MD15G1270700.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012563
Physical Location & Seq
Forward (+)
23499259 .. 23499648
390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1270700.v1.1.491

Sequence Viewer

Length: 390 bp
ATGCAAGAGAACAATTACAGTGCATTAAACTTGTTGTCCGGTATGAAGTTAGAGGTACCGCTGAGATGTGCTTGTCCTGCAAGCAACCAAACTGCCGATGGTACTGAATTTCTAATGACTTACTTGGTCAGCTTGGATGATTCGGTCCCTAAAGTCAGTAAGAGATTTAATGCAAGTGTAAAGAGTGTAGCTTATGCAAATGGGTTTTCAGAGGATGATACTCCTTTGTTAGCATTTACACCGATTCTAATTCCACTGTTGGTAGAGCCTTGGAGCTCTCAACCCAGGCACCGCCATTCCTTTCTCCTCTGCTTACTCATATTCATGGAAATAGAAGATCTAAGAATGTATGTGTTTGGGCTGAAGTTGGAATTGAAATTCTTCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

130

Amino Acids

14.61

Weight (kDa)

5.29

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM3_LYK4_5 PF23473 37 - 86 4.5e-16 LYK4/5 LysM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 55
AccB1I GGYRCC 2 cut(s) 55, 288
AciI CCGC 2 cut(s) 59, 292
AcsI RAATTY 2 cut(s) 107, 377
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 2 cut(s) 57, 103
AgsI TTSAA 1 cut(s) 376
AjnI CCWGG 1 cut(s) 284
AluBI AGCT 3 cut(s) 132, 191, 276
AluI AGCT 3 cut(s) 132, 191, 276
Alw21I GWGCWC 1 cut(s) 278
ApoI RAATTY 2 cut(s) 107, 377
Asp700I GAANNNNTTC 1 cut(s) 380
Asp718I GGTACC 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 145
AvaII GGWCC 1 cut(s) 145
BanI GGYRCC 2 cut(s) 55, 288
BanII GRGCYC 1 cut(s) 278
Bbv12I GWGCWC 1 cut(s) 278
BccI CCATC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 286
BfaI CTAG 1 cut(s) 388
BglII AGATCT 1 cut(s) 337
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 3 cut(s) 57, 147, 290
BmrFI CCNGG 1 cut(s) 286
BsaJI CCNNGG 2 cut(s) 269, 284
BsaWI WCCGGW 1 cut(s) 38
BseBI CCWGG 1 cut(s) 286
BseDI CCNNGG 2 cut(s) 269, 284
BseGI GGATG 2 cut(s) 142, 220
BseMII CTCAG 1 cut(s) 53
BseRI GAGGAG 1 cut(s) 296
BshNI GGYRCC 2 cut(s) 55, 288
BsiHKAI GWGCWC 1 cut(s) 278
BsiSI CCGG 1 cut(s) 39
BslFI GGGAC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 278
Bsp143I GATC 1 cut(s) 337
BspACI CCGC 2 cut(s) 59, 292
BspCNI CTCAG 1 cut(s) 54
BspLI GGNNCC 3 cut(s) 57, 147, 290
BspT107I GGYRCC 2 cut(s) 55, 288
BssECI CCNNGG 2 cut(s) 269, 284
BssMI GATC 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 2 cut(s) 20, 258
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 2 cut(s) 62, 341
BstF5I GGATG 2 cut(s) 142, 220
BstKTI GATC 1 cut(s) 340
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 286
BstSCI CCNGG 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BtsCI GGATG 2 cut(s) 142, 220
BtsIMutI CAGTG 2 cut(s) 25, 254
Cac8I GCNNGC 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 145
Csp6I GTAC 2 cut(s) 56, 102
CviAII CATG 1 cut(s) 325
CviJI RGCY 5 cut(s) 132, 191, 268, 276, 361
CviKI_1 RGCY 5 cut(s) 132, 191, 268, 276, 361
CviQI GTAC 2 cut(s) 56, 102
DdeI CTNAG 2 cut(s) 62, 341
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
Ecl136II GAGCTC 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 278
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 276
Eco57I CTGAAG 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 284
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 278
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 1 cut(s) 328
FaiI YATR 5 cut(s) 44, 195, 320, 326, 351
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 324
FokI GGATG 2 cut(s) 149, 227
FriOI GRGCYC 1 cut(s) 278
FspBI CTAG 1 cut(s) 388
HapII CCGG 1 cut(s) 39
Hin1II CATG 1 cut(s) 328
HinfI GANTC 2 cut(s) 140, 244
HpaII CCGG 1 cut(s) 39
Hpy188I TCNGA 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 20, 258
HpyCH4V TGCA 5 cut(s) 4, 23, 80, 173, 197
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 2 cut(s) 62, 341
Hsp92II CATG 1 cut(s) 328
KpnI GGTACC 1 cut(s) 59
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 4 cut(s) 52, 90, 271, 298
MaeI CTAG 1 cut(s) 388
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 2 cut(s) 347, 373
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 278
MluCI AATT 5 cut(s) 13, 107, 249, 371, 377
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 3 cut(s) 46, 205, 317
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 2 cut(s) 26, 168
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 1 cut(s) 286
MvaI CCWGG 1 cut(s) 286
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeII GATC 1 cut(s) 337
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 3 cut(s) 57, 147, 290
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 2 cut(s) 140, 244
Psp124BI GAGCTC 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 3 cut(s) 57, 147, 290
PspPI GGNCC 1 cut(s) 145
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 2 cut(s) 57, 103
RsaNI GTAC 2 cut(s) 56, 102
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 26, 168
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 1 cut(s) 278
SetI ASST 4 cut(s) 57, 134, 193, 278
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 5 cut(s) 13, 107, 249, 371, 377
SsiI CCGC 2 cut(s) 59, 292
SspMI CTAG 1 cut(s) 388
SstI GAGCTC 1 cut(s) 278
StyD4I CCNGG 1 cut(s) 284
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 2 cut(s) 20, 258
TaqII GACCGA 1 cut(s) 133
TasI AATT 5 cut(s) 13, 107, 249, 371, 377
TfiI GAWTC 2 cut(s) 140, 244
Tru1I TTAA 2 cut(s) 26, 168
Tru9I TTAA 2 cut(s) 26, 168
TscAI CASTG 2 cut(s) 25, 261
TspDTI ATGAA 2 cut(s) 59, 313
TspRI CASTG 2 cut(s) 25, 261
VpaK11BI GGWCC 1 cut(s) 145
XapI RAATTY 2 cut(s) 107, 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.