Rh2BG165100

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012563
Physical Location & Seq
Forward (+)
14543593 .. 14545480
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG165100.1

Sequence Viewer

Length: 1866 bp
ATGATGGAGTGCTCTATACTTGTTTTCGTACTTTTCTTGCTATTCACTCCAAAGCTCAAAGCTCAACAAAGCTATGCCGGAAACTCGGTTCTGGACTGTAATAACACTGATGATGCAGGGCCATCCGCGGCTTTTCTTTACACCTGCAATGGTCAGAAGCAGTCCTGCAAGTCTTTCTTGATCTTCAAGTCTCAACCTCCTTACAATTCACTCTCCGCCATCTCAAACCTCACATCTTCAGACCCATCTGAGCTTGCCCGAATCAACATGATCTCCGACAACACACAGTTGTTGCCTCCAGGCAAAGAGGTGATTGTTCCTGTGGTCTGTTCTTGCTCAGGTCAGTACTATGAGGCCAAAACCTCTATTTTAGTTCCGAGCATCGATGACACCTATTTTACAATAGCAAACAGCACATATCAAGGTTTGTCTACTTGTGATGCTCTGATGAGCCACAACAATTACAGTGAATTTAGCGTGAAACCTGGTTTGAAGTTACAAGTAGCACTTAGGTGTGCTTGTCCCACAAGAAACCAAACTTTGAATGGTACTAAATCTCTGTTGACTTACTTGGTCAGCTGGGATGACTCTGTCTCCGTAGTTAGTGAGAGATTTAATGTCAGTGAAAAGAGCGTCCTTCATGCCAATGGGTTTACAGAAGATGATCCTACTTTGTTTCCCTTTACAACAATTCTTATTCCACTTCCAGTAGAGCCTTCAAGCTCTCACACCCAAATCCACTACCCACCACCACTGAAATCTCCTCCGGTTAGTCCTATTCATGGAAAGGCAAGGTCAAGGAAAGTATATGTTTGGATAGCAGGAATTGGAGTTCTTGTACTAGTCCTTTGCATGATTATCTTTGTGGTCGTTTTCTATCGCCAGAAAAAGGGTTGTCAAAAGAATACTGAAGGGAAATCCAAAAAGAAATTGGATTTGCAGGAAGAATTATATGTCTCCATTGCTAAAGCTGATGCAGGTTTGAAACTCTATAGATATGAAGAATTGGAAAGTGCTACAGAGGATTTCAGCAACAAGAACTGGTTGGGTGGCTCTGTTTATAGTGGTTTTCTCAGTGGAAATGTTCTAGTAGCCATAAAAAAGATGACCAAAGATGTGAGTGAGGAGGTGAAGTTACTGCAAAAGATCAACCACTTCAATCTAATAAGTCTTTTGGGTGCGTGCGAACACTGTAAGGATTTCTACCTTGTTTATGAGTTCATGGAGAATGGTTCATTAAGAGATTGGCTAAGTTGTCCCCAAGTTTTGGGATGGAAGCACAGGGTTCAGATTGCTTTGGACATTGCCAATGGGCTTCACTATCTTCACAACTTCACCAGTCCTGCCTATGTGCACAAGGATATTAAAAGTACCAATGTACTTCTCAATAGAGATTTAAGGGCAAAGATTGCAAATTTTAGTCTCGCAAGATCAGCGGAGAAGGAAACATATGGCTTTTCGTCTCGGAAGTTTGCACAGGTATCAAAAGGCTACATGGCACCAGAATATATAGAATATGGCTTGGTGACACCTGCAATGGATGTATATGCTTTTGGGGTTATTATGCTGGAATTAGTAACAGGAAAAGAAGCTGTTTTTACTGAGGATGGAGTTGAAGTATTTCTTTCAGAAGTTATATTTTCAGTAATGGAAGAAGGAAATGGAGAAGTTGAGCCTGTTCCTGTTGGACTAGTAGATCCCAATTTGAATGTAGAGCACTTGATGGAGTTTGCAGTGAGATTGATAAAGTTAAGTCTAGTCTGCTTGGCTGAAGAACCAGAAGACAGACCCAGAATGCCTCAAGTACTCTCATCTGTGCTGAAAATACAATTGGATGCAAACAGGAAAATGCAGACTTCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

621

Amino Acids

69.12

Weight (kDa)

6.11

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM1_NFP_LYK PF23446 55 - 112 1.6e-17 NFP/LYK LysM1 domain
LysM2_CERK1_LYK3_4_5 PF23472 116 - 171 1.6e-21 LYK3/4/5 LysM2 domain
LysM3_LYK4_5 PF23473 187 - 235 1.3e-19 LYK4/5 LysM3 domain
PK_Tyr_Ser-Thr PF07714 351 - 603 1.8e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 359 - 604 2.2e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 152, 1540
Acc36I ACCTGC 3 cut(s) 152, 968, 1540
AccB1I GGYRCC 1 cut(s) 1498
AccB7I CCANNNNNTGG 1 cut(s) 1267
AccI GTMKAC 1 cut(s) 431
AccII CGCG 1 cut(s) 128
AciI CCGC 4 cut(s) 126, 128, 216, 1436
AclWI GGATC 2 cut(s) 659, 1691
AcsI RAATTY 2 cut(s) 470, 1414
AcuI CTGAAG 3 cut(s) 222, 930, 1791
AfaI GTAC 7 cut(s) 30, 347, 550, 840, 1372, 1380, 1806
AfiI CCNNNNNNNGG 3 cut(s) 782, 889, 1267
AgsI TTSAA 8 cut(s) 187, 493, 544, 720, 985, 1159, 1616, 1708
AhlI ACTAGT 2 cut(s) 841, 1690
AjnI CCWGG 2 cut(s) 298, 484
AjuI GAANNNNNNNTTGG 2 cut(s) 1814, 1846
AleI CACNNNNGTG 1 cut(s) 511
AloI GAACNNNNNNTCC 4 cut(s) 72, 104, 816, 848
AluBI AGCT 8 cut(s) 55, 62, 72, 253, 579, 723, 971, 1592
AluI AGCT 8 cut(s) 55, 62, 72, 253, 579, 723, 971, 1592
Alw21I GWGCWC 3 cut(s) 14, 1356, 1719
Alw26I GTCTC 5 cut(s) 195, 598, 961, 1427, 1467
Alw44I GTGCAC 1 cut(s) 1352
AlwI GGATC 2 cut(s) 659, 1691
AoxI GGCC 2 cut(s) 119, 354
ApaLI GTGCAC 1 cut(s) 1352
ApoI RAATTY 2 cut(s) 470, 1414
Asp700I GAANNNNTTC 1 cut(s) 1620
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 4 cut(s) 322, 1141, 1327, 1537
BaeGI GKGCMC 1 cut(s) 1356
BanI GGYRCC 1 cut(s) 1498
BbsI GAAGAC 1 cut(s) 1788
Bbv12I GWGCWC 3 cut(s) 14, 1356, 1719
BccI CCATC 6 cut(s) 130, 227, 253, 1266, 1601, 1717
BciT130I CCWGG 2 cut(s) 300, 486
BcoDI GTCTC 5 cut(s) 195, 598, 961, 1427, 1467
BcuI ACTAGT 2 cut(s) 841, 1690
BfaI CTAG 4 cut(s) 842, 1088, 1691, 1757
BfmI CTRYAG 2 cut(s) 991, 1017
BfuAI ACCTGC 3 cut(s) 152, 968, 1540
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
BmcAI AGTACT 2 cut(s) 347, 1806
Bme1390I CCNGG 2 cut(s) 300, 486
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 1500
BmrFI CCNGG 2 cut(s) 300, 486
BmsI GCATC 5 cut(s) 103, 390, 430, 964, 1825
BpiI GAAGAC 1 cut(s) 1788
BpmI CTGGAG 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 1785
Bsa29I ATCGAT 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 766
Bsc4I CCNNNNNNNGG 3 cut(s) 782, 889, 1267
Bse1I ACTGG 3 cut(s) 707, 1046, 1338
Bse3DI GCAATG 4 cut(s) 154, 960, 1302, 1542
BseBI CCWGG 2 cut(s) 300, 486
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 6 cut(s) 122, 589, 1277, 1546, 1612, 1840
BseLI CCNNNNNNNGG 3 cut(s) 782, 889, 1267
BseMI GCAATG 4 cut(s) 154, 960, 1302, 1542
BseMII CTCAG 4 cut(s) 240, 351, 1087, 1593
BseNI ACTGG 3 cut(s) 707, 1046, 1338
BseRI GAGGAG 2 cut(s) 753, 1139
BseSI GKGCMC 1 cut(s) 1356
BseYI CCCAGC 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 2 cut(s) 121, 356
BshNI GGYRCC 1 cut(s) 1498
BshVI ATCGAT 1 cut(s) 384
BsiHKAI GWGCWC 3 cut(s) 14, 1356, 1719
BsiSI CCGG 2 cut(s) 78, 767
BslFI GGGAC 2 cut(s) 507, 1242
BslI CCNNNNNNNGG 3 cut(s) 782, 889, 1267
BsmAI GTCTC 5 cut(s) 195, 598, 961, 1427, 1467
BsmBI CGTCTC 1 cut(s) 1467
BsmFI GGGAC 2 cut(s) 507, 1242
BsmI GAATGC 1 cut(s) 1800
BsnI GGCC 2 cut(s) 121, 356
Bsp1286I GDGCHC 3 cut(s) 14, 1356, 1719
Bsp143I GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1696
BspACI CCGC 4 cut(s) 126, 128, 216, 1436
BspANI GGCC 2 cut(s) 121, 356
BspCNI CTCAG 4 cut(s) 241, 350, 1086, 1594
BspDI ATCGAT 1 cut(s) 384
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 1 cut(s) 1500
BspMI ACCTGC 3 cut(s) 152, 968, 1540
BspPI GGATC 2 cut(s) 659, 1691
BspT107I GGYRCC 1 cut(s) 1498
BsrDI GCAATG 4 cut(s) 154, 960, 1302, 1542
BsrI ACTGG 3 cut(s) 707, 1046, 1338
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1696
Bst2UI CCWGG 2 cut(s) 300, 486
Bst4CI ACNGT 4 cut(s) 98, 288, 467, 1193
BstAPI GCANNNNNTGC 1 cut(s) 1409
BstC8I GCNNGC 2 cut(s) 255, 1183
BstDEI CTNAG 6 cut(s) 249, 337, 509, 1073, 1250, 1602
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 6 cut(s) 122, 589, 1277, 1546, 1612, 1840
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 6 cut(s) 183, 273, 667, 1149, 1433, 1699
BstMAI GTCTC 5 cut(s) 195, 598, 961, 1427, 1467
BstMBI GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1696
BstMWI GCNNNNNNNGC 2 cut(s) 1409, 1433
BstNI CCWGG 2 cut(s) 300, 486
BstSCI CCNGG 2 cut(s) 298, 484
BstSFI CTRYAG 2 cut(s) 991, 1017
BstSLI GKGCMC 1 cut(s) 1356
BstUI CGCG 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 1788
BstX2I RGATCY 1 cut(s) 1696
BstYI RGATCY 1 cut(s) 1696
Bsu15I ATCGAT 1 cut(s) 384
BsuRI GGCC 2 cut(s) 121, 356
BsuTUI ATCGAT 1 cut(s) 384
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 6 cut(s) 122, 589, 1277, 1546, 1612, 1840
BtsI GCAGTG 1 cut(s) 1740
BtsIMutI CAGTG 7 cut(s) 105, 472, 628, 752, 1081, 1189, 1740
BveI ACCTGC 3 cut(s) 152, 968, 1540
Cac8I GCNNGC 2 cut(s) 255, 1183
Cfr13I GGNCC 1 cut(s) 119
Cfr42I CCGCGG 1 cut(s) 129
ClaI ATCGAT 1 cut(s) 384
CseI GACGC 1 cut(s) 622
CsiI ACCWGGT 1 cut(s) 484
Csp6I GTAC 7 cut(s) 29, 346, 549, 839, 1371, 1379, 1805
CviAII CATG 6 cut(s) 268, 641, 782, 853, 1222, 1495
CviQI GTAC 7 cut(s) 29, 346, 549, 839, 1371, 1379, 1805
DdeI CTNAG 6 cut(s) 249, 337, 509, 1073, 1250, 1602
DpnI GATC 6 cut(s) 182, 272, 666, 1148, 1432, 1698
DpnII GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1696
EciI GGCGGA 1 cut(s) 205
Eco57I CTGAAG 3 cut(s) 222, 930, 1791
EcoRII CCWGG 2 cut(s) 298, 484
Esp3I CGTCTC 1 cut(s) 1467
FaeI CATG 6 cut(s) 271, 644, 785, 856, 1225, 1498
FalI AAGNNNNNCTT 6 cut(s) 161, 193, 492, 524, 1608, 1640
FaqI GGGAC 2 cut(s) 507, 1242
FatI CATG 6 cut(s) 267, 640, 781, 852, 1221, 1494
FauNDI CATATG 1 cut(s) 1450
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 6 cut(s) 109, 596, 1284, 1553, 1619, 1847
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 4 cut(s) 842, 1088, 1691, 1757
GluI GCNGC 1 cut(s) 129
GsaI CCCAGC 1 cut(s) 583
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 2 cut(s) 121, 356
HapII CCGG 2 cut(s) 78, 767
HgaI GACGC 1 cut(s) 622
Hin1II CATG 6 cut(s) 271, 644, 785, 856, 1225, 1498
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HinfI GANTC 2 cut(s) 261, 587
HpaII CCGG 2 cut(s) 78, 767
HphI GGTGA 4 cut(s) 322, 1141, 1327, 1537
Hpy166II GTNNAC 4 cut(s) 432, 564, 654, 1354
Hpy188I TCNGA 9 cut(s) 156, 241, 250, 277, 378, 447, 1290, 1467, 1630
Hpy188III TCNNGA 2 cut(s) 92, 178
Hpy8I GTNNAC 4 cut(s) 432, 564, 654, 1354
HpyAV CCTTC 5 cut(s) 647, 726, 905, 1435, 1649
HpyCH4III ACNGT 4 cut(s) 98, 288, 467, 1193
HpyF10VI GCNNNNNNNGC 2 cut(s) 1409, 1433
HpyF3I CTNAG 6 cut(s) 249, 337, 509, 1073, 1250, 1602
Hsp92II CATG 6 cut(s) 271, 644, 785, 856, 1225, 1498
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1696
LweI GCATC 5 cut(s) 103, 390, 430, 964, 1825
MabI ACCWGGT 1 cut(s) 484
MaeI CTAG 4 cut(s) 842, 1088, 1691, 1757
MaeIII GTNAC 4 cut(s) 495, 1134, 1525, 1576
MalI GATC 6 cut(s) 182, 272, 666, 1148, 1432, 1698
MboI GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1696
MboII GAAGA 9 cut(s) 175, 228, 671, 956, 1013, 1316, 1664, 1784, 1793
MfeI CAATTG 1 cut(s) 1829
MflI RGATCY 1 cut(s) 1696
MhlI GDGCHC 3 cut(s) 14, 1356, 1719
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 2 cut(s) 300, 1666
MroXI GAANNNNTTC 1 cut(s) 1620
MseI TTAA 5 cut(s) 615, 1238, 1365, 1397, 1751
MslI CAYNNNNRTG 2 cut(s) 511, 645
MspA1I CMGCKG 3 cut(s) 128, 579, 1436
MspI CCGG 2 cut(s) 78, 767
MspR9I CCNGG 2 cut(s) 300, 486
MunI CAATTG 1 cut(s) 1829
Mva1269I GAATGC 1 cut(s) 1800
MvaI CCWGG 2 cut(s) 300, 486
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 2 cut(s) 1409, 1433
NdeI CATATG 1 cut(s) 1450
NdeII GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1696
NlaIII CATG 6 cut(s) 271, 644, 785, 856, 1225, 1498
NlaIV GGNNCC 1 cut(s) 1500
NmuCI GTSAC 1 cut(s) 1525
OliI CACNNNNGTG 1 cut(s) 511
PaqCI CACCTGC 2 cut(s) 152, 1540
PctI GAATGC 1 cut(s) 1800
PdmI GAANNNNTTC 1 cut(s) 1620
PfeI GAWTC 1 cut(s) 261
PflFI GACNNNGTC 1 cut(s) 590
PflMI CCANNNNNTGG 1 cut(s) 1267
PkrI GCNGC 1 cut(s) 130
PleI GAGTC 1 cut(s) 581
PpsI GAGTC 1 cut(s) 581
Psp6I CCWGG 2 cut(s) 298, 484
PspFI CCCAGC 1 cut(s) 579
PspGI CCWGG 2 cut(s) 298, 484
PspN4I GGNNCC 1 cut(s) 1500
PspPI GGNCC 1 cut(s) 119
PsuI RGATCY 1 cut(s) 1696
PsyI GACNNNGTC 1 cut(s) 590
PvuII CAGCTG 1 cut(s) 579
RsaI GTAC 7 cut(s) 30, 347, 550, 840, 1372, 1380, 1806
RsaNI GTAC 7 cut(s) 29, 346, 549, 839, 1371, 1379, 1805
RseI CAYNNNNRTG 2 cut(s) 511, 645
SacII CCGCGG 1 cut(s) 129
SaqAI TTAA 5 cut(s) 615, 1238, 1365, 1397, 1751
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 6 cut(s) 180, 270, 664, 1146, 1430, 1696
Sau96I GGNCC 1 cut(s) 119
ScaI AGTACT 2 cut(s) 347, 1806
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 2 cut(s) 300, 486
SduI GDGCHC 3 cut(s) 14, 1356, 1719
SexAI ACCWGGT 1 cut(s) 484
SfaNI GCATC 5 cut(s) 103, 390, 430, 964, 1825
SfcI CTRYAG 2 cut(s) 991, 1017
Sfr303I CCGCGG 1 cut(s) 129
SgrBI CCGCGG 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 511, 645
SmlI CTYRAG 1 cut(s) 1800
SmoI CTYRAG 1 cut(s) 1800
SpeI ACTAGT 2 cut(s) 841, 1690
SsiI CCGC 4 cut(s) 126, 128, 216, 1436
SspMI CTAG 4 cut(s) 842, 1088, 1691, 1757
StyD4I CCNGG 2 cut(s) 298, 484
TaaI ACNGT 4 cut(s) 98, 288, 467, 1193
TaqI TCGA 1 cut(s) 384
TatI WGTACW 4 cut(s) 345, 838, 1378, 1804
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 5 cut(s) 615, 1238, 1365, 1397, 1751
Tru9I TTAA 5 cut(s) 615, 1238, 1365, 1397, 1751
TscAI CASTG 7 cut(s) 112, 472, 628, 759, 1081, 1196, 1740
TseFI GTSAC 1 cut(s) 1525
Tsp45I GTSAC 1 cut(s) 1525
TspDTI ATGAA 5 cut(s) 629, 770, 1014, 1210, 1224
TspGWI ACGGA 1 cut(s) 586
TspRI CASTG 7 cut(s) 112, 472, 628, 759, 1081, 1196, 1740
Tth111I GACNNNGTC 1 cut(s) 590
Van91I CCANNNNNTGG 1 cut(s) 1267
VneI GTGCAC 1 cut(s) 1352
XapI RAATTY 2 cut(s) 470, 1414
XcmI CCANNNNNNNNNTGG 2 cut(s) 542, 928
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 1620
XspI CTAG 4 cut(s) 842, 1088, 1691, 1757
ZrmI AGTACT 2 cut(s) 347, 1806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.