FvH4_1g23350

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011515
Physical Location & Seq
Reverse (-)
15201831 .. 15204170
2340 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23350.t1

Sequence Viewer

Length: 1164 bp
ATGTGTTTTTTAAAGGTGGCGAATCCCAGCAGAGACAGAGACGACGACCGCGCCGCCGCGGCGGGGCAAGGGAATGAGACACCACAAACGGAAGAACAGAGAGACCTTATTGAAGAGTGGCTGCTTTTCGAGCCTCTGCTCTTTTCTCAGCAACAACGACGTGGCAGTGGTGGAGGAGGAGGGATAATGGCGTCGTCGTCAATGCTGTTGAGGTTGGATAGGGAGAGGGAGATGTCGGCTATGGTTTCGGCTCTGACGCATGTTGTGACCGGAGAGGAGCAGGCTCATGAGGTTGATGATTTGAATTCGTCGCCGGGCGGTGTTGGGAGCAAGAGAGGAAGAGAAGACGACGGTGGTGGTGGTGGTGGTGGTGGAGAGCACGTGACAGAGTCGGTTTCAAGGCTTTGCAGAGCATTTGGTGATTTTCCACAGCAGGGTTCATCTTCCTCGTCCTCTGTTTTACCAGAAAACTCGATCACAAGAAATAGGAGTACTAATGTCACACCAGGTATACAAACAGCATATGAATATAGGGCACCTCAGATATCAGAGCAACCAACAAGAAGAAAATACAGAGGAGTGAGGCAGAGGCCATGGGGGAAATGGGCAGCAGAGATCAGAGACCCGTTCAAGGCTGCCAGAGTTTGGTTAGGCACATTCGACACAGCAGAAGCCGCCGCGCAAGCCTATGATACAGCTGCCTTGCGCTTCCGAGGCAACAAGGCCAAACTCAACTTCCCGGAAAACGTTAGACTCCGTTCACTACCCTCATCAGCTGCAGAAAATAATCCAATGACGAGTCAACCTACTGTTTCGGGTTCTCCGAATGCCCTTCTCTCCATTCCTACATCCACCGAGGCTATTGTTCACTCCCATGCAGCTACTTCATTCCAACAACAAAGGGCGCCTATGAATCTTTATGATCAGATGGTTTTGTTGTCTTCGTCTTCATCTCCATCAGCTACTACTTCTTCTTCTACACTTGCCTCCTCTGTTTCCTCTACCTCTTCTTCGCCGCCACCACATGTCTTCTCATCACGTTTTATTGCTCAGTCAACTTCACCGGTTCAGCTCAGACAAGCCCCCGGTGATCGTAGTGGCGGAGCTGATTCGTCAATGCAGCATCAGTGGTCTACGAGCTCCGGTCACCACAGTACTTCCTAG

Protein Analysis

388

Amino Acids

41.5

Weight (kDa)

6.77

Isoelectric Point (pI)

60.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 190 - 239 1.2e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 535, 904
AccB7I CCANNNNNTGG 1 cut(s) 645
AccI GTMKAC 2 cut(s) 511, 1133
AccII CGCG 3 cut(s) 51, 59, 680
AclI AACGTT 1 cut(s) 747
AcsI RAATTY 1 cut(s) 304
AcvI CACGTG 1 cut(s) 382
AcyI GRCGYC 2 cut(s) 191, 905
AfaI GTAC 2 cut(s) 493, 1156
AfiI CCNNNNNNNGG 4 cut(s) 63, 434, 631, 645
AflIII ACRYGT 1 cut(s) 1024
AgeI ACCGGT 1 cut(s) 1063
AgsI TTSAA 4 cut(s) 113, 304, 399, 631
AjiI CACGTC 1 cut(s) 161
AjnI CCWGG 1 cut(s) 505
AjuI GAANNNNNNNTTGG 2 cut(s) 719, 751
AluBI AGCT 7 cut(s) 698, 776, 881, 962, 1072, 1106, 1140
AluI AGCT 7 cut(s) 698, 776, 881, 962, 1072, 1106, 1140
Alw21I GWGCWC 2 cut(s) 381, 1142
Alw26I GTCTC 5 cut(s) 27, 33, 71, 96, 615
AoxI GGCC 2 cut(s) 590, 723
ApeKI GCWGC 7 cut(s) 121, 608, 635, 698, 776, 878, 1120
ApoI RAATTY 1 cut(s) 304
AsiGI ACCGGT 1 cut(s) 1063
AspLEI GCGC 4 cut(s) 53, 682, 708, 907
AsuC2I CCSGG 3 cut(s) 315, 740, 1086
AsuHPI GGTGA 4 cut(s) 431, 1053, 1100, 1139
BaeGI GKGCMC 1 cut(s) 538
BanI GGYRCC 2 cut(s) 535, 904
BanII GRGCYC 1 cut(s) 1142
BbrPI CACGTG 1 cut(s) 382
BbsI GAAGAC 4 cut(s) 351, 933, 939, 1021
Bbv12I GWGCWC 2 cut(s) 381, 1142
BbvI GCAGC 7 cut(s) 108, 620, 622, 685, 763, 890, 1132
BccI CCATC 2 cut(s) 922, 964
BciT130I CCWGG 1 cut(s) 507
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 3 cut(s) 315, 740, 1086
BcoDI GTCTC 5 cut(s) 27, 33, 71, 96, 615
BfaI CTAG 1 cut(s) 1162
BfmI CTRYAG 1 cut(s) 777
BfoI RGCGCY 1 cut(s) 908
BglI GCCNNNNNGGC 1 cut(s) 59
BmcAI AGTACT 2 cut(s) 493, 1156
Bme1390I CCNGG 4 cut(s) 315, 507, 740, 1086
BmgBI CACGTC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 537, 906
BmrFI CCNGG 4 cut(s) 315, 507, 740, 1086
BmsI GCATC 1 cut(s) 1132
BpiI GAAGAC 4 cut(s) 351, 933, 939, 1021
BpuMI CCSGG 3 cut(s) 315, 740, 1086
BsaAI YACGTR 1 cut(s) 382
BsaHI GRCGYC 2 cut(s) 191, 905
BsaI GGTCTC 2 cut(s) 96, 615
BsaJI CCNNGG 5 cut(s) 57, 593, 712, 855, 1084
BsaWI WCCGGW 3 cut(s) 269, 1063, 1142
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 434, 631, 645
Bse118I RCCGGY 1 cut(s) 1063
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 5 cut(s) 57, 593, 712, 855, 1084
BseGI GGATG 1 cut(s) 848
BseLI CCNNNNNNNGG 4 cut(s) 63, 434, 631, 645
BseMII CTCAG 4 cut(s) 161, 554, 1064, 1087
BseRI GAGGAG 5 cut(s) 189, 192, 290, 591, 979
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 7 cut(s) 108, 620, 622, 685, 763, 890, 1132
BseYI CCCAGC 1 cut(s) 26
Bsh1236I CGCG 3 cut(s) 51, 59, 680
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 2 cut(s) 592, 725
BshNI GGYRCC 2 cut(s) 535, 904
BshTI ACCGGT 1 cut(s) 1063
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 2 cut(s) 381, 1142
BsiSI CCGG 6 cut(s) 270, 314, 740, 1064, 1086, 1143
BslI CCNNNNNNNGG 4 cut(s) 63, 434, 631, 645
BsmAI GTCTC 5 cut(s) 27, 33, 71, 96, 615
BsmBI CGTCTC 1 cut(s) 33
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 2 cut(s) 592, 725
Bso31I GGTCTC 2 cut(s) 96, 615
Bsp1286I GDGCHC 3 cut(s) 381, 538, 1142
Bsp143I GATC 4 cut(s) 474, 615, 922, 1090
Bsp19I CCATGG 1 cut(s) 593
BspANI GGCC 2 cut(s) 592, 725
BspCNI CTCAG 4 cut(s) 160, 553, 1063, 1086
BspFNI CGCG 3 cut(s) 51, 59, 680
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 537, 906
BspMAI CTGCAG 1 cut(s) 781
BspT107I GGYRCC 2 cut(s) 535, 904
BspTNI GGTCTC 2 cut(s) 96, 615
BsrFI RCCGGY 1 cut(s) 1063
BssAI RCCGGY 1 cut(s) 1063
BssECI CCNNGG 5 cut(s) 57, 593, 712, 855, 1084
BssMI GATC 4 cut(s) 474, 615, 922, 1090
BssNAI GTATAC 1 cut(s) 512
BssNI GRCGYC 2 cut(s) 191, 905
BssT1I CCWWGG 1 cut(s) 593
Bst1107I GTATAC 1 cut(s) 512
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 3 cut(s) 353, 811, 1154
Bst6I CTCTTC 3 cut(s) 108, 334, 1012
BstACI GRCGYC 2 cut(s) 191, 905
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 282, 684
BstDEI CTNAG 4 cut(s) 147, 540, 1050, 1073
BstDSI CCRYGG 2 cut(s) 57, 593
BstEII GGTNACC 1 cut(s) 1145
BstF5I GGATG 1 cut(s) 848
BstFNI CGCG 3 cut(s) 51, 59, 680
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 4 cut(s) 53, 682, 708, 907
BstKTI GATC 4 cut(s) 477, 618, 925, 1093
BstMAI GTCTC 5 cut(s) 27, 33, 71, 96, 615
BstMBI GATC 4 cut(s) 474, 615, 922, 1090
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 5 cut(s) 59, 130, 674, 683, 714
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 2 cut(s) 263, 1028
BstPI GGTNACC 1 cut(s) 1145
BstSCI CCNGG 4 cut(s) 313, 505, 738, 1084
BstSFI CTRYAG 1 cut(s) 777
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 3 cut(s) 51, 59, 680
BstV1I GCAGC 7 cut(s) 108, 620, 622, 685, 763, 890, 1132
BstV2I GAAGAC 4 cut(s) 351, 933, 939, 1021
BstZ17I GTATAC 1 cut(s) 512
BsuRI GGCC 2 cut(s) 592, 725
BtgI CCRYGG 2 cut(s) 57, 593
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 1 cut(s) 848
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 2 cut(s) 172, 1133
Cac8I GCNNGC 2 cut(s) 282, 684
CciI TCATGA 1 cut(s) 286
CfoI GCGC 4 cut(s) 53, 682, 708, 907
Cfr10I RCCGGY 1 cut(s) 1063
Cfr42I CCGCGG 1 cut(s) 60
CseI GACGC 2 cut(s) 180, 265
CsiI ACCWGGT 1 cut(s) 505
Csp6I GTAC 2 cut(s) 492, 1155
CspAI ACCGGT 1 cut(s) 1063
CviAII CATG 5 cut(s) 260, 287, 594, 875, 1025
CviQI GTAC 2 cut(s) 492, 1155
DdeI CTNAG 4 cut(s) 147, 540, 1050, 1073
DinI GGCGCC 1 cut(s) 906
DpnI GATC 4 cut(s) 476, 617, 924, 1092
DpnII GATC 4 cut(s) 474, 615, 922, 1090
DraI TTTAAA 1 cut(s) 12
Eam1104I CTCTTC 3 cut(s) 108, 334, 1012
EarI CTCTTC 3 cut(s) 108, 334, 1012
EciI GGCGGA 1 cut(s) 1116
Ecl136II GAGCTC 1 cut(s) 1140
Eco130I CCWWGG 1 cut(s) 593
Eco24I GRGCYC 1 cut(s) 1142
Eco31I GGTCTC 2 cut(s) 96, 615
Eco32I GATATC 1 cut(s) 546
Eco53kI GAGCTC 1 cut(s) 1140
Eco72I CACGTG 1 cut(s) 382
Eco91I GGTNACC 1 cut(s) 1145
EcoICRI GAGCTC 1 cut(s) 1140
EcoO65I GGTNACC 1 cut(s) 1145
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 1 cut(s) 505
EcoRV GATATC 1 cut(s) 546
EcoT14I CCWWGG 1 cut(s) 593
EcoT38I GRGCYC 1 cut(s) 1142
EgeI GGCGCC 1 cut(s) 906
EheI GGCGCC 1 cut(s) 906
ErhI CCWWGG 1 cut(s) 593
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 5 cut(s) 263, 290, 597, 878, 1028
FatI CATG 5 cut(s) 259, 286, 593, 874, 1024
FauI CCCGC 1 cut(s) 55
FauNDI CATATG 1 cut(s) 523
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 2 cut(s) 511, 1133
FokI GGATG 1 cut(s) 835
FriOI GRGCYC 1 cut(s) 1142
FspBI CTAG 1 cut(s) 1162
GlaI GCGC 4 cut(s) 52, 681, 707, 906
GsaI CCCAGC 1 cut(s) 30
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 2 cut(s) 592, 725
HapII CCGG 6 cut(s) 270, 314, 740, 1064, 1086, 1143
HgaI GACGC 2 cut(s) 180, 265
HhaI GCGC 4 cut(s) 53, 682, 708, 907
Hin1I GRCGYC 2 cut(s) 191, 905
Hin1II CATG 5 cut(s) 263, 290, 597, 878, 1028
Hin6I GCGC 4 cut(s) 51, 680, 706, 905
HinP1I GCGC 4 cut(s) 51, 680, 706, 905
HincII GTYRAC 2 cut(s) 803, 1056
HindII GTYRAC 2 cut(s) 803, 1056
HinfI GANTC 6 cut(s) 22, 389, 753, 799, 913, 1109
HpaII CCGG 6 cut(s) 270, 314, 740, 1064, 1086, 1143
HphI GGTGA 4 cut(s) 431, 1053, 1100, 1139
Hpy166II GTNNAC 6 cut(s) 512, 761, 803, 868, 1056, 1134
Hpy188I TCNGA 8 cut(s) 255, 543, 550, 620, 713, 825, 927, 1076
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 6 cut(s) 512, 761, 803, 868, 1056, 1134
Hpy99I CGWCG 6 cut(s) 47, 162, 196, 199, 313, 353
HpyAV CCTTC 1 cut(s) 842
HpyCH4III ACNGT 3 cut(s) 353, 811, 1154
HpyCH4IV ACGT 4 cut(s) 160, 381, 747, 1039
HpyCH4V TGCA 4 cut(s) 408, 779, 878, 1120
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 130, 674, 683, 714
HpyF3I CTNAG 4 cut(s) 147, 540, 1050, 1073
HpySE526I ACGT 4 cut(s) 160, 381, 747, 1039
Hsp92I GRCGYC 2 cut(s) 191, 905
Hsp92II CATG 5 cut(s) 263, 290, 597, 878, 1028
HspAI GCGC 4 cut(s) 51, 680, 706, 905
KasI GGCGCC 1 cut(s) 904
Ksp22I TGATCA 1 cut(s) 922
KspI CCGCGG 1 cut(s) 60
Kzo9I GATC 4 cut(s) 474, 615, 922, 1090
LmnI GCTCC 4 cut(s) 277, 327, 1103, 1145
Lsp1109I GCAGC 7 cut(s) 108, 620, 622, 685, 763, 890, 1132
LweI GCATC 1 cut(s) 1132
MabI ACCWGGT 1 cut(s) 505
MaeI CTAG 1 cut(s) 1162
MaeII ACGT 4 cut(s) 160, 381, 747, 1039
MaeIII GTNAC 4 cut(s) 265, 382, 499, 1145
MalI GATC 4 cut(s) 476, 617, 924, 1092
MboI GATC 4 cut(s) 474, 615, 922, 1090
MhlI GDGCHC 3 cut(s) 381, 538, 1142
MluCI AATT 1 cut(s) 304
Mly113I GGCGCC 1 cut(s) 905
MlyI GAGTC 3 cut(s) 398, 747, 808
MmeI TCCRAC 2 cut(s) 195, 916
MseI TTAA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 873
MspA1I CMGCKG 3 cut(s) 59, 698, 776
MspI CCGG 6 cut(s) 270, 314, 740, 1064, 1086, 1143
MspR9I CCNGG 4 cut(s) 315, 507, 740, 1086
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 3 cut(s) 51, 59, 680
MwoI GCNNNNNNNGC 5 cut(s) 59, 130, 674, 683, 714
NarI GGCGCC 1 cut(s) 905
NciI CCSGG 3 cut(s) 315, 740, 1086
NcoI CCATGG 1 cut(s) 593
NdeI CATATG 1 cut(s) 523
NdeII GATC 4 cut(s) 474, 615, 922, 1090
NlaIII CATG 5 cut(s) 263, 290, 597, 878, 1028
NlaIV GGNNCC 2 cut(s) 537, 906
NmuCI GTSAC 4 cut(s) 265, 382, 499, 1145
NspI RCATGY 2 cut(s) 263, 1028
PagI TCATGA 1 cut(s) 286
PciI ACATGT 1 cut(s) 1024
PcsI WCGNNNNNNNCGW 1 cut(s) 821
PctI GAATGC 1 cut(s) 832
PfeI GAWTC 3 cut(s) 22, 913, 1109
PflFI GACNNNGTC 1 cut(s) 388
PflMI CCANNNNNTGG 1 cut(s) 645
PfoI TCCNGGA 1 cut(s) 738
PinAI ACCGGT 1 cut(s) 1063
PleI GAGTC 3 cut(s) 397, 747, 807
PluTI GGCGCC 1 cut(s) 908
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 3 cut(s) 397, 747, 807
Ppu21I YACGTR 1 cut(s) 382
PscI ACATGT 1 cut(s) 1024
Psp124BI GAGCTC 1 cut(s) 1142
Psp1406I AACGTT 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 505
PspCI CACGTG 1 cut(s) 382
PspEI GGTNACC 1 cut(s) 1145
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 2 cut(s) 537, 906
PstI CTGCAG 1 cut(s) 781
PsyI GACNNNGTC 1 cut(s) 388
PvuII CAGCTG 2 cut(s) 698, 776
RsaI GTAC 2 cut(s) 493, 1156
RsaNI GTAC 2 cut(s) 492, 1155
RseI CAYNNNNRTG 1 cut(s) 873
SacI GAGCTC 1 cut(s) 1142
SacII CCGCGG 1 cut(s) 60
SaqAI TTAA 1 cut(s) 11
Sau3AI GATC 4 cut(s) 474, 615, 922, 1090
ScaI AGTACT 2 cut(s) 493, 1156
SchI GAGTC 3 cut(s) 398, 747, 808
ScrFI CCNGG 4 cut(s) 315, 507, 740, 1086
SduI GDGCHC 3 cut(s) 381, 538, 1142
SexAI ACCWGGT 1 cut(s) 505
SfaNI GCATC 1 cut(s) 1132
SfcI CTRYAG 1 cut(s) 777
SfoI GGCGCC 1 cut(s) 906
Sfr303I CCGCGG 1 cut(s) 60
SgrBI CCGCGG 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 873
Sse9I AATT 1 cut(s) 304
SspDI GGCGCC 1 cut(s) 904
SspMI CTAG 1 cut(s) 1162
SstI GAGCTC 1 cut(s) 1142
StyD4I CCNGG 4 cut(s) 313, 505, 738, 1084
StyI CCWWGG 1 cut(s) 593
TaaI ACNGT 3 cut(s) 353, 811, 1154
TaiI ACGT 4 cut(s) 163, 384, 750, 1042
TaqI TCGA 3 cut(s) 129, 473, 660
TasI AATT 1 cut(s) 304
TatI WGTACW 2 cut(s) 491, 1154
TauI GCSGC 6 cut(s) 56, 59, 62, 677, 680, 1018
TfiI GAWTC 3 cut(s) 22, 913, 1109
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 2 cut(s) 172, 1133
TseFI GTSAC 4 cut(s) 265, 382, 499, 1145
TseI GCWGC 7 cut(s) 121, 608, 635, 698, 776, 878, 1120
Tsp45I GTSAC 4 cut(s) 265, 382, 499, 1145
TspDTI ATGAA 5 cut(s) 429, 540, 876, 926, 939
TspGWI ACGGA 2 cut(s) 104, 746
TspRI CASTG 2 cut(s) 172, 1133
Tth111I GACNNNGTC 1 cut(s) 388
Van91I CCANNNNNTGG 1 cut(s) 645
XapI RAATTY 1 cut(s) 304
XceI RCATGY 2 cut(s) 263, 1028
XcmI CCANNNNNNNNNTGG 1 cut(s) 600
XmiI GTMKAC 2 cut(s) 511, 1133
XspI CTAG 1 cut(s) 1162
ZrmI AGTACT 2 cut(s) 493, 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.