MD12G1213500.v1.1

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002647
Physical Location & Seq
Reverse (-)
29185759 .. 29188194
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1213500.v1.1.491

Sequence Viewer

Length: 753 bp
ATGGCCCAGGAAGATTTGAGCGTGCAGGTGCAGCAACAGATGACTGCGGAGGAGGAACAGAGGCTCAAGTACCTCCAATTTGTTCAAGTGGCTGCAGCTTATGCCGAGGTTTGCTTCACAAACCTTTACGGTTATGGCAAGGAAAGGTCAGGGCCCTTGAAGCCTGGCGTTGAGTCCGTGGAGGGAACAGTGAAGACCGTTGTTGGACCTGTCTACAACAAGTACCATGATATCCCTGCTCACCTCCTCACCTTTGTGGATCGCAAGGTTGATTTGTCTGTTACCAAGCTGGACAGCCGTGTGCCACCAGTGGTTAAGCAGGCCTCATCCAAAGCTCTGTCCGCCGCTCAAAAGGCCCCAGAGGCAGCACGAGTGGTTGCCTCTGAGCTTAAGCGTGCCGGGGTTGTAGACACAGCCTCGAGCTGTGCTAAGTCTGTCTACTCTATGTGCGAACCCACGGCCAAAGACTTGTATGCTAAGTACGAACCAAAAGCCGAGCAGTGTGCTGTTTCTGCATGGCGAAAGGTGAATCAAGTCCTCCCAAAGGCGGTTGATGTTGTTGTACCAACCGCAGCATATTGTTCCGAAAAGTACAACCAGACCGTGCAGAGCACTGCGGAGAAGGGCTACCGGGTCTCCTCCTACCTGCCCTTGGTTCCCACAGAGAAAATCGCCATGGTTTTCGCAGAGAAAGCGCCCGAAGCAGAGCCTTTGGTTGCAAGCCACGGTGAAGCTGATGTTGCCATTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

251

Amino Acids

27.19

Weight (kDa)

6.9

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 17 - 229 6.9e-86 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
Acc36I ACCTGC 2 cut(s) 16, 654
AccBSI CCGCTC 1 cut(s) 347
AccI GTMKAC 3 cut(s) 213, 408, 438
AciI CCGC 6 cut(s) 47, 342, 345, 548, 570, 617
AclWI GGATC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 459
AfaI GTAC 5 cut(s) 71, 224, 482, 564, 593
AfiI CCNNNNNNNGG 3 cut(s) 544, 547, 652
AflII CTTAAG 1 cut(s) 389
AgsI TTSAA 2 cut(s) 86, 160
AjnI CCWGG 2 cut(s) 6, 163
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 6 cut(s) 98, 289, 335, 388, 423, 734
AluI AGCT 6 cut(s) 98, 289, 335, 388, 423, 734
Alw21I GWGCWC 1 cut(s) 614
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 1 cut(s) 267
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 5 cut(s) 3, 152, 321, 354, 459
ApaI GGGCCC 1 cut(s) 156
ApeKI GCWGC 5 cut(s) 31, 92, 95, 365, 572
AspLEI GCGC 1 cut(s) 697
AspS9I GGNCC 5 cut(s) 4, 152, 153, 206, 355
AsuC2I CCSGG 2 cut(s) 400, 632
AsuHPI GGTGA 4 cut(s) 233, 241, 538, 740
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 156
BanII GRGCYC 1 cut(s) 156
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 5 cut(s) 43, 79, 107, 377, 584
BceAI ACGGC 2 cut(s) 282, 474
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciT130I CCWGG 2 cut(s) 8, 165
BcnI CCSGG 2 cut(s) 400, 632
BcoDI GTCTC 1 cut(s) 640
BfmI CTRYAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 698
BfrI CTTAAG 1 cut(s) 389
BfuAI ACCTGC 2 cut(s) 16, 654
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 6 cut(s) 32, 93, 96, 345, 366, 573
BlsI GCNGC 6 cut(s) 33, 94, 97, 346, 367, 574
Bme1390I CCNGG 4 cut(s) 8, 165, 400, 632
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 5 cut(s) 4, 152, 153, 206, 355
BmiI GGNNCC 3 cut(s) 154, 357, 657
BmrFI CCNGG 4 cut(s) 8, 165, 400, 632
BpiI GAAGAC 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 2 cut(s) 400, 632
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 8 cut(s) 6, 105, 177, 399, 456, 651, 675, 724
BsaXI ACNNNNNCTCC 2 cut(s) 620, 650
Bsc4I CCNNNNNNNGG 3 cut(s) 544, 547, 652
Bse1I ACTGG 1 cut(s) 308
BseBI CCWGG 2 cut(s) 8, 165
BseDI CCNNGG 8 cut(s) 6, 105, 177, 399, 456, 651, 675, 724
BseGI GGATG 1 cut(s) 326
BseLI CCNNNNNNNGG 3 cut(s) 544, 547, 652
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 308
BseRI GAGGAG 3 cut(s) 65, 236, 628
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 5 cut(s) 43, 79, 107, 377, 584
BsgI GTGCAG 3 cut(s) 44, 50, 626
BshFI GGCC 5 cut(s) 5, 154, 323, 356, 461
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 2 cut(s) 399, 631
BslI CCNNNNNNNGG 3 cut(s) 544, 547, 652
BsmAI GTCTC 1 cut(s) 640
BsnI GGCC 5 cut(s) 5, 154, 323, 356, 461
Bso31I GGTCTC 1 cut(s) 640
BsoBI CYCGRG 1 cut(s) 418
Bsp120I GGGCCC 1 cut(s) 152
Bsp1286I GDGCHC 2 cut(s) 156, 614
Bsp143I GATC 1 cut(s) 259
Bsp19I CCATGG 1 cut(s) 675
BspACI CCGC 6 cut(s) 47, 342, 345, 548, 570, 617
BspANI GGCC 5 cut(s) 5, 154, 323, 356, 461
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 3 cut(s) 154, 357, 657
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 2 cut(s) 16, 654
BspPI GGATC 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 347
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 8 cut(s) 6, 105, 177, 399, 456, 651, 675, 724
BssMI GATC 1 cut(s) 259
BssSI CACGAG 1 cut(s) 369
BssT1I CCWWGG 2 cut(s) 651, 675
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 8, 165
Bst4CI ACNGT 5 cut(s) 131, 190, 199, 604, 728
BstAFI CTTAAG 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 101
BstC8I GCNNGC 4 cut(s) 23, 321, 396, 721
BstDEI CTNAG 3 cut(s) 384, 429, 477
BstDSI CCRYGG 4 cut(s) 177, 456, 675, 724
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 1 cut(s) 326
BstH2I RGCGCY 1 cut(s) 698
BstHHI GCGC 1 cut(s) 697
BstKTI GATC 1 cut(s) 262
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 1 cut(s) 259
BstNI CCWGG 2 cut(s) 8, 165
BstSCI CCNGG 4 cut(s) 6, 163, 398, 630
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 156
BstV1I GCAGC 5 cut(s) 43, 79, 107, 377, 584
BstV2I GAAGAC 1 cut(s) 200
BsuRI GGCC 5 cut(s) 5, 154, 323, 356, 461
BtgI CCRYGG 4 cut(s) 177, 456, 675, 724
BtsCI GGATG 1 cut(s) 326
BtsI GCAGTG 2 cut(s) 506, 612
BtsIMutI CAGTG 4 cut(s) 195, 315, 506, 612
BveI ACCTGC 2 cut(s) 16, 654
Cac8I GCNNGC 4 cut(s) 23, 321, 396, 721
CfoI GCGC 1 cut(s) 697
Cfr13I GGNCC 5 cut(s) 4, 152, 153, 206, 355
Csp6I GTAC 5 cut(s) 70, 223, 481, 563, 592
CviAII CATG 3 cut(s) 227, 516, 676
CviQI GTAC 5 cut(s) 70, 223, 481, 563, 592
DdeI CTNAG 3 cut(s) 384, 429, 477
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 459
EciI GGCGGA 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 651, 675
Eco147I AGGCCT 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 640
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 418
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoO109I RGGNCCY 3 cut(s) 152, 153, 355
EcoRII CCWGG 2 cut(s) 6, 163
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 651, 675
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 2 cut(s) 651, 675
FaeI CATG 3 cut(s) 230, 519, 679
FaiI YATR 8 cut(s) 102, 135, 228, 446, 474, 517, 577, 677
FatI CATG 3 cut(s) 226, 515, 675
FblI GTMKAC 3 cut(s) 213, 408, 438
Fnu4HI GCNGC 6 cut(s) 32, 93, 96, 345, 366, 573
FokI GGATG 1 cut(s) 313
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 6 cut(s) 32, 93, 96, 345, 366, 573
GlaI GCGC 1 cut(s) 696
GluI GCNGC 6 cut(s) 32, 93, 96, 345, 366, 573
HaeII RGCGCY 1 cut(s) 698
HaeIII GGCC 5 cut(s) 5, 154, 323, 356, 461
HapII CCGG 2 cut(s) 399, 631
HhaI GCGC 1 cut(s) 697
Hin1II CATG 3 cut(s) 230, 519, 679
Hin6I GCGC 1 cut(s) 695
HinP1I GCGC 1 cut(s) 695
HinfI GANTC 2 cut(s) 173, 529
HpaII CCGG 2 cut(s) 399, 631
HphI GGTGA 4 cut(s) 233, 241, 538, 740
Hpy166II GTNNAC 3 cut(s) 214, 409, 439
Hpy188I TCNGA 2 cut(s) 385, 586
Hpy8I GTNNAC 3 cut(s) 214, 409, 439
HpyAV CCTTC 1 cut(s) 616
HpyCH4III ACNGT 5 cut(s) 131, 190, 199, 604, 728
HpyCH4V TGCA 6 cut(s) 25, 31, 95, 515, 607, 719
HpyF3I CTNAG 3 cut(s) 384, 429, 477
Hsp92II CATG 3 cut(s) 230, 519, 679
HspAI GCGC 1 cut(s) 695
Kzo9I GATC 1 cut(s) 259
Lsp1109I GCAGC 5 cut(s) 43, 79, 107, 377, 584
MaeIII GTNAC 1 cut(s) 280
MalI GATC 1 cut(s) 261
MbiI CCGCTC 1 cut(s) 347
MboI GATC 1 cut(s) 259
MboII GAAGA 2 cut(s) 23, 205
MhlI GDGCHC 2 cut(s) 156, 614
MluCI AATT 1 cut(s) 77
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 184
MseI TTAA 2 cut(s) 315, 390
MslI CAYNNNNRTG 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 2 cut(s) 399, 631
MspR9I CCNGG 4 cut(s) 8, 165, 400, 632
MvaI CCWGG 2 cut(s) 8, 165
NciI CCSGG 2 cut(s) 400, 632
NcoI CCATGG 1 cut(s) 675
NdeII GATC 1 cut(s) 259
NlaIII CATG 3 cut(s) 230, 519, 679
NlaIV GGNNCC 3 cut(s) 154, 357, 657
NmeAIII GCCGAG 2 cut(s) 130, 520
OliI CACNNNNGTG 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 418
PaqCI CACCTGC 1 cut(s) 16
PceI AGGCCT 1 cut(s) 323
PfeI GAWTC 1 cut(s) 529
PkrI GCNGC 6 cut(s) 33, 94, 97, 346, 367, 574
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 6, 163
PspGI CCWGG 2 cut(s) 6, 163
PspN4I GGNNCC 3 cut(s) 154, 357, 657
PspOMI GGGCCC 1 cut(s) 152
PspPI GGNCC 5 cut(s) 4, 152, 153, 206, 355
PspXI VCTCGAGB 1 cut(s) 418
PstI CTGCAG 1 cut(s) 97
RsaI GTAC 5 cut(s) 71, 224, 482, 564, 593
RsaNI GTAC 5 cut(s) 70, 223, 481, 563, 592
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 2 cut(s) 315, 390
SatI GCNGC 6 cut(s) 32, 93, 96, 345, 366, 573
Sau3AI GATC 1 cut(s) 259
Sau96I GGNCC 5 cut(s) 4, 152, 153, 206, 355
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 4 cut(s) 8, 165, 400, 632
SduI GDGCHC 2 cut(s) 156, 614
SfcI CTRYAG 1 cut(s) 93
Sfr274I CTCGAG 1 cut(s) 418
SinI GGWCC 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 3 cut(s) 65, 389, 418
SmoI CTYRAG 3 cut(s) 65, 389, 418
Sse9I AATT 1 cut(s) 77
SseBI AGGCCT 1 cut(s) 323
SsiI CCGC 6 cut(s) 47, 342, 345, 548, 570, 617
StuI AGGCCT 1 cut(s) 323
StyD4I CCNGG 4 cut(s) 6, 163, 398, 630
StyI CCWWGG 2 cut(s) 651, 675
TaaI ACNGT 5 cut(s) 131, 190, 199, 604, 728
TaqI TCGA 1 cut(s) 419
TasI AATT 1 cut(s) 77
TatI WGTACW 1 cut(s) 591
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 1 cut(s) 529
Tru1I TTAA 2 cut(s) 315, 390
Tru9I TTAA 2 cut(s) 315, 390
TscAI CASTG 4 cut(s) 195, 315, 506, 619
TseI GCWGC 5 cut(s) 31, 92, 95, 365, 572
TspDTI ATGAA 1 cut(s) 737
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 4 cut(s) 195, 315, 506, 619
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 206
XagI CCTNNNNNAGG 1 cut(s) 542
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 3 cut(s) 213, 408, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.