MD02G1156300.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012563
Physical Location & Seq
Forward (+)
13013475 .. 13014107
633 bp
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UTR
Exon/CDS
Intron
MD02G1156300.v1.1.491

Sequence Viewer

Length: 633 bp
ATGAAATTAAAGGTGCCACTGAGATGTGCTTGTCCCACAAGCAACCAAACTGCCGATGGTACTAAATTTCTAATGACTTACTTGGTCAGCTTGGATGATTCTTTCCCTGACTTGAGTAAGAGATTTAATGTAAGTGTAAAGAGTGTAGCTTATGCAAATAGGTTTACAGAAGCTGATCCTCCTTTGCTAGCATTTACACCCACTCTAATTCCACTGTCGGTGGAGCCTTCGAGCTCTCAAACCCTAATTCACCACCTACCTGGGCCACCGCCATTCCTTTCTCCTCCAGTTACTTCTATTCATGGAAATAGAAGATCTAAGGATGTACTTATTTGGGTTGGAATTGGAATTGGAATTCTTCTGATAGTCCTCTGCTTGGTTATCTCTGTGGTCTTCTTTCATTGCCAGAAAAAGGGTTCTCAAAAGAATACAGAAGGAAAGAAGAAAAGGGCAATGGAGGAAGATTTATTTGTTACCATTTCTCAAGTTGATGTAGGGTTGAAAGTCTTCAAATACGGAGAATTGAAACATGCTACGGAGGATTTCAGCGAAAAGAACTGGTTGGGGGGCTCTGTACACAAGGGTTTAGGGAAAAGCTCTAGCCATAAAGAAGATGAGCAAAGATGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

211

Amino Acids

23.19

Weight (kDa)

8.93

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM3_LYK4_5 PF23473 23 - 72 7.5e-13 LYK4/5 LysM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 13
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 170
AcsI RAATTY 2 cut(s) 65, 354
AfaI GTAC 3 cut(s) 61, 327, 576
AfiI CCNNNNNNNGG 2 cut(s) 376, 412
AgsI TTSAA 3 cut(s) 502, 511, 526
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 5 cut(s) 90, 149, 173, 234, 597
AluI AGCT 5 cut(s) 90, 149, 173, 234, 597
Alw21I GWGCWC 1 cut(s) 236
AlwI GGATC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 173
AoxI GGCC 1 cut(s) 263
ApoI RAATTY 2 cut(s) 65, 354
Asp700I GAANNNNTTC 1 cut(s) 506
AspS9I GGNCC 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 242
AsuNHI GCTAGC 1 cut(s) 187
BanI GGYRCC 1 cut(s) 13
BanII GRGCYC 2 cut(s) 236, 572
BbsI GAAGAC 2 cut(s) 385, 499
Bbv12I GWGCWC 1 cut(s) 236
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 261
BfaI CTAG 2 cut(s) 188, 600
BglII AGATCT 1 cut(s) 314
Bme1390I CCNGG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 2 cut(s) 15, 225
BmrFI CCNGG 1 cut(s) 261
BmtI GCTAGC 1 cut(s) 191
BpiI GAAGAC 2 cut(s) 385, 499
BpmI CTGGAG 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 133, 468
BsaJI CCNNGG 1 cut(s) 260
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 412
Bse1I ACTGG 2 cut(s) 287, 563
Bse3DI GCAATG 2 cut(s) 400, 459
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 260
BseGI GGATG 2 cut(s) 100, 328
BseLI CCNNNNNNNGG 2 cut(s) 376, 412
BseMI GCAATG 2 cut(s) 400, 459
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 2 cut(s) 287, 563
BseRI GAGGAG 1 cut(s) 273
BshFI GGCC 1 cut(s) 265
BshNI GGYRCC 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 236
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 376, 412
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 265
Bsp1286I GDGCHC 2 cut(s) 236, 572
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 2 cut(s) 175, 314
BspACI CCGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 265
BspCNI CTCAG 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 15, 225
BspOI GCTAGC 1 cut(s) 191
BspPI GGATC 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 13
BsrDI GCAATG 2 cut(s) 400, 459
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 2 cut(s) 287, 563
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 2 cut(s) 175, 314
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 574
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 2 cut(s) 20, 318
BstF5I GGATG 2 cut(s) 100, 328
BstKTI GATC 2 cut(s) 178, 317
BstMBI GATC 2 cut(s) 175, 314
BstNI CCWGG 1 cut(s) 261
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 259
BstV2I GAAGAC 2 cut(s) 385, 499
BstX2I RGATCY 1 cut(s) 314
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 314
BsuRI GGCC 1 cut(s) 265
BtsCI GGATG 2 cut(s) 100, 328
BtsIMutI CAGTG 2 cut(s) 17, 212
Cac8I GCNNGC 1 cut(s) 189
CaiI CAGNNNCTG 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 3 cut(s) 60, 326, 575
CviAII CATG 2 cut(s) 302, 530
CviJI RGCY 9 cut(s) 90, 149, 173, 226, 234, 265, 570, 597, 603
CviKI_1 RGCY 9 cut(s) 90, 149, 173, 226, 234, 265, 570, 597, 603
CviQI GTAC 3 cut(s) 60, 326, 575
DdeI CTNAG 2 cut(s) 20, 318
DpnI GATC 2 cut(s) 177, 316
DpnII GATC 2 cut(s) 175, 314
Ecl136II GAGCTC 1 cut(s) 234
Eco24I GRGCYC 2 cut(s) 236, 572
Eco53kI GAGCTC 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 234
EcoRI GAATTC 1 cut(s) 354
EcoRII CCWGG 1 cut(s) 259
EcoT38I GRGCYC 2 cut(s) 236, 572
FaeI CATG 2 cut(s) 305, 533
FaiI YATR 4 cut(s) 153, 303, 531, 606
FaqI GGGAC 1 cut(s) 18
FatI CATG 2 cut(s) 301, 529
FokI GGATG 2 cut(s) 107, 335
FriOI GRGCYC 2 cut(s) 236, 572
FspBI CTAG 2 cut(s) 188, 600
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 1 cut(s) 265
Hin1II CATG 2 cut(s) 305, 533
HinfI GANTC 1 cut(s) 98
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 165, 577
Hpy188I TCNGA 1 cut(s) 363
Hpy8I GTNNAC 2 cut(s) 165, 577
HpyAV CCTTC 2 cut(s) 237, 428
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4V TGCA 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 20, 318
Hsp92II CATG 2 cut(s) 305, 533
Kzo9I GATC 2 cut(s) 175, 314
LmnI GCTCC 1 cut(s) 223
LpnPI CCDG 6 cut(s) 120, 246, 273, 300, 419, 544
MaeI CTAG 2 cut(s) 188, 600
MaeIII GTNAC 3 cut(s) 289, 472, 626
MalI GATC 2 cut(s) 177, 316
MboI GATC 2 cut(s) 175, 314
MboII GAAGA 7 cut(s) 324, 350, 385, 454, 473, 499, 623
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 236, 572
MluCI AATT 8 cut(s) 5, 65, 207, 246, 342, 348, 354, 521
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 5 cut(s) 189, 294, 380, 451, 532
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 2 cut(s) 8, 126
MslI CAYNNNNRTG 1 cut(s) 22
MspR9I CCNGG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 261
NdeII GATC 2 cut(s) 175, 314
NheI GCTAGC 1 cut(s) 187
NlaIII CATG 2 cut(s) 305, 533
NlaIV GGNNCC 2 cut(s) 15, 225
NmuCI GTSAC 1 cut(s) 626
NspI RCATGY 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 15, 225
PspPI GGNCC 1 cut(s) 263
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 314
RsaI GTAC 3 cut(s) 61, 327, 576
RsaNI GTAC 3 cut(s) 60, 326, 575
RseI CAYNNNNRTG 1 cut(s) 22
SacI GAGCTC 1 cut(s) 236
SaqAI TTAA 2 cut(s) 8, 126
Sau3AI GATC 2 cut(s) 175, 314
Sau96I GGNCC 1 cut(s) 263
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 2 cut(s) 236, 572
SetI ASST 9 cut(s) 15, 92, 151, 164, 175, 236, 258, 262, 599
SmiMI CAYNNNNRTG 1 cut(s) 22
SmlI CTYRAG 2 cut(s) 112, 483
SmoI CTYRAG 2 cut(s) 112, 483
Sse9I AATT 8 cut(s) 5, 65, 207, 246, 342, 348, 354, 521
SsiI CCGC 1 cut(s) 269
SspMI CTAG 2 cut(s) 188, 600
SstI GAGCTC 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 259
TaaI ACNGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 230
TasI AATT 8 cut(s) 5, 65, 207, 246, 342, 348, 354, 521
TatI WGTACW 2 cut(s) 325, 574
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 2 cut(s) 8, 126
Tru9I TTAA 2 cut(s) 8, 126
TscAI CASTG 2 cut(s) 24, 219
TseFI GTSAC 1 cut(s) 626
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 3 cut(s) 17, 290, 389
TspGWI ACGGA 2 cut(s) 531, 551
TspRI CASTG 2 cut(s) 24, 219
XapI RAATTY 2 cut(s) 65, 354
XceI RCATGY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 2 cut(s) 188, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.