1540
PctI GAATGC 1 cut(s) 1794
PdmI GAANNNNTTC 1 cut(s) 1620
PfeI GAWTC 1 cut(s) 735
PflFI GACNNNGTC 1 cut(s) 590
PflMI CCANNNNNTGG 1 cut(s) 1267
PkrI GCNGC 1 cut(s) 130
PleI GAGTC 1 cut(s) 581
PpsI GAGTC 1 cut(s) 581
Psp6I CCWGG 2 cut(s) 298, 484
PspFI CCCAGC 1 cut(s) 579
PspGI CCWGG 2 cut(s) 298, 484
PspN4I GGNNCC 3 cut(s) 483, 504, 1500
PspPI GGNCC 1 cut(s) 119
PsuI RGATCY 1 cut(s) 1690
PsyI GACNNNGTC 1 cut(s) 590
PvuII CAGCTG 1 cut(s) 579
RsaI GTAC 8 cut(s) 30, 347, 504, 550, 840, 1372, 1380, 1800
RsaNI GTAC 8 cut(s) 29, 346, 503, 549, 839, 1371, 1379, 1799
RseI CAYNNNNRTG 3 cut(s) 287, 511, 645
SacII CCGCGG 1 cut(s) 129
SaqAI TTAA 5 cut(s) 615, 1238, 1365, 1397, 1745
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1690
Sau96I GGNCC 1 cut(s) 119
ScaI AGTACT 2 cut(s) 347, 1800
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 2 cut(s) 300, 486
SduI GDGCHC 3 cut(s) 14, 1356, 1713
SexAI ACCWGGT 1 cut(s) 484
SfaNI GCATC 5 cut(s) 103, 390, 430, 964, 1819
SfcI CTRYAG 2 cut(s) 991, 1017
Sfr303I CCGCGG 1 cut(s) 129
SgrBI CCGCGG 1 cut(s) 129
SmiMI CAYNNNNRTG 3 cut(s) 287, 511, 645
SmlI CTYRAG 1 cut(s) 1794
SmoI CTYRAG 1 cut(s) 1794
SpeI ACTAGT 2 cut(s) 841, 1684
SsiI CCGC 4 cut(s) 126, 128, 216, 749
SspMI CTAG 4 cut(s) 842, 1088, 1685, 1751
StyD4I CCNGG 2 cut(s) 298, 484
StyI CCWWGG 1 cut(s) 421
TaaI ACNGT 3 cut(s) 98, 467, 1193
TatI WGTACW 4 cut(s) 345, 838, 1378, 1798
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 1 cut(s) 735
Tru1I TTAA 5 cut(s) 615, 1238, 1365, 1397, 1745
Tru9I TTAA 5 cut(s) 615, 1238, 1365, 1397, 1745
TscAI CASTG 8 cut(s) 112, 450, 472, 628, 759, 1081, 1196, 1734
TseFI GTSAC 1 cut(s) 1525
Tsp45I GTSAC 1 cut(s) 1525
TspDTI ATGAA 5 cut(s) 629, 770, 1014, 1210, 1224
TspGWI ACGGA 1 cut(s) 586
TspRI CASTG 8 cut(s) 112, 450, 472, 628, 759, 1081, 1196, 1734
Tth111I GACNNNGTC 1 cut(s) 590
Van91I CCANNNNNTGG 1 cut(s) 1267
VneI GTGCAC 1 cut(s) 1352
XapI RAATTY 3 cut(s) 470, 554, 1414
XcmI CCANNNNNNNNNTGG 2 cut(s) 542, 928
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 1620
XspI CTAG 4 cut(s) 842, 1088, 1685, 1751
ZrmI AGTACT 2 cut(s) 347, 1800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.