Rw2G022020

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002647
Physical Location & Seq
Reverse (-)
30789572 .. 30791251
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022020.1

Sequence Viewer

Length: 891 bp
ATGACTCCGCTCACAGAACCTCACTGGAACCCGGCACAGCAGCCGGAGATGGCCGAGGACGGCAGTTCAATAGTTGCAGAGTTGGACTTTGCGGCAGAGGGATTGTCATCCAGAGAAGCGGAGCTAAGGCAAGAGATTGAGCAACACAAGCACGAAAAGGCGGTGCCGGAGGAGAAGCACCGATTCGAGATTCTAGAGAGGCTGAAGTTCCAACAGAAAGAAACTCCTTTGTTAGAAGGTAGATATATGGCTCAAGAGGACTTGACGACGCAGCAAAACCAGATGGCGGAGGAGGTGGAAGAGCAGCAGCGGCTCAAGTACCTGCAATTTGTTCAAGTGGCTGCACTTCACGCCGCGGTTTGCTTCTCAAATGCTTATGGTTATGCCAAGGAGAAATCCGGTCCACTGAGGCCCGGCGTTGAGTCAGCGGAGGGAGCTGTGAAGACTGTTGTTGGACCTGTGTATGACACGTACCAGGCTCTCCCCGCCAACCTCCTGGCTTTCGTTGATGGCAAGGTCGACGAATCTGTGACCAGGCTGGCTTCATCCCAACCAATGTCTTTTGCTCAGAAAGCGCCAGAGGCGGCTCGAGCTGCTGCATCGGAGTTTCAGCAAAGGAGCACAAGCATGGCTAAATCGGTATACTCCAAGTACGAACCGAAAGCTGAGCAATGTGCTGTTTGTGCATGGAGAAAACTGAATGAGCTACCTGTGGTACCGAAGGTGGCTGAGATTGTTGTACCGACTGCTGCATATTGTTCCGAAAAGTACAACCACAGTGTCCGTTGCACTGCGGAGAAGGGGTACAGGGTGGCCTCCTATTTGCCTTTGGTTCCAACAGAAAAAATTGCTAAGGTTTTTGGAGGGAATGTGCCTGAACCCGTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

296

Amino Acids

32.93

Weight (kDa)

5.56

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 100 - 288 2.2e-72 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 330
Acc65I GGTACC 1 cut(s) 715
AccB1I GGYRCC 2 cut(s) 163, 715
AccBSI CCGCTC 1 cut(s) 10
AccI GTMKAC 2 cut(s) 519, 642
AccII CGCG 1 cut(s) 356
AcoI YGGCCR 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 7 cut(s) 320, 473, 653, 717, 741, 770, 806
AfiI CCNNNNNNNGG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 2 cut(s) 69, 335
AjnI CCWGG 3 cut(s) 474, 495, 533
AluBI AGCT 5 cut(s) 124, 437, 593, 665, 706
AluI AGCT 5 cut(s) 124, 437, 593, 665, 706
Alw21I GWGCWC 1 cut(s) 623
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 3 cut(s) 51, 410, 813
ApeKI GCWGC 8 cut(s) 40, 271, 304, 307, 341, 593, 596, 749
Asp700I GAANNNNTTC 1 cut(s) 882
Asp718I GGTACC 1 cut(s) 715
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 3 cut(s) 401, 411, 455
AsuC2I CCSGG 2 cut(s) 32, 414
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 2 cut(s) 401, 455
BanI GGYRCC 2 cut(s) 163, 715
BbsI GAAGAC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 623
BbvI GCAGC 8 cut(s) 52, 283, 316, 319, 328, 580, 583, 736
BccI CCATC 3 cut(s) 43, 277, 503
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 3 cut(s) 476, 497, 535
BcnI CCSGG 2 cut(s) 32, 414
BfaI CTAG 1 cut(s) 194
BfoI RGCGCY 1 cut(s) 578
BfuAI ACCTGC 1 cut(s) 330
BlpI GCTNAGC 1 cut(s) 666
Bme1390I CCNGG 5 cut(s) 32, 414, 476, 497, 535
Bme18I GGWCC 2 cut(s) 401, 455
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 3 cut(s) 401, 411, 455
BmiI GGNNCC 4 cut(s) 29, 165, 717, 834
BmrFI CCNGG 5 cut(s) 32, 414, 476, 497, 535
BmsI GCATC 1 cut(s) 608
BpiI GAAGAC 1 cut(s) 449
Bpu10I CCTNAGC 2 cut(s) 125, 852
Bpu1102I GCTNAGC 1 cut(s) 666
BpuEI CTTGAG 2 cut(s) 237, 299
BpuMI CCSGG 2 cut(s) 32, 414
BsaAI YACGTR 1 cut(s) 471
BsaBI GATNNNNATC 1 cut(s) 106
BsaJI CCNNGG 3 cut(s) 54, 354, 387
BsaWI WCCGGW 1 cut(s) 398
BsaXI ACNNNNNCTCC 4 cut(s) 788, 818, 855, 885
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse1I ACTGG 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 677
Bse8I GATNNNNATC 1 cut(s) 106
BseBI CCWGG 3 cut(s) 476, 497, 535
BseDI CCNNGG 3 cut(s) 54, 354, 387
BseGI GGATG 2 cut(s) 107, 545
BseJI GATNNNNATC 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 286
BseMI GCAATG 1 cut(s) 677
BseMII CTCAG 4 cut(s) 398, 581, 657, 720
BseNI ACTGG 1 cut(s) 29
BseRI GAGGAG 2 cut(s) 185, 305
BseXI GCAGC 8 cut(s) 52, 283, 316, 319, 328, 580, 583, 736
BsgI GTGCAG 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 356
BshFI GGCC 3 cut(s) 53, 412, 815
BshNI GGYRCC 2 cut(s) 163, 715
BsiHKAI GWGCWC 1 cut(s) 623
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 5 cut(s) 32, 44, 167, 399, 414
BslI CCNNNNNNNGG 1 cut(s) 286
BsnI GGCC 3 cut(s) 53, 412, 815
BsoBI CYCGRG 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 623
Bsp1720I GCTNAGC 1 cut(s) 666
BspANI GGCC 3 cut(s) 53, 412, 815
BspCNI CTCAG 4 cut(s) 399, 580, 658, 721
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 4 cut(s) 29, 165, 717, 834
BspMI ACCTGC 1 cut(s) 330
BspQI GCTCTTC 1 cut(s) 294
BspT107I GGYRCC 2 cut(s) 163, 715
BsrBI CCGCTC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 677
BsrI ACTGG 1 cut(s) 29
BssECI CCNNGG 3 cut(s) 54, 354, 387
BssNAI GTATAC 1 cut(s) 643
BssT1I CCWWGG 1 cut(s) 387
Bst1107I GTATAC 1 cut(s) 643
Bst2UI CCWGG 3 cut(s) 476, 497, 535
Bst4CI ACNGT 2 cut(s) 448, 779
Bst6I CTCTTC 1 cut(s) 294
BstBAI YACGTR 1 cut(s) 471
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 6 cut(s) 125, 407, 567, 666, 729, 852
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 107, 545
BstFNI CGCG 1 cut(s) 356
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 1 cut(s) 577
BstNI CCWGG 3 cut(s) 476, 497, 535
BstSCI CCNGG 5 cut(s) 30, 412, 474, 495, 533
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 8 cut(s) 52, 283, 316, 319, 328, 580, 583, 736
BstV2I GAAGAC 1 cut(s) 449
BstXI CCANNNNNNTGG 1 cut(s) 496
BstZ17I GTATAC 1 cut(s) 643
BsuRI GGCC 3 cut(s) 53, 412, 815
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 2 cut(s) 107, 545
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 4 cut(s) 22, 404, 784, 789
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 540
CfoI GCGC 1 cut(s) 577
Cfr13I GGNCC 3 cut(s) 401, 411, 455
Cfr42I CCGCGG 1 cut(s) 357
CseI GACGC 1 cut(s) 277
Csp6I GTAC 7 cut(s) 319, 472, 652, 716, 740, 769, 805
CviAII CATG 2 cut(s) 628, 687
CviQI GTAC 7 cut(s) 319, 472, 652, 716, 740, 769, 805
DdeI CTNAG 6 cut(s) 125, 407, 567, 666, 729, 852
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
EciI GGCGGA 1 cut(s) 302
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 401, 455
Eco57I CTGAAG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 588
EcoRII CCWGG 3 cut(s) 474, 495, 533
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 2 cut(s) 631, 690
FaiI YATR 9 cut(s) 246, 248, 378, 384, 465, 629, 643, 688, 754
FatI CATG 2 cut(s) 627, 686
FauI CCCGC 1 cut(s) 493
FblI GTMKAC 2 cut(s) 519, 642
FokI GGATG 2 cut(s) 94, 532
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 576
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 3 cut(s) 53, 412, 815
HapII CCGG 5 cut(s) 32, 44, 167, 399, 414
HgaI GACGC 1 cut(s) 277
HhaI GCGC 1 cut(s) 577
Hin1II CATG 2 cut(s) 631, 690
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 5 cut(s) 4, 183, 190, 422, 524
HpaII CCGG 5 cut(s) 32, 44, 167, 399, 414
Hpy166II GTNNAC 3 cut(s) 404, 520, 643
Hpy188I TCNGA 3 cut(s) 570, 604, 763
Hpy188III TCNNGA 4 cut(s) 111, 187, 194, 254
Hpy8I GTNNAC 3 cut(s) 404, 520, 643
Hpy99I CGWCG 2 cut(s) 271, 524
HpyAV CCTTC 3 cut(s) 230, 715, 793
HpyCH4III ACNGT 2 cut(s) 448, 779
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 7 cut(s) 77, 325, 344, 599, 686, 752, 789
HpyF3I CTNAG 6 cut(s) 125, 407, 567, 666, 729, 852
HpySE526I ACGT 1 cut(s) 470
Hsp92II CATG 2 cut(s) 631, 690
HspAI GCGC 1 cut(s) 575
KpnI GGTACC 1 cut(s) 719
KspI CCGCGG 1 cut(s) 357
LguI GCTCTTC 1 cut(s) 294
LmnI GCTCC 3 cut(s) 121, 434, 618
Lsp1109I GCAGC 8 cut(s) 52, 283, 316, 319, 328, 580, 583, 736
LweI GCATC 1 cut(s) 608
MaeI CTAG 1 cut(s) 194
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 1 cut(s) 529
MbiI CCGCTC 1 cut(s) 10
MboII GAAGA 2 cut(s) 311, 454
MhlI GDGCHC 1 cut(s) 623
MluCI AATT 2 cut(s) 326, 846
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 4 cut(s) 63, 235, 433, 860
MroXI GAANNNNTTC 1 cut(s) 882
MslI CAYNNNNRTG 1 cut(s) 626
MspA1I CMGCKG 3 cut(s) 310, 356, 428
MspI CCGG 5 cut(s) 32, 44, 167, 399, 414
MspR9I CCNGG 5 cut(s) 32, 414, 476, 497, 535
MvaI CCWGG 3 cut(s) 476, 497, 535
MvnI CGCG 1 cut(s) 356
NciI CCSGG 2 cut(s) 32, 414
NlaIII CATG 2 cut(s) 631, 690
NlaIV GGNNCC 4 cut(s) 29, 165, 717, 834
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 529
PaeR7I CTCGAG 1 cut(s) 588
PciSI GCTCTTC 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 3 cut(s) 183, 190, 524
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 471
Psp6I CCWGG 3 cut(s) 474, 495, 533
PspGI CCWGG 3 cut(s) 474, 495, 533
PspN4I GGNNCC 4 cut(s) 29, 165, 717, 834
PspPI GGNCC 3 cut(s) 401, 411, 455
PspXI VCTCGAGB 1 cut(s) 588
RsaI GTAC 7 cut(s) 320, 473, 653, 717, 741, 770, 806
RsaNI GTAC 7 cut(s) 319, 472, 652, 716, 740, 769, 805
RseI CAYNNNNRTG 1 cut(s) 626
SacII CCGCGG 1 cut(s) 357
SalI GTCGAC 1 cut(s) 518
SapI GCTCTTC 1 cut(s) 294
Sau96I GGNCC 3 cut(s) 401, 411, 455
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 5 cut(s) 32, 414, 476, 497, 535
SduI GDGCHC 1 cut(s) 623
SfaNI GCATC 1 cut(s) 608
Sfr274I CTCGAG 1 cut(s) 588
Sfr303I CCGCGG 1 cut(s) 357
SgrBI CCGCGG 1 cut(s) 357
SinI GGWCC 2 cut(s) 401, 455
SlaI CTCGAG 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 626
SmlI CTYRAG 3 cut(s) 252, 314, 588
SmoI CTYRAG 3 cut(s) 252, 314, 588
Sse9I AATT 2 cut(s) 326, 846
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 5 cut(s) 30, 412, 474, 495, 533
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 448, 779
TaiI ACGT 1 cut(s) 473
TaqI TCGA 3 cut(s) 186, 519, 589
TasI AATT 2 cut(s) 326, 846
TatI WGTACW 1 cut(s) 768
TauI GCSGC 4 cut(s) 95, 313, 356, 587
TfiI GAWTC 3 cut(s) 183, 190, 524
TscAI CASTG 4 cut(s) 29, 411, 784, 796
TseFI GTSAC 1 cut(s) 529
TseI GCWGC 8 cut(s) 40, 271, 304, 307, 341, 593, 596, 749
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 1 cut(s) 534
TspGWI ACGGA 1 cut(s) 773
TspRI CASTG 4 cut(s) 29, 411, 784, 796
VpaK11BI GGWCC 2 cut(s) 401, 455
XbaI TCTAGA 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 588
XmiI GTMKAC 2 cut(s) 519, 642
XmnI GAANNNNTTC 1 cut(s) 882
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.