RLG00000018434

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002647
Physical Location & Seq
Reverse (-)
29722048 .. 29722692
645 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018434

Sequence Viewer

Length: 645 bp
ATGGCTCAAGAGGACTTGACCACGCAGCAAAAACAGATGGCGGAGGAGGTGGAAGAGCAGCAGCGGCTCAAGTACCTGCAATTTGTTCAAGTGGCTGCACTTCACGCCGCGGTTTGCTTCTCAAATGCTTATGGTTATGCCAAGGAGAAATCCGGCCCTCTGAGGCCCGGCGTTGAGTCAGCGGAGGGAGCTGTGAAGACTGTTGTTGGACCTGTGTATGACAAGTACCAGGCTCTCCCCGCCAACCTCCTCGCATTCGTTGATGGCCAGGTCGACGAATCTGTGACCAAGCTGGCTTCATCCCAAGCAATGTCTGCTGCTCAGAAAGCCCCAGAGGCTGCTCGAGCTGTTGCATCGGAGTTTCAGCAAAGGAGCACAAGCTTGGCTAAGTCGGTATACTCCAAGTACGAACCGAAAGCTGAGCAATGTGCTGTTTGTGCATGGAGAAAGCTGAATGAGCTACCTGTGGTACCGAAGGTGGCTGAGATTGTTGTACCAACTGCTGCATATTGTTCCGAAAAGTACAACCACAGTGTGCGTTGCAGTGAGGAGAAGGGGTACAGGGTGGCCTGCTACTTGCCTTTGGTTCCAACAGAGAAAATTGCTAAGGTTTTTGGAGGGAATGTGCCTGAAGCTGTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

215

Amino Acids

23.31

Weight (kDa)

6.74

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 18 - 206 1.1e-73 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 84
Acc65I GGTACC 1 cut(s) 469
AccB1I GGYRCC 1 cut(s) 469
AccI GTMKAC 2 cut(s) 273, 396
AccII CGCG 1 cut(s) 110
AciI CCGC 6 cut(s) 41, 64, 108, 110, 182, 240
AcoI YGGCCR 1 cut(s) 265
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 7 cut(s) 74, 227, 407, 471, 495, 524, 560
AgsI TTSAA 1 cut(s) 89
AjnI CCWGG 2 cut(s) 228, 267
AluBI AGCT 8 cut(s) 191, 292, 347, 381, 419, 451, 460, 635
AluI AGCT 8 cut(s) 191, 292, 347, 381, 419, 451, 460, 635
Alw21I GWGCWC 1 cut(s) 377
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 342
AoxI GGCC 4 cut(s) 154, 164, 265, 567
ApeKI GCWGC 7 cut(s) 25, 58, 61, 95, 317, 338, 503
Asp700I GAANNNNTTC 1 cut(s) 636
Asp718I GGTACC 1 cut(s) 469
AspS9I GGNCC 3 cut(s) 155, 165, 209
AsuC2I CCSGG 1 cut(s) 168
AvaI CYCGRG 1 cut(s) 342
AvaII GGWCC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 267
BanI GGYRCC 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 7 cut(s) 37, 70, 73, 82, 304, 325, 490
BccI CCATC 2 cut(s) 31, 257
BciT130I CCWGG 2 cut(s) 230, 269
BcnI CCSGG 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 335
BisI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 9 cut(s) 27, 60, 63, 66, 97, 109, 319, 340, 505
Bme1390I CCNGG 3 cut(s) 168, 230, 269
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 342
BmgT120I GGNCC 3 cut(s) 155, 165, 209
BmiI GGNNCC 2 cut(s) 471, 588
BmrFI CCNGG 3 cut(s) 168, 230, 269
BmsI GCATC 1 cut(s) 362
BpiI GAAGAC 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 108, 141
BsaXI ACNNNNNCTCC 4 cut(s) 542, 572, 609, 639
Bse3DI GCAATG 2 cut(s) 315, 431
BseBI CCWGG 2 cut(s) 230, 269
BseDI CCNNGG 2 cut(s) 108, 141
BseGI GGATG 1 cut(s) 299
BseMI GCAATG 2 cut(s) 315, 431
BseMII CTCAG 4 cut(s) 152, 335, 411, 474
BseRI GAGGAG 3 cut(s) 59, 239, 563
BseXI GCAGC 7 cut(s) 37, 70, 73, 82, 304, 325, 490
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 4 cut(s) 156, 166, 267, 569
BshNI GGYRCC 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 377
BsiHKCI CYCGRG 1 cut(s) 342
BsiSI CCGG 2 cut(s) 153, 168
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 4 cut(s) 156, 166, 267, 569
BsoBI CYCGRG 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 377
Bsp1720I GCTNAGC 1 cut(s) 420
BspACI CCGC 6 cut(s) 41, 64, 108, 110, 182, 240
BspANI GGCC 4 cut(s) 156, 166, 267, 569
BspCNI CTCAG 4 cut(s) 153, 334, 412, 475
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 2 cut(s) 471, 588
BspMI ACCTGC 1 cut(s) 84
BspQI GCTCTTC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 469
BsrDI GCAATG 2 cut(s) 315, 431
BssECI CCNNGG 2 cut(s) 108, 141
BssNAI GTATAC 1 cut(s) 397
BssT1I CCWWGG 1 cut(s) 141
Bst1107I GTATAC 1 cut(s) 397
Bst2UI CCWGG 2 cut(s) 230, 269
Bst4CI ACNGT 2 cut(s) 202, 533
Bst6I CTCTTC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 314
BstC8I GCNNGC 2 cut(s) 294, 571
BstDEI CTNAG 6 cut(s) 161, 321, 387, 420, 483, 606
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 299
BstFNI CGCG 1 cut(s) 110
BstNI CCWGG 2 cut(s) 230, 269
BstSCI CCNGG 3 cut(s) 166, 228, 267
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 7 cut(s) 37, 70, 73, 82, 304, 325, 490
BstV2I GAAGAC 1 cut(s) 203
BstZ17I GTATAC 1 cut(s) 397
BsuRI GGCC 4 cut(s) 156, 166, 267, 569
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 299
BtsI GCAGTG 1 cut(s) 550
BtsIMutI CAGTG 2 cut(s) 538, 550
BveI ACCTGC 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 294, 571
CaiI CAGNNNCTG 1 cut(s) 338
Cfr13I GGNCC 3 cut(s) 155, 165, 209
Cfr42I CCGCGG 1 cut(s) 111
Csp6I GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
CviAII CATG 1 cut(s) 441
CviQI GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
DdeI CTNAG 6 cut(s) 161, 321, 387, 420, 483, 606
DraIII CACNNNGTG 1 cut(s) 535
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
EciI GGCGGA 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 228, 267
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 1 cut(s) 444
FaiI YATR 6 cut(s) 132, 138, 219, 397, 442, 508
FatI CATG 1 cut(s) 440
FauI CCCGC 1 cut(s) 247
FblI GTMKAC 2 cut(s) 273, 396
Fnu4HI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
GluI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
HaeIII GGCC 4 cut(s) 156, 166, 267, 569
HapII CCGG 2 cut(s) 153, 168
Hin1II CATG 1 cut(s) 444
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 2 cut(s) 176, 278
HpaII CCGG 2 cut(s) 153, 168
Hpy166II GTNNAC 2 cut(s) 274, 397
Hpy188I TCNGA 4 cut(s) 162, 324, 358, 517
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 2 cut(s) 274, 397
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 469, 547
HpyCH4III ACNGT 2 cut(s) 202, 533
HpyCH4V TGCA 6 cut(s) 79, 98, 353, 440, 506, 543
HpyF3I CTNAG 6 cut(s) 161, 321, 387, 420, 483, 606
Hsp92II CATG 1 cut(s) 444
KpnI GGTACC 1 cut(s) 473
KspI CCGCGG 1 cut(s) 111
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 2 cut(s) 188, 372
Lsp1109I GCAGC 7 cut(s) 37, 70, 73, 82, 304, 325, 490
LweI GCATC 1 cut(s) 362
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 2 cut(s) 65, 208
MhlI GDGCHC 1 cut(s) 377
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 2 cut(s) 80, 600
MluNI TGGCCA 1 cut(s) 267
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 2 cut(s) 187, 614
Mox20I TGGCCA 1 cut(s) 267
MroXI GAANNNNTTC 1 cut(s) 636
MscI TGGCCA 1 cut(s) 267
Msp20I TGGCCA 1 cut(s) 267
MspA1I CMGCKG 3 cut(s) 64, 110, 182
MspI CCGG 2 cut(s) 153, 168
MspR9I CCNGG 3 cut(s) 168, 230, 269
Mva1269I GAATGC 1 cut(s) 254
MvaI CCWGG 2 cut(s) 230, 269
MvnI CGCG 1 cut(s) 110
NciI CCSGG 1 cut(s) 168
NlaIII CATG 1 cut(s) 444
NlaIV GGNNCC 2 cut(s) 471, 588
NmuCI GTSAC 1 cut(s) 283
PaeR7I CTCGAG 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 9 cut(s) 27, 60, 63, 66, 97, 109, 319, 340, 505
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 228, 267
PspGI CCWGG 2 cut(s) 228, 267
PspN4I GGNNCC 2 cut(s) 471, 588
PspPI GGNCC 3 cut(s) 155, 165, 209
PspXI VCTCGAGB 1 cut(s) 342
PstNI CAGNNNCTG 1 cut(s) 338
RsaI GTAC 7 cut(s) 74, 227, 407, 471, 495, 524, 560
RsaNI GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
SacII CCGCGG 1 cut(s) 111
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 48
SatI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
Sau96I GGNCC 3 cut(s) 155, 165, 209
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 168, 230, 269
SduI GDGCHC 1 cut(s) 377
SfaNI GCATC 1 cut(s) 362
Sfr274I CTCGAG 1 cut(s) 342
Sfr303I CCGCGG 1 cut(s) 111
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 342
SmlI CTYRAG 3 cut(s) 6, 68, 342
SmoI CTYRAG 3 cut(s) 6, 68, 342
Sse9I AATT 2 cut(s) 80, 600
SsiI CCGC 6 cut(s) 41, 64, 108, 110, 182, 240
StyD4I CCNGG 3 cut(s) 166, 228, 267
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 202, 533
TaqI TCGA 2 cut(s) 273, 343
TasI AATT 2 cut(s) 80, 600
TatI WGTACW 1 cut(s) 522
TauI GCSGC 2 cut(s) 67, 110
TfiI GAWTC 1 cut(s) 278
TscAI CASTG 2 cut(s) 538, 550
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 7 cut(s) 25, 58, 61, 95, 317, 338, 503
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 288
TspRI CASTG 2 cut(s) 538, 550
VpaK11BI GGWCC 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 342
XmiI GTMKAC 2 cut(s) 273, 396
XmnI GAANNNNTTC 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.