Rh2CG228000

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0006547
Physical Location & Seq
Forward (+)
23075200 .. 23079045
3846 bp
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UTR
Exon/CDS
Intron
Rh2CG228000.1

Sequence Viewer

Length: 438 bp
ATGGCGGGATCTTTGTCTCTTCTTCTCAGACGGCTTTACCTTTCCCTCTACAACTGGACCCTCTTCATCGGATGGTCTCAGGTGCTGTATCTTGCTGTGAAGACACTTCGGGAATCGGGCCCCGGGCAGGTTTACAATGCCCTCGACCGCCCACTTCAGTTTGCTCAATCCGCCGCGTTTTTGGAGATTCTTCATGGCTTAGTCGGTTTGGTGAGATCTCCTATAACAGCCACACTGCCACAGATTAGTTCGAGGTTGTTTCTGGTTTGGGGGATCCTGTGGAGCTTTCCTGAGACTCAGAGTCATGTCCTTGTGAGCTCTTTGGTCATCAGCTGGTCCATCACTGAGATTATTCGATACTCTTTCTATGGCATGAAAGAGACTCTTGGTTTTGCGCCTTCCTGGCTCCAGTGGCTCAGGTACTATACACTGTTTTAG

Protein Analysis

145

Amino Acids

16.65

Weight (kDa)

9.63

Isoelectric Point (pI)

55.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTPLA PF04387 56 - 143 1.1e-30 Protein tyrosine phosphatase-like protein, PTPLA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 118
AccII CGCG 1 cut(s) 176
AciI CCGC 4 cut(s) 5, 148, 171, 174
AclWI GGATC 3 cut(s) 16, 268, 281
AcuI CTGAAG 1 cut(s) 140
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 1 cut(s) 127
AhdI GACNNNNNGTC 1 cut(s) 300
AjnI CCWGG 1 cut(s) 401
AluBI AGCT 3 cut(s) 285, 318, 333
AluI AGCT 3 cut(s) 285, 318, 333
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 4 cut(s) 21, 81, 287, 374
AlwI GGATC 3 cut(s) 16, 268, 281
AlwNI CAGNNNCTG 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 1 cut(s) 118
ApaI GGGCCC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 4 cut(s) 57, 118, 119, 336
AsuC2I CCSGG 2 cut(s) 123, 124
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 2 cut(s) 57, 336
BaeGI GKGCMC 1 cut(s) 122
BamHI GGATCC 1 cut(s) 273
BanII GRGCYC 2 cut(s) 122, 320
BbsI GAAGAC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 320
BccI CCATC 2 cut(s) 66, 347
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 403
BcnI CCSGG 2 cut(s) 123, 124
BcoDI GTCTC 4 cut(s) 21, 81, 287, 374
BfuAI ACCTGC 1 cut(s) 118
BglI GCCNNNNNGGC 1 cut(s) 403
BglII AGATCT 1 cut(s) 215
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 3 cut(s) 123, 124, 403
Bme18I GGWCC 2 cut(s) 57, 336
BmeRI GACNNNNNGTC 1 cut(s) 300
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 4 cut(s) 57, 118, 119, 336
BmiI GGNNCC 5 cut(s) 59, 120, 121, 275, 407
BmrFI CCNGG 3 cut(s) 123, 124, 403
BpiI GAAGAC 1 cut(s) 107
BpmI CTGGAG 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 416
BpuMI CCSGG 2 cut(s) 123, 124
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 121, 122
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse1I ACTGG 2 cut(s) 59, 409
BseBI CCWGG 1 cut(s) 403
BseDI CCNNGG 2 cut(s) 121, 122
BseGI GGATG 1 cut(s) 77
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 6 cut(s) 40, 92, 282, 311, 336, 430
BseNI ACTGG 2 cut(s) 59, 409
BseSI GKGCMC 1 cut(s) 122
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 127
BsmAI GTCTC 4 cut(s) 21, 81, 287, 374
BsnI GGCC 1 cut(s) 120
Bso31I GGTCTC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 122
Bsp120I GGGCCC 1 cut(s) 118
Bsp1286I GDGCHC 2 cut(s) 122, 320
Bsp143I GATC 3 cut(s) 8, 215, 273
BspACI CCGC 4 cut(s) 5, 148, 171, 174
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 6 cut(s) 39, 91, 283, 310, 337, 429
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 5 cut(s) 59, 120, 121, 275, 407
BspMI ACCTGC 1 cut(s) 118
BspPI GGATC 3 cut(s) 16, 268, 281
BspTNI GGTCTC 1 cut(s) 81
BsrI ACTGG 2 cut(s) 59, 409
BssECI CCNNGG 2 cut(s) 121, 122
BssMI GATC 3 cut(s) 8, 215, 273
Bst2UI CCWGG 1 cut(s) 403
Bst4CI ACNGT 1 cut(s) 432
Bst6I CTCTTC 2 cut(s) 24, 68
BstDEI CTNAG 7 cut(s) 26, 78, 199, 291, 297, 345, 416
BstF5I GGATG 1 cut(s) 77
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 3 cut(s) 11, 218, 276
BstMAI GTCTC 4 cut(s) 21, 81, 287, 374
BstMBI GATC 3 cut(s) 8, 215, 273
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 3 cut(s) 170, 403, 412
BstNI CCWGG 1 cut(s) 403
BstSCI CCNGG 3 cut(s) 121, 122, 401
BstSLI GKGCMC 1 cut(s) 122
BstUI CGCG 1 cut(s) 176
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 3 cut(s) 8, 215, 273
BstYI RGATCY 3 cut(s) 8, 215, 273
BsuRI GGCC 1 cut(s) 120
BtsCI GGATG 1 cut(s) 77
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 4 cut(s) 233, 342, 416, 428
BveI ACCTGC 1 cut(s) 118
CaiI CAGNNNCTG 1 cut(s) 85
CfoI GCGC 1 cut(s) 397
Cfr13I GGNCC 4 cut(s) 57, 118, 119, 336
Cfr9I CCCGGG 1 cut(s) 122
Csp6I GTAC 1 cut(s) 421
CviAII CATG 3 cut(s) 194, 305, 373
CviJI RGCY 9 cut(s) 34, 120, 198, 230, 285, 318, 333, 406, 415
CviKI_1 RGCY 9 cut(s) 34, 120, 198, 230, 285, 318, 333, 406, 415
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 7 cut(s) 26, 78, 199, 291, 297, 345, 416
DpnI GATC 3 cut(s) 10, 217, 275
DpnII GATC 3 cut(s) 8, 215, 273
DriI GACNNNNNGTC 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 24, 68
Eam1105I GACNNNNNGTC 1 cut(s) 300
EarI CTCTTC 2 cut(s) 24, 68
EciI GGCGGA 1 cut(s) 160
Ecl136II GAGCTC 1 cut(s) 318
Eco24I GRGCYC 2 cut(s) 122, 320
Eco31I GGTCTC 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 57, 336
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 122
EcoICRI GAGCTC 1 cut(s) 318
EcoO109I RGGNCCY 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 401
EcoT38I GRGCYC 2 cut(s) 122, 320
FaeI CATG 3 cut(s) 197, 308, 376
FaiI YATR 6 cut(s) 195, 224, 306, 369, 374, 426
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FatI CATG 3 cut(s) 193, 304, 372
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 84
FriOI GRGCYC 2 cut(s) 122, 320
Fsp4HI GCNGC 1 cut(s) 174
GlaI GCGC 1 cut(s) 396
GluI GCNGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 1 cut(s) 120
HapII CCGG 1 cut(s) 123
HhaI GCGC 1 cut(s) 397
Hin1II CATG 3 cut(s) 197, 308, 376
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HinfI GANTC 5 cut(s) 113, 187, 295, 301, 382
HpaII CCGG 1 cut(s) 123
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 3 cut(s) 29, 71, 300
Hpy188III TCNNGA 2 cut(s) 110, 290
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 1 cut(s) 408
HpyCH4III ACNGT 1 cut(s) 432
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 403, 412
HpyF3I CTNAG 7 cut(s) 26, 78, 199, 291, 297, 345, 416
Hsp92II CATG 3 cut(s) 197, 308, 376
HspAI GCGC 1 cut(s) 395
Kzo9I GATC 3 cut(s) 8, 215, 273
LmnI GCTCC 2 cut(s) 282, 411
MalI GATC 3 cut(s) 10, 217, 275
MboI GATC 3 cut(s) 8, 215, 273
MboII GAAGA 5 cut(s) 11, 14, 55, 112, 182
MflI RGATCY 3 cut(s) 8, 215, 273
MhlI GDGCHC 2 cut(s) 122, 320
MlyI GAGTC 3 cut(s) 289, 310, 376
MnlI CCTC 4 cut(s) 56, 71, 152, 246
MspA1I CMGCKG 1 cut(s) 333
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 3 cut(s) 123, 124, 403
MvaI CCWGG 1 cut(s) 403
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 170, 403, 412
NciI CCSGG 2 cut(s) 123, 124
NdeII GATC 3 cut(s) 8, 215, 273
NlaIII CATG 3 cut(s) 197, 308, 376
NlaIV GGNNCC 5 cut(s) 59, 120, 121, 275, 407
PfeI GAWTC 2 cut(s) 113, 187
PkrI GCNGC 1 cut(s) 175
PleI GAGTC 3 cut(s) 289, 309, 376
PpsI GAGTC 3 cut(s) 289, 309, 376
Psp124BI GAGCTC 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 401
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 5 cut(s) 59, 120, 121, 275, 407
PspOMI GGGCCC 1 cut(s) 118
PspPI GGNCC 4 cut(s) 57, 118, 119, 336
PstNI CAGNNNCTG 1 cut(s) 85
PsuI RGATCY 3 cut(s) 8, 215, 273
PvuII CAGCTG 1 cut(s) 333
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SacI GAGCTC 1 cut(s) 320
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 3 cut(s) 8, 215, 273
Sau96I GGNCC 4 cut(s) 57, 118, 119, 336
SchI GAGTC 3 cut(s) 289, 310, 376
ScrFI CCNGG 3 cut(s) 123, 124, 403
SduI GDGCHC 2 cut(s) 122, 320
SetI ASST 8 cut(s) 42, 84, 132, 257, 287, 320, 335, 422
SinI GGWCC 2 cut(s) 57, 336
SmaI CCCGGG 1 cut(s) 124
SsiI CCGC 4 cut(s) 5, 148, 171, 174
SstI GAGCTC 1 cut(s) 320
StyD4I CCNGG 3 cut(s) 121, 122, 401
TaaI ACNGT 1 cut(s) 432
TaqI TCGA 3 cut(s) 144, 251, 355
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 2 cut(s) 113, 187
TscAI CASTG 4 cut(s) 240, 349, 416, 435
TspDTI ATGAA 3 cut(s) 55, 182, 389
TspMI CCCGGG 1 cut(s) 122
TspRI CASTG 4 cut(s) 240, 349, 416, 435
VpaK11BI GGWCC 2 cut(s) 57, 336
XmaI CCCGGG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.