Rh2BG288400

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002647
Physical Location & Seq
Reverse (-)
31978574 .. 31979770
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG288400.1

Sequence Viewer

Length: 642 bp
ATGGCTCAAGAGGACTTGACCACGCAGCAAAAACAGATGGCAGAGGTGGAAGAGCAGCAGCGGCTCAAGTACCTGCAATTTGTTCAAGTGGCTGCACTTCACGCCGCGGTTTGCTTCTCAAATGCTTATGGTTATGCCAAGGAGAAATCCGGTCCACTGAGGCCCGGCGTTGAGTCAGCGGAGGGAGCTGTGAAGACTGTTGTTGGACCTGTGTATGACAAGTACCAGGCTCTCCCCGCCAACCTCCTGGCTTTCGTTGATGGCAAGGTCGACGAATCTGTGACCAGGCTGGCTTCATCCCAAGCAATGTCTTTTGCTCAGAAAGCGCCAGAGGCGGCTCGAGCTGTTGCATCGGAGTTTCAGCAAAGGAGCACAAGCATGGCTAAGTCGGTATACTCCAAGTACGAACCGAAAGCTGAGCAATGTGCTGTTTGTGCATGGAGAAAGCTGAATGAGCTACCTGTGGTACCGAAGGTGGCTGAGATTGTTGTACCGACTGCTGCATATTGTTCCGAAAAGTACAACCACAGTGTCCGTTGCACTGCGGAGAAGGGGTACAGGGTGGCCTCCTATTTGCCTTTGGTTCCAATAGAGAAAATTGTAAAGGTTGTTGGAGGCAATGTGCCAGAAGCCGTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

213

Amino Acids

23.24

Weight (kDa)

8.49

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 17 - 204 5.8e-74 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 81
Acc65I GGTACC 1 cut(s) 466
AccB1I GGYRCC 1 cut(s) 466
AccI GTMKAC 2 cut(s) 270, 393
AccII CGCG 1 cut(s) 107
AciI CCGC 7 cut(s) 61, 105, 107, 179, 237, 335, 545
AfaI GTAC 7 cut(s) 71, 224, 404, 468, 492, 521, 557
AgsI TTSAA 1 cut(s) 86
AjnI CCWGG 3 cut(s) 225, 246, 284
AluBI AGCT 5 cut(s) 188, 344, 416, 448, 457
AluI AGCT 5 cut(s) 188, 344, 416, 448, 457
Alw21I GWGCWC 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 339
AoxI GGCC 2 cut(s) 161, 564
ApeKI GCWGC 5 cut(s) 25, 55, 58, 92, 500
Asp700I GAANNNNTTC 1 cut(s) 633
Asp718I GGTACC 1 cut(s) 466
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 3 cut(s) 152, 162, 206
AsuC2I CCSGG 1 cut(s) 165
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 2 cut(s) 152, 206
BanI GGYRCC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 374
BbvI GCAGC 5 cut(s) 37, 67, 70, 79, 487
BccI CCATC 2 cut(s) 31, 254
BceAI ACGGC 1 cut(s) 617
BciT130I CCWGG 3 cut(s) 227, 248, 286
BcnI CCSGG 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 81
BisI GCNGC 8 cut(s) 26, 56, 59, 62, 93, 105, 336, 501
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 8 cut(s) 27, 57, 60, 63, 94, 106, 337, 502
Bme1390I CCNGG 4 cut(s) 165, 227, 248, 286
Bme18I GGWCC 2 cut(s) 152, 206
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 3 cut(s) 152, 162, 206
BmiI GGNNCC 2 cut(s) 468, 585
BmrFI CCNGG 4 cut(s) 165, 227, 248, 286
BmsI GCATC 1 cut(s) 359
BpiI GAAGAC 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 105, 138
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 4 cut(s) 539, 569, 606, 636
Bse3DI GCAATG 3 cut(s) 312, 428, 625
BseBI CCWGG 3 cut(s) 227, 248, 286
BseDI CCNNGG 2 cut(s) 105, 138
BseGI GGATG 1 cut(s) 296
BseMI GCAATG 3 cut(s) 312, 428, 625
BseMII CTCAG 4 cut(s) 149, 332, 408, 471
BseXI GCAGC 5 cut(s) 37, 67, 70, 79, 487
BsgI GTGCAG 1 cut(s) 78
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 163, 566
BshNI GGYRCC 1 cut(s) 466
BsiHKAI GWGCWC 1 cut(s) 374
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 2 cut(s) 150, 165
BsnI GGCC 2 cut(s) 163, 566
BsoBI CYCGRG 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 374
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 7 cut(s) 61, 105, 107, 179, 237, 335, 545
BspANI GGCC 2 cut(s) 163, 566
BspCNI CTCAG 4 cut(s) 150, 331, 409, 472
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 2 cut(s) 468, 585
BspMI ACCTGC 1 cut(s) 81
BspQI GCTCTTC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 466
BsrDI GCAATG 3 cut(s) 312, 428, 625
BssECI CCNNGG 2 cut(s) 105, 138
BssNAI GTATAC 1 cut(s) 394
BssT1I CCWWGG 1 cut(s) 138
Bst1107I GTATAC 1 cut(s) 394
Bst2UI CCWGG 3 cut(s) 227, 248, 286
Bst4CI ACNGT 2 cut(s) 199, 530
Bst6I CTCTTC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 5 cut(s) 158, 318, 384, 417, 480
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 1 cut(s) 296
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstMWI GCNNNNNNNGC 9 cut(s) 61, 101, 185, 236, 323, 332, 341, 434, 454
BstNI CCWGG 3 cut(s) 227, 248, 286
BstSCI CCNGG 4 cut(s) 163, 225, 246, 284
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 5 cut(s) 37, 67, 70, 79, 487
BstV2I GAAGAC 1 cut(s) 200
BstXI CCANNNNNNTGG 1 cut(s) 247
BstZ17I GTATAC 1 cut(s) 394
BsuRI GGCC 2 cut(s) 163, 566
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 1 cut(s) 296
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 3 cut(s) 155, 535, 540
BveI ACCTGC 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 291
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 152, 162, 206
Cfr42I CCGCGG 1 cut(s) 108
Csp6I GTAC 7 cut(s) 70, 223, 403, 467, 491, 520, 556
CviAII CATG 2 cut(s) 379, 438
CviQI GTAC 7 cut(s) 70, 223, 403, 467, 491, 520, 556
DdeI CTNAG 5 cut(s) 158, 318, 384, 417, 480
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 152, 206
Eco88I CYCGRG 1 cut(s) 339
EcoRII CCWGG 3 cut(s) 225, 246, 284
EcoT14I CCWWGG 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 2 cut(s) 382, 441
FaiI YATR 7 cut(s) 129, 135, 216, 380, 394, 439, 505
FatI CATG 2 cut(s) 378, 437
FauI CCCGC 1 cut(s) 244
FblI GTMKAC 2 cut(s) 270, 393
Fnu4HI GCNGC 8 cut(s) 26, 56, 59, 62, 93, 105, 336, 501
FokI GGATG 1 cut(s) 283
Fsp4HI GCNGC 8 cut(s) 26, 56, 59, 62, 93, 105, 336, 501
GlaI GCGC 1 cut(s) 327
GluI GCNGC 8 cut(s) 26, 56, 59, 62, 93, 105, 336, 501
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 2 cut(s) 163, 566
HapII CCGG 2 cut(s) 150, 165
HhaI GCGC 1 cut(s) 328
Hin1II CATG 2 cut(s) 382, 441
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 2 cut(s) 173, 275
HpaII CCGG 2 cut(s) 150, 165
Hpy166II GTNNAC 3 cut(s) 155, 271, 394
Hpy188I TCNGA 3 cut(s) 321, 355, 514
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 3 cut(s) 155, 271, 394
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 2 cut(s) 466, 544
HpyCH4III ACNGT 2 cut(s) 199, 530
HpyCH4V TGCA 6 cut(s) 76, 95, 350, 437, 503, 540
HpyF10VI GCNNNNNNNGC 9 cut(s) 61, 101, 185, 236, 323, 332, 341, 434, 454
HpyF3I CTNAG 5 cut(s) 158, 318, 384, 417, 480
Hsp92II CATG 2 cut(s) 382, 441
HspAI GCGC 1 cut(s) 326
KpnI GGTACC 1 cut(s) 470
KspI CCGCGG 1 cut(s) 108
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 2 cut(s) 185, 369
Lsp1109I GCAGC 5 cut(s) 37, 67, 70, 79, 487
LweI GCATC 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 280
MboII GAAGA 2 cut(s) 62, 205
MhlI GDGCHC 1 cut(s) 374
MluCI AATT 2 cut(s) 77, 597
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 184, 592
MnlI CCTC 8 cut(s) 4, 37, 153, 175, 254, 325, 577, 608
MroXI GAANNNNTTC 1 cut(s) 633
MslI CAYNNNNRTG 1 cut(s) 377
MspA1I CMGCKG 3 cut(s) 61, 107, 179
MspI CCGG 2 cut(s) 150, 165
MspR9I CCNGG 4 cut(s) 165, 227, 248, 286
MvaI CCWGG 3 cut(s) 227, 248, 286
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 9 cut(s) 61, 101, 185, 236, 323, 332, 341, 434, 454
NciI CCSGG 1 cut(s) 165
NlaIII CATG 2 cut(s) 382, 441
NlaIV GGNNCC 2 cut(s) 468, 585
NmuCI GTSAC 1 cut(s) 280
PaeR7I CTCGAG 1 cut(s) 339
PciSI GCTCTTC 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 8 cut(s) 27, 57, 60, 63, 94, 106, 337, 502
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp6I CCWGG 3 cut(s) 225, 246, 284
PspGI CCWGG 3 cut(s) 225, 246, 284
PspN4I GGNNCC 2 cut(s) 468, 585
PspPI GGNCC 3 cut(s) 152, 162, 206
PspXI VCTCGAGB 1 cut(s) 339
RsaI GTAC 7 cut(s) 71, 224, 404, 468, 492, 521, 557
RsaNI GTAC 7 cut(s) 70, 223, 403, 467, 491, 520, 556
RseI CAYNNNNRTG 1 cut(s) 377
SacII CCGCGG 1 cut(s) 108
SalI GTCGAC 1 cut(s) 269
SapI GCTCTTC 1 cut(s) 45
SatI GCNGC 8 cut(s) 26, 56, 59, 62, 93, 105, 336, 501
Sau96I GGNCC 3 cut(s) 152, 162, 206
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 4 cut(s) 165, 227, 248, 286
SduI GDGCHC 1 cut(s) 374
SfaNI GCATC 1 cut(s) 359
Sfr274I CTCGAG 1 cut(s) 339
Sfr303I CCGCGG 1 cut(s) 108
SgrBI CCGCGG 1 cut(s) 108
SinI GGWCC 2 cut(s) 152, 206
SlaI CTCGAG 1 cut(s) 339
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 3 cut(s) 6, 65, 339
SmoI CTYRAG 3 cut(s) 6, 65, 339
Sse9I AATT 2 cut(s) 77, 597
SsiI CCGC 7 cut(s) 61, 105, 107, 179, 237, 335, 545
StyD4I CCNGG 4 cut(s) 163, 225, 246, 284
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 2 cut(s) 199, 530
TaqI TCGA 2 cut(s) 270, 340
TasI AATT 2 cut(s) 77, 597
TatI WGTACW 1 cut(s) 519
TauI GCSGC 3 cut(s) 64, 107, 338
TfiI GAWTC 1 cut(s) 275
TscAI CASTG 3 cut(s) 162, 535, 547
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 5 cut(s) 25, 55, 58, 92, 500
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 285
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 3 cut(s) 162, 535, 547
VpaK11BI GGWCC 2 cut(s) 152, 206
XhoI CTCGAG 1 cut(s) 339
XmiI GTMKAC 2 cut(s) 270, 393
XmnI GAANNNNTTC 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.