RLG00000018432

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002647
Physical Location & Seq
Reverse (-)
29708473 .. 29709117
645 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018432

Sequence Viewer

Length: 645 bp
ATGGCTCAAGAGGACTTGACCACGCAGCAAAAACAGATGGCGGAGGAGGTGGAAGAGCAGCAGCGGCTCAAGTACCTGCAATTTGTTCAAGTGGCTGCACTTCACGCCGCGGTTTGCTTCTCGAATGCTTATGGTTATGCCAAGGAGAAATCCGGCCCTCTGAGGCCCCGCGTTGAGTCAGCGGAGGGAGCTGTGAAGACCGTTGTTGGACCTGTGTATGAGAAGTACCAGGCTCTCCCCGCCAACCTCCTCGCTTTCGTTGATAGCAAGGTCGACGAATCTGTGACCAAGCTGGCATCATCCCAAGCAATATCTGCTGCATTGAACGCCCCAGAGGCGGCTCGAGCTGTAGCATCGGAGGTTCAGCAAAGGAGCACAAGCATGGCTAAGTCAGTATACTCCAAGTACGAACCGAAAGCTGAGCAATGTGCTGTTTGTGCTTGGAGAAAGCTGAATAAGCTACCTGTAGTACCAAAGGTGGCTGAGATTGTTGTACCGACTGCTGCATATTGTTCCGAAAAGTACAACAACAGTGTCCGTTGCACTGCGGAGAAGGGGTACAGGGTGGCCTCCTATTTGCCTTTGGTTCCAACAGAGAAAATTGCTAAGGTTTTTGGAGGGAATGTGCCTGAAGCCGTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

215

Amino Acids

23.32

Weight (kDa)

8.85

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 18 - 206 9.8e-74 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 84
AccI GTMKAC 2 cut(s) 273, 396
AccII CGCG 2 cut(s) 110, 171
AciI CCGC 9 cut(s) 41, 64, 108, 110, 169, 182, 240, 338, 548
AfaI GTAC 7 cut(s) 74, 227, 407, 471, 495, 524, 560
AfiI CCNNNNNNNGG 1 cut(s) 337
AgsI TTSAA 2 cut(s) 89, 325
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 6 cut(s) 191, 292, 347, 419, 451, 460
AluI AGCT 6 cut(s) 191, 292, 347, 419, 451, 460
Alw21I GWGCWC 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 342
AoxI GGCC 3 cut(s) 154, 164, 567
ApeKI GCWGC 6 cut(s) 25, 58, 61, 95, 317, 503
Asp700I GAANNNNTTC 1 cut(s) 636
AspS9I GGNCC 3 cut(s) 155, 165, 209
AvaI CYCGRG 1 cut(s) 342
AvaII GGWCC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 6 cut(s) 37, 70, 73, 82, 304, 490
BccI CCATC 1 cut(s) 31
BceAI ACGGC 1 cut(s) 620
BciT130I CCWGG 1 cut(s) 230
BfmI CTRYAG 2 cut(s) 348, 465
BfuAI ACCTGC 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 335
BisI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 9 cut(s) 27, 60, 63, 66, 97, 109, 319, 340, 505
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 342
BmgT120I GGNCC 3 cut(s) 155, 165, 209
BmiI GGNNCC 2 cut(s) 167, 588
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 2 cut(s) 305, 362
BpiI GAAGAC 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 108, 141
BsaXI ACNNNNNCTCC 4 cut(s) 542, 572, 609, 639
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 431
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 108, 141
BseGI GGATG 1 cut(s) 299
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 431
BseMII CTCAG 3 cut(s) 152, 411, 474
BseRI GAGGAG 2 cut(s) 59, 239
BseXI GCAGC 6 cut(s) 37, 70, 73, 82, 304, 490
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 2 cut(s) 110, 171
BshFI GGCC 3 cut(s) 156, 166, 569
BsiHKAI GWGCWC 1 cut(s) 377
BsiHKCI CYCGRG 1 cut(s) 342
BsiSI CCGG 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 337
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 3 cut(s) 156, 166, 569
BsoBI CYCGRG 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 377
Bsp1720I GCTNAGC 1 cut(s) 420
BspACI CCGC 9 cut(s) 41, 64, 108, 110, 169, 182, 240, 338, 548
BspANI GGCC 3 cut(s) 156, 166, 569
BspCNI CTCAG 3 cut(s) 153, 412, 475
BspFNI CGCG 2 cut(s) 110, 171
BspLI GGNNCC 2 cut(s) 167, 588
BspMI ACCTGC 1 cut(s) 84
BspQI GCTCTTC 1 cut(s) 48
BsrDI GCAATG 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 108, 141
BssNAI GTATAC 1 cut(s) 397
BssT1I CCWWGG 1 cut(s) 141
Bst1107I GTATAC 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 2 cut(s) 202, 533
Bst6I CTCTTC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 5 cut(s) 161, 387, 420, 483, 606
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 299
BstFNI CGCG 2 cut(s) 110, 171
BstNI CCWGG 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 228
BstSFI CTRYAG 2 cut(s) 348, 465
BstUI CGCG 2 cut(s) 110, 171
BstV1I GCAGC 6 cut(s) 37, 70, 73, 82, 304, 490
BstV2I GAAGAC 1 cut(s) 203
BstZ17I GTATAC 1 cut(s) 397
BsuRI GGCC 3 cut(s) 156, 166, 569
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 299
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 2 cut(s) 538, 543
BveI ACCTGC 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 294
Cfr13I GGNCC 3 cut(s) 155, 165, 209
Cfr42I CCGCGG 1 cut(s) 111
Csp6I GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
CviAII CATG 1 cut(s) 382
CviQI GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
DdeI CTNAG 5 cut(s) 161, 387, 420, 483, 606
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
EciI GGCGGA 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 1 cut(s) 385
FaiI YATR 6 cut(s) 132, 138, 219, 383, 397, 508
FatI CATG 1 cut(s) 381
FauI CCCGC 2 cut(s) 176, 247
FblI GTMKAC 2 cut(s) 273, 396
Fnu4HI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
GluI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
HaeIII GGCC 3 cut(s) 156, 166, 569
HapII CCGG 1 cut(s) 153
Hin1II CATG 1 cut(s) 385
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 2 cut(s) 176, 278
HpaII CCGG 1 cut(s) 153
Hpy166II GTNNAC 2 cut(s) 274, 397
Hpy188I TCNGA 3 cut(s) 162, 358, 517
Hpy188III TCNNGA 2 cut(s) 8, 121
Hpy8I GTNNAC 2 cut(s) 274, 397
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 1 cut(s) 547
HpyCH4III ACNGT 2 cut(s) 202, 533
HpyCH4V TGCA 5 cut(s) 79, 98, 320, 506, 543
HpyF3I CTNAG 5 cut(s) 161, 387, 420, 483, 606
Hsp92II CATG 1 cut(s) 385
KspI CCGCGG 1 cut(s) 111
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 2 cut(s) 188, 372
LpnPI CCDG 9 cut(s) 89, 166, 215, 225, 242, 278, 345, 477, 547
Lsp1109I GCAGC 6 cut(s) 37, 70, 73, 82, 304, 490
LweI GCATC 2 cut(s) 305, 362
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 2 cut(s) 65, 208
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 2 cut(s) 80, 600
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 2 cut(s) 187, 614
MroXI GAANNNNTTC 1 cut(s) 636
MslI CAYNNNNRTG 1 cut(s) 380
MspA1I CMGCKG 3 cut(s) 64, 110, 182
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 1 cut(s) 230
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 2 cut(s) 110, 171
NlaIII CATG 1 cut(s) 385
NlaIV GGNNCC 2 cut(s) 167, 588
NmuCI GTSAC 1 cut(s) 283
PaeR7I CTCGAG 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 1 cut(s) 130
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 9 cut(s) 27, 60, 63, 66, 97, 109, 319, 340, 505
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 167, 588
PspPI GGNCC 3 cut(s) 155, 165, 209
PspXI VCTCGAGB 1 cut(s) 342
RsaI GTAC 7 cut(s) 74, 227, 407, 471, 495, 524, 560
RsaNI GTAC 7 cut(s) 73, 226, 406, 470, 494, 523, 559
RseI CAYNNNNRTG 1 cut(s) 380
SacII CCGCGG 1 cut(s) 111
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 48
SatI GCNGC 9 cut(s) 26, 59, 62, 65, 96, 108, 318, 339, 504
Sau96I GGNCC 3 cut(s) 155, 165, 209
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 1 cut(s) 377
SfaNI GCATC 2 cut(s) 305, 362
SfcI CTRYAG 2 cut(s) 348, 465
Sfr274I CTCGAG 1 cut(s) 342
Sfr303I CCGCGG 1 cut(s) 111
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 342
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 3 cut(s) 6, 68, 342
SmoI CTYRAG 3 cut(s) 6, 68, 342
Sse9I AATT 2 cut(s) 80, 600
SsiI CCGC 9 cut(s) 41, 64, 108, 110, 169, 182, 240, 338, 548
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 202, 533
TaqI TCGA 3 cut(s) 122, 273, 343
TasI AATT 2 cut(s) 80, 600
TatI WGTACW 1 cut(s) 522
TauI GCSGC 3 cut(s) 67, 110, 341
TfiI GAWTC 1 cut(s) 278
TscAI CASTG 2 cut(s) 538, 550
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 6 cut(s) 25, 58, 61, 95, 317, 503
Tsp45I GTSAC 1 cut(s) 283
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 2 cut(s) 538, 550
VpaK11BI GGWCC 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 342
XmiI GTMKAC 2 cut(s) 273, 396
XmnI GAANNNNTTC 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.