Rh2DG302800

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002647
Physical Location & Seq
Reverse (-)
36208113 .. 36208757
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG302800.1

Sequence Viewer

Length: 645 bp
ATGGCTCAAGAGGACTTGACCACGCAGCAAAAACAGATGGCGGAGGAGGTGGAAGAGCAGCAGCGGCTCAAGTACCTGCAATTTGTTCAAGTGGCTGCACTTCACGCCGCGGTTTGCTTCTCAAATGCTTATGGTTATGCCAAGGAGAAATCCGGTCCACTGAGGCCCGGCGTTGAGTCAGCGGAGGGAGCTGTGAAGACTGTTGTTGGACCTGTGTATGACAAGTACCAGGCTCTCCCCGCCAACCTCCTTGCTTTCGTTGATGGCAAGGTCGACGAATCTGTGACCAAGCTGGCTTCATCCCAAGCAATGTCTTTTGCTCAGAAAGCGCCAGAGGCGGCTCGAGCTGTTGCATTGGAGTTTCAGCAAAGGAGCACAAGCATGGCTAAGTTGGTATACTCCAAGTACGAACCGAAAGCTGAGCAATGTGCTGTTTGTGCATGGAGAAAGCTGAATGAGCTACCTGTGGTACCGAAGGTGGCTGAGATTGTTGTACCGACTGCTGCATATTGTTCCGAAAAGTACAATCACAGTGTCCGTTGCACTGCGGAGAAGGGGTACAGGGTGGCCTCCTATTTGCCTTTGGTTCCAACAGAAAAAATTGCTAAGGTTTTTGGAGGGAATGTGCCTGAACCCGTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

214

Amino Acids

23.45

Weight (kDa)

8.49

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 18 - 206 4.9e-74 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 84
Acc65I GGTACC 1 cut(s) 469
AccB1I GGYRCC 1 cut(s) 469
AccI GTMKAC 2 cut(s) 273, 396
AccII CGCG 1 cut(s) 110
AciI CCGC 8 cut(s) 41, 64, 108, 110, 182, 240, 338, 548
AfaI GTAC 7 cut(s) 74, 227, 407, 471, 495, 524, 560
AgsI TTSAA 1 cut(s) 89
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 6 cut(s) 191, 292, 347, 419, 451, 460
AluI AGCT 6 cut(s) 191, 292, 347, 419, 451, 460
Alw21I GWGCWC 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 342
AoxI GGCC 2 cut(s) 164, 567
ApeKI GCWGC 5 cut(s) 25, 58, 61, 95, 503
Asp700I GAANNNNTTC 1 cut(s) 636
Asp718I GGTACC 1 cut(s) 469
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 3 cut(s) 155, 165, 209
AsuC2I CCSGG 1 cut(s) 168
AvaI CYCGRG 1 cut(s) 342
AvaII GGWCC 2 cut(s) 155, 209
BanI GGYRCC 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 5 cut(s) 37, 70, 73, 82, 490
BccI CCATC 2 cut(s) 31, 257
BciT130I CCWGG 1 cut(s) 230
BcnI CCSGG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 332
BfuAI ACCTGC 1 cut(s) 84
BisI GCNGC 8 cut(s) 26, 59, 62, 65, 96, 108, 339, 504
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 8 cut(s) 27, 60, 63, 66, 97, 109, 340, 505
Bme1390I CCNGG 2 cut(s) 168, 230
Bme18I GGWCC 2 cut(s) 155, 209
BmeT110I CYCGRG 1 cut(s) 342
BmgT120I GGNCC 3 cut(s) 155, 165, 209
BmiI GGNNCC 2 cut(s) 471, 588
BmrFI CCNGG 2 cut(s) 168, 230
BpiI GAAGAC 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 108, 141
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 4 cut(s) 542, 572, 609, 639
Bse3DI GCAATG 2 cut(s) 315, 431
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 108, 141
BseGI GGATG 1 cut(s) 299
BseMI GCAATG 2 cut(s) 315, 431
BseMII CTCAG 4 cut(s) 152, 335, 411, 474
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 5 cut(s) 37, 70, 73, 82, 490
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 2 cut(s) 166, 569
BshNI GGYRCC 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 377
BsiHKCI CYCGRG 1 cut(s) 342
BsiSI CCGG 2 cut(s) 153, 168
BsnI GGCC 2 cut(s) 166, 569
BsoBI CYCGRG 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 377
Bsp1720I GCTNAGC 1 cut(s) 420
BspACI CCGC 8 cut(s) 41, 64, 108, 110, 182, 240, 338, 548
BspANI GGCC 2 cut(s) 166, 569
BspCNI CTCAG 4 cut(s) 153, 334, 412, 475
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 2 cut(s) 471, 588
BspMI ACCTGC 1 cut(s) 84
BspQI GCTCTTC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 469
BsrDI GCAATG 2 cut(s) 315, 431
BssECI CCNNGG 2 cut(s) 108, 141
BssNAI GTATAC 1 cut(s) 397
BssT1I CCWWGG 1 cut(s) 141
Bst1107I GTATAC 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 2 cut(s) 202, 533
Bst6I CTCTTC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 6 cut(s) 161, 321, 387, 420, 483, 606
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 299
BstFNI CGCG 1 cut(s) 110
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 1 cut(s) 331
BstMWI GCNNNNNNNGC 9 cut(s) 64, 104, 188, 239, 326, 335, 344, 437, 457
BstNI CCWGG 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 166, 228
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 5 cut(s) 37, 70, 73, 82, 490
BstV2I GAAGAC 1 cut(s) 203
BstZ17I GTATAC 1 cut(s) 397
BsuRI GGCC 2 cut(s) 166, 569
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 299
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 3 cut(s) 158, 538, 543
BveI ACCTGC 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 294
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 3 cut(s) 155, 165, 209
Cfr42I CCGCGG 1 cut(s) 111
Csp6I GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
CviAII CATG 2 cut(s) 382, 441
CviQI GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
DdeI CTNAG 6 cut(s) 161, 321, 387, 420, 483, 606
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
EciI GGCGGA 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 2 cut(s) 155, 209
Eco88I CYCGRG 1 cut(s) 342
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 2 cut(s) 385, 444
FaiI YATR 7 cut(s) 132, 138, 219, 383, 397, 442, 508
FatI CATG 2 cut(s) 381, 440
FauI CCCGC 1 cut(s) 247
FblI GTMKAC 2 cut(s) 273, 396
Fnu4HI GCNGC 8 cut(s) 26, 59, 62, 65, 96, 108, 339, 504
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 8 cut(s) 26, 59, 62, 65, 96, 108, 339, 504
GlaI GCGC 1 cut(s) 330
GluI GCNGC 8 cut(s) 26, 59, 62, 65, 96, 108, 339, 504
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 2 cut(s) 166, 569
HapII CCGG 2 cut(s) 153, 168
HhaI GCGC 1 cut(s) 331
Hin1II CATG 2 cut(s) 385, 444
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 2 cut(s) 176, 278
HpaII CCGG 2 cut(s) 153, 168
Hpy166II GTNNAC 3 cut(s) 158, 274, 397
Hpy188I TCNGA 2 cut(s) 324, 517
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 3 cut(s) 158, 274, 397
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 469, 547
HpyCH4III ACNGT 2 cut(s) 202, 533
HpyCH4V TGCA 6 cut(s) 79, 98, 353, 440, 506, 543
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 104, 188, 239, 326, 335, 344, 437, 457
HpyF3I CTNAG 6 cut(s) 161, 321, 387, 420, 483, 606
Hsp92II CATG 2 cut(s) 385, 444
HspAI GCGC 1 cut(s) 329
KpnI GGTACC 1 cut(s) 473
KspI CCGCGG 1 cut(s) 111
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 2 cut(s) 188, 372
Lsp1109I GCAGC 5 cut(s) 37, 70, 73, 82, 490
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 2 cut(s) 65, 208
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 2 cut(s) 80, 600
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 2 cut(s) 187, 614
MnlI CCTC 9 cut(s) 4, 37, 40, 156, 178, 257, 328, 580, 611
MroXI GAANNNNTTC 1 cut(s) 636
MslI CAYNNNNRTG 1 cut(s) 380
MspA1I CMGCKG 3 cut(s) 64, 110, 182
MspI CCGG 2 cut(s) 153, 168
MspR9I CCNGG 2 cut(s) 168, 230
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 9 cut(s) 64, 104, 188, 239, 326, 335, 344, 437, 457
NciI CCSGG 1 cut(s) 168
NlaIII CATG 2 cut(s) 385, 444
NlaIV GGNNCC 2 cut(s) 471, 588
NmuCI GTSAC 1 cut(s) 283
PaeR7I CTCGAG 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 8 cut(s) 27, 60, 63, 66, 97, 109, 340, 505
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 471, 588
PspPI GGNCC 3 cut(s) 155, 165, 209
PspXI VCTCGAGB 1 cut(s) 342
RsaI GTAC 7 cut(s) 74, 227, 407, 471, 495, 524, 560
RsaNI GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
RseI CAYNNNNRTG 1 cut(s) 380
SacII CCGCGG 1 cut(s) 111
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 48
SatI GCNGC 8 cut(s) 26, 59, 62, 65, 96, 108, 339, 504
Sau96I GGNCC 3 cut(s) 155, 165, 209
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 2 cut(s) 168, 230
SduI GDGCHC 1 cut(s) 377
Sfr274I CTCGAG 1 cut(s) 342
Sfr303I CCGCGG 1 cut(s) 111
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 2 cut(s) 155, 209
SlaI CTCGAG 1 cut(s) 342
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 3 cut(s) 6, 68, 342
SmoI CTYRAG 3 cut(s) 6, 68, 342
Sse9I AATT 2 cut(s) 80, 600
SsiI CCGC 8 cut(s) 41, 64, 108, 110, 182, 240, 338, 548
StyD4I CCNGG 2 cut(s) 166, 228
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 202, 533
TaqI TCGA 2 cut(s) 273, 343
TasI AATT 2 cut(s) 80, 600
TatI WGTACW 1 cut(s) 522
TauI GCSGC 3 cut(s) 67, 110, 341
TfiI GAWTC 1 cut(s) 278
TscAI CASTG 3 cut(s) 165, 538, 550
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 5 cut(s) 25, 58, 61, 95, 503
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 288
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 3 cut(s) 165, 538, 550
VpaK11BI GGWCC 2 cut(s) 155, 209
XhoI CTCGAG 1 cut(s) 342
XmiI GTMKAC 2 cut(s) 273, 396
XmnI GAANNNNTTC 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.