FvH4_6g06530

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002647
Physical Location & Seq
Forward (+)
3828324 .. 3830056
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06530.t1

Sequence Viewer

Length: 675 bp
ATGGCCCAACAAGATTCAACCGTGCAGGAACCACAAATGAAGGAGGAGCAGAGTCTCAAGTATTTGGGATTTGTGCAAAACGCCGCCGTTGCGTCCGGGGTGTGCGTTTCGAAAGCGTACGGGTATGCCAAGGAGAGGTCTGGTCCTTTGAAGAATGGGGTTGATTCTGTGGAGGGGAAGGTCAAGACCGTTGTTGGACCGGTTTATGTCAAGTACCAGGAAGCCTCGGCCCAAATTCTCACCTTTGTCGATTGCAAGGTTGATGAATCTGTGACCAGGGCTGCCAAGCTAGACGTGCGTCCAGTGATCAGGCAGGCCTCCTCCAAAGCCTTATCAGTTGCTCAGAAGGCACCAGAGGCGGCTTGCACAGCTGCTAAATCTGCCTACACCAAGTATGAACCAAAAGCTGAGGAATGTGCTGCTTCTGCGTGGCGAAAGCTGAATGAACTCCCCGTCTTCCCTAAGGTGGCGGAGGTGGTTGTGCCAGCTGCAGCATATTGTTCTGAAAAGTACAATGAGACTGTGCAACACACTGCCGAGAAGGGGTACAGAGTTGCTGCCTATATGCCTTTGGTTCCCACAGAGAGAATCGCCAAGGTCTTCGGAGAGAAGGTGCCTGAACCAGAGCCAGTGATTGCAGCAAGTCATGGGGAGACTGATATTGCTGTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

225

Amino Acids

24.17

Weight (kDa)

7.56

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 14 - 202 1.5e-65 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 349, 613
AciI CCGC 3 cut(s) 84, 359, 470
AcsI RAATTY 1 cut(s) 234
AfaI GTAC 4 cut(s) 119, 215, 512, 548
AfiI CCNNNNNNNGG 3 cut(s) 135, 466, 543
AgeI ACCGGT 1 cut(s) 199
AgsI TTSAA 3 cut(s) 18, 151, 671
AjiI CACGTC 1 cut(s) 295
AjnI CCWGG 2 cut(s) 216, 275
AluBI AGCT 5 cut(s) 289, 371, 407, 439, 488
AluI AGCT 5 cut(s) 289, 371, 407, 439, 488
Alw26I GTCTC 3 cut(s) 59, 512, 647
AoxI GGCC 3 cut(s) 3, 228, 315
ApeKI GCWGC 7 cut(s) 281, 371, 419, 488, 491, 557, 638
ApoI RAATTY 1 cut(s) 234
AsiGI ACCGGT 1 cut(s) 199
AspS9I GGNCC 4 cut(s) 4, 143, 197, 229
AsuC2I CCSGG 1 cut(s) 97
AsuHPI GGTGA 1 cut(s) 232
AsuII TTCGAA 1 cut(s) 110
AvaII GGWCC 2 cut(s) 143, 197
AxyI CCTNAGG 1 cut(s) 462
BanI GGYRCC 2 cut(s) 349, 613
BbsI GAAGAC 2 cut(s) 448, 592
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 7 cut(s) 268, 358, 406, 475, 503, 544, 650
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 2 cut(s) 218, 277
BclI TGATCA 1 cut(s) 306
BcnI CCSGG 1 cut(s) 97
BcoDI GTCTC 3 cut(s) 59, 512, 647
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 489
BisI GCNGC 9 cut(s) 84, 282, 360, 372, 420, 489, 492, 558, 639
BlsI GCNGC 9 cut(s) 85, 283, 361, 373, 421, 490, 493, 559, 640
Bme1390I CCNGG 3 cut(s) 97, 218, 277
Bme18I GGWCC 2 cut(s) 143, 197
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 4 cut(s) 4, 143, 197, 229
BmiI GGNNCC 4 cut(s) 30, 351, 576, 615
BmrFI CCNGG 3 cut(s) 97, 218, 277
BoxI GACNNNNGTC 1 cut(s) 297
BpiI GAAGAC 2 cut(s) 448, 592
Bpu10I CCTNAGC 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 97
BsaJI CCNNGG 5 cut(s) 96, 129, 225, 276, 594
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 466, 543
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 2 cut(s) 302, 629
Bse21I CCTNAGG 1 cut(s) 462
BseBI CCWGG 2 cut(s) 218, 277
BseDI CCNNGG 5 cut(s) 96, 129, 225, 276, 594
BseLI CCNNNNNNNGG 3 cut(s) 135, 466, 543
BseMII CTCAG 2 cut(s) 356, 399
BseNI ACTGG 2 cut(s) 302, 629
BseRI GAGGAG 2 cut(s) 59, 310
BseXI GCAGC 7 cut(s) 268, 358, 406, 475, 503, 544, 650
BsgI GTGCAG 1 cut(s) 44
BshFI GGCC 3 cut(s) 5, 230, 317
BshNI GGYRCC 2 cut(s) 349, 613
BshTI ACCGGT 1 cut(s) 199
BsiSI CCGG 2 cut(s) 96, 200
BsiWI CGTACG 1 cut(s) 117
BslI CCNNNNNNNGG 3 cut(s) 135, 466, 543
BsmAI GTCTC 3 cut(s) 59, 512, 647
BsnI GGCC 3 cut(s) 5, 230, 317
Bsp119I TTCGAA 1 cut(s) 110
Bsp143I GATC 1 cut(s) 306
BspACI CCGC 3 cut(s) 84, 359, 470
BspANI GGCC 3 cut(s) 5, 230, 317
BspCNI CTCAG 2 cut(s) 355, 400
BspLI GGNNCC 4 cut(s) 30, 351, 576, 615
BspMAI CTGCAG 1 cut(s) 493
BspT104I TTCGAA 1 cut(s) 110
BspT107I GGYRCC 2 cut(s) 349, 613
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 2 cut(s) 302, 629
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 5 cut(s) 96, 129, 225, 276, 594
BssMI GATC 1 cut(s) 306
BssT1I CCWWGG 2 cut(s) 129, 594
Bst2UI CCWGG 2 cut(s) 218, 277
Bst4CI ACNGT 3 cut(s) 22, 190, 523
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 3 cut(s) 315, 364, 486
BstDEI CTNAG 3 cut(s) 342, 408, 462
BstKTI GATC 1 cut(s) 309
BstMAI GTCTC 3 cut(s) 59, 512, 647
BstMBI GATC 1 cut(s) 306
BstMWI GCNNNNNNNGC 7 cut(s) 89, 295, 347, 356, 368, 380, 425
BstNI CCWGG 2 cut(s) 218, 277
BstPAI GACNNNNGTC 1 cut(s) 297
BstSCI CCNGG 3 cut(s) 95, 216, 275
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 7 cut(s) 268, 358, 406, 475, 503, 544, 650
BstV2I GAAGAC 2 cut(s) 448, 592
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 3 cut(s) 5, 230, 317
BtrI CACGTC 1 cut(s) 295
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 3 cut(s) 309, 531, 636
Cac8I GCNNGC 3 cut(s) 315, 364, 486
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 4 cut(s) 4, 143, 197, 229
CseI GACGC 2 cut(s) 81, 287
Csp6I GTAC 4 cut(s) 118, 214, 511, 547
CspAI ACCGGT 1 cut(s) 199
CviAII CATG 1 cut(s) 647
CviQI GTAC 4 cut(s) 118, 214, 511, 547
DdeI CTNAG 3 cut(s) 342, 408, 462
DpnI GATC 1 cut(s) 308
DpnII GATC 1 cut(s) 306
EciI GGCGGA 1 cut(s) 485
Eco130I CCWWGG 2 cut(s) 129, 594
Eco147I AGGCCT 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 143, 197
Eco81I CCTNAGG 1 cut(s) 462
EcoRII CCWGG 2 cut(s) 216, 275
EcoT14I CCWWGG 2 cut(s) 129, 594
ErhI CCWWGG 2 cut(s) 129, 594
FaeI CATG 1 cut(s) 650
FaiI YATR 7 cut(s) 126, 207, 396, 496, 564, 566, 648
FatI CATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 306
Fnu4HI GCNGC 9 cut(s) 84, 282, 360, 372, 420, 489, 492, 558, 639
Fsp4HI GCNGC 9 cut(s) 84, 282, 360, 372, 420, 489, 492, 558, 639
FspBI CTAG 1 cut(s) 290
GluI GCNGC 9 cut(s) 84, 282, 360, 372, 420, 489, 492, 558, 639
HaeIII GGCC 3 cut(s) 5, 230, 317
HapII CCGG 2 cut(s) 96, 200
HgaI GACGC 2 cut(s) 81, 287
Hin1II CATG 1 cut(s) 650
HinfI GANTC 5 cut(s) 14, 52, 164, 266, 588
HpaII CCGG 2 cut(s) 96, 200
HphI GGTGA 1 cut(s) 232
Hpy188I TCNGA 3 cut(s) 345, 505, 605
Hpy188III TCNNGA 1 cut(s) 184
HpyAV CCTTC 5 cut(s) 34, 172, 340, 535, 604
HpyCH4III ACNGT 3 cut(s) 22, 190, 523
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 7 cut(s) 25, 76, 255, 366, 491, 526, 638
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 295, 347, 356, 368, 380, 425
HpyF3I CTNAG 3 cut(s) 342, 408, 462
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 1 cut(s) 650
Ksp22I TGATCA 1 cut(s) 306
Kzo9I GATC 1 cut(s) 306
LmnI GCTCC 1 cut(s) 46
Lsp1109I GCAGC 7 cut(s) 268, 358, 406, 475, 503, 544, 650
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 1 cut(s) 271
MalI GATC 1 cut(s) 308
MboI GATC 1 cut(s) 306
MboII GAAGA 3 cut(s) 163, 448, 592
MluCI AATT 1 cut(s) 234
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 9 cut(s) 37, 129, 166, 235, 328, 331, 349, 403, 466
MspA1I CMGCKG 2 cut(s) 371, 488
MspI CCGG 2 cut(s) 96, 200
MspR9I CCNGG 3 cut(s) 97, 218, 277
MvaI CCWGG 2 cut(s) 218, 277
MwoI GCNNNNNNNGC 7 cut(s) 89, 295, 347, 356, 368, 380, 425
NciI CCSGG 1 cut(s) 97
NdeII GATC 1 cut(s) 306
NlaIII CATG 1 cut(s) 650
NlaIV GGNNCC 4 cut(s) 30, 351, 576, 615
NmeAIII GCCGAG 2 cut(s) 206, 562
NmuCI GTSAC 1 cut(s) 271
NspV TTCGAA 1 cut(s) 110
PceI AGGCCT 1 cut(s) 317
PfeI GAWTC 4 cut(s) 14, 164, 266, 588
Pfl23II CGTACG 1 cut(s) 117
PinAI ACCGGT 1 cut(s) 199
PkrI GCNGC 9 cut(s) 85, 283, 361, 373, 421, 490, 493, 559, 640
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
PshAI GACNNNNGTC 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 216, 275
PspGI CCWGG 2 cut(s) 216, 275
PspLI CGTACG 1 cut(s) 117
PspN4I GGNNCC 4 cut(s) 30, 351, 576, 615
PspPI GGNCC 4 cut(s) 4, 143, 197, 229
PstI CTGCAG 1 cut(s) 493
PvuII CAGCTG 2 cut(s) 371, 488
RsaI GTAC 4 cut(s) 119, 215, 512, 548
RsaNI GTAC 4 cut(s) 118, 214, 511, 547
SatI GCNGC 9 cut(s) 84, 282, 360, 372, 420, 489, 492, 558, 639
Sau3AI GATC 1 cut(s) 306
Sau96I GGNCC 4 cut(s) 4, 143, 197, 229
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 3 cut(s) 97, 218, 277
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 110
SinI GGWCC 2 cut(s) 143, 197
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
Sse9I AATT 1 cut(s) 234
SseBI AGGCCT 1 cut(s) 317
SsiI CCGC 3 cut(s) 84, 359, 470
SspMI CTAG 1 cut(s) 290
StuI AGGCCT 1 cut(s) 317
StyD4I CCNGG 3 cut(s) 95, 216, 275
StyI CCWWGG 2 cut(s) 129, 594
TaaI ACNGT 3 cut(s) 22, 190, 523
TaiI ACGT 1 cut(s) 297
TaqI TCGA 2 cut(s) 110, 249
TasI AATT 1 cut(s) 234
TatI WGTACW 1 cut(s) 510
TauI GCSGC 2 cut(s) 86, 362
TfiI GAWTC 4 cut(s) 14, 164, 266, 588
TscAI CASTG 3 cut(s) 309, 538, 636
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 7 cut(s) 281, 371, 419, 488, 491, 557, 638
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 4 cut(s) 53, 279, 411, 459
TspRI CASTG 3 cut(s) 309, 538, 636
VpaK11BI GGWCC 2 cut(s) 143, 197
XapI RAATTY 1 cut(s) 234
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.