pycom15g29040

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011515
Physical Location & Seq
Reverse (-)
25663355 .. 25665089
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g29040.1

Sequence Viewer

Length: 1197 bp
ATGTGTGTATTAAAGGTGGCGAATTCAAGAGGTAATAGCGACCAACGTGCCGCAAGGCGCACCAACATTGAGGAGGGACAAGGAGGACATCATAACATTGGAGAATGGCTCAATGAACCCCTGCTTATTTCACATCAGCAACGACAGCGGGCTGGCGGTGGTGGAAGTGGGATATTAGCGTCGGCCGCATCGTCAATGCTGTTGCAATTGGATTGGGAGAGAGAGATGTCGGCGATGGTATCGGCCTTGACGCATGTGGTGGCCGGAGACGAAAATGAGGTGGCTGCTGCAGGTGTTAATTCTGATTCAAGGACTAGTTCATGGGGCAGTGTTGGAACTAAAAGAGGAAGAGAAGAAGAGGAAGAGGAAGAAGGCGGCGGCGGCGGCGGCGGTCCGTTGTCCGAGTCGGTTACAAGGCTTTGTAGAGCTTTCGATGAATTTCCACAGCACATTCAGGGGCCATCCTCTGCTTTTGGAGAAGGCTTAAGCACCAGGACAACCACAACAGCAATCCCTCCAACTCCATCTCCAACTCCAAGTAGTAGATATGTATACAGTGCGCCCGAAATAGCAGAGCCAATAAGGAGAAGATACAGAGGAGTGAGACAAAGACCGTGGGGAAAATGGGCTGCTGAGATTAGAGACCCCTTCAAGGCTGCCAGGGTGTGGTTAGGCACCTTTGAAACAGCCGAAGCCGCCGCCCAAGCCTACGATCAAGCTGCCCTCCGATTCAGAGGCAACAAGGCCAAACTCAACTTCCCTGAAAACGTCAGACTCCTGTCGTCAACCGCCGCTCCTCCGCCGACCCAGTTGAGCAATTCTGCTCCTCAATATACCCTTTTGTCCGTTGCCACATCCTGTGAGCCGATTGTTCACTCTCAAGCATTCCATTCGTCGGACGAGGTGCCTTCAACCTCAAGAAATCTTGTTAGCTATTCTGAGTTGGTTGATATGCAGAGGCAACCTATGAGCCTTTATGACCAGATGGTTCTATCATCCTCTGCTGTTGCTGAGGCTGCTGCTTCTTCGTCTTCCTCTTCTTCTTCGTTATCACCACCTCCGTTGTCTTCACTCTTTGGGGCTCGGTCATGGCTTCCCGTCGGACCATCCGTAAGTGACAACAGTACTAGTACTGTTGATTTTCCAATGCAGCAATGGTCTAGCTATGCTCAATATACTACTCCCTCGTCGAGATGA

Protein Analysis

399

Amino Acids

42.71

Weight (kDa)

5.88

Isoelectric Point (pI)

62.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 197 - 246 1.6e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 281
AccB1I GGYRCC 2 cut(s) 674, 904
AccB7I CCANNNNNTGG 1 cut(s) 666
AccBSI CCGCTC 1 cut(s) 794
AccI GTMKAC 1 cut(s) 552
AcoI YGGCCR 2 cut(s) 183, 261
AcsI RAATTY 2 cut(s) 22, 437
AfaI GTAC 2 cut(s) 1126, 1132
AfiI CCNNNNNNNGG 3 cut(s) 652, 666, 895
AflII CTTAAG 1 cut(s) 484
AgsI TTSAA 5 cut(s) 27, 309, 652, 683, 912
AhlI ACTAGT 2 cut(s) 314, 1127
AjnI CCWGG 2 cut(s) 491, 659
AjuI GAANNNNNNNTTGG 2 cut(s) 740, 772
AluBI AGCT 4 cut(s) 428, 719, 933, 1164
AluI AGCT 4 cut(s) 428, 719, 933, 1164
Alw26I GTCTC 3 cut(s) 261, 598, 636
AoxI GGCC 5 cut(s) 183, 243, 261, 458, 744
ApeKI GCWGC 8 cut(s) 284, 287, 629, 656, 719, 1016, 1019, 1150
ApoI RAATTY 2 cut(s) 22, 437
AspLEI GCGC 2 cut(s) 60, 562
AspS9I GGNCC 3 cut(s) 392, 458, 1103
AsuHPI GGTGA 1 cut(s) 1044
AvaII GGWCC 2 cut(s) 392, 1103
BanI GGYRCC 2 cut(s) 674, 904
BanII GRGCYC 1 cut(s) 1084
BbsI GAAGAC 2 cut(s) 1023, 1059
BbvCI CCTCAGC 1 cut(s) 1011
BbvI GCAGC 8 cut(s) 271, 274, 616, 643, 706, 1003, 1006, 1162
BccI CCATC 5 cut(s) 229, 469, 532, 979, 1114
BcgI CGANNNNNNTGC 6 cut(s) 29, 63, 701, 735, 873, 907
BciT130I CCWGG 2 cut(s) 493, 661
BcoDI GTCTC 3 cut(s) 261, 598, 636
BcuI ACTAGT 2 cut(s) 314, 1127
BfaI CTAG 3 cut(s) 315, 1128, 1161
BfmI CTRYAG 1 cut(s) 288
BfrI CTTAAG 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 281
BmcAI AGTACT 2 cut(s) 1126, 1132
Bme1390I CCNGG 2 cut(s) 493, 661
Bme18I GGWCC 2 cut(s) 392, 1103
BmgT120I GGNCC 3 cut(s) 392, 458, 1103
BmiI GGNNCC 3 cut(s) 459, 676, 906
BmrFI CCNGG 2 cut(s) 493, 661
BmrI ACTGGG 1 cut(s) 802
BmsI GCATC 1 cut(s) 197
BmuI ACTGGG 1 cut(s) 802
BoxI GACNNNNGTC 1 cut(s) 778
BpiI GAAGAC 2 cut(s) 1023, 1059
BplI GAGNNNNNCTC 2 cut(s) 93, 125
Bpu10I CCTNAGC 1 cut(s) 1011
BpuEI CTTGAG 2 cut(s) 864, 901
BsaI GGTCTC 1 cut(s) 636
BsaJI CCNNGG 2 cut(s) 614, 660
BsaXI ACNNNNNCTCC 4 cut(s) 511, 541, 778, 808
Bsc4I CCNNNNNNNGG 3 cut(s) 652, 666, 895
Bse1I ACTGG 1 cut(s) 808
Bse3DI GCAATG 1 cut(s) 1160
BseBI CCWGG 2 cut(s) 493, 661
BseDI CCNNGG 2 cut(s) 614, 660
BseGI GGATG 4 cut(s) 461, 854, 995, 1106
BseLI CCNNNNNNNGG 3 cut(s) 652, 666, 895
BseMI GCAATG 1 cut(s) 1160
BseMII CTCAG 3 cut(s) 624, 930, 1002
BseNI ACTGG 1 cut(s) 808
BseRI GAGGAG 4 cut(s) 86, 612, 786, 816
BseX3I CGGCCG 1 cut(s) 183
BseXI GCAGC 8 cut(s) 271, 274, 616, 643, 706, 1003, 1006, 1162
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 5 cut(s) 185, 245, 263, 460, 746
BshNI GGYRCC 2 cut(s) 674, 904
BsiEI CGRYCG 1 cut(s) 186
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 3 cut(s) 652, 666, 895
BsmAI GTCTC 3 cut(s) 261, 598, 636
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 884
BsnI GGCC 5 cut(s) 185, 245, 263, 460, 746
Bso31I GGTCTC 1 cut(s) 636
Bsp1286I GDGCHC 1 cut(s) 1084
Bsp143I GATC 1 cut(s) 712
BspANI GGCC 5 cut(s) 185, 245, 263, 460, 746
BspCNI CTCAG 3 cut(s) 625, 931, 1003
BspLI GGNNCC 3 cut(s) 459, 676, 906
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 1 cut(s) 281
BspT107I GGYRCC 2 cut(s) 674, 904
BspTI CTTAAG 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 636
BsrBI CCGCTC 1 cut(s) 794
BsrDI GCAATG 1 cut(s) 1160
BsrI ACTGG 1 cut(s) 808
BssECI CCNNGG 2 cut(s) 614, 660
BssMI GATC 1 cut(s) 712
BssNAI GTATAC 1 cut(s) 553
Bst1107I GTATAC 1 cut(s) 553
Bst2UI CCWGG 2 cut(s) 493, 661
Bst4CI ACNGT 4 cut(s) 557, 615, 1124, 1135
Bst6I CTCTTC 4 cut(s) 343, 351, 357, 1042
BstAFI CTTAAG 1 cut(s) 484
BstC8I GCNNGC 2 cut(s) 150, 154
BstDEI CTNAG 3 cut(s) 633, 939, 1011
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 4 cut(s) 461, 854, 995, 1106
BstHHI GCGC 2 cut(s) 60, 562
BstKTI GATC 1 cut(s) 715
BstMAI GTCTC 3 cut(s) 261, 598, 636
BstMBI GATC 1 cut(s) 712
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 8 cut(s) 145, 185, 381, 384, 387, 695, 704, 1016
BstNI CCWGG 2 cut(s) 493, 661
BstNSI RCATGY 1 cut(s) 257
BstPAI GACNNNNGTC 1 cut(s) 778
BstSCI CCNGG 2 cut(s) 491, 659
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 8 cut(s) 271, 274, 616, 643, 706, 1003, 1006, 1162
BstV2I GAAGAC 2 cut(s) 1023, 1059
BstZ17I GTATAC 1 cut(s) 553
BstZI CGGCCG 1 cut(s) 183
BsuRI GGCC 5 cut(s) 185, 245, 263, 460, 746
BtgI CCRYGG 1 cut(s) 614
BtgZI GCGATG 1 cut(s) 248
BtsCI GGATG 4 cut(s) 461, 854, 995, 1106
BtsI GCAGTG 1 cut(s) 334
BtsIMutI CAGTG 2 cut(s) 334, 562
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 2 cut(s) 150, 154
CfoI GCGC 2 cut(s) 60, 562
Cfr13I GGNCC 3 cut(s) 392, 458, 1103
CpoI CGGWCCG 1 cut(s) 392
CseI GACGC 2 cut(s) 168, 259
Csp6I GTAC 2 cut(s) 1125, 1131
CspCI CAANNNNNGTGG 4 cut(s) 596, 631, 1044, 1079
CspI CGGWCCG 1 cut(s) 392
CviAII CATG 3 cut(s) 254, 321, 1089
CviQI GTAC 2 cut(s) 1125, 1131
DdeI CTNAG 3 cut(s) 633, 939, 1011
DpnI GATC 1 cut(s) 714
DpnII GATC 1 cut(s) 712
EaeI YGGCCR 2 cut(s) 183, 261
EagI CGGCCG 1 cut(s) 183
Eam1104I CTCTTC 4 cut(s) 343, 351, 357, 1042
EarI CTCTTC 4 cut(s) 343, 351, 357, 1042
EciI GGCGGA 1 cut(s) 789
EclXI CGGCCG 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 1084
Eco31I GGTCTC 1 cut(s) 636
Eco47I GGWCC 2 cut(s) 392, 1103
Eco52I CGGCCG 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 22
EcoRII CCWGG 2 cut(s) 491, 659
EcoT38I GRGCYC 1 cut(s) 1084
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 3 cut(s) 257, 324, 1092
FaqI GGGAC 1 cut(s) 90
FatI CATG 3 cut(s) 253, 320, 1088
FauI CCCGC 1 cut(s) 141
FblI GTMKAC 1 cut(s) 552
FokI GGATG 4 cut(s) 448, 841, 982, 1093
FriOI GRGCYC 1 cut(s) 1084
FspBI CTAG 3 cut(s) 315, 1128, 1161
GlaI GCGC 2 cut(s) 59, 561
HaeIII GGCC 5 cut(s) 185, 245, 263, 460, 746
HapII CCGG 1 cut(s) 264
HgaI GACGC 2 cut(s) 168, 259
HhaI GCGC 2 cut(s) 60, 562
Hin1II CATG 3 cut(s) 257, 324, 1092
Hin6I GCGC 2 cut(s) 58, 560
HinP1I GCGC 2 cut(s) 58, 560
HincII GTYRAC 1 cut(s) 786
HindII GTYRAC 1 cut(s) 786
HinfI GANTC 4 cut(s) 305, 404, 729, 774
HpaII CCGG 1 cut(s) 264
HphI GGTGA 1 cut(s) 1044
Hpy166II GTNNAC 3 cut(s) 553, 786, 874
Hpy188I TCNGA 8 cut(s) 304, 403, 728, 734, 773, 898, 940, 1103
Hpy188III TCNNGA 3 cut(s) 27, 918, 1191
Hpy8I GTNNAC 3 cut(s) 553, 786, 874
Hpy99I CGWCG 4 cut(s) 184, 898, 1103, 1192
HpyAV CCTTC 4 cut(s) 365, 473, 658, 918
HpyCH4III ACNGT 4 cut(s) 557, 615, 1124, 1135
HpyCH4IV ACGT 2 cut(s) 46, 768
HpyCH4V TGCA 4 cut(s) 205, 290, 955, 1150
HpyF10VI GCNNNNNNNGC 8 cut(s) 145, 185, 381, 384, 387, 695, 704, 1016
HpyF3I CTNAG 3 cut(s) 633, 939, 1011
HpySE526I ACGT 2 cut(s) 46, 768
Hsp92II CATG 3 cut(s) 257, 324, 1092
HspAI GCGC 2 cut(s) 58, 560
Kzo9I GATC 1 cut(s) 712
LmnI GCTCC 2 cut(s) 799, 829
Lsp1109I GCAGC 8 cut(s) 271, 274, 616, 643, 706, 1003, 1006, 1162
LweI GCATC 1 cut(s) 197
MaeI CTAG 3 cut(s) 315, 1128, 1161
MaeII ACGT 2 cut(s) 46, 768
MaeIII GTNAC 2 cut(s) 409, 1115
MalI GATC 1 cut(s) 714
MbiI CCGCTC 1 cut(s) 794
MboI GATC 1 cut(s) 712
MfeI CAATTG 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 1084
MluCI AATT 5 cut(s) 22, 206, 298, 437, 817
MlyI GAGTC 2 cut(s) 413, 768
MmeI TCCRAC 5 cut(s) 313, 542, 554, 876, 1081
MseI TTAA 3 cut(s) 11, 297, 485
MspA1I CMGCKG 1 cut(s) 148
MspCI CTTAAG 1 cut(s) 484
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 2 cut(s) 493, 661
MunI CAATTG 1 cut(s) 206
Mva1269I GAATGC 1 cut(s) 884
MvaI CCWGG 2 cut(s) 493, 661
MwoI GCNNNNNNNGC 8 cut(s) 145, 185, 381, 384, 387, 695, 704, 1016
NdeII GATC 1 cut(s) 712
NlaIII CATG 3 cut(s) 257, 324, 1092
NlaIV GGNNCC 3 cut(s) 459, 676, 906
NmuCI GTSAC 1 cut(s) 1115
NspI RCATGY 1 cut(s) 257
PaqCI CACCTGC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 2 cut(s) 188, 1107
PctI GAATGC 1 cut(s) 884
PfeI GAWTC 2 cut(s) 305, 729
PflMI CCANNNNNTGG 1 cut(s) 666
PleI GAGTC 2 cut(s) 412, 768
PpsI GAGTC 2 cut(s) 412, 768
PshAI GACNNNNGTC 1 cut(s) 778
Psp6I CCWGG 2 cut(s) 491, 659
PspGI CCWGG 2 cut(s) 491, 659
PspN4I GGNNCC 3 cut(s) 459, 676, 906
PspPI GGNCC 3 cut(s) 392, 458, 1103
PstI CTGCAG 1 cut(s) 292
RsaI GTAC 2 cut(s) 1126, 1132
RsaNI GTAC 2 cut(s) 1125, 1131
Rsr2I CGGWCCG 1 cut(s) 392
RsrII CGGWCCG 1 cut(s) 392
SaqAI TTAA 3 cut(s) 11, 297, 485
Sau3AI GATC 1 cut(s) 712
Sau96I GGNCC 3 cut(s) 392, 458, 1103
ScaI AGTACT 2 cut(s) 1126, 1132
SchI GAGTC 2 cut(s) 413, 768
ScrFI CCNGG 2 cut(s) 493, 661
SduI GDGCHC 1 cut(s) 1084
SfaNI GCATC 1 cut(s) 197
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 2 cut(s) 392, 1103
SmlI CTYRAG 3 cut(s) 484, 879, 916
SmoI CTYRAG 3 cut(s) 484, 879, 916
SpeI ACTAGT 2 cut(s) 314, 1127
Sse9I AATT 5 cut(s) 22, 206, 298, 437, 817
SspMI CTAG 3 cut(s) 315, 1128, 1161
StyD4I CCNGG 2 cut(s) 491, 659
TaaI ACNGT 4 cut(s) 557, 615, 1124, 1135
TaiI ACGT 2 cut(s) 49, 771
TaqI TCGA 2 cut(s) 432, 1190
TaqII GACCGA 1 cut(s) 1074
TasI AATT 5 cut(s) 22, 206, 298, 437, 817
TatI WGTACW 2 cut(s) 1124, 1130
TfiI GAWTC 2 cut(s) 305, 729
Tru1I TTAA 3 cut(s) 11, 297, 485
Tru9I TTAA 3 cut(s) 11, 297, 485
TscAI CASTG 2 cut(s) 334, 562
TseFI GTSAC 1 cut(s) 1115
TseI GCWGC 8 cut(s) 284, 287, 629, 656, 719, 1016, 1019, 1150
Tsp45I GTSAC 1 cut(s) 1115
TspDTI ATGAA 3 cut(s) 129, 309, 450
TspGWI ACGGA 4 cut(s) 384, 835, 1050, 1099
TspRI CASTG 2 cut(s) 334, 562
Van91I CCANNNNNTGG 1 cut(s) 666
Vha464I CTTAAG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 392, 1103
XapI RAATTY 2 cut(s) 22, 437
XceI RCATGY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 1152
XmiI GTMKAC 1 cut(s) 552
XspI CTAG 3 cut(s) 315, 1128, 1161
ZrmI AGTACT 2 cut(s) 1126, 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.