Rroxscaffold_2G00140230

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012563
Physical Location & Seq
Reverse (-)
78453983 .. 78455995
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140230.1

Sequence Viewer

Length: 2013 bp
ATGAACTGGTCAACAAAAGCACAAAACTATTATTCAATACATCGACGCGGTTATGCTGATGTTGCAGACATTATCATTGGCAAAGACTATATATTTATCAGTACTTGTTCAAGTTTTAGTAGTCTCAGCAACAAAGAAATTAAGCTGATGATGGAGTGCTCTATACTTGTTTTCGTACTTTTCTTGCTATTCACTCCAAAGCTCAAAGCTCAACAAAGCTATGCCGGAAACTCGGTTCTGGACTGTAATAACACTGATGATGCAGGGCCATCCGCGGCTTTTCTTTACACCTGCAATGGTCAGAAGCATTCCTGCAAGTCTTTCTTGATCTTCAAGTCTCAACCTCCTTACAATTCGCTCTCCGCCATCTCAAACCTCACATCTTCAGACCCATCTGAGCTTGCCCGAATCAACATGATCTCCGACAACACACAGTTGTTGCCTCCAGGCAAAGAGGTGATTGTTCCTGTGGTCTGTTCTTGCTCAGGTCAGTACTATGAGGCCAAAACCTCTTTTTTAGTTCCGAGCATCGATGACACCTATTTTGCAATAGCAAACAGCACATATCAAGGTTTGTCTACTTGTAATGCTCTGATGAGCCACAACAATTACAGTGAATTTAACGTGGAACCTGGTTTGAAGTTACAGGTACCACTTAGGTGTGCTTGTCCCACAAGAAACCAAACTTTCAATGGTACTAAATTTCTGCTGACTTACTTGGTCAGCTGGGATGACTCTGTCTCCGAAGTTAGTGAGAGATTTAATGTCAGTGAAAAGAGCGTCCTACTTGCCAATGGGTTTACAGAAGATGATCCTACTGTGTTTGTCTTTACAACAATTCTTATTCCACTTCCAGTAGAGCCTTCAAGCTCTCAAACCCGAATCCACTACCCACCGCCACTGAAATCTCCTCCGGTTAGTCCTCTTCATGGAAAGGCAAGGTCAAGGAAAGTATATGTTTGGATAGCAGGAATTGGAGTTCTTGTACTAGTCCTTGGCTTGATTATCTTTGTGGTCCTTTTCTATCGCCGGAAAAAGGGTTGTCAAAAGAATACTGAAGGGAAATCCAAAAAGAAATTGGATTTGCAAGAAGAATTATATGTCTCAATTGCTAAAGCTGATGCAGGTTTGAAACTCTATAGATATGAAGAATTGGAAAGTGCTACAGAGGATTTCAGCAACAAGAACTGGTTGGGTGGCTCTGTTTATAGTGGTTTTCTCAGTGGAAATGTGCTAGTAGCCATAAAAAAGATGACCAAAGATGTGAGTGAGGAGGTGAAGTTACTGCAAAAGATCAACCACTTCAATCTAATAAGTCTTTTGGGTGCGTGCGAACACCGTAAGGATTTCTACCTTGTTTATGAGTTCATGGAGAATGGTTCATTAAGAGATTGGCTAAGTTGTCCCCAAGTTTTGGGATGGAAGCACAGGGTTCAGATTGCTTTGGACATTGCCAATGGGCTTCACTATCTTCACAACTTCACCAGTCCTGCCTATGTGCACAAGGATATCAAAAGTACCAATGTTCTTCTCAATAGAGATTTAAGGGCAAAGATTGGAAATTTTAGTCTCGCAAGATCAGCGGAGAAGGAAACATATGGCTTTTCATCTCGGAAGTTTGCACAGGTATCAAAAGGCTACATGGCACCAGAATATATAGAATATGGCTTGGTGACACCAGCAGTGGATGTATATGCTTTTGGGGTTGTTATGCTGGAATTAGTCACAGGAAAAGAAGCTGTTTTTACCGAGGATGGAGTTGAAGTATTTCTTTCAGAAGCTATATTTTCAGTAATGGAAGGAGGAAATGAAGAAGTTGAGCCTGTTGGACTAGTAGATCCCAATTTGAATGTAGAGCACTTGATGGAGTTTGCAGTGAGATTGATAAAGTTAAGTCTAGCCTGCTTGGCTGAAGAACCAGAAGACAGACCCAGAATGCCTCAAGTACTCTCATCTGTGCTGAAAATACAATTGGATGCAAACAGGAAAATGCAGAACTTCCATGTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

670

Amino Acids

74.96

Weight (kDa)

6.69

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM1_NFP_LYK PF23446 104 - 161 1.8e-17 NFP/LYK LysM1 domain
LysM2_CERK1_LYK3_4_5 PF23472 165 - 220 1.8e-23 LYK3/4/5 LysM2 domain
LysM3_LYK4_5 PF23473 235 - 284 2.2e-19 LYK4/5 LysM3 domain
PK_Tyr_Ser-Thr PF07714 400 - 651 2.4e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 409 - 651 4.6e-37 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 299
Acc36I ACCTGC 2 cut(s) 299, 1115
Acc65I GGTACC 1 cut(s) 649
AccB1I GGYRCC 2 cut(s) 649, 1645
AccB7I CCANNNNNTGG 1 cut(s) 1414
AccI GTMKAC 1 cut(s) 578
AccII CGCG 2 cut(s) 48, 275
AciI CCGC 6 cut(s) 48, 273, 275, 363, 896, 1583
AclWI GGATC 2 cut(s) 806, 1832
AcsI RAATTY 3 cut(s) 617, 701, 1561
AcuI CTGAAG 3 cut(s) 369, 1077, 1932
AfaI GTAC 8 cut(s) 103, 177, 494, 651, 697, 987, 1519, 1947
AfiI CCNNNNNNNGG 3 cut(s) 929, 1036, 1414
AhlI ACTAGT 2 cut(s) 988, 1831
AjnI CCWGG 2 cut(s) 445, 631
AjuI GAANNNNNNNTTGG 2 cut(s) 1955, 1987
AleI CACNNNNGTG 1 cut(s) 658
AloI GAACNNNNNNTCC 4 cut(s) 219, 251, 963, 995
Alw21I GWGCWC 3 cut(s) 161, 1503, 1860
Alw26I GTCTC 5 cut(s) 128, 342, 745, 1108, 1574
Alw44I GTGCAC 1 cut(s) 1499
AlwI GGATC 2 cut(s) 806, 1832
AoxI GGCC 2 cut(s) 266, 501
ApaLI GTGCAC 1 cut(s) 1499
ApoI RAATTY 3 cut(s) 617, 701, 1561
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 1 cut(s) 1767
Asp718I GGTACC 1 cut(s) 649
AspS9I GGNCC 2 cut(s) 266, 1015
AsuHPI GGTGA 4 cut(s) 469, 1288, 1474, 1684
AvaII GGWCC 1 cut(s) 1015
BaeGI GKGCMC 1 cut(s) 1503
BanI GGYRCC 2 cut(s) 649, 1645
BbsI GAAGAC 1 cut(s) 1929
Bbv12I GWGCWC 3 cut(s) 161, 1503, 1860
BccI CCATC 7 cut(s) 145, 277, 374, 400, 1413, 1748, 1858
BciT130I CCWGG 2 cut(s) 447, 633
BcoDI GTCTC 5 cut(s) 128, 342, 745, 1108, 1574
BcuI ACTAGT 2 cut(s) 988, 1831
BfaI CTAG 4 cut(s) 989, 1235, 1832, 1898
BfmI CTRYAG 2 cut(s) 1138, 1164
BfuAI ACCTGC 2 cut(s) 299, 1115
BglI GCCNNNNNGGC 1 cut(s) 1907
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
BmcAI AGTACT 3 cut(s) 103, 494, 1947
Bme1390I CCNGG 2 cut(s) 447, 633
Bme18I GGWCC 1 cut(s) 1015
BmgT120I GGNCC 2 cut(s) 266, 1015
BmiI GGNNCC 3 cut(s) 630, 651, 1647
BmrFI CCNGG 2 cut(s) 447, 633
BmsI GCATC 4 cut(s) 250, 537, 1111, 1966
BpiI GAAGAC 1 cut(s) 1929
BpmI CTGGAG 1 cut(s) 429
Bpu10I CCTNAGC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 1926
Bsa29I ATCGAT 1 cut(s) 531
BsaJI CCNNGG 3 cut(s) 273, 994, 1749
BsaWI WCCGGW 1 cut(s) 913
Bsc4I CCNNNNNNNGG 3 cut(s) 929, 1036, 1414
Bse1I ACTGG 4 cut(s) 11, 854, 1193, 1485
Bse3DI GCAATG 2 cut(s) 301, 1449
BseBI CCWGG 2 cut(s) 447, 633
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 3 cut(s) 273, 994, 1749
BseGI GGATG 6 cut(s) 269, 736, 1424, 1693, 1759, 1981
BseLI CCNNNNNNNGG 3 cut(s) 929, 1036, 1414
BseMI GCAATG 2 cut(s) 301, 1449
BseMII CTCAG 4 cut(s) 139, 387, 498, 1234
BseNI ACTGG 4 cut(s) 11, 854, 1193, 1485
BseRI GAGGAG 2 cut(s) 900, 1286
BseSI GKGCMC 1 cut(s) 1503
BseYI CCCAGC 1 cut(s) 726
Bsh1236I CGCG 2 cut(s) 48, 275
BshFI GGCC 2 cut(s) 268, 503
BshNI GGYRCC 2 cut(s) 649, 1645
BshVI ATCGAT 1 cut(s) 531
BsiHKAI GWGCWC 3 cut(s) 161, 1503, 1860
BsiSI CCGG 3 cut(s) 225, 914, 1030
BslFI GGGAC 2 cut(s) 654, 1389
BslI CCNNNNNNNGG 3 cut(s) 929, 1036, 1414
BsmAI GTCTC 5 cut(s) 128, 342, 745, 1108, 1574
BsmFI GGGAC 2 cut(s) 654, 1389
BsmI GAATGC 2 cut(s) 307, 1941
BsnI GGCC 2 cut(s) 268, 503
Bsp1286I GDGCHC 3 cut(s) 161, 1503, 1860
Bsp143I GATC 6 cut(s) 327, 417, 811, 1293, 1577, 1837
BspACI CCGC 6 cut(s) 48, 273, 275, 363, 896, 1583
BspANI GGCC 2 cut(s) 268, 503
BspCNI CTCAG 4 cut(s) 138, 388, 497, 1233
BspDI ATCGAT 1 cut(s) 531
BspFNI CGCG 2 cut(s) 48, 275
BspLI GGNNCC 3 cut(s) 630, 651, 1647
BspMI ACCTGC 2 cut(s) 299, 1115
BspPI GGATC 2 cut(s) 806, 1832
BspT107I GGYRCC 2 cut(s) 649, 1645
BsrDI GCAATG 2 cut(s) 301, 1449
BsrI ACTGG 4 cut(s) 11, 854, 1193, 1485
BssECI CCNNGG 3 cut(s) 273, 994, 1749
BssMI GATC 6 cut(s) 327, 417, 811, 1293, 1577, 1837
BssT1I CCWWGG 1 cut(s) 994
Bst2UI CCWGG 2 cut(s) 447, 633
Bst4CI ACNGT 5 cut(s) 245, 435, 614, 820, 1340
Bst6I CTCTTC 1 cut(s) 930
BstC8I GCNNGC 3 cut(s) 402, 1330, 1903
BstDEI CTNAG 6 cut(s) 125, 396, 484, 656, 1220, 1397
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 6 cut(s) 269, 736, 1424, 1693, 1759, 1981
BstFNI CGCG 2 cut(s) 48, 275
BstKTI GATC 6 cut(s) 330, 420, 814, 1296, 1580, 1840
BstMAI GTCTC 5 cut(s) 128, 342, 745, 1108, 1574
BstMBI GATC 6 cut(s) 327, 417, 811, 1293, 1577, 1837
BstMWI GCNNNNNNNGC 3 cut(s) 62, 1580, 1907
BstNI CCWGG 2 cut(s) 447, 633
BstSCI CCNGG 2 cut(s) 445, 631
BstSFI CTRYAG 2 cut(s) 1138, 1164
BstSLI GKGCMC 1 cut(s) 1503
BstUI CGCG 2 cut(s) 48, 275
BstV2I GAAGAC 1 cut(s) 1929
BstX2I RGATCY 1 cut(s) 1837
BstYI RGATCY 1 cut(s) 1837
Bsu15I ATCGAT 1 cut(s) 531
BsuRI GGCC 2 cut(s) 268, 503
BsuTUI ATCGAT 1 cut(s) 531
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 6 cut(s) 269, 736, 1424, 1693, 1759, 1981
BtsI GCAGTG 2 cut(s) 1689, 1881
BtsIMutI CAGTG 7 cut(s) 252, 619, 775, 899, 1228, 1689, 1881
BveI ACCTGC 2 cut(s) 299, 1115
Cac8I GCNNGC 3 cut(s) 402, 1330, 1903
Cfr13I GGNCC 2 cut(s) 266, 1015
Cfr42I CCGCGG 1 cut(s) 276
ClaI ATCGAT 1 cut(s) 531
CseI GACGC 2 cut(s) 54, 769
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 8 cut(s) 102, 176, 493, 650, 696, 986, 1518, 1946
CviAII CATG 5 cut(s) 415, 929, 1369, 1642, 2003
CviQI GTAC 8 cut(s) 102, 176, 493, 650, 696, 986, 1518, 1946
DdeI CTNAG 6 cut(s) 125, 396, 484, 656, 1220, 1397
DpnI GATC 6 cut(s) 329, 419, 813, 1295, 1579, 1839
DpnII GATC 6 cut(s) 327, 417, 811, 1293, 1577, 1837
Eam1104I CTCTTC 1 cut(s) 930
EarI CTCTTC 1 cut(s) 930
EciI GGCGGA 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 994
Eco32I GATATC 1 cut(s) 1510
Eco47I GGWCC 1 cut(s) 1015
Eco57I CTGAAG 3 cut(s) 369, 1077, 1932
EcoRII CCWGG 2 cut(s) 445, 631
EcoRV GATATC 1 cut(s) 1510
EcoT14I CCWWGG 1 cut(s) 994
ErhI CCWWGG 1 cut(s) 994
FaeI CATG 5 cut(s) 418, 932, 1372, 1645, 2006
FalI AAGNNNNNCTT 4 cut(s) 308, 340, 1755, 1787
FaqI GGGAC 2 cut(s) 654, 1389
FatI CATG 5 cut(s) 414, 928, 1368, 1641, 2002
FauNDI CATATG 1 cut(s) 1597
FblI GTMKAC 1 cut(s) 578
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 6 cut(s) 256, 743, 1431, 1700, 1766, 1988
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 4 cut(s) 989, 1235, 1832, 1898
GluI GCNGC 1 cut(s) 276
GsaI CCCAGC 1 cut(s) 730
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 2 cut(s) 268, 503
HapII CCGG 3 cut(s) 225, 914, 1030
HgaI GACGC 2 cut(s) 54, 769
Hin1II CATG 5 cut(s) 418, 932, 1372, 1645, 2006
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 3 cut(s) 408, 734, 882
HpaII CCGG 3 cut(s) 225, 914, 1030
HphI GGTGA 4 cut(s) 469, 1288, 1474, 1684
Hpy166II GTNNAC 4 cut(s) 12, 579, 801, 1501
Hpy188III TCNNGA 2 cut(s) 239, 325
Hpy8I GTNNAC 4 cut(s) 12, 579, 801, 1501
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 4 cut(s) 873, 1052, 1582, 1793
HpyCH4III ACNGT 5 cut(s) 245, 435, 614, 820, 1340
HpyCH4IV ACGT 1 cut(s) 624
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 1580, 1907
HpyF3I CTNAG 6 cut(s) 125, 396, 484, 656, 1220, 1397
HpySE526I ACGT 1 cut(s) 624
Hsp92II CATG 5 cut(s) 418, 932, 1372, 1645, 2006
KpnI GGTACC 1 cut(s) 653
KspI CCGCGG 1 cut(s) 276
Kzo9I GATC 6 cut(s) 327, 417, 811, 1293, 1577, 1837
LweI GCATC 4 cut(s) 250, 537, 1111, 1966
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 4 cut(s) 989, 1235, 1832, 1898
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 4 cut(s) 642, 1281, 1672, 1723
MalI GATC 6 cut(s) 329, 419, 813, 1295, 1579, 1839
MboI GATC 6 cut(s) 327, 417, 811, 1293, 1577, 1837
MfeI CAATTG 2 cut(s) 1107, 1970
MflI RGATCY 1 cut(s) 1837
MhlI GDGCHC 3 cut(s) 161, 1503, 1860
MlyI GAGTC 1 cut(s) 728
MmeI TCCRAC 2 cut(s) 447, 1807
MroXI GAANNNNTTC 1 cut(s) 1767
MseI TTAA 6 cut(s) 141, 621, 762, 1385, 1544, 1892
MslI CAYNNNNRTG 1 cut(s) 658
MspA1I CMGCKG 3 cut(s) 275, 726, 1583
MspI CCGG 3 cut(s) 225, 914, 1030
MspR9I CCNGG 2 cut(s) 447, 633
MunI CAATTG 2 cut(s) 1107, 1970
Mva1269I GAATGC 2 cut(s) 307, 1941
MvaI CCWGG 2 cut(s) 447, 633
MvnI CGCG 2 cut(s) 48, 275
MwoI GCNNNNNNNGC 3 cut(s) 62, 1580, 1907
NdeI CATATG 1 cut(s) 1597
NdeII GATC 6 cut(s) 327, 417, 811, 1293, 1577, 1837
NlaIII CATG 5 cut(s) 418, 932, 1372, 1645, 2006
NlaIV GGNNCC 3 cut(s) 630, 651, 1647
NmuCI GTSAC 2 cut(s) 1672, 1723
OliI CACNNNNGTG 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 299
PctI GAATGC 2 cut(s) 307, 1941
PdmI GAANNNNTTC 1 cut(s) 1767
PfeI GAWTC 2 cut(s) 408, 882
PflFI GACNNNGTC 1 cut(s) 737
PflMI CCANNNNNTGG 1 cut(s) 1414
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 1 cut(s) 728
PpsI GAGTC 1 cut(s) 728
Psp6I CCWGG 2 cut(s) 445, 631
PspFI CCCAGC 1 cut(s) 726
PspGI CCWGG 2 cut(s) 445, 631
PspN4I GGNNCC 3 cut(s) 630, 651, 1647
PspPI GGNCC 2 cut(s) 266, 1015
PsuI RGATCY 1 cut(s) 1837
PsyI GACNNNGTC 1 cut(s) 737
PvuII CAGCTG 1 cut(s) 726
RsaI GTAC 8 cut(s) 103, 177, 494, 651, 697, 987, 1519, 1947
RsaNI GTAC 8 cut(s) 102, 176, 493, 650, 696, 986, 1518, 1946
RseI CAYNNNNRTG 1 cut(s) 658
SacII CCGCGG 1 cut(s) 276
SaqAI TTAA 6 cut(s) 141, 621, 762, 1385, 1544, 1892
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 6 cut(s) 327, 417, 811, 1293, 1577, 1837
Sau96I GGNCC 2 cut(s) 266, 1015
ScaI AGTACT 3 cut(s) 103, 494, 1947
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 2 cut(s) 447, 633
SduI GDGCHC 3 cut(s) 161, 1503, 1860
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 4 cut(s) 250, 537, 1111, 1966
SfcI CTRYAG 2 cut(s) 1138, 1164
Sfr303I CCGCGG 1 cut(s) 276
SgrBI CCGCGG 1 cut(s) 276
SinI GGWCC 1 cut(s) 1015
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 1 cut(s) 1941
SmoI CTYRAG 1 cut(s) 1941
SpeI ACTAGT 2 cut(s) 988, 1831
SsiI CCGC 6 cut(s) 48, 273, 275, 363, 896, 1583
SspMI CTAG 4 cut(s) 989, 1235, 1832, 1898
StyD4I CCNGG 2 cut(s) 445, 631
StyI CCWWGG 1 cut(s) 994
TaaI ACNGT 5 cut(s) 245, 435, 614, 820, 1340
TaiI ACGT 1 cut(s) 627
TaqI TCGA 2 cut(s) 43, 531
TatI WGTACW 4 cut(s) 101, 492, 985, 1945
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 2 cut(s) 408, 882
Tru1I TTAA 6 cut(s) 141, 621, 762, 1385, 1544, 1892
Tru9I TTAA 6 cut(s) 141, 621, 762, 1385, 1544, 1892
TscAI CASTG 7 cut(s) 259, 619, 775, 906, 1228, 1689, 1881
TseFI GTSAC 2 cut(s) 1672, 1723
Tsp45I GTSAC 2 cut(s) 1672, 1723
TspDTI ATGAA 7 cut(s) 17, 917, 1161, 1357, 1371, 1596, 1824
TspRI CASTG 7 cut(s) 259, 619, 775, 906, 1228, 1689, 1881
Tth111I GACNNNGTC 1 cut(s) 737
Van91I CCANNNNNTGG 1 cut(s) 1414
VneI GTGCAC 1 cut(s) 1499
VpaK11BI GGWCC 1 cut(s) 1015
XapI RAATTY 3 cut(s) 617, 701, 1561
XcmI CCANNNNNNNNNTGG 2 cut(s) 689, 1075
XmiI GTMKAC 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 1767
XspI CTAG 4 cut(s) 989, 1235, 1832, 1898
ZrmI AGTACT 3 cut(s) 103, 494, 1947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.