Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G10480 AT5G10480 AT5G10480 |
| fragaria_vesca | FvH4_1g19690 FvH4_1g19690 |
| malus_domestica | MD01G1038800.v1.1 MD04G1039300.v1.1 MD15G1312800.v1.1 MD15G1313000.v1.1 |
| prunus_persica | Prupe.6G201300_v2.0.a1 Prupe.6G201300_v2.0.a1 Prupe.6G201300_v2.0.a1 |
| pyrus_communis | pycom01g06670 |
| rosa_chinensis | RchiOBHm_Chr2g0111251 |
| rosa_laevigata | RLG00000017879 |
| rosa_multiflora | Rmu_co8061266.1_g000001 Rmu_sc0001721.1_g000014 Rmu_sc0003055.1_g000014 |
| rosa_roxburghii | Rroxscaffold_2G00132570 |
| rosa_rugosa | Rorug02G0172000 Rorug02G0172100 |
| rosa_samantha | Rh2AG225200 Rh2BG237000 Rh2CG228000 Rh2DG232600 |
| rosa_wichuraiana | Rw2G017380 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 67 |
| AccB1I | GGYRCC | 1 cut(s) | 27 |
| AccBSI | CCGCTC | 1 cut(s) | 20 |
| AciI | CCGC | 6 cut(s) | 5, 18, 168, 171, 236, 659 |
| AcyI | GRCGYC | 1 cut(s) | 28 |
| AfaI | GTAC | 2 cut(s) | 81, 611 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 24, 242 |
| AflIII | ACRYGT | 1 cut(s) | 606 |
| AgeI | ACCGGT | 1 cut(s) | 612 |
| AgsI | TTSAA | 3 cut(s) | 143, 249, 648 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 297 |
| AjnI | CCWGG | 1 cut(s) | 592 |
| AluBI | AGCT | 2 cut(s) | 315, 641 |
| AluI | AGCT | 2 cut(s) | 315, 641 |
| Alw21I | GWGCWC | 1 cut(s) | 317 |
| Alw26I | GTCTC | 1 cut(s) | 284 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 82 |
| AoxI | GGCC | 1 cut(s) | 115 |
| ApeKI | GCWGC | 2 cut(s) | 563, 597 |
| AsiGI | ACCGGT | 1 cut(s) | 612 |
| AspLEI | GCGC | 1 cut(s) | 30 |
| AspS9I | GGNCC | 1 cut(s) | 54 |
| AvaII | GGWCC | 1 cut(s) | 54 |
| BanI | GGYRCC | 1 cut(s) | 27 |
| BanII | GRGCYC | 1 cut(s) | 317 |
| BbsI | GAAGAC | 1 cut(s) | 52 |
| Bbv12I | GWGCWC | 1 cut(s) | 317 |
| BbvI | GCAGC | 2 cut(s) | 550, 609 |
| BccI | CCATC | 1 cut(s) | 63 |
| BceAI | ACGGC | 2 cut(s) | 122, 158 |
| BciT130I | CCWGG | 1 cut(s) | 594 |
| BciVI | GTATCC | 1 cut(s) | 450 |
| BcoDI | GTCTC | 1 cut(s) | 284 |
| BfaI | CTAG | 2 cut(s) | 197, 578 |
| BfoI | RGCGCY | 1 cut(s) | 31 |
| BfuAI | ACCTGC | 1 cut(s) | 67 |
| BfuI | GTATCC | 1 cut(s) | 450 |
| BglII | AGATCT | 1 cut(s) | 212 |
| BisI | GCNGC | 5 cut(s) | 171, 236, 564, 598, 660 |
| BlsI | GCNGC | 5 cut(s) | 172, 237, 565, 599, 661 |
| Bme1390I | CCNGG | 1 cut(s) | 594 |
| Bme18I | GGWCC | 1 cut(s) | 54 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 297 |
| BmgT120I | GGNCC | 1 cut(s) | 54 |
| BmiI | GGNNCC | 1 cut(s) | 29 |
| BmrFI | CCNGG | 1 cut(s) | 594 |
| BmsI | GCATC | 2 cut(s) | 459, 513 |
| BpiI | GAAGAC | 1 cut(s) | 52 |
| BpmI | CTGGAG | 1 cut(s) | 298 |
| Bpu10I | CCTNAGC | 1 cut(s) | 413 |
| BpuEI | CTTGAG | 1 cut(s) | 80 |
| BsaHI | GRCGYC | 1 cut(s) | 28 |
| BsaJI | CCNNGG | 2 cut(s) | 64, 592 |
| BsaWI | WCCGGW | 1 cut(s) | 612 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 24, 242 |
| Bse118I | RCCGGY | 1 cut(s) | 612 |
| Bse1I | ACTGG | 2 cut(s) | 56, 451 |
| BseBI | CCWGG | 1 cut(s) | 594 |
| BseDI | CCNNGG | 2 cut(s) | 64, 592 |
| BseGI | GGATG | 2 cut(s) | 74, 528 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 24, 242 |
| BseMII | CTCAG | 5 cut(s) | 37, 279, 308, 333, 427 |
| BseNI | ACTGG | 2 cut(s) | 56, 451 |
| BseXI | GCAGC | 2 cut(s) | 550, 609 |
| BshFI | GGCC | 1 cut(s) | 117 |
| BshNI | GGYRCC | 1 cut(s) | 27 |
| BshTI | ACCGGT | 1 cut(s) | 612 |
| BsiHKAI | GWGCWC | 1 cut(s) | 317 |
| BsiSI | CCGG | 1 cut(s) | 613 |
| BslFI | GGGAC | 1 cut(s) | 575 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 24, 242 |
| BsmAI | GTCTC | 1 cut(s) | 284 |
| BsmFI | GGGAC | 1 cut(s) | 575 |
| BsnI | GGCC | 1 cut(s) | 117 |
| Bsp1286I | GDGCHC | 1 cut(s) | 317 |
| Bsp143I | GATC | 1 cut(s) | 212 |
| BspACI | CCGC | 6 cut(s) | 5, 18, 168, 171, 236, 659 |
| BspANI | GGCC | 1 cut(s) | 117 |
| BspCNI | CTCAG | 5 cut(s) | 36, 280, 307, 334, 426 |
| BspLI | GGNNCC | 1 cut(s) | 29 |
| BspMI | ACCTGC | 1 cut(s) | 67 |
| BspT107I | GGYRCC | 1 cut(s) | 27 |
| BsrBI | CCGCTC | 1 cut(s) | 20 |
| BsrFI | RCCGGY | 1 cut(s) | 612 |
| BsrI | ACTGG | 2 cut(s) | 56, 451 |
| BssAI | RCCGGY | 1 cut(s) | 612 |
| BssECI | CCNNGG | 2 cut(s) | 64, 592 |
| BssMI | GATC | 1 cut(s) | 212 |
| BssNI | GRCGYC | 1 cut(s) | 28 |
| Bst2UI | CCWGG | 1 cut(s) | 594 |
| Bst4CI | ACNGT | 2 cut(s) | 58, 84 |
| BstACI | GRCGYC | 1 cut(s) | 28 |
| BstDEI | CTNAG | 6 cut(s) | 23, 288, 294, 342, 413, 623 |
| BstF5I | GGATG | 2 cut(s) | 74, 528 |
| BstH2I | RGCGCY | 1 cut(s) | 31 |
| BstHHI | GCGC | 1 cut(s) | 30 |
| BstKTI | GATC | 1 cut(s) | 215 |
| BstMAI | GTCTC | 1 cut(s) | 284 |
| BstMBI | GATC | 1 cut(s) | 212 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 17, 167, 409, 572 |
| BstNI | CCWGG | 1 cut(s) | 594 |
| BstNSI | RCATGY | 2 cut(s) | 128, 610 |
| BstSCI | CCNGG | 1 cut(s) | 592 |
| BstV1I | GCAGC | 2 cut(s) | 550, 609 |
| BstV2I | GAAGAC | 1 cut(s) | 52 |
| BstX2I | RGATCY | 1 cut(s) | 212 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 534 |
| BstYI | RGATCY | 1 cut(s) | 212 |
| BsuI | GTATCC | 1 cut(s) | 450 |
| BsuRI | GGCC | 1 cut(s) | 117 |
| BtsCI | GGATG | 2 cut(s) | 74, 528 |
| BtsI | GCAGTG | 1 cut(s) | 230 |
| BtsIMutI | CAGTG | 2 cut(s) | 230, 339 |
| BveI | ACCTGC | 1 cut(s) | 67 |
| CaiI | CAGNNNCTG | 1 cut(s) | 82 |
| CfoI | GCGC | 1 cut(s) | 30 |
| Cfr10I | RCCGGY | 1 cut(s) | 612 |
| Cfr13I | GGNCC | 1 cut(s) | 54 |
| Csp6I | GTAC | 2 cut(s) | 80, 610 |
| CspAI | ACCGGT | 1 cut(s) | 612 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 444, 479 |
| CviAII | CATG | 5 cut(s) | 125, 191, 370, 399, 607 |
| CviJI | RGCY | 7 cut(s) | 117, 137, 315, 403, 412, 600, 641 |
| CviKI_1 | RGCY | 7 cut(s) | 117, 137, 315, 403, 412, 600, 641 |
| CviQI | GTAC | 2 cut(s) | 80, 610 |
| DdeI | CTNAG | 6 cut(s) | 23, 288, 294, 342, 413, 623 |
| DinI | GGCGCC | 1 cut(s) | 29 |
| DpnI | GATC | 1 cut(s) | 214 |
| DpnII | GATC | 1 cut(s) | 212 |
| DriI | GACNNNNNGTC | 1 cut(s) | 297 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 297 |
| EciI | GGCGGA | 1 cut(s) | 157 |
| Ecl136II | GAGCTC | 1 cut(s) | 315 |
| Eco24I | GRGCYC | 1 cut(s) | 317 |
| Eco32I | GATATC | 1 cut(s) | 222 |
| Eco47I | GGWCC | 1 cut(s) | 54 |
| Eco53kI | GAGCTC | 1 cut(s) | 315 |
| EcoICRI | GAGCTC | 1 cut(s) | 315 |
| EcoRII | CCWGG | 1 cut(s) | 592 |
| EcoRV | GATATC | 1 cut(s) | 222 |
| EcoT38I | GRGCYC | 1 cut(s) | 317 |
| EgeI | GGCGCC | 1 cut(s) | 29 |
| EheI | GGCGCC | 1 cut(s) | 29 |
| FaeI | CATG | 5 cut(s) | 128, 194, 373, 402, 610 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 524, 556 |
| FaqI | GGGAC | 1 cut(s) | 575 |
| FatI | CATG | 5 cut(s) | 124, 190, 369, 398, 606 |
| Fnu4HI | GCNGC | 5 cut(s) | 171, 236, 564, 598, 660 |
| FokI | GGATG | 2 cut(s) | 81, 535 |
| FriOI | GRGCYC | 1 cut(s) | 317 |
| Fsp4HI | GCNGC | 5 cut(s) | 171, 236, 564, 598, 660 |
| FspBI | CTAG | 2 cut(s) | 197, 578 |
| GlaI | GCGC | 1 cut(s) | 29 |
| GluI | GCNGC | 5 cut(s) | 171, 236, 564, 598, 660 |
| GsuI | CTGGAG | 1 cut(s) | 298 |
| HaeII | RGCGCY | 1 cut(s) | 31 |
| HaeIII | GGCC | 1 cut(s) | 117 |
| HapII | CCGG | 1 cut(s) | 613 |
| HhaI | GCGC | 1 cut(s) | 30 |
| Hin1I | GRCGYC | 1 cut(s) | 28 |
| Hin1II | CATG | 5 cut(s) | 128, 194, 373, 402, 610 |
| Hin6I | GCGC | 1 cut(s) | 28 |
| HinP1I | GCGC | 1 cut(s) | 28 |
| HinfI | GANTC | 6 cut(s) | 100, 184, 270, 292, 298, 582 |
| HpaII | CCGG | 1 cut(s) | 613 |
| Hpy166II | GTNNAC | 2 cut(s) | 130, 149 |
| Hpy188I | TCNGA | 7 cut(s) | 68, 212, 219, 297, 498, 505, 630 |
| Hpy188III | TCNNGA | 4 cut(s) | 97, 249, 277, 287 |
| Hpy8I | GTNNAC | 2 cut(s) | 130, 149 |
| HpyAV | CCTTC | 2 cut(s) | 367, 405 |
| HpyCH4III | ACNGT | 2 cut(s) | 58, 84 |
| HpyCH4IV | ACGT | 1 cut(s) | 145 |
| HpyCH4V | TGCA | 2 cut(s) | 76, 392 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 17, 167, 409, 572 |
| HpyF3I | CTNAG | 6 cut(s) | 23, 288, 294, 342, 413, 623 |
| HpySE526I | ACGT | 1 cut(s) | 145 |
| Hsp92I | GRCGYC | 1 cut(s) | 28 |
| Hsp92II | CATG | 5 cut(s) | 128, 194, 373, 402, 610 |
| HspAI | GCGC | 1 cut(s) | 28 |
| KasI | GGCGCC | 1 cut(s) | 27 |
| Kzo9I | GATC | 1 cut(s) | 212 |
| LmnI | GCTCC | 1 cut(s) | 8 |
| Lsp1109I | GCAGC | 2 cut(s) | 550, 609 |
| LweI | GCATC | 2 cut(s) | 459, 513 |
| MaeI | CTAG | 2 cut(s) | 197, 578 |
| MaeII | ACGT | 1 cut(s) | 145 |
| MalI | GATC | 1 cut(s) | 214 |
| MbiI | CCGCTC | 1 cut(s) | 20 |
| MboI | GATC | 1 cut(s) | 212 |
| MboII | GAAGA | 4 cut(s) | 52, 146, 179, 646 |
| MflI | RGATCY | 1 cut(s) | 212 |
| MhlI | GDGCHC | 1 cut(s) | 317 |
| MluCI | AATT | 3 cut(s) | 110, 240, 471 |
| Mly113I | GGCGCC | 1 cut(s) | 28 |
| MlyI | GAGTC | 4 cut(s) | 94, 279, 286, 307 |
| MmeI | TCCRAC | 1 cut(s) | 467 |
| MnlI | CCTC | 5 cut(s) | 74, 284, 370, 612, 624 |
| MslI | CAYNNNNRTG | 2 cut(s) | 123, 397 |
| MspI | CCGG | 1 cut(s) | 613 |
| MspR9I | CCNGG | 1 cut(s) | 594 |
| MvaI | CCWGG | 1 cut(s) | 594 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 17, 167, 409, 572 |
| NarI | GGCGCC | 1 cut(s) | 28 |
| NdeII | GATC | 1 cut(s) | 212 |
| NlaIII | CATG | 5 cut(s) | 128, 194, 373, 402, 610 |
| NlaIV | GGNNCC | 1 cut(s) | 29 |
| NspI | RCATGY | 2 cut(s) | 128, 610 |
| PciI | ACATGT | 1 cut(s) | 606 |
| PfeI | GAWTC | 2 cut(s) | 184, 582 |
| PinAI | ACCGGT | 1 cut(s) | 612 |
| PkrI | GCNGC | 5 cut(s) | 172, 237, 565, 599, 661 |
| PleI | GAGTC | 4 cut(s) | 94, 278, 286, 306 |
| PluTI | GGCGCC | 1 cut(s) | 31 |
| PpsI | GAGTC | 4 cut(s) | 94, 278, 286, 306 |
| PscI | ACATGT | 1 cut(s) | 606 |
| Psp124BI | GAGCTC | 1 cut(s) | 317 |
| Psp6I | CCWGG | 1 cut(s) | 592 |
| PspGI | CCWGG | 1 cut(s) | 592 |
| PspN4I | GGNNCC | 1 cut(s) | 29 |
| PspPI | GGNCC | 1 cut(s) | 54 |
| PstNI | CAGNNNCTG | 1 cut(s) | 82 |
| PsuI | RGATCY | 1 cut(s) | 212 |
| RsaI | GTAC | 2 cut(s) | 81, 611 |
| RsaNI | GTAC | 2 cut(s) | 80, 610 |
| RseI | CAYNNNNRTG | 2 cut(s) | 123, 397 |
| SacI | GAGCTC | 1 cut(s) | 317 |
| SatI | GCNGC | 5 cut(s) | 171, 236, 564, 598, 660 |
| Sau3AI | GATC | 1 cut(s) | 212 |
| Sau96I | GGNCC | 1 cut(s) | 54 |
| SchI | GAGTC | 4 cut(s) | 94, 279, 286, 307 |
| ScrFI | CCNGG | 1 cut(s) | 594 |
| SduI | GDGCHC | 1 cut(s) | 317 |
| SetI | ASST | 7 cut(s) | 81, 148, 205, 317, 397, 419, 643 |
| SfaNI | GCATC | 2 cut(s) | 459, 513 |
| SfoI | GGCGCC | 1 cut(s) | 29 |
| SinI | GGWCC | 1 cut(s) | 54 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 123, 397 |
| SmlI | CTYRAG | 1 cut(s) | 95 |
| SmoI | CTYRAG | 1 cut(s) | 95 |
| Sse9I | AATT | 3 cut(s) | 110, 240, 471 |
| SsiI | CCGC | 6 cut(s) | 5, 18, 168, 171, 236, 659 |
| SspDI | GGCGCC | 1 cut(s) | 27 |
| SspMI | CTAG | 2 cut(s) | 197, 578 |
| SstI | GAGCTC | 1 cut(s) | 317 |
| StyD4I | CCNGG | 1 cut(s) | 592 |
| TaaI | ACNGT | 2 cut(s) | 58, 84 |
| TaiI | ACGT | 1 cut(s) | 148 |
| TaqI | TCGA | 2 cut(s) | 352, 549 |
| TasI | AATT | 3 cut(s) | 110, 240, 471 |
| TauI | GCSGC | 3 cut(s) | 173, 238, 662 |
| TfiI | GAWTC | 2 cut(s) | 184, 582 |
| TscAI | CASTG | 2 cut(s) | 237, 346 |
| TseI | GCWGC | 2 cut(s) | 563, 597 |
| TspDTI | ATGAA | 3 cut(s) | 179, 386, 387 |
| TspRI | CASTG | 2 cut(s) | 237, 346 |
| VpaK11BI | GGWCC | 1 cut(s) | 54 |
| XceI | RCATGY | 2 cut(s) | 128, 610 |
| XspI | CTAG | 2 cut(s) | 197, 578 |