RchiOBHm_Chr2g0118251

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011515
Physical Location & Seq
Reverse (-)
30584572 .. 30586600
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49109

Sequence Viewer

Length: 1167 bp
ATGTGTTTTCTAAAGGTGGCGAATCCGAGCAGAGACAGAGACGACGAGCGCGCCGCCGCGGCGGGGCAAGGAAATGAGACCGACCAAACTCATGAACAGAGAGACCTTATCGAAGAGTGGCTGCTATTTGAGCCCCTGCTTTTTTCTCAGCAACAACGACGTGGTGCTGGTGGTAGTAGTGGTGGTGGTGGTGCTGGAGGAATATTAGCTTCGTCGTCAATGCTGTTGCGGTTGGATAGGGAGAGGGAGATGTCGGCAATGGTTTCGGCTCTGACGCATGTGGTGACAGGAGAGGAGCAGACTAGGTTTGATGTGGTTAACGGTTCGGATTCGCCGGCGGGGAATGGTTTGTGCGGTGGCGTTGGAAGTAAGAGAGAAAGAGAAGAGGAAGGAGGTGGTGGTGGACAGAACATGTCTGAGTCGGTTTCAAGGCTTCCACAACAGGGCTCACCTTCATCCTCCTCTGTTTTTCCAGAGAACTCGATCACAAGAACTCGAAGGACTACTAGCACCACACCACCTGTACAAACAGCATATGAATATAGTGCACCTCAATTATCAGAACAACCAATAAGGAGAAAATACAGAGGAGTGAGGCAGAGGCCATGGGGAAAATGGGCGGCTGAGATCAGAGACCCGTTCAAGGCTGCCAGAGTCTGGCTAGGCACATTCGACACAGCAGAAGCCGCCGCGCAAGCCTACGACACAGCCGCCCTGCGCTTCCGAGGCAACAAGGCAAAACTCAACTTTCCGGAAAACGTCAGACTCCGATCACTACCATCATCAGCTGCAGAAAATCCAATGACGACGCAACCTACAGTTTCGGGTTCTTCGAATGCCCTCCTGTCTATTCCTACATCCACCGAACCTATTGTTCACTCCCAAGCAGCTGCTTCATTCCAACAACAACGGCCTATGAATCTTTATGATCAGATGGTTTTGTTATCTTCGTCTTCATCTCCATCAGCTACTTCTTCTTCTACACTCGCCTCCTCTGTTTCCTCTGCTTCTTCTTCGCCACCACCGCCTGCCTTTTCCTCACTTTTTAGTGCTCAGGCAACGTCACCGGTTCAGCTCAGACAACCTACCGGTGATCAGAGTGGCGGAGCTGATTTTTCAGTGCAGCAGCAATGGTCAACGAGCTCCGGTCATTACAGTACTTCCTAG

Protein Analysis

388

Amino Acids

41.39

Weight (kDa)

6.47

Isoelectric Point (pI)

65.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 194 - 243 1.2e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 657
AccII CGCG 3 cut(s) 51, 59, 692
AccIII TCCGGA 1 cut(s) 751
AfaI GTAC 2 cut(s) 525, 1159
AfiI CCNNNNNNNGG 4 cut(s) 63, 443, 643, 657
AflIII ACRYGT 1 cut(s) 411
AgeI ACCGGT 2 cut(s) 1066, 1088
AgsI TTSAA 2 cut(s) 429, 643
AjiI CACGTC 1 cut(s) 161
AluBI AGCT 7 cut(s) 209, 788, 890, 968, 1075, 1109, 1143
AluI AGCT 7 cut(s) 209, 788, 890, 968, 1075, 1109, 1143
Alw21I GWGCWC 3 cut(s) 550, 1054, 1145
Alw26I GTCTC 5 cut(s) 27, 33, 71, 96, 627
Alw44I GTGCAC 1 cut(s) 546
AlwNI CAGNNNCTG 1 cut(s) 657
Aor13HI TCCGGA 1 cut(s) 751
AoxI GGCC 2 cut(s) 602, 911
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 7 cut(s) 121, 647, 788, 887, 890, 1123, 1126
AsiGI ACCGGT 2 cut(s) 1066, 1088
AspLEI GCGC 4 cut(s) 51, 53, 694, 720
AsuHPI GGTGA 4 cut(s) 295, 441, 1056, 1103
AsuII TTCGAA 1 cut(s) 833
BaeGI GKGCMC 1 cut(s) 550
BanII GRGCYC 3 cut(s) 135, 449, 1145
BbsI GAAGAC 1 cut(s) 945
Bbv12I GWGCWC 3 cut(s) 550, 1054, 1145
BbvI GCAGC 7 cut(s) 108, 634, 775, 877, 899, 1135, 1138
BccI CCATC 3 cut(s) 787, 928, 970
BceAI ACGGC 1 cut(s) 926
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BclI TGATCA 2 cut(s) 928, 1093
BcoDI GTCTC 5 cut(s) 27, 33, 71, 96, 627
BfaI CTAG 4 cut(s) 303, 507, 662, 1165
BfmI CTRYAG 2 cut(s) 789, 816
BglI GCCNNNNNGGC 1 cut(s) 59
BmcAI AGTACT 1 cut(s) 1159
BmgBI CACGTC 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 945
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 1 cut(s) 1053
Bpu14I TTCGAA 1 cut(s) 833
BsaI GGTCTC 3 cut(s) 71, 96, 627
BsaJI CCNNGG 3 cut(s) 57, 605, 724
BsaWI WCCGGW 4 cut(s) 751, 1066, 1088, 1145
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 443, 643, 657
Bse118I RCCGGY 3 cut(s) 334, 1066, 1088
Bse3DI GCAATG 2 cut(s) 264, 1136
BseAI TCCGGA 1 cut(s) 751
BseDI CCNNGG 3 cut(s) 57, 605, 724
BseGI GGATG 2 cut(s) 455, 857
BseLI CCNNNNNNNGG 4 cut(s) 63, 443, 643, 657
BseMI GCAATG 2 cut(s) 264, 1136
BseMII CTCAG 5 cut(s) 161, 408, 615, 1067, 1090
BsePI GCGCGC 1 cut(s) 49
BseRI GAGGAG 4 cut(s) 308, 451, 603, 982
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 7 cut(s) 108, 634, 775, 877, 899, 1135, 1138
BsgI GTGCAG 1 cut(s) 1142
Bsh1236I CGCG 3 cut(s) 51, 59, 692
BshFI GGCC 2 cut(s) 604, 913
BshTI ACCGGT 2 cut(s) 1066, 1088
BsiHKAI GWGCWC 3 cut(s) 550, 1054, 1145
BsiSI CCGG 5 cut(s) 335, 752, 1067, 1089, 1146
BslI CCNNNNNNNGG 4 cut(s) 63, 443, 643, 657
BsmAI GTCTC 5 cut(s) 27, 33, 71, 96, 627
BsmBI CGTCTC 1 cut(s) 33
BsmI GAATGC 1 cut(s) 841
BsnI GGCC 2 cut(s) 604, 913
Bso31I GGTCTC 3 cut(s) 71, 96, 627
Bsp119I TTCGAA 1 cut(s) 833
Bsp1286I GDGCHC 5 cut(s) 135, 449, 550, 1054, 1145
Bsp13I TCCGGA 1 cut(s) 751
Bsp1407I TGTACA 1 cut(s) 523
Bsp143I GATC 5 cut(s) 483, 627, 770, 928, 1093
Bsp19I CCATGG 1 cut(s) 605
BspANI GGCC 2 cut(s) 604, 913
BspCNI CTCAG 5 cut(s) 160, 409, 616, 1066, 1089
BspEI TCCGGA 1 cut(s) 751
BspFNI CGCG 3 cut(s) 51, 59, 692
BspHI TCATGA 1 cut(s) 91
BspMAI CTGCAG 1 cut(s) 793
BspT104I TTCGAA 1 cut(s) 833
BspTNI GGTCTC 3 cut(s) 71, 96, 627
BsrDI GCAATG 2 cut(s) 264, 1136
BsrFI RCCGGY 3 cut(s) 334, 1066, 1088
BsrGI TGTACA 1 cut(s) 523
BssAI RCCGGY 3 cut(s) 334, 1066, 1088
BssECI CCNNGG 3 cut(s) 57, 605, 724
BssHII GCGCGC 1 cut(s) 49
BssMI GATC 5 cut(s) 483, 627, 770, 928, 1093
BssT1I CCWWGG 1 cut(s) 605
Bst4CI ACNGT 3 cut(s) 323, 820, 1157
Bst6I CTCTTC 2 cut(s) 108, 378
BstAUI TGTACA 1 cut(s) 523
BstBI TTCGAA 1 cut(s) 833
BstC8I GCNNGC 4 cut(s) 51, 336, 696, 1029
BstDEI CTNAG 5 cut(s) 147, 417, 624, 1053, 1076
BstDSI CCRYGG 2 cut(s) 57, 605
BstF5I GGATG 2 cut(s) 455, 857
BstFNI CGCG 3 cut(s) 51, 59, 692
BstHHI GCGC 4 cut(s) 51, 53, 694, 720
BstKTI GATC 5 cut(s) 486, 630, 773, 931, 1096
BstMAI GTCTC 5 cut(s) 27, 33, 71, 96, 627
BstMBI GATC 5 cut(s) 483, 627, 770, 928, 1093
BstMWI GCNNNNNNNGC 6 cut(s) 59, 130, 686, 695, 726, 1024
BstNSI RCATGY 2 cut(s) 281, 415
BstSFI CTRYAG 2 cut(s) 789, 816
BstSLI GKGCMC 1 cut(s) 550
BstUI CGCG 3 cut(s) 51, 59, 692
BstV1I GCAGC 7 cut(s) 108, 634, 775, 877, 899, 1135, 1138
BstV2I GAAGAC 1 cut(s) 945
BsuRI GGCC 2 cut(s) 604, 913
BtgI CCRYGG 2 cut(s) 57, 605
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 2 cut(s) 455, 857
BtsIMutI CAGTG 1 cut(s) 1125
Cac8I GCNNGC 4 cut(s) 51, 336, 696, 1029
CaiI CAGNNNCTG 1 cut(s) 657
CciI TCATGA 1 cut(s) 91
CfoI GCGC 4 cut(s) 51, 53, 694, 720
Cfr10I RCCGGY 3 cut(s) 334, 1066, 1088
Cfr42I CCGCGG 1 cut(s) 60
CseI GACGC 2 cut(s) 283, 817
Csp6I GTAC 2 cut(s) 524, 1158
CspAI ACCGGT 2 cut(s) 1066, 1088
CviAII CATG 4 cut(s) 92, 278, 412, 606
CviQI GTAC 2 cut(s) 524, 1158
DdeI CTNAG 5 cut(s) 147, 417, 624, 1053, 1076
DpnI GATC 5 cut(s) 485, 629, 772, 930, 1095
DpnII GATC 5 cut(s) 483, 627, 770, 928, 1093
Eam1104I CTCTTC 2 cut(s) 108, 378
EarI CTCTTC 2 cut(s) 108, 378
EciI GGCGGA 1 cut(s) 1119
Ecl136II GAGCTC 1 cut(s) 1143
Eco130I CCWWGG 1 cut(s) 605
Eco24I GRGCYC 3 cut(s) 135, 449, 1145
Eco31I GGTCTC 3 cut(s) 71, 96, 627
Eco53kI GAGCTC 1 cut(s) 1143
EcoICRI GAGCTC 1 cut(s) 1143
EcoT14I CCWWGG 1 cut(s) 605
EcoT38I GRGCYC 3 cut(s) 135, 449, 1145
ErhI CCWWGG 1 cut(s) 605
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 4 cut(s) 95, 281, 415, 609
FaiI YATR 9 cut(s) 93, 279, 413, 535, 537, 543, 607, 917, 927
FatI CATG 4 cut(s) 91, 277, 411, 605
FauI CCCGC 2 cut(s) 55, 331
FauNDI CATATG 1 cut(s) 535
FbaI TGATCA 2 cut(s) 928, 1093
FokI GGATG 2 cut(s) 442, 844
FriOI GRGCYC 3 cut(s) 135, 449, 1145
FspBI CTAG 4 cut(s) 303, 507, 662, 1165
GlaI GCGC 4 cut(s) 50, 52, 693, 719
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 604, 913
HapII CCGG 5 cut(s) 335, 752, 1067, 1089, 1146
HgaI GACGC 2 cut(s) 283, 817
HhaI GCGC 4 cut(s) 51, 53, 694, 720
Hin1II CATG 4 cut(s) 95, 281, 415, 609
Hin6I GCGC 4 cut(s) 49, 51, 692, 718
HinP1I GCGC 4 cut(s) 49, 51, 692, 718
HincII GTYRAC 2 cut(s) 319, 1137
HindII GTYRAC 2 cut(s) 319, 1137
HinfI GANTC 6 cut(s) 22, 329, 419, 654, 765, 919
HpaI GTTAAC 1 cut(s) 319
HpaII CCGG 5 cut(s) 335, 752, 1067, 1089, 1146
HphI GGTGA 4 cut(s) 295, 441, 1056, 1103
Hpy166II GTNNAC 5 cut(s) 319, 404, 548, 877, 1137
Hpy188III TCNNGA 3 cut(s) 92, 473, 752
Hpy8I GTNNAC 5 cut(s) 319, 404, 548, 877, 1137
Hpy99I CGWCG 4 cut(s) 47, 162, 217, 811
HpyAV CCTTC 3 cut(s) 383, 462, 492
HpyCH4III ACNGT 3 cut(s) 323, 820, 1157
HpyCH4IV ACGT 3 cut(s) 160, 759, 1061
HpyCH4V TGCA 3 cut(s) 548, 791, 1123
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 130, 686, 695, 726, 1024
HpyF3I CTNAG 5 cut(s) 147, 417, 624, 1053, 1076
HpySE526I ACGT 3 cut(s) 160, 759, 1061
Hsp92II CATG 4 cut(s) 95, 281, 415, 609
HspAI GCGC 4 cut(s) 49, 51, 692, 718
Kpn2I TCCGGA 1 cut(s) 751
KroI GCCGGC 1 cut(s) 334
KroNI GCCGGC 1 cut(s) 336
Ksp22I TGATCA 2 cut(s) 928, 1093
KspAI GTTAAC 1 cut(s) 319
KspI CCGCGG 1 cut(s) 60
Kzo9I GATC 5 cut(s) 483, 627, 770, 928, 1093
LmnI GCTCC 3 cut(s) 295, 1106, 1148
Lsp1109I GCAGC 7 cut(s) 108, 634, 775, 877, 899, 1135, 1138
MaeI CTAG 4 cut(s) 303, 507, 662, 1165
MaeII ACGT 3 cut(s) 160, 759, 1061
MaeIII GTNAC 2 cut(s) 283, 1062
MalI GATC 5 cut(s) 485, 629, 772, 930, 1095
MboI GATC 5 cut(s) 483, 627, 770, 928, 1093
MboII GAAGA 9 cut(s) 125, 395, 822, 939, 945, 966, 969, 1002, 1005
MhlI GDGCHC 5 cut(s) 135, 449, 550, 1054, 1145
MluCI AATT 1 cut(s) 554
MlyI GAGTC 3 cut(s) 428, 663, 759
MmeI TCCRAC 3 cut(s) 213, 343, 925
MreI CGCCGGCG 1 cut(s) 334
MroI TCCGGA 1 cut(s) 751
MroNI GCCGGC 1 cut(s) 334
MseI TTAA 1 cut(s) 318
MspA1I CMGCKG 3 cut(s) 59, 788, 890
MspI CCGG 5 cut(s) 335, 752, 1067, 1089, 1146
Mva1269I GAATGC 1 cut(s) 841
MvnI CGCG 3 cut(s) 51, 59, 692
MwoI GCNNNNNNNGC 6 cut(s) 59, 130, 686, 695, 726, 1024
NaeI GCCGGC 1 cut(s) 336
NcoI CCATGG 1 cut(s) 605
NdeI CATATG 1 cut(s) 535
NdeII GATC 5 cut(s) 483, 627, 770, 928, 1093
NgoMIV GCCGGC 1 cut(s) 334
NlaIII CATG 4 cut(s) 95, 281, 415, 609
NmuCI GTSAC 2 cut(s) 283, 1062
NspI RCATGY 2 cut(s) 281, 415
NspV TTCGAA 1 cut(s) 833
PagI TCATGA 1 cut(s) 91
PauI GCGCGC 1 cut(s) 49
PciI ACATGT 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 830
PctI GAATGC 1 cut(s) 841
PdiI GCCGGC 1 cut(s) 336
PfeI GAWTC 3 cut(s) 22, 329, 919
PflMI CCANNNNNTGG 1 cut(s) 657
PinAI ACCGGT 2 cut(s) 1066, 1088
PleI GAGTC 3 cut(s) 427, 662, 759
PpsI GAGTC 3 cut(s) 427, 662, 759
PscI ACATGT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 1145
PstI CTGCAG 1 cut(s) 793
PstNI CAGNNNCTG 1 cut(s) 657
PteI GCGCGC 1 cut(s) 49
PvuII CAGCTG 2 cut(s) 788, 890
RsaI GTAC 2 cut(s) 525, 1159
RsaNI GTAC 2 cut(s) 524, 1158
SacI GAGCTC 1 cut(s) 1145
SacII CCGCGG 1 cut(s) 60
SaqAI TTAA 1 cut(s) 318
Sau3AI GATC 5 cut(s) 483, 627, 770, 928, 1093
ScaI AGTACT 1 cut(s) 1159
SchI GAGTC 3 cut(s) 428, 663, 759
SduI GDGCHC 5 cut(s) 135, 449, 550, 1054, 1145
SfcI CTRYAG 2 cut(s) 789, 816
Sfr303I CCGCGG 1 cut(s) 60
SfuI TTCGAA 1 cut(s) 833
SgrAI CRCCGGYG 1 cut(s) 334
SgrBI CCGCGG 1 cut(s) 60
Sse9I AATT 1 cut(s) 554
SspI AATATT 1 cut(s) 204
SspMI CTAG 4 cut(s) 303, 507, 662, 1165
SstI GAGCTC 1 cut(s) 1145
StyI CCWWGG 1 cut(s) 605
TaaI ACNGT 3 cut(s) 323, 820, 1157
TaiI ACGT 3 cut(s) 163, 762, 1064
TaqI TCGA 5 cut(s) 111, 482, 496, 672, 833
TaqII GACCGA 1 cut(s) 95
TasI AATT 1 cut(s) 554
TatI WGTACW 2 cut(s) 523, 1157
TauI GCSGC 7 cut(s) 56, 59, 62, 623, 689, 692, 713
TfiI GAWTC 3 cut(s) 22, 329, 919
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 1 cut(s) 1125
TseFI GTSAC 2 cut(s) 283, 1062
TseI GCWGC 7 cut(s) 121, 647, 788, 887, 890, 1123, 1126
Tsp45I GTSAC 2 cut(s) 283, 1062
TspDTI ATGAA 6 cut(s) 108, 444, 552, 885, 932, 945
TspRI CASTG 1 cut(s) 1125
Van91I CCANNNNNTGG 1 cut(s) 657
VneI GTGCAC 1 cut(s) 546
XceI RCATGY 2 cut(s) 281, 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 612
XspI CTAG 4 cut(s) 303, 507, 662, 1165
ZrmI AGTACT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.