FvH4_1g19690

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006547
Physical Location & Seq
Forward (+)
11910463 .. 11913861
3399 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19690.t2

Sequence Viewer

Length: 666 bp
ATGGCGGGATCCTTGTCTCTTCTCAGAAGGGTTTATCTTTCCCTCTATAACTGGGCCGTTTTCATCGGATGGTTTCAGGTGCTGTATCTTGCTGTGAAGACGCTTTGGGAATCGGGCCATGAGGAGGTTTACAATGCCGTGGAGCGCCCACTTCAGTTTGCTCAATCCGCTGCGTTTTTGGAGATTCTTCATGGCTTAGTGGGTTTGGTGAGATCTCCTATAACAGCCACACTGCCGCAGATTAGTTCGAGGTTGTTTCTGATTTGGGGGATTCTGTGGAGCTTTCCTGAGGTTCAGAGTCATGTGCTTGTGAGCTCTTTGGTCATCAGCTGGTCCATCACTGAGATTATTCGTTACTCTTTCTATGGCATGAAGGAGTCTCTTGGTTTCTCACCTTCATGGCTCCAGTGGCTCAGGTACAGCACCTTCATATTGTTGTATCCAATTGGGATCTCCAGTGAAGTTGGCTTAACATATCTTGCCCTGCCACACATTAAGAGTTCTGAGAAGTATTGTATAAGGATGCCAAACAAGTGGAATTTCTCTCTCGAGTACTTCAATGTTGCAGTTATAGCCATCGCATTCTATATCCCAGGCAGTCCTCACTTGTACCGGTATATGCTCGGGCAGAGGAAGAAAGTTCTTGCAAAATCGAAAGCGGAGTAG

Protein Analysis

222

Amino Acids

25.44

Weight (kDa)

9.47

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTPLA PF04387 55 - 199 1.6e-50 Protein tyrosine phosphatase-like protein, PTPLA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 168, 236, 659
AclWI GGATC 3 cut(s) 3, 16, 458
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 1 cut(s) 137
AfaI GTAC 3 cut(s) 419, 554, 611
AfiI CCNNNNNNNGG 1 cut(s) 124
AgeI ACCGGT 1 cut(s) 612
AgsI TTSAA 1 cut(s) 559
AjnI CCWGG 1 cut(s) 592
AluBI AGCT 3 cut(s) 282, 315, 330
AluI AGCT 3 cut(s) 282, 315, 330
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 2 cut(s) 21, 384
AlwI GGATC 3 cut(s) 3, 16, 458
AlwNI CAGNNNCTG 1 cut(s) 82
Ama87I CYCGRG 2 cut(s) 548, 623
AoxI GGCC 2 cut(s) 54, 115
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 538
AsiGI ACCGGT 1 cut(s) 612
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 3 cut(s) 54, 115, 333
AsuHPI GGTGA 2 cut(s) 220, 384
AvaI CYCGRG 2 cut(s) 548, 623
AvaII GGWCC 1 cut(s) 333
AxyI CCTNAGG 1 cut(s) 288
BamHI GGATCC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 317
BarI GAAGNNNNNNTAC 2 cut(s) 410, 442
BbsI GAAGAC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 317
BbvI GCAGC 1 cut(s) 157
BccI CCATC 3 cut(s) 63, 344, 584
BceAI ACGGC 2 cut(s) 41, 122
BciT130I CCWGG 1 cut(s) 594
BciVI GTATCC 1 cut(s) 450
BcoDI GTCTC 2 cut(s) 21, 384
BfoI RGCGCY 1 cut(s) 148
BfuI GTATCC 1 cut(s) 450
BglII AGATCT 1 cut(s) 212
BisI GCNGC 2 cut(s) 171, 236
BlsI GCNGC 2 cut(s) 172, 237
BmcAI AGTACT 1 cut(s) 554
Bme1390I CCNGG 1 cut(s) 594
Bme18I GGWCC 1 cut(s) 333
BmeT110I CYCGRG 2 cut(s) 548, 623
BmgT120I GGNCC 3 cut(s) 54, 115, 333
BmiI GGNNCC 2 cut(s) 10, 404
BmrFI CCNGG 1 cut(s) 594
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 61
BpiI GAAGAC 1 cut(s) 104
BpmI CTGGAG 2 cut(s) 389, 439
Bpu10I CCTNAGC 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 138, 592
BsaWI WCCGGW 1 cut(s) 612
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse118I RCCGGY 1 cut(s) 612
Bse1I ACTGG 3 cut(s) 56, 406, 456
Bse21I CCTNAGG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 594
BseDI CCNNGG 2 cut(s) 138, 592
BseGI GGATG 2 cut(s) 74, 528
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 5 cut(s) 37, 279, 333, 427, 495
BseNI ACTGG 3 cut(s) 56, 406, 456
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 157
BshFI GGCC 2 cut(s) 56, 117
BshTI ACCGGT 1 cut(s) 612
BsiHKAI GWGCWC 1 cut(s) 317
BsiHKCI CYCGRG 2 cut(s) 548, 623
BsiSI CCGG 1 cut(s) 613
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 2 cut(s) 21, 384
BsmI GAATGC 1 cut(s) 581
BsnI GGCC 2 cut(s) 56, 117
BsoBI CYCGRG 2 cut(s) 548, 623
Bsp1286I GDGCHC 1 cut(s) 317
Bsp143I GATC 3 cut(s) 8, 212, 450
BspACI CCGC 4 cut(s) 5, 168, 236, 659
BspANI GGCC 2 cut(s) 56, 117
BspCNI CTCAG 5 cut(s) 36, 280, 334, 426, 496
BspLI GGNNCC 2 cut(s) 10, 404
BspPI GGATC 3 cut(s) 3, 16, 458
BsrFI RCCGGY 1 cut(s) 612
BsrI ACTGG 3 cut(s) 56, 406, 456
BssAI RCCGGY 1 cut(s) 612
BssECI CCNNGG 2 cut(s) 138, 592
BssMI GATC 3 cut(s) 8, 212, 450
Bst2UI CCWGG 1 cut(s) 594
Bst6I CTCTTC 1 cut(s) 24
BstDEI CTNAG 6 cut(s) 23, 196, 288, 342, 413, 504
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 2 cut(s) 74, 528
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 3 cut(s) 11, 215, 453
BstMAI GTCTC 2 cut(s) 21, 384
BstMBI GATC 3 cut(s) 8, 212, 450
BstMWI GCNNNNNNNGC 3 cut(s) 167, 409, 572
BstNI CCWGG 1 cut(s) 594
BstSCI CCNGG 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 3 cut(s) 8, 212, 450
BstXI CCANNNNNNTGG 1 cut(s) 534
BstYI RGATCY 3 cut(s) 8, 212, 450
Bsu36I CCTNAGG 1 cut(s) 288
BsuI GTATCC 1 cut(s) 450
BsuRI GGCC 2 cut(s) 56, 117
BtgI CCRYGG 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 562
BtsCI GGATG 2 cut(s) 74, 528
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 4 cut(s) 230, 339, 413, 463
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 1 cut(s) 612
Cfr13I GGNCC 3 cut(s) 54, 115, 333
CseI GACGC 1 cut(s) 109
Csp6I GTAC 3 cut(s) 418, 553, 610
CspAI ACCGGT 1 cut(s) 612
CviAII CATG 5 cut(s) 119, 191, 302, 370, 399
CviQI GTAC 3 cut(s) 418, 553, 610
DdeI CTNAG 6 cut(s) 23, 196, 288, 342, 413, 504
DpnI GATC 3 cut(s) 10, 214, 452
DpnII GATC 3 cut(s) 8, 212, 450
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 315
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 288
Eco88I CYCGRG 2 cut(s) 548, 623
EcoICRI GAGCTC 1 cut(s) 315
EcoRII CCWGG 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 317
FaeI CATG 5 cut(s) 122, 194, 305, 373, 402
FatI CATG 5 cut(s) 118, 190, 301, 369, 398
Fnu4HI GCNGC 2 cut(s) 171, 236
FokI GGATG 2 cut(s) 81, 535
FriOI GRGCYC 1 cut(s) 317
Fsp4HI GCNGC 2 cut(s) 171, 236
GlaI GCGC 1 cut(s) 146
GluI GCNGC 2 cut(s) 171, 236
GsuI CTGGAG 2 cut(s) 389, 439
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 2 cut(s) 56, 117
HapII CCGG 1 cut(s) 613
HgaI GACGC 1 cut(s) 109
HhaI GCGC 1 cut(s) 147
Hin1II CATG 5 cut(s) 122, 194, 305, 373, 402
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HinfI GANTC 5 cut(s) 110, 184, 271, 298, 377
HpaII CCGG 1 cut(s) 613
HphI GGTGA 2 cut(s) 220, 384
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 5 cut(s) 26, 68, 261, 297, 505
Hpy188III TCNNGA 2 cut(s) 287, 548
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 4 cut(s) 21, 367, 405, 436
HpyCH4V TGCA 2 cut(s) 566, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 409, 572
HpyF3I CTNAG 6 cut(s) 23, 196, 288, 342, 413, 504
Hsp92II CATG 5 cut(s) 122, 194, 305, 373, 402
HspAI GCGC 1 cut(s) 145
Kzo9I GATC 3 cut(s) 8, 212, 450
LmnI GCTCC 3 cut(s) 142, 279, 408
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 1 cut(s) 513
MaeIII GTNAC 1 cut(s) 353
MalI GATC 3 cut(s) 10, 214, 452
MboI GATC 3 cut(s) 8, 212, 450
MboII GAAGA 4 cut(s) 11, 109, 179, 646
MfeI CAATTG 1 cut(s) 444
MflI RGATCY 3 cut(s) 8, 212, 450
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 2 cut(s) 444, 538
MlyI GAGTC 2 cut(s) 307, 386
MnlI CCTC 7 cut(s) 53, 115, 118, 243, 283, 612, 624
MseI TTAA 2 cut(s) 470, 495
MslI CAYNNNNRTG 1 cut(s) 397
MspA1I CMGCKG 2 cut(s) 170, 330
MspI CCGG 1 cut(s) 613
MspR9I CCNGG 1 cut(s) 594
MunI CAATTG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 581
MvaI CCWGG 1 cut(s) 594
MwoI GCNNNNNNNGC 3 cut(s) 167, 409, 572
NdeII GATC 3 cut(s) 8, 212, 450
NlaIII CATG 5 cut(s) 122, 194, 305, 373, 402
NlaIV GGNNCC 2 cut(s) 10, 404
PaeR7I CTCGAG 1 cut(s) 548
PctI GAATGC 1 cut(s) 581
PfeI GAWTC 3 cut(s) 110, 184, 271
PinAI ACCGGT 1 cut(s) 612
PkrI GCNGC 2 cut(s) 172, 237
PleI GAGTC 2 cut(s) 306, 385
PpsI GAGTC 2 cut(s) 306, 385
Psp124BI GAGCTC 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 592
PspN4I GGNNCC 2 cut(s) 10, 404
PspPI GGNCC 3 cut(s) 54, 115, 333
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 3 cut(s) 8, 212, 450
PvuII CAGCTG 1 cut(s) 330
RsaI GTAC 3 cut(s) 419, 554, 611
RsaNI GTAC 3 cut(s) 418, 553, 610
RseI CAYNNNNRTG 1 cut(s) 397
SacI GAGCTC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 470, 495
SatI GCNGC 2 cut(s) 171, 236
Sau3AI GATC 3 cut(s) 8, 212, 450
Sau96I GGNCC 3 cut(s) 54, 115, 333
ScaI AGTACT 1 cut(s) 554
SchI GAGTC 2 cut(s) 307, 386
ScrFI CCNGG 1 cut(s) 594
SduI GDGCHC 1 cut(s) 317
SfaNI GCATC 1 cut(s) 513
Sfr274I CTCGAG 1 cut(s) 548
SinI GGWCC 1 cut(s) 333
SlaI CTCGAG 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 397
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 2 cut(s) 444, 538
SsiI CCGC 4 cut(s) 5, 168, 236, 659
SstI GAGCTC 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 592
TaqI TCGA 3 cut(s) 248, 549, 653
TasI AATT 2 cut(s) 444, 538
TatI WGTACW 1 cut(s) 552
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 3 cut(s) 110, 184, 271
Tru1I TTAA 2 cut(s) 470, 495
Tru9I TTAA 2 cut(s) 470, 495
TscAI CASTG 4 cut(s) 237, 346, 413, 463
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 5 cut(s) 52, 179, 386, 387, 418
TspRI CASTG 4 cut(s) 237, 346, 413, 463
VpaK11BI GGWCC 1 cut(s) 333
XapI RAATTY 1 cut(s) 538
XhoI CTCGAG 1 cut(s) 548
ZrmI AGTACT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.