Rorug02G0172000

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006547
Physical Location & Seq
Forward (+)
15639689 .. 15640247
559 bp
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UTR
Exon/CDS
Intron
Rorug02G0172000.1

Sequence Viewer

Length: 447 bp
ATGGCAATGACAGTTGTCGAGGTCGGAGAGGCTTCTCTGGTGCCAGCATTTCAACTTCTGTATGACAGCGTTAACCAAGCAATGGACAAAAAGACTGGCAACTTTAAGTACGTCCTCGTAAACCTCAAGTGCACTACTCTAGACCCTTTACAGAAACGGGTCCAGCGCGTGATCGGATATGAAGAGCGCAACGTGAAGTTGGGTCTCTCCAAGGAAGAAGTAGAAAACCTGATCACACTGATGCACAAGGGAGTAAAGCAGGTCAGAAAGTTTTCGACGTTGAAGTTTCGTATGAGGTACTACTGGTGCTGCATCAATGATCACGTTAGGGAAGTTAATGAGTTGGATACACATCTTCGAGAATTGTTGGAAATGCTGTGGCAACAGGAATTGGAGGATCTAAGGAAGGAGAGCACTACATACTTGGTGAAGAATAGAGGACTTTAG

Protein Analysis

148

Amino Acids

17.47

Weight (kDa)

8.73

Isoelectric Point (pI)

32.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 130 8.2e-06 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 250
AccB1I GGYRCC 1 cut(s) 40
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 1 cut(s) 168
AclWI GGATC 1 cut(s) 405
AfaI GTAC 2 cut(s) 110, 299
AfiI CCNNNNNNNGG 1 cut(s) 82
AgsI TTSAA 2 cut(s) 53, 283
Alw21I GWGCWC 2 cut(s) 134, 416
Alw26I GTCTC 1 cut(s) 209
Alw44I GTGCAC 1 cut(s) 130
AlwI GGATC 1 cut(s) 405
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 309
Asp700I GAANNNNTTC 1 cut(s) 271
AspLEI GCGC 2 cut(s) 168, 189
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 439
AvaII GGWCC 1 cut(s) 160
BaeGI GKGCMC 1 cut(s) 134
BanI GGYRCC 1 cut(s) 40
Bbv12I GWGCWC 2 cut(s) 134, 416
BbvI GCAGC 1 cut(s) 296
BciVI GTATCC 1 cut(s) 340
BclI TGATCA 2 cut(s) 231, 319
BcoDI GTCTC 1 cut(s) 209
BfaI CTAG 1 cut(s) 140
BfuAI ACCTGC 1 cut(s) 250
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 1 cut(s) 310
BlsI GCNGC 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 2 cut(s) 42, 161
BmsI GCATC 2 cut(s) 231, 321
BoxI GACNNNNGTC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 210
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 2 cut(s) 100, 308
Bse3DI GCAATG 2 cut(s) 12, 87
Bse8I GATNNNNATC 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 210
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 2 cut(s) 12, 87
BseNI ACTGG 2 cut(s) 100, 308
BseSI GKGCMC 1 cut(s) 134
BseXI GCAGC 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 168
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 2 cut(s) 134, 416
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 1 cut(s) 209
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 134, 416
Bsp143I GATC 4 cut(s) 171, 231, 319, 397
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 42, 161
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 1 cut(s) 405
BspQI GCTCTTC 1 cut(s) 177
BspT107I GGYRCC 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 12, 87
BsrI ACTGG 2 cut(s) 100, 308
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 4 cut(s) 171, 231, 319, 397
BssT1I CCWWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 13
Bst6I CTCTTC 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 1 cut(s) 401
BstFNI CGCG 1 cut(s) 168
BstHHI GCGC 2 cut(s) 168, 189
BstKTI GATC 4 cut(s) 174, 234, 322, 400
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 4 cut(s) 171, 231, 319, 397
BstPAI GACNNNNGTC 1 cut(s) 14
BstSLI GKGCMC 1 cut(s) 134
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BsuI GTATCC 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 236
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 45
CfoI GCGC 2 cut(s) 168, 189
Cfr13I GGNCC 1 cut(s) 160
Csp6I GTAC 2 cut(s) 109, 298
CviJI RGCY 1 cut(s) 32
CviKI_1 RGCY 1 cut(s) 32
CviQI GTAC 2 cut(s) 109, 298
DdeI CTNAG 1 cut(s) 401
DpnI GATC 4 cut(s) 173, 233, 321, 399
DpnII GATC 4 cut(s) 171, 231, 319, 397
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaiI YATR 4 cut(s) 63, 180, 293, 421
FbaI TGATCA 2 cut(s) 231, 319
Fnu4HI GCNGC 1 cut(s) 310
Fsp4HI GCNGC 1 cut(s) 310
FspBI CTAG 1 cut(s) 140
GlaI GCGC 2 cut(s) 167, 188
GluI GCNGC 1 cut(s) 310
HhaI GCGC 2 cut(s) 168, 189
Hin6I GCGC 2 cut(s) 166, 187
HinP1I GCGC 2 cut(s) 166, 187
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HpaI GTTAAC 1 cut(s) 73
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 3 cut(s) 73, 121, 132
Hpy188I TCNGA 3 cut(s) 26, 176, 266
Hpy188III TCNNGA 2 cut(s) 140, 359
Hpy8I GTNNAC 3 cut(s) 73, 121, 132
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 400
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 4 cut(s) 111, 192, 278, 324
HpyCH4V TGCA 3 cut(s) 132, 244, 312
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 4 cut(s) 111, 192, 278, 324
HspAI GCGC 2 cut(s) 166, 187
Ksp22I TGATCA 2 cut(s) 231, 319
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 4 cut(s) 171, 231, 319, 397
LguI GCTCTTC 1 cut(s) 177
LpnPI CCDG 8 cut(s) 23, 57, 81, 176, 242, 245, 289, 371
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 2 cut(s) 231, 321
MaeI CTAG 1 cut(s) 140
MaeII ACGT 4 cut(s) 111, 192, 278, 324
MalI GATC 4 cut(s) 173, 233, 321, 399
MboI GATC 4 cut(s) 171, 231, 319, 397
MboII GAAGA 4 cut(s) 194, 227, 347, 442
MflI RGATCY 1 cut(s) 397
MhlI GDGCHC 2 cut(s) 134, 416
MluCI AATT 2 cut(s) 362, 389
MmeI TCCRAC 3 cut(s) 4, 324, 348
MnlI CCTC 7 cut(s) 13, 22, 125, 134, 288, 388, 431
MroXI GAANNNNTTC 1 cut(s) 271
MseI TTAA 3 cut(s) 72, 105, 336
MslI CAYNNNNRTG 1 cut(s) 239
MvnI CGCG 1 cut(s) 168
NdeII GATC 4 cut(s) 171, 231, 319, 397
NlaIV GGNNCC 2 cut(s) 42, 161
PciSI GCTCTTC 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 1 cut(s) 311
PshAI GACNNNNGTC 1 cut(s) 14
PspN4I GGNNCC 2 cut(s) 42, 161
PspPI GGNCC 1 cut(s) 160
PsuI RGATCY 1 cut(s) 397
RsaI GTAC 2 cut(s) 110, 299
RsaNI GTAC 2 cut(s) 109, 298
RseI CAYNNNNRTG 1 cut(s) 239
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 3 cut(s) 72, 105, 336
SatI GCNGC 1 cut(s) 310
Sau3AI GATC 4 cut(s) 171, 231, 319, 397
Sau96I GGNCC 1 cut(s) 160
SduI GDGCHC 2 cut(s) 134, 416
SetI ASST 9 cut(s) 24, 114, 126, 195, 231, 264, 281, 299, 327
SfaNI GCATC 2 cut(s) 231, 321
SinI GGWCC 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 2 cut(s) 362, 389
SspMI CTAG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 4 cut(s) 114, 195, 281, 327
TaqI TCGA 3 cut(s) 18, 275, 358
TasI AATT 2 cut(s) 362, 389
Tru1I TTAA 3 cut(s) 72, 105, 336
Tru9I TTAA 3 cut(s) 72, 105, 336
TscAI CASTG 1 cut(s) 243
TseI GCWGC 1 cut(s) 309
TspDTI ATGAA 1 cut(s) 195
TspRI CASTG 1 cut(s) 243
Van91I CCANNNNNTGG 1 cut(s) 82
VneI GTGCAC 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 160
XbaI TCTAGA 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 271
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.