Rw2G012400

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012563
Physical Location & Seq
Forward (+)
12914165 .. 12916022
1858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012400.1

Sequence Viewer

Length: 1806 bp
ATGATGGAGTGCTCTATACTTGTTTTCGTACTTTTCTTGCTATTCACTCCAAAGCTCAAAGCTCAACAAAGCTATGCCGGAAACTCTGTTCTGGACTGTAATAACACTGATGATGCAGGGCCATCCGCGGCTTTTCTTTACACCTGCAATGGTCAGAAGCAGTCCTGCAAGTCTTTCTTGATCTTCAAGTCTCAACCTCCTTACAATTCGCTCTCCGCCATCTCAAACCTCACATCTTCAGACCCATCTGAGCTTGCCCGAATCAACATGATCTCCGACAACACACAGTTGTTGCCTCCAGGCAAAGAGGTGATTGTTCCTGTGGTCTGTTCTTGCTCAGGTCAGTACTATGAGGCCAAAACCTCTTTTAGTTTGTCTACTTGTGATGCACTGATGAGCCAGAACAATTACAGTGAATTTAGCGTGGAACCTGGTTTGAAGTTACAGGTACCACTTAGGTGTGCTTGTCCCACAAGAAACCAAACTTTGAATAGTACTAAATTTCTATTGACTTACTTGGTCAGCTGGGATGACACTGTCTCCGTAGTTAGTGAGAGATTTAATGTCAGTGAAAAGAGCGTCCTTCATGCCAATGGGTTTACAGAAGATGATCCTACTTTGTTTCCCTTTACAACAATTCTTATTCCACTTCCAGTAGAGCCTTCAAGCTCTCAAACCCGAATCCACTACCCACCGCCACTGAAATCTCCTCCGGTTAGTCCTATTCATGGAAAGGCAAGGTCAAGGAAAGTATATGTTTGGATAGCACGAATTGGAGTTCTTGTACTAGTCCTTTGCTTGATTATCTTTGTGGTGGTTTTCTATCCCCGGAAAAAGGGTTGTCAAAAGAATACTGAAGGGAAATCCAAAAAGAAATTGGATTTGCAGGAAGAATTATATGTCTCCATTGCTAAAGCTGATGCAGGTTTGAAACTCTATAGATATGAAGAATTGGAAAATGCTACAGAGGATTTCAGCAACAGGAACTGGTTGGGTGGCTCTGTTTATAGTGGCTTTCTCAGTGGAAATGTGCTAGTAGCCATAAAAAAGATGACCAAAGATGTGAGTGAGGAGGTGAAGTTACTGCAAAAGATCAACCACTTCAATCTAATAAGTCTTTTGGGTGCGTGCGAACACTGTAAGGATTTCTACCTTGTTTATGAGTTCATGGAGAATGGTTCATTAAGAGATTGGCTAAGTTGTCCCCAAGTTTTGGGATGGAAGCACAGGGTTCAGATTGCTTTGGACATTGCCAATGGGCTTCACTATCTTCACAACTTCACCAGTCCTGCCTATGTGCACAAGGATATTAAAAGTACCAATGTACTTCTCAATAGAGATTTAAGGGCAAAGATTGCAAATTTTAGTCTCGCAAGATCAGCGGAGAAGGAAACATATGGCTTTTCGTCTCGGAAGTTTGCACAGGTATCAAAAGGCTACATGGCACCAGAATATATAGAATATGGCTTGGTGACACCTGCAATGGATGTATATGCTTTTGGGGTTATTATGCTGGAATTAGTAACAGGAAAAGAAGCTGTTTTTACTGAGGATGGAGTTGAAGTATTTCTTTCAGAAGTTATATTTTCAGTAATGGAAGAAGGAAATGGAGAAGTTGAGCCTGTTGGACTAGTAGATCCCAATTTGAATGTAGAGCACTTGATGGAGTTTGCAGTGAGATTGATAAAGTTAAGTCTAGTCTGCTTGGCTGAAGAACCAGAAGACAGACCCAGAATGCCTCAAGTACTCTCATCTGTGCTGAAAATACAATTGGATGCAAACAGGAAAATGCAGACTTCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

601

Amino Acids

67.2

Weight (kDa)

6.26

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM1_NFP_LYK PF23446 55 - 112 1.5e-17 NFP/LYK LysM1 domain
LysM2_CERK1_LYK3_4_5 PF23472 125 - 153 1.3e-08 LYK3/4/5 LysM2 domain
LysM3_LYK4_5 PF23473 168 - 217 2.4e-20 LYK4/5 LysM3 domain
PK_Tyr_Ser-Thr PF07714 333 - 584 3.6e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 341 - 584 1.2e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 152, 1486
Acc36I ACCTGC 3 cut(s) 152, 914, 1486
Acc65I GGTACC 1 cut(s) 448
AccB1I GGYRCC 2 cut(s) 448, 1444
AccB7I CCANNNNNTGG 1 cut(s) 1213
AccI GTMKAC 1 cut(s) 377
AccII CGCG 1 cut(s) 128
AciI CCGC 5 cut(s) 126, 128, 216, 695, 1382
AclWI GGATC 2 cut(s) 605, 1631
AcsI RAATTY 3 cut(s) 416, 500, 1360
AcuI CTGAAG 3 cut(s) 222, 876, 1731
AfaI GTAC 8 cut(s) 30, 347, 450, 496, 786, 1318, 1326, 1746
AfiI CCNNNNNNNGG 3 cut(s) 728, 835, 1213
AgsI TTSAA 8 cut(s) 187, 439, 490, 666, 931, 1105, 1562, 1648
AhlI ACTAGT 2 cut(s) 787, 1630
AjnI CCWGG 2 cut(s) 298, 430
AjuI GAANNNNNNNTTGG 2 cut(s) 1754, 1786
AleI CACNNNNGTG 1 cut(s) 457
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
AluBI AGCT 8 cut(s) 55, 62, 72, 253, 525, 669, 917, 1538
AluI AGCT 8 cut(s) 55, 62, 72, 253, 525, 669, 917, 1538
Alw21I GWGCWC 3 cut(s) 14, 1302, 1659
Alw26I GTCTC 5 cut(s) 195, 544, 907, 1373, 1413
Alw44I GTGCAC 1 cut(s) 1298
AlwI GGATC 2 cut(s) 605, 1631
AlwNI CAGNNNCTG 1 cut(s) 987
AoxI GGCC 2 cut(s) 119, 354
ApaLI GTGCAC 1 cut(s) 1298
ApoI RAATTY 3 cut(s) 416, 500, 1360
Asp700I GAANNNNTTC 1 cut(s) 1566
Asp718I GGTACC 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 829
AsuHPI GGTGA 4 cut(s) 322, 1087, 1273, 1483
BaeGI GKGCMC 1 cut(s) 1302
BanI GGYRCC 2 cut(s) 448, 1444
BbsI GAAGAC 1 cut(s) 1728
Bbv12I GWGCWC 3 cut(s) 14, 1302, 1659
BccI CCATC 6 cut(s) 130, 227, 253, 1212, 1547, 1657
BciT130I CCWGG 2 cut(s) 300, 432
BcnI CCSGG 1 cut(s) 829
BcoDI GTCTC 5 cut(s) 195, 544, 907, 1373, 1413
BcuI ACTAGT 2 cut(s) 787, 1630
BfaI CTAG 4 cut(s) 788, 1034, 1631, 1697
BfmI CTRYAG 2 cut(s) 937, 963
BfuAI ACCTGC 3 cut(s) 152, 914, 1486
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
BmcAI AGTACT 3 cut(s) 347, 496, 1746
Bme1390I CCNGG 3 cut(s) 300, 432, 829
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 3 cut(s) 429, 450, 1446
BmrFI CCNGG 3 cut(s) 300, 432, 829
BmsI GCATC 4 cut(s) 103, 376, 910, 1765
BpiI GAAGAC 1 cut(s) 1728
BpmI CTGGAG 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 1725
BpuMI CCSGG 1 cut(s) 829
BsaJI CCNNGG 2 cut(s) 126, 827
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 3 cut(s) 728, 835, 1213
Bse1I ACTGG 3 cut(s) 653, 992, 1284
Bse3DI GCAATG 4 cut(s) 154, 906, 1248, 1488
BseBI CCWGG 2 cut(s) 300, 432
BseDI CCNNGG 2 cut(s) 126, 827
BseGI GGATG 6 cut(s) 122, 535, 1223, 1492, 1558, 1780
BseLI CCNNNNNNNGG 3 cut(s) 728, 835, 1213
BseMI GCAATG 4 cut(s) 154, 906, 1248, 1488
BseMII CTCAG 4 cut(s) 240, 351, 1033, 1539
BseNI ACTGG 3 cut(s) 653, 992, 1284
BseRI GAGGAG 2 cut(s) 699, 1085
BseSI GKGCMC 1 cut(s) 1302
BseYI CCCAGC 1 cut(s) 525
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 2 cut(s) 121, 356
BshNI GGYRCC 2 cut(s) 448, 1444
BsiHKAI GWGCWC 3 cut(s) 14, 1302, 1659
BsiSI CCGG 3 cut(s) 78, 713, 829
BslFI GGGAC 2 cut(s) 453, 1188
BslI CCNNNNNNNGG 3 cut(s) 728, 835, 1213
BsmAI GTCTC 5 cut(s) 195, 544, 907, 1373, 1413
BsmBI CGTCTC 1 cut(s) 1413
BsmFI GGGAC 2 cut(s) 453, 1188
BsmI GAATGC 1 cut(s) 1740
BsnI GGCC 2 cut(s) 121, 356
Bsp1286I GDGCHC 3 cut(s) 14, 1302, 1659
Bsp143I GATC 6 cut(s) 180, 270, 610, 1092, 1376, 1636
BspACI CCGC 5 cut(s) 126, 128, 216, 695, 1382
BspANI GGCC 2 cut(s) 121, 356
BspCNI CTCAG 4 cut(s) 241, 350, 1032, 1540
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 3 cut(s) 429, 450, 1446
BspMI ACCTGC 3 cut(s) 152, 914, 1486
BspPI GGATC 2 cut(s) 605, 1631
BspT107I GGYRCC 2 cut(s) 448, 1444
BsrDI GCAATG 4 cut(s) 154, 906, 1248, 1488
BsrI ACTGG 3 cut(s) 653, 992, 1284
BssECI CCNNGG 2 cut(s) 126, 827
BssMI GATC 6 cut(s) 180, 270, 610, 1092, 1376, 1636
Bst2UI CCWGG 2 cut(s) 300, 432
Bst4CI ACNGT 5 cut(s) 98, 288, 413, 538, 1139
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstC8I GCNNGC 2 cut(s) 255, 1129
BstDEI CTNAG 6 cut(s) 249, 337, 455, 1019, 1196, 1548
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 6 cut(s) 122, 535, 1223, 1492, 1558, 1780
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 6 cut(s) 183, 273, 613, 1095, 1379, 1639
BstMAI GTCTC 5 cut(s) 195, 544, 907, 1373, 1413
BstMBI GATC 6 cut(s) 180, 270, 610, 1092, 1376, 1636
BstMWI GCNNNNNNNGC 2 cut(s) 1355, 1379
BstNI CCWGG 2 cut(s) 300, 432
BstSCI CCNGG 3 cut(s) 298, 430, 827
BstSFI CTRYAG 2 cut(s) 937, 963
BstSLI GKGCMC 1 cut(s) 1302
BstUI CGCG 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 1728
BstX2I RGATCY 1 cut(s) 1636
BstYI RGATCY 1 cut(s) 1636
BsuRI GGCC 2 cut(s) 121, 356
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 6 cut(s) 122, 535, 1223, 1492, 1558, 1780
BtsI GCAGTG 1 cut(s) 1680
BtsIMutI CAGTG 9 cut(s) 105, 389, 418, 534, 574, 698, 1027, 1135, 1680
BveI ACCTGC 3 cut(s) 152, 914, 1486
Cac8I GCNNGC 2 cut(s) 255, 1129
CaiI CAGNNNCTG 1 cut(s) 987
Cfr13I GGNCC 1 cut(s) 119
Cfr42I CCGCGG 1 cut(s) 129
CseI GACGC 1 cut(s) 568
CsiI ACCWGGT 1 cut(s) 430
Csp6I GTAC 8 cut(s) 29, 346, 449, 495, 785, 1317, 1325, 1745
CviAII CATG 5 cut(s) 268, 587, 728, 1168, 1441
CviQI GTAC 8 cut(s) 29, 346, 449, 495, 785, 1317, 1325, 1745
DdeI CTNAG 6 cut(s) 249, 337, 455, 1019, 1196, 1548
DpnI GATC 6 cut(s) 182, 272, 612, 1094, 1378, 1638
DpnII GATC 6 cut(s) 180, 270, 610, 1092, 1376, 1636
EciI GGCGGA 1 cut(s) 205
Eco57I CTGAAG 3 cut(s) 222, 876, 1731
EcoRII CCWGG 2 cut(s) 298, 430
Esp3I CGTCTC 1 cut(s) 1413
FaeI CATG 5 cut(s) 271, 590, 731, 1171, 1444
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 1554, 1586
FaqI GGGAC 2 cut(s) 453, 1188
FatI CATG 5 cut(s) 267, 586, 727, 1167, 1440
FauNDI CATATG 1 cut(s) 1396
FblI GTMKAC 1 cut(s) 377
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 6 cut(s) 109, 542, 1230, 1499, 1565, 1787
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 4 cut(s) 788, 1034, 1631, 1697
GluI GCNGC 1 cut(s) 129
GsaI CCCAGC 1 cut(s) 529
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 2 cut(s) 121, 356
HapII CCGG 3 cut(s) 78, 713, 829
HgaI GACGC 1 cut(s) 568
Hin1II CATG 5 cut(s) 271, 590, 731, 1171, 1444
HinfI GANTC 2 cut(s) 261, 681
HpaII CCGG 3 cut(s) 78, 713, 829
HphI GGTGA 4 cut(s) 322, 1087, 1273, 1483
Hpy166II GTNNAC 3 cut(s) 378, 600, 1300
Hpy188I TCNGA 7 cut(s) 156, 241, 250, 277, 1236, 1413, 1576
Hpy188III TCNNGA 2 cut(s) 92, 178
Hpy8I GTNNAC 3 cut(s) 378, 600, 1300
HpyAV CCTTC 5 cut(s) 593, 672, 851, 1381, 1595
HpyCH4III ACNGT 5 cut(s) 98, 288, 413, 538, 1139
HpyF10VI GCNNNNNNNGC 2 cut(s) 1355, 1379
HpyF3I CTNAG 6 cut(s) 249, 337, 455, 1019, 1196, 1548
Hsp92II CATG 5 cut(s) 271, 590, 731, 1171, 1444
KpnI GGTACC 1 cut(s) 452
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 6 cut(s) 180, 270, 610, 1092, 1376, 1636
LweI GCATC 4 cut(s) 103, 376, 910, 1765
MabI ACCWGGT 1 cut(s) 430
MaeI CTAG 4 cut(s) 788, 1034, 1631, 1697
MaeIII GTNAC 4 cut(s) 441, 1080, 1471, 1522
MalI GATC 6 cut(s) 182, 272, 612, 1094, 1378, 1638
MboI GATC 6 cut(s) 180, 270, 610, 1092, 1376, 1636
MboII GAAGA 9 cut(s) 175, 228, 617, 902, 959, 1262, 1610, 1724, 1733
MfeI CAATTG 1 cut(s) 1769
MflI RGATCY 1 cut(s) 1636
MhlI GDGCHC 3 cut(s) 14, 1302, 1659
MmeI TCCRAC 2 cut(s) 300, 1606
MroXI GAANNNNTTC 1 cut(s) 1566
MseI TTAA 5 cut(s) 561, 1184, 1311, 1343, 1691
MslI CAYNNNNRTG 2 cut(s) 457, 591
MspA1I CMGCKG 3 cut(s) 128, 525, 1382
MspI CCGG 3 cut(s) 78, 713, 829
MspR9I CCNGG 3 cut(s) 300, 432, 829
MunI CAATTG 1 cut(s) 1769
Mva1269I GAATGC 1 cut(s) 1740
MvaI CCWGG 2 cut(s) 300, 432
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 2 cut(s) 1355, 1379
NciI CCSGG 1 cut(s) 829
NdeI CATATG 1 cut(s) 1396
NdeII GATC 6 cut(s) 180, 270, 610, 1092, 1376, 1636
NlaIII CATG 5 cut(s) 271, 590, 731, 1171, 1444
NlaIV GGNNCC 3 cut(s) 429, 450, 1446
NmuCI GTSAC 1 cut(s) 1471
OliI CACNNNNGTG 1 cut(s) 457
PaqCI CACCTGC 2 cut(s) 152, 1486
PctI GAATGC 1 cut(s) 1740
PdmI GAANNNNTTC 1 cut(s) 1566
PfeI GAWTC 2 cut(s) 261, 681
PflFI GACNNNGTC 1 cut(s) 536
PflMI CCANNNNNTGG 1 cut(s) 1213
PkrI GCNGC 1 cut(s) 130
Psp6I CCWGG 2 cut(s) 298, 430
PspFI CCCAGC 1 cut(s) 525
PspGI CCWGG 2 cut(s) 298, 430
PspN4I GGNNCC 3 cut(s) 429, 450, 1446
PspPI GGNCC 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 987
PsuI RGATCY 1 cut(s) 1636
PsyI GACNNNGTC 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 8 cut(s) 30, 347, 450, 496, 786, 1318, 1326, 1746
RsaNI GTAC 8 cut(s) 29, 346, 449, 495, 785, 1317, 1325, 1745
RseI CAYNNNNRTG 2 cut(s) 457, 591
SacII CCGCGG 1 cut(s) 129
SaqAI TTAA 5 cut(s) 561, 1184, 1311, 1343, 1691
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 6 cut(s) 180, 270, 610, 1092, 1376, 1636
Sau96I GGNCC 1 cut(s) 119
ScaI AGTACT 3 cut(s) 347, 496, 1746
ScrFI CCNGG 3 cut(s) 300, 432, 829
SduI GDGCHC 3 cut(s) 14, 1302, 1659
SexAI ACCWGGT 1 cut(s) 430
SfaNI GCATC 4 cut(s) 103, 376, 910, 1765
SfcI CTRYAG 2 cut(s) 937, 963
Sfr303I CCGCGG 1 cut(s) 129
SgrBI CCGCGG 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 457, 591
SmlI CTYRAG 1 cut(s) 1740
SmoI CTYRAG 1 cut(s) 1740
SpeI ACTAGT 2 cut(s) 787, 1630
SsiI CCGC 5 cut(s) 126, 128, 216, 695, 1382
SspMI CTAG 4 cut(s) 788, 1034, 1631, 1697
StyD4I CCNGG 3 cut(s) 298, 430, 827
TaaI ACNGT 5 cut(s) 98, 288, 413, 538, 1139
TatI WGTACW 5 cut(s) 345, 494, 784, 1324, 1744
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 2 cut(s) 261, 681
Tru1I TTAA 5 cut(s) 561, 1184, 1311, 1343, 1691
Tru9I TTAA 5 cut(s) 561, 1184, 1311, 1343, 1691
TscAI CASTG 9 cut(s) 112, 396, 418, 541, 574, 705, 1027, 1142, 1680
TseFI GTSAC 1 cut(s) 1471
Tsp45I GTSAC 1 cut(s) 1471
TspDTI ATGAA 5 cut(s) 575, 716, 960, 1156, 1170
TspGWI ACGGA 1 cut(s) 532
TspRI CASTG 9 cut(s) 112, 396, 418, 541, 574, 705, 1027, 1142, 1680
Tth111I GACNNNGTC 1 cut(s) 536
Van91I CCANNNNNTGG 1 cut(s) 1213
VneI GTGCAC 1 cut(s) 1298
XapI RAATTY 3 cut(s) 416, 500, 1360
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XmiI GTMKAC 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 1566
XspI CTAG 4 cut(s) 788, 1034, 1631, 1697
ZrmI AGTACT 3 cut(s) 347, 496, 1746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.