MD04G1201000.v1.1

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002647
Physical Location & Seq
Reverse (-)
28726838 .. 28729005
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1201000.v1.1.491

Sequence Viewer

Length: 753 bp
ATGGCCCAGGAAGACTTGAGCGTGCCGGTGCAGCAACAGATGACCGAGGAGGAGGAACAGAGGCTCAAGTACCTCCAATTTGTTCAAGTGGCTGCTGCTCATGCCGCGGTTTGCTTCATAAACCTGTACGGTTTTGCCAAGGAAAGGTCAGGGCCTTTAAAGCCTGGTGTTGAGTCCGTGGAAGGAACGGTGAAGACCGTTGTTGGACCTGTCTACAACAAGTACCATGATGTCCCCGCTCACCTCCTCACCTTTGTGGATCGCAAGGTTGATCAATCTGTTACCAAGCTGGACAGCCGTGTGCCACCACTGGTTAAGCAAGCCTCATCCAAAGCTCTATCCGCCGCTCAAAAGGCTCCAGAGGCAGCACGAGCGGTTGCCTCTGAGCTTAAGCGTGCCGGGGTTGTAGACACTGCCTCGAGCTGTGCTAAATCTGTCTACTCCAAGTGTGAACCCGCAGCTAAAGACCTGTATGCCAAGTACGAACCACAAGCCGAGCAATATGCTGTTTCTGCTTGGCGAAAGGTGAACCAAGTCCTCCCGAAGGTGGTTGATGTTGTTGTACCAACCGCAGCGTATTGTACAGAAAAGTACAACCAGACCGTGCAGAGTACTGCGGAGAAGGGTTACAGGGTCTCCTACTATCTGCCTTTGGTTCCAACAGAGAAAATTGCCAAGGTTTTCGCCGAGAAGGCTCCCGAAGCAGAGCCTTTGCTTGCAAGTCACGGTGAAACTGAAGTCGCCACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

251

Amino Acids

27.31

Weight (kDa)

7.63

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 16 - 229 1.9e-88 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 239, 347, 374
AccI GTMKAC 3 cut(s) 213, 408, 438
AccII CGCG 1 cut(s) 107
AciI CCGC 9 cut(s) 105, 107, 237, 342, 345, 374, 456, 570, 617
AclWI GGATC 1 cut(s) 267
AfaI GTAC 8 cut(s) 71, 128, 224, 482, 564, 583, 593, 613
AfiI CCNNNNNNNGG 3 cut(s) 144, 544, 547
AflII CTTAAG 1 cut(s) 389
AgsI TTSAA 1 cut(s) 86
AjnI CCWGG 2 cut(s) 6, 163
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 5 cut(s) 289, 335, 388, 423, 461
AluI AGCT 5 cut(s) 289, 335, 388, 423, 461
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 1 cut(s) 267
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 2 cut(s) 3, 152
ApeKI GCWGC 6 cut(s) 31, 92, 95, 365, 458, 572
AspS9I GGNCC 3 cut(s) 4, 152, 206
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 5 cut(s) 202, 233, 241, 538, 740
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 1 cut(s) 206
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 2 cut(s) 18, 200
BbvI GCAGC 6 cut(s) 43, 79, 82, 377, 470, 584
BceAI ACGGC 1 cut(s) 282
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciT130I CCWGG 2 cut(s) 8, 165
BclI TGATCA 1 cut(s) 271
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 640
BfrI CTTAAG 1 cut(s) 389
BglI GCCNNNNNGGC 1 cut(s) 692
BisI GCNGC 8 cut(s) 32, 93, 96, 105, 345, 366, 459, 573
BlsI GCNGC 8 cut(s) 33, 94, 97, 106, 346, 367, 460, 574
BmcAI AGTACT 1 cut(s) 613
Bme1390I CCNGG 3 cut(s) 8, 165, 400
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 3 cut(s) 4, 152, 206
BmiI GGNNCC 3 cut(s) 357, 657, 696
BmrFI CCNGG 3 cut(s) 8, 165, 400
BpiI GAAGAC 2 cut(s) 18, 200
BpmI CTGGAG 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 37, 50
BpuMI CCSGG 1 cut(s) 400
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 7 cut(s) 6, 45, 105, 138, 177, 399, 675
BsaXI ACNNNNNCTCC 2 cut(s) 620, 650
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 544, 547
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 315
BseBI CCWGG 2 cut(s) 8, 165
BseDI CCNNGG 7 cut(s) 6, 45, 105, 138, 177, 399, 675
BseGI GGATG 1 cut(s) 326
BseLI CCNNNNNNNGG 3 cut(s) 144, 544, 547
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 3 cut(s) 62, 65, 236
BseXI GCAGC 6 cut(s) 43, 79, 82, 377, 470, 584
BsgI GTGCAG 2 cut(s) 50, 626
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 5, 154
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 2 cut(s) 26, 399
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 3 cut(s) 144, 544, 547
BsmAI GTCTC 1 cut(s) 640
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 2 cut(s) 5, 154
Bso31I GGTCTC 1 cut(s) 640
BsoBI CYCGRG 1 cut(s) 418
Bsp1407I TGTACA 1 cut(s) 581
Bsp143I GATC 2 cut(s) 259, 271
BspACI CCGC 9 cut(s) 105, 107, 237, 342, 345, 374, 456, 570, 617
BspANI GGCC 2 cut(s) 5, 154
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 3 cut(s) 357, 657, 696
BspPI GGATC 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 640
BsrBI CCGCTC 3 cut(s) 239, 347, 374
BsrFI RCCGGY 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 581
BsrI ACTGG 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 7 cut(s) 6, 45, 105, 138, 177, 399, 675
BssMI GATC 2 cut(s) 259, 271
BssSI CACGAG 1 cut(s) 369
BssT1I CCWWGG 2 cut(s) 138, 675
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 8, 165
Bst4CI ACNGT 5 cut(s) 131, 190, 199, 604, 728
BstAFI CTTAAG 1 cut(s) 389
BstAUI TGTACA 1 cut(s) 581
BstC8I GCNNGC 4 cut(s) 23, 321, 396, 717
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 2 cut(s) 105, 177
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 1 cut(s) 326
BstFNI CGCG 1 cut(s) 107
BstKTI GATC 2 cut(s) 262, 274
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 2 cut(s) 259, 271
BstNI CCWGG 2 cut(s) 8, 165
BstSCI CCNGG 3 cut(s) 6, 163, 398
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 6 cut(s) 43, 79, 82, 377, 470, 584
BstV2I GAAGAC 2 cut(s) 18, 200
BsuRI GGCC 2 cut(s) 5, 154
BtgI CCRYGG 2 cut(s) 105, 177
BtsCI GGATG 1 cut(s) 326
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 308, 411
Cac8I GCNNGC 4 cut(s) 23, 321, 396, 717
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 3 cut(s) 4, 152, 206
Cfr42I CCGCGG 1 cut(s) 108
Csp6I GTAC 8 cut(s) 70, 127, 223, 481, 563, 582, 592, 612
CviAII CATG 2 cut(s) 101, 227
CviQI GTAC 8 cut(s) 70, 127, 223, 481, 563, 582, 592, 612
DdeI CTNAG 1 cut(s) 384
DpnI GATC 2 cut(s) 261, 273
DpnII GATC 2 cut(s) 259, 271
DraI TTTAAA 1 cut(s) 159
EciI GGCGGA 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 138, 675
Eco31I GGTCTC 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 418
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoO109I RGGNCCY 1 cut(s) 152
EcoRII CCWGG 2 cut(s) 6, 163
EcoT14I CCWWGG 2 cut(s) 138, 675
ErhI CCWWGG 2 cut(s) 138, 675
FaeI CATG 2 cut(s) 104, 230
FaiI YATR 5 cut(s) 102, 119, 228, 474, 504
FaqI GGGAC 1 cut(s) 218
FatI CATG 2 cut(s) 100, 226
FauI CCCGC 2 cut(s) 244, 463
FbaI TGATCA 1 cut(s) 271
FblI GTMKAC 3 cut(s) 213, 408, 438
Fnu4HI GCNGC 8 cut(s) 32, 93, 96, 105, 345, 366, 459, 573
FokI GGATG 1 cut(s) 313
Fsp4HI GCNGC 8 cut(s) 32, 93, 96, 105, 345, 366, 459, 573
GluI GCNGC 8 cut(s) 32, 93, 96, 105, 345, 366, 459, 573
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 2 cut(s) 5, 154
HapII CCGG 2 cut(s) 26, 399
Hin1II CATG 2 cut(s) 104, 230
HinfI GANTC 1 cut(s) 173
HpaII CCGG 2 cut(s) 26, 399
HphI GGTGA 5 cut(s) 202, 233, 241, 538, 740
Hpy166II GTNNAC 5 cut(s) 214, 409, 439, 452, 529
Hpy188I TCNGA 1 cut(s) 385
Hpy188III TCNNGA 3 cut(s) 359, 541, 698
Hpy8I GTNNAC 5 cut(s) 214, 409, 439, 452, 529
HpyAV CCTTC 4 cut(s) 176, 538, 616, 685
HpyCH4III ACNGT 5 cut(s) 131, 190, 199, 604, 728
HpyCH4V TGCA 3 cut(s) 31, 607, 719
HpyF3I CTNAG 1 cut(s) 384
Hsp92II CATG 2 cut(s) 104, 230
Ksp22I TGATCA 1 cut(s) 271
KspI CCGCGG 1 cut(s) 108
Kzo9I GATC 2 cut(s) 259, 271
LmnI GCTCC 2 cut(s) 361, 700
Lsp1109I GCAGC 6 cut(s) 43, 79, 82, 377, 470, 584
MaeIII GTNAC 3 cut(s) 280, 626, 722
MalI GATC 2 cut(s) 261, 273
MbiI CCGCTC 3 cut(s) 239, 347, 374
MboI GATC 2 cut(s) 259, 271
MboII GAAGA 2 cut(s) 23, 205
MluCI AATT 2 cut(s) 77, 669
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 184, 683
MseI TTAA 3 cut(s) 158, 315, 390
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 107
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 2 cut(s) 26, 399
MspR9I CCNGG 3 cut(s) 8, 165, 400
MvaI CCWGG 2 cut(s) 8, 165
MvnI CGCG 1 cut(s) 107
NciI CCSGG 1 cut(s) 400
NdeII GATC 2 cut(s) 259, 271
NlaIII CATG 2 cut(s) 104, 230
NlaIV GGNNCC 3 cut(s) 357, 657, 696
NmeAIII GCCGAG 2 cut(s) 520, 712
NmuCI GTSAC 1 cut(s) 722
OliI CACNNNNGTG 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 418
PkrI GCNGC 8 cut(s) 33, 94, 97, 106, 346, 367, 460, 574
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 6, 163
PspGI CCWGG 2 cut(s) 6, 163
PspN4I GGNNCC 3 cut(s) 357, 657, 696
PspPI GGNCC 3 cut(s) 4, 152, 206
PspXI VCTCGAGB 1 cut(s) 418
RsaI GTAC 8 cut(s) 71, 128, 224, 482, 564, 583, 593, 613
RsaNI GTAC 8 cut(s) 70, 127, 223, 481, 563, 582, 592, 612
RseI CAYNNNNRTG 1 cut(s) 254
SacII CCGCGG 1 cut(s) 108
SaqAI TTAA 3 cut(s) 158, 315, 390
SatI GCNGC 8 cut(s) 32, 93, 96, 105, 345, 366, 459, 573
Sau3AI GATC 2 cut(s) 259, 271
Sau96I GGNCC 3 cut(s) 4, 152, 206
ScaI AGTACT 1 cut(s) 613
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 8, 165, 400
Sfr274I CTCGAG 1 cut(s) 418
Sfr303I CCGCGG 1 cut(s) 108
SgrBI CCGCGG 1 cut(s) 108
SinI GGWCC 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 4 cut(s) 16, 65, 389, 418
SmoI CTYRAG 4 cut(s) 16, 65, 389, 418
Sse9I AATT 2 cut(s) 77, 669
SsiI CCGC 9 cut(s) 105, 107, 237, 342, 345, 374, 456, 570, 617
StyD4I CCNGG 3 cut(s) 6, 163, 398
StyI CCWWGG 2 cut(s) 138, 675
TaaI ACNGT 5 cut(s) 131, 190, 199, 604, 728
TaqI TCGA 1 cut(s) 419
TaqII GACCGA 1 cut(s) 59
TasI AATT 2 cut(s) 77, 669
TatI WGTACW 3 cut(s) 581, 591, 611
TauI GCSGC 2 cut(s) 107, 347
Tru1I TTAA 3 cut(s) 158, 315, 390
Tru9I TTAA 3 cut(s) 158, 315, 390
TscAI CASTG 2 cut(s) 315, 418
TseFI GTSAC 1 cut(s) 722
TseI GCWGC 6 cut(s) 31, 92, 95, 365, 458, 572
Tsp45I GTSAC 1 cut(s) 722
TspDTI ATGAA 1 cut(s) 106
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 2 cut(s) 315, 418
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 206
XagI CCTNNNNNAGG 1 cut(s) 542
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 3 cut(s) 213, 408, 438
ZrmI AGTACT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.