FvH4_1g23500

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002647
Physical Location & Seq
Reverse (-)
15344441 .. 15345127
687 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23500.t1

Sequence Viewer

Length: 687 bp
ATGGCTGAAGAGGACTCAACCACGCAGCAAGAACATATCGCGGAAGAGGTGGAAGAGAAGCAGCAGCAGCAGCTCAAGTACCTGCAATTTGTTCAAGTGGCTGCACTTCACGCCGCGGTCCGCTTCTCAAATGCTTATGGTTATGCCAAAGAGAAATCCGGTCCGTTGAGGACCGGCGTTGAGTCAGCAGAGGGAGCTGTGAAAACTGTTGTTGGACCTGTGTATGACAAGTACCAGGCTCTCCCTGGCGACCTCCTCTCTTTTCTTGATGGCAAGGTTGACGAATCTGTGACCAATCTGGCATCATCCTATGCAGACGCTGCTCAGAAAGCGCCAGATGCTGCTCGAGCTGCTGCATTAGAGATCAAGCGAAGAAGCTCAACCATGGCTAAGTCGGTATACTCCAAGTACGAACCGAAAGCTCAGCAATGTGCTGTTTGTGCATGGAGAAAGCTGAATGAACTACCTGGGGGGCCAAAGGTGGCGGAAATTGTTGTACCAACCGCTGCATATTGTTCGGAGAAGTACAACCACACTGTGAGTTGCGCTGTGGAGAAGGGGTACAAGGTTGCCTCCTATTTGCCTTTAGTTCCAACAGAGAAAATTGCACAGGTTTTTGGAGGAATGTGCCAGAAGCCGTTCAGTGATAGCTACTTCATTGTTGGTTTTTTTTTATGGCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

229

Amino Acids

24.94

Weight (kDa)

6.33

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 19 - 207 2.5e-67 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 399
AccII CGCG 2 cut(s) 41, 116
AciI CCGC 6 cut(s) 41, 114, 116, 121, 485, 504
AcuI CTGAAG 1 cut(s) 27
AdeI CACNNNGTG 1 cut(s) 538
AfaI GTAC 6 cut(s) 80, 233, 410, 498, 527, 563
AgsI TTSAA 1 cut(s) 95
AjnI CCWGG 3 cut(s) 234, 244, 466
AluBI AGCT 7 cut(s) 73, 197, 350, 378, 422, 454, 651
AluI AGCT 7 cut(s) 73, 197, 350, 378, 422, 454, 651
AlwNI CAGNNNCTG 2 cut(s) 320, 341
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 2 cut(s) 473, 677
Asp700I GAANNNNTTC 1 cut(s) 638
AspLEI GCGC 2 cut(s) 334, 548
AspS9I GGNCC 5 cut(s) 118, 161, 171, 215, 473
AvaI CYCGRG 1 cut(s) 345
AvaII GGWCC 4 cut(s) 118, 161, 171, 215
BccI CCATC 1 cut(s) 263
BceAI ACGGC 1 cut(s) 622
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BciT130I CCWGG 3 cut(s) 236, 246, 468
BfoI RGCGCY 1 cut(s) 335
BfuAI ACCTGC 1 cut(s) 90
BlpI GCTNAGC 1 cut(s) 423
Bme1390I CCNGG 3 cut(s) 236, 246, 468
Bme18I GGWCC 4 cut(s) 118, 161, 171, 215
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 5 cut(s) 118, 161, 171, 215, 473
BmiI GGNNCC 1 cut(s) 474
BmrFI CCNGG 3 cut(s) 236, 246, 468
BmsI GCATC 2 cut(s) 311, 328
Bpu1102I GCTNAGC 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 59
BsaJI CCNNGG 4 cut(s) 114, 244, 384, 467
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 434
BseBI CCWGG 3 cut(s) 236, 246, 468
BseDI CCNNGG 4 cut(s) 114, 244, 384, 467
BseGI GGATG 1 cut(s) 305
BseMI GCAATG 1 cut(s) 434
BseMII CTCAG 2 cut(s) 338, 437
BseRI GAGGAG 1 cut(s) 245
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 2 cut(s) 41, 116
BshFI GGCC 2 cut(s) 475, 679
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 2 cut(s) 159, 174
BsnI GGCC 2 cut(s) 475, 679
BsoBI CYCGRG 1 cut(s) 345
Bsp143I GATC 1 cut(s) 363
Bsp1720I GCTNAGC 1 cut(s) 423
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 6 cut(s) 41, 114, 116, 121, 485, 504
BspANI GGCC 2 cut(s) 475, 679
BspCNI CTCAG 2 cut(s) 337, 436
BspFNI CGCG 2 cut(s) 41, 116
BspLI GGNNCC 1 cut(s) 474
BspMI ACCTGC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 434
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 4 cut(s) 114, 244, 384, 467
BssMI GATC 1 cut(s) 363
BssNAI GTATAC 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 384
Bst1107I GTATAC 1 cut(s) 400
Bst2UI CCWGG 3 cut(s) 236, 246, 468
Bst4CI ACNGT 2 cut(s) 208, 538
Bst6I CTCTTC 3 cut(s) 3, 39, 48
BstAPI GCANNNNNTGC 1 cut(s) 320
BstDEI CTNAG 3 cut(s) 324, 390, 423
BstDSI CCRYGG 2 cut(s) 114, 384
BstF5I GGATG 1 cut(s) 305
BstFNI CGCG 2 cut(s) 41, 116
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 2 cut(s) 334, 548
BstKTI GATC 1 cut(s) 366
BstMBI GATC 1 cut(s) 363
BstNI CCWGG 3 cut(s) 236, 246, 468
BstSCI CCNGG 3 cut(s) 234, 244, 466
BstUI CGCG 2 cut(s) 41, 116
BstZ17I GTATAC 1 cut(s) 400
BsuRI GGCC 2 cut(s) 475, 679
BtgI CCRYGG 2 cut(s) 114, 384
BtsCI GGATG 1 cut(s) 305
BtsIMutI CAGTG 2 cut(s) 534, 649
BveI ACCTGC 1 cut(s) 90
CaiI CAGNNNCTG 2 cut(s) 320, 341
CfoI GCGC 2 cut(s) 334, 548
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 5 cut(s) 118, 161, 171, 215, 473
Cfr42I CCGCGG 1 cut(s) 117
CpoI CGGWCCG 2 cut(s) 118, 161
CseI GACGC 1 cut(s) 326
Csp6I GTAC 6 cut(s) 79, 232, 409, 497, 526, 562
CspI CGGWCCG 2 cut(s) 118, 161
CviAII CATG 2 cut(s) 385, 444
CviQI GTAC 6 cut(s) 79, 232, 409, 497, 526, 562
DdeI CTNAG 3 cut(s) 324, 390, 423
DpnI GATC 1 cut(s) 365
DpnII GATC 1 cut(s) 363
DraIII CACNNNGTG 1 cut(s) 538
Eam1104I CTCTTC 3 cut(s) 3, 39, 48
EarI CTCTTC 3 cut(s) 3, 39, 48
EciI GGCGGA 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 384
Eco47I GGWCC 4 cut(s) 118, 161, 171, 215
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 345
EcoRII CCWGG 3 cut(s) 234, 244, 466
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 2 cut(s) 388, 447
FatI CATG 2 cut(s) 384, 443
FblI GTMKAC 1 cut(s) 399
FokI GGATG 1 cut(s) 292
GlaI GCGC 2 cut(s) 333, 547
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 2 cut(s) 475, 679
HapII CCGG 2 cut(s) 159, 174
HgaI GACGC 1 cut(s) 326
HhaI GCGC 2 cut(s) 334, 548
Hin1II CATG 2 cut(s) 388, 447
Hin6I GCGC 2 cut(s) 332, 546
HinP1I GCGC 2 cut(s) 332, 546
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 3 cut(s) 14, 182, 284
HpaII CCGG 2 cut(s) 159, 174
Hpy166II GTNNAC 2 cut(s) 280, 400
Hpy188I TCNGA 2 cut(s) 327, 520
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 2 cut(s) 280, 400
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 2 cut(s) 208, 538
HpyCH4V TGCA 7 cut(s) 85, 104, 314, 356, 443, 509, 608
HpyF3I CTNAG 3 cut(s) 324, 390, 423
Hsp92II CATG 2 cut(s) 388, 447
HspAI GCGC 2 cut(s) 332, 546
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 1 cut(s) 363
LmnI GCTCC 1 cut(s) 194
LweI GCATC 2 cut(s) 311, 328
MaeIII GTNAC 1 cut(s) 289
MalI GATC 1 cut(s) 365
MboI GATC 1 cut(s) 363
MboII GAAGA 4 cut(s) 20, 56, 65, 384
MluCI AATT 3 cut(s) 86, 489, 603
MlyI GAGTC 2 cut(s) 8, 191
MmeI TCCRAC 2 cut(s) 193, 617
MnlI CCTC 8 cut(s) 4, 40, 162, 184, 263, 266, 583, 614
MroXI GAANNNNTTC 1 cut(s) 638
MspA1I CMGCKG 2 cut(s) 116, 506
MspI CCGG 2 cut(s) 159, 174
MspR9I CCNGG 3 cut(s) 236, 246, 468
MvaI CCWGG 3 cut(s) 236, 246, 468
MvnI CGCG 2 cut(s) 41, 116
NcoI CCATGG 1 cut(s) 384
NdeII GATC 1 cut(s) 363
NlaIII CATG 2 cut(s) 388, 447
NlaIV GGNNCC 1 cut(s) 474
NmuCI GTSAC 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 345
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 1 cut(s) 284
PleI GAGTC 2 cut(s) 8, 190
PpsI GAGTC 2 cut(s) 8, 190
Psp6I CCWGG 3 cut(s) 234, 244, 466
PspGI CCWGG 3 cut(s) 234, 244, 466
PspN4I GGNNCC 1 cut(s) 474
PspPI GGNCC 5 cut(s) 118, 161, 171, 215, 473
PspXI VCTCGAGB 1 cut(s) 345
PstNI CAGNNNCTG 2 cut(s) 320, 341
RsaI GTAC 6 cut(s) 80, 233, 410, 498, 527, 563
RsaNI GTAC 6 cut(s) 79, 232, 409, 497, 526, 562
Rsr2I CGGWCCG 2 cut(s) 118, 161
RsrII CGGWCCG 2 cut(s) 118, 161
SacII CCGCGG 1 cut(s) 117
Sau3AI GATC 1 cut(s) 363
Sau96I GGNCC 5 cut(s) 118, 161, 171, 215, 473
SchI GAGTC 2 cut(s) 8, 191
ScrFI CCNGG 3 cut(s) 236, 246, 468
SfaNI GCATC 2 cut(s) 311, 328
Sfr274I CTCGAG 1 cut(s) 345
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
SinI GGWCC 4 cut(s) 118, 161, 171, 215
SlaI CTCGAG 1 cut(s) 345
SmlI CTYRAG 2 cut(s) 74, 345
SmoI CTYRAG 2 cut(s) 74, 345
Sse9I AATT 3 cut(s) 86, 489, 603
SsiI CCGC 6 cut(s) 41, 114, 116, 121, 485, 504
StyD4I CCNGG 3 cut(s) 234, 244, 466
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 2 cut(s) 208, 538
TaqI TCGA 1 cut(s) 346
TasI AATT 3 cut(s) 86, 489, 603
TatI WGTACW 1 cut(s) 525
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 1 cut(s) 284
TscAI CASTG 2 cut(s) 541, 649
TseFI GTSAC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 2 cut(s) 474, 646
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 2 cut(s) 541, 649
VpaK11BI GGWCC 4 cut(s) 118, 161, 171, 215
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XhoI CTCGAG 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.