pycom02g12340

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012563
Physical Location & Seq
Forward (+)
8896216 .. 8898144
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12340.1

Sequence Viewer

Length: 1929 bp
ATGTACTACAAAACTTCAAGACTTCAAGCTTTTCCTCTGCCAGAAGTCTTCACAGAAAGTAGAAACTTGATCATGGATTGCTCTATGTTCATGTTCTTAGTTTTGTTGCTCTTCACTCCGAAAACCAAAGCCCAACAAAGTTATTTAGAAGACTCGGCTTCTGATTGTGGCGAAAATGTTGGTACATCTCCGGCTTTTCTGTACAGCTGCAGTAGAAAAAAGCAGTCATGCAAGGCTTTCTTGATTTTCCTCTCTCAACCCCCTTATGATTCCGTCTCTGCCATATCGAACCTCACAGCTTCAGACCCGTCTGAGCTTGCGCGGATCAACAATGTCTCGGATCATACTCTCTTGCTGCCTCCAAACAAAGAGGTGATTGTTCCGGTGGTCTGTTCTTGTTCAGATCAGTCCTATGAGGCCAAAGCCTCTTTTGTCATTCCCTCTATAGACCACACATATTATATAATAGCAACTCAGACATATCAGAGTTTGTCTACTTGTAATGCTCTCATGCAACAGAACAATTATAGTGCATTCCACTTGCAGCCTGGCATGAAATTAAAGGTGCCGCTGAGATGTGCTTGTCCCACAAGCAACCAAACTGCCGATGGTACTAAATTTCTAATGACTTACTTGGTCAGCTTGGATGATTCTGTCCCTGACGTGAGTAAGAGATTTAATGTAAGTGTAAAGAGTGTAGCTTATGCAAATAGGTTTACAGAAGATGATCCTCCTTTGCTAGCATTTACACCCATTCTAATTCCACTGTCGGTGGAGCCTTCGAGCTCTCAAACCCACCTGCCTGGGCCACCGCCATTCCTTTCTCCTCCGGTTACTTCTATTCATGGAAATAGAAGATCTAAGGATATACTTATTTGGGTTGGAATTGGAATTGGAATTGGAATTCTTCTGCTAGTTCTCTGCTTGGTTATCTCTGTGGTCTTCTTTCGTTGCCAGAAAAAGGGTTCTCAAAAGAATACAGAAGGAAAGAAGAAAGGGGCACTGGAGGAAGATTTATTTGTTACCATTTCTCAAGTTGATGTAGGGTTGAAAGTCTTCGAATACGGAGAATTGAAACATGCTACGGAGGATTTCAGCGAAAAGAGCTGGTTGGGGGGCTCTGTACATAAGGGGTTTCTCAACGGAAAAGCGCTAGCCATTAAGAAAATGAGCAAAGATGTGACTAAGGAGGTGAACTTACTGAAAAAGGTTAACCATTTCAATCTGATCAGCCTTTTGGGTGCATGTGAACACGGCGGGGATTTGTACCTCATGTATGAGTTCATGGAAAATGGTTCGTTACGAGATTGGATCAAGAAAAACAATTGTCCAGAAGTTCAGAGTTGGGATCGTAGGGTTCAGATCGCTTTGGACATTGCCAATGGGCTTCACTATCTGCACAACTTCACCAGTCCTGCTTACGTGCACAAGGATCTTAGCAGTACCAATGTTCTACTCAACAAAAATTTAAGGGCTAAGATTGCAAATTTCAGCCTCGCACGGTCAGCAGAGAATCAATCGAATGGGAATTCATCTATAAGGTATGCCAGGGTACCAAATGGTTACATGGCACCAGAATATATAGAATATGGATTGGTGACACCTGCAATCGATATCTACGCCTTTGGGGTTGTTATGCTGGAGTTGGTAACAGGAAAAGAAGCTGTATTCTTGCAGGATGGAGTGGAAGTACTTCTTTCAGACACAATCTCTACAACAATGGGAGGAGAAAATGCAGAAGCTCAGCCTGATGGGCTAATAGATCCGAATTTCAATGTAGAGCGCTGCGCAGAGTTTGCAGTACGAATGATAAAGCTAAGTCTTGAATGCTTGGCTCAAGAGCCAGAAAACAGACCAAGAATCCATCAAGTACTCTCGTTCCTGCAAAAAATTCAATTCGATGCAATAAAAGTAGATTCATTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

643

Amino Acids

71.31

Weight (kDa)

6.38

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM1_NFP_LYK PF23446 76 - 133 7.1e-17 NFP/LYK LysM1 domain
LysM2_CERK1_LYK3_4_5 PF23472 138 - 192 1e-20 LYK3/4/5 LysM2 domain
LysM3_LYK4_5 PF23473 207 - 256 7.1e-15 LYK4/5 LysM3 domain
Pkinase PF00069 387 - 625 1.4e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 388 - 627 1.1e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 807, 1612
Acc16I TGCGCA 1 cut(s) 1789
Acc36I ACCTGC 2 cut(s) 807, 1612
Acc65I GGTACC 1 cut(s) 1552
AccB1I GGYRCC 3 cut(s) 565, 1552, 1570
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 322
AciI CCGC 4 cut(s) 322, 569, 812, 1257
AclWI GGATC 7 cut(s) 332, 348, 722, 1319, 1356, 1440, 1757
AcsI RAATTY 7 cut(s) 617, 903, 1465, 1486, 1528, 1768, 1890
AcuI CTGAAG 1 cut(s) 285
AfeI AGCGCT 2 cut(s) 1152, 1784
AfiI CCNNNNNNNGG 1 cut(s) 961
AgsI TTSAA 8 cut(s) 18, 26, 1051, 1075, 1222, 1774, 1826, 1895
AjiI CACGTC 1 cut(s) 664
AjnI CCWGG 3 cut(s) 547, 802, 1547
Alw21I GWGCWC 2 cut(s) 788, 1428
Alw26I GTCTC 2 cut(s) 280, 340
Alw44I GTGCAC 1 cut(s) 1424
AlwI GGATC 7 cut(s) 332, 348, 722, 1319, 1356, 1440, 1757
Aor51HI AGCGCT 2 cut(s) 1152, 1784
AoxI GGCC 2 cut(s) 417, 806
ApaLI GTGCAC 1 cut(s) 1424
ApeKI GCWGC 4 cut(s) 207, 355, 544, 1785
ApoI RAATTY 7 cut(s) 617, 903, 1465, 1486, 1528, 1768, 1890
Asp700I GAANNNNTTC 2 cut(s) 1055, 1692
Asp718I GGTACC 1 cut(s) 1552
AspLEI GCGC 4 cut(s) 322, 1153, 1785, 1790
AspS9I GGNCC 1 cut(s) 806
AsuHPI GGTGA 4 cut(s) 385, 1204, 1399, 1609
AsuII TTCGAA 1 cut(s) 1059
AsuNHI GCTAGC 2 cut(s) 739, 1153
BaeGI GKGCMC 2 cut(s) 1003, 1428
BanI GGYRCC 3 cut(s) 565, 1552, 1570
BanII GRGCYC 2 cut(s) 788, 1121
BbsI GAAGAC 4 cut(s) 40, 156, 934, 1048
Bbv12I GWGCWC 2 cut(s) 788, 1428
BbvI GCAGC 4 cut(s) 194, 342, 556, 1772
BccI CCATC 4 cut(s) 602, 1673, 1745, 1872
BceAI ACGGC 1 cut(s) 1270
BciT130I CCWGG 3 cut(s) 549, 804, 1549
BclI TGATCA 2 cut(s) 69, 1227
BcoDI GTCTC 2 cut(s) 280, 340
BfaI CTAG 3 cut(s) 740, 914, 1154
BfmI CTRYAG 2 cut(s) 208, 444
BfoI RGCGCY 2 cut(s) 1154, 1786
BfuAI ACCTGC 2 cut(s) 807, 1612
BglI GCCNNNNNGGC 1 cut(s) 1753
BglII AGATCT 1 cut(s) 857
BisI GCNGC 5 cut(s) 208, 356, 545, 569, 1786
BlpI GCTNAGC 1 cut(s) 1743
BlsI GCNGC 5 cut(s) 209, 357, 546, 570, 1787
BmcAI AGTACT 2 cut(s) 1692, 1872
Bme1390I CCNGG 3 cut(s) 549, 804, 1549
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 4 cut(s) 567, 777, 1554, 1572
BmrFI CCNGG 3 cut(s) 549, 804, 1549
BmsI GCATC 1 cut(s) 1891
BmtI GCTAGC 2 cut(s) 743, 1157
BpiI GAAGAC 4 cut(s) 40, 156, 934, 1048
BpmI CTGGAG 2 cut(s) 1025, 1661
Bpu1102I GCTNAGC 1 cut(s) 1743
Bpu14I TTCGAA 1 cut(s) 1059
BpuEI CTTGAG 2 cut(s) 1017, 1821
Bsa29I ATCGAT 1 cut(s) 1611
BsaAI YACGTR 1 cut(s) 1423
BsaJI CCNNGG 2 cut(s) 803, 1548
BsaWI WCCGGW 2 cut(s) 382, 829
BsaXI ACNNNNNCTCC 2 cut(s) 1674, 1704
Bsc4I CCNNNNNNNGG 1 cut(s) 961
Bse1I ACTGG 2 cut(s) 1008, 1410
Bse3DI GCAATG 1 cut(s) 1374
BseBI CCWGG 3 cut(s) 549, 804, 1549
BseCI ATCGAT 1 cut(s) 1611
BseDI CCNNGG 2 cut(s) 803, 1548
BseGI GGATG 2 cut(s) 652, 1684
BseLI CCNNNNNNNGG 1 cut(s) 961
BseMI GCAATG 1 cut(s) 1374
BseMII CTCAG 4 cut(s) 303, 488, 563, 1757
BseNI ACTGG 2 cut(s) 1008, 1410
BseRI GAGGAG 2 cut(s) 816, 1740
BseSI GKGCMC 2 cut(s) 1003, 1428
BseXI GCAGC 4 cut(s) 194, 342, 556, 1772
BsgI GTGCAG 1 cut(s) 1382
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 419, 808
BshNI GGYRCC 3 cut(s) 565, 1552, 1570
BshVI ATCGAT 1 cut(s) 1611
BsiHKAI GWGCWC 2 cut(s) 788, 1428
BsiSI CCGG 3 cut(s) 191, 383, 830
BslFI GGGAC 2 cut(s) 570, 641
BslI CCNNNNNNNGG 1 cut(s) 961
BsmAI GTCTC 2 cut(s) 280, 340
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 2 cut(s) 570, 641
BsmI GAATGC 2 cut(s) 533, 1832
BsnI GGCC 2 cut(s) 419, 808
Bsp119I TTCGAA 1 cut(s) 1059
Bsp1286I GDGCHC 4 cut(s) 788, 1003, 1121, 1428
Bsp1407I TGTACA 2 cut(s) 201, 1123
Bsp1720I GCTNAGC 1 cut(s) 1743
BspACI CCGC 4 cut(s) 322, 569, 812, 1257
BspANI GGCC 2 cut(s) 419, 808
BspCNI CTCAG 4 cut(s) 304, 487, 564, 1756
BspDI ATCGAT 1 cut(s) 1611
BspFNI CGCG 1 cut(s) 322
BspLI GGNNCC 4 cut(s) 567, 777, 1554, 1572
BspMAI CTGCAG 1 cut(s) 212
BspMI ACCTGC 2 cut(s) 807, 1612
BspOI GCTAGC 2 cut(s) 743, 1157
BspPI GGATC 7 cut(s) 332, 348, 722, 1319, 1356, 1440, 1757
BspQI GCTCTTC 1 cut(s) 116
BspT104I TTCGAA 1 cut(s) 1059
BspT107I GGYRCC 3 cut(s) 565, 1552, 1570
BsrDI GCAATG 1 cut(s) 1374
BsrGI TGTACA 2 cut(s) 201, 1123
BsrI ACTGG 2 cut(s) 1008, 1410
BssECI CCNNGG 2 cut(s) 803, 1548
Bst2UI CCWGG 3 cut(s) 549, 804, 1549
Bst4CI ACNGT 2 cut(s) 768, 1503
Bst6I CTCTTC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 1796
BstAUI TGTACA 2 cut(s) 201, 1123
BstBAI YACGTR 1 cut(s) 1423
BstBI TTCGAA 1 cut(s) 1059
BstC8I GCNNGC 3 cut(s) 318, 741, 1155
BstF5I GGATG 2 cut(s) 652, 1684
BstFNI CGCG 1 cut(s) 322
BstH2I RGCGCY 2 cut(s) 1154, 1786
BstHHI GCGC 4 cut(s) 322, 1153, 1785, 1790
BstMAI GTCTC 2 cut(s) 280, 340
BstMWI GCNNNNNNNGC 5 cut(s) 1104, 1481, 1505, 1753, 1796
BstNI CCWGG 3 cut(s) 549, 804, 1549
BstNSI RCATGY 2 cut(s) 1082, 1248
BstSCI CCNGG 3 cut(s) 547, 802, 1547
BstSFI CTRYAG 2 cut(s) 208, 444
BstSLI GKGCMC 2 cut(s) 1003, 1428
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 4 cut(s) 194, 342, 556, 1772
BstV2I GAAGAC 4 cut(s) 40, 156, 934, 1048
BstX2I RGATCY 3 cut(s) 857, 1432, 1762
BstXI CCANNNNNNTGG 1 cut(s) 803
BstYI RGATCY 3 cut(s) 857, 1432, 1762
Bsu15I ATCGAT 1 cut(s) 1611
BsuRI GGCC 2 cut(s) 419, 808
BsuTUI ATCGAT 1 cut(s) 1611
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 2 cut(s) 652, 1684
BtsIMutI CAGTG 2 cut(s) 764, 1001
BveI ACCTGC 2 cut(s) 807, 1612
Cac8I GCNNGC 3 cut(s) 318, 741, 1155
CfoI GCGC 4 cut(s) 322, 1153, 1785, 1790
Cfr13I GGNCC 1 cut(s) 806
ClaI ATCGAT 1 cut(s) 1611
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 786
Eco24I GRGCYC 2 cut(s) 788, 1121
Eco32I GATATC 1 cut(s) 1615
Eco47III AGCGCT 2 cut(s) 1152, 1784
Eco53kI GAGCTC 1 cut(s) 786
Eco57I CTGAAG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 786
EcoRI GAATTC 2 cut(s) 903, 1528
EcoRII CCWGG 3 cut(s) 547, 802, 1547
EcoRV GATATC 1 cut(s) 1615
EcoT38I GRGCYC 2 cut(s) 788, 1121
Esp3I CGTCTC 1 cut(s) 280
FalI AAGNNNNNCTT 4 cut(s) 224, 256, 1680, 1712
FaqI GGGAC 2 cut(s) 570, 641
FauI CCCGC 1 cut(s) 1250
FbaI TGATCA 2 cut(s) 69, 1227
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 5 cut(s) 208, 356, 545, 569, 1786
FokI GGATG 2 cut(s) 659, 1691
FriOI GRGCYC 2 cut(s) 788, 1121
Fsp4HI GCNGC 5 cut(s) 208, 356, 545, 569, 1786
FspBI CTAG 3 cut(s) 740, 914, 1154
FspI TGCGCA 1 cut(s) 1789
GlaI GCGC 4 cut(s) 321, 1152, 1784, 1789
GluI GCNGC 5 cut(s) 208, 356, 545, 569, 1786
GsuI CTGGAG 2 cut(s) 1025, 1661
HaeII RGCGCY 2 cut(s) 1154, 1786
HaeIII GGCC 2 cut(s) 419, 808
HapII CCGG 3 cut(s) 191, 383, 830
HhaI GCGC 4 cut(s) 322, 1153, 1785, 1790
Hin6I GCGC 4 cut(s) 320, 1151, 1783, 1788
HinP1I GCGC 4 cut(s) 320, 1151, 1783, 1788
HincII GTYRAC 1 cut(s) 1213
HindII GTYRAC 1 cut(s) 1213
HindIII AAGCTT 1 cut(s) 27
HinfI GANTC 6 cut(s) 152, 269, 650, 1513, 1860, 1916
HpaI GTTAAC 1 cut(s) 1213
HpaII CCGG 3 cut(s) 191, 383, 830
HphI GGTGA 4 cut(s) 385, 1204, 1399, 1609
Hpy166II GTNNAC 6 cut(s) 495, 717, 1195, 1213, 1250, 1426
Hpy188III TCNNGA 6 cut(s) 18, 241, 1315, 1331, 1823, 1838
Hpy8I GTNNAC 6 cut(s) 495, 717, 1195, 1213, 1250, 1426
HpyAV CCTTC 2 cut(s) 789, 977
HpyCH4III ACNGT 2 cut(s) 768, 1503
HpyCH4IV ACGT 2 cut(s) 663, 1422
HpyF10VI GCNNNNNNNGC 5 cut(s) 1104, 1481, 1505, 1753, 1796
HpySE526I ACGT 2 cut(s) 663, 1422
HspAI GCGC 4 cut(s) 320, 1151, 1783, 1788
KpnI GGTACC 1 cut(s) 1556
Ksp22I TGATCA 2 cut(s) 69, 1227
KspAI GTTAAC 1 cut(s) 1213
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 4 cut(s) 194, 342, 556, 1772
LweI GCATC 1 cut(s) 1891
MaeI CTAG 3 cut(s) 740, 914, 1154
MaeII ACGT 2 cut(s) 663, 1422
MaeIII GTNAC 7 cut(s) 832, 1021, 1180, 1299, 1562, 1597, 1648
MfeI CAATTG 1 cut(s) 1324
MflI RGATCY 3 cut(s) 857, 1432, 1762
MhlI GDGCHC 4 cut(s) 788, 1003, 1121, 1428
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 862
MroXI GAANNNNTTC 2 cut(s) 1055, 1692
MseI TTAA 5 cut(s) 560, 678, 1161, 1212, 1469
MspA1I CMGCKG 2 cut(s) 207, 571
MspI CCGG 3 cut(s) 191, 383, 830
MspR9I CCNGG 3 cut(s) 549, 804, 1549
MteI GCGCNGCGC 1 cut(s) 1786
MunI CAATTG 1 cut(s) 1324
Mva1269I GAATGC 2 cut(s) 533, 1832
MvaI CCWGG 3 cut(s) 549, 804, 1549
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 5 cut(s) 1104, 1481, 1505, 1753, 1796
NheI GCTAGC 2 cut(s) 739, 1153
NlaIV GGNNCC 4 cut(s) 567, 777, 1554, 1572
NmeAIII GCCGAG 1 cut(s) 134
NmuCI GTSAC 2 cut(s) 1180, 1597
NsbI TGCGCA 1 cut(s) 1789
NspI RCATGY 2 cut(s) 1082, 1248
NspV TTCGAA 1 cut(s) 1059
PaqCI CACCTGC 2 cut(s) 807, 1612
PciSI GCTCTTC 1 cut(s) 116
PctI GAATGC 2 cut(s) 533, 1832
PdmI GAANNNNTTC 2 cut(s) 1055, 1692
PfeI GAWTC 5 cut(s) 269, 650, 1513, 1860, 1916
PkrI GCNGC 5 cut(s) 209, 357, 546, 570, 1787
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Ppu21I YACGTR 1 cut(s) 1423
Psp124BI GAGCTC 1 cut(s) 788
Psp6I CCWGG 3 cut(s) 547, 802, 1547
PspGI CCWGG 3 cut(s) 547, 802, 1547
PspN4I GGNNCC 4 cut(s) 567, 777, 1554, 1572
PspPI GGNCC 1 cut(s) 806
PstI CTGCAG 1 cut(s) 212
PsuI RGATCY 3 cut(s) 857, 1432, 1762
PvuII CAGCTG 1 cut(s) 207
SacI GAGCTC 1 cut(s) 788
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 5 cut(s) 560, 678, 1161, 1212, 1469
SatI GCNGC 5 cut(s) 208, 356, 545, 569, 1786
Sau96I GGNCC 1 cut(s) 806
ScaI AGTACT 2 cut(s) 1692, 1872
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 3 cut(s) 549, 804, 1549
SduI GDGCHC 4 cut(s) 788, 1003, 1121, 1428
SfaNI GCATC 1 cut(s) 1891
SfcI CTRYAG 2 cut(s) 208, 444
SfuI TTCGAA 1 cut(s) 1059
SmlI CTYRAG 2 cut(s) 1032, 1836
SmoI CTYRAG 2 cut(s) 1032, 1836
SsiI CCGC 4 cut(s) 322, 569, 812, 1257
SspMI CTAG 3 cut(s) 740, 914, 1154
SstI GAGCTC 1 cut(s) 788
StyD4I CCNGG 3 cut(s) 547, 802, 1547
TaaI ACNGT 2 cut(s) 768, 1503
TaiI ACGT 2 cut(s) 666, 1425
TaqI TCGA 6 cut(s) 287, 782, 1059, 1520, 1611, 1899
TatI WGTACW 5 cut(s) 3, 201, 1123, 1690, 1870
TauI GCSGC 1 cut(s) 571
TfiI GAWTC 5 cut(s) 269, 650, 1513, 1860, 1916
Tru1I TTAA 5 cut(s) 560, 678, 1161, 1212, 1469
Tru9I TTAA 5 cut(s) 560, 678, 1161, 1212, 1469
TscAI CASTG 2 cut(s) 771, 1008
TseFI GTSAC 2 cut(s) 1180, 1597
TseI GCWGC 4 cut(s) 207, 355, 544, 1785
Tsp45I GTSAC 2 cut(s) 1180, 1597
TspDTI ATGAA 6 cut(s) 79, 569, 833, 1273, 1521, 1908
TspGWI ACGGA 4 cut(s) 262, 1080, 1100, 1158
TspRI CASTG 2 cut(s) 771, 1008
VneI GTGCAC 1 cut(s) 1424
XapI RAATTY 7 cut(s) 617, 903, 1465, 1486, 1528, 1768, 1890
XceI RCATGY 2 cut(s) 1082, 1248
XcmI CCANNNNNNNNNTGG 2 cut(s) 545, 605
XmiI GTMKAC 1 cut(s) 494
XmnI GAANNNNTTC 2 cut(s) 1055, 1692
XspI CTAG 3 cut(s) 740, 914, 1154
ZrmI AGTACT 2 cut(s) 1692, 1872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.