FvH4_1g27660

expansin-A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010451
Physical Location & Seq
Reverse (-)
19303774 .. 19305253
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27660.t2

Sequence Viewer

Length: 759 bp
ATGTCGCCGCCGACCCTAACGCCGCTGCTCCTCCTCCTCCTATTCACACCACCCCTCCTCTCTCACTCAATCTACGAATGGCAGTCCGCACGCGCCACCTACTACGCCGCCTCCGACCCCCGCGACGCGGTGGGCGGCGCGTGCGGCTACGGCGACCTGATGAAGGGCGGTTTCGGAATGGCGACGGTGGGGCTGAGCGAGGCGCTGTTCGAGCGTGGCCAGATCTGCGGCGCGTGCTTGGAGGTCCGGTGCGTGGAGGACATGCGGTGGTGCATTCCCGGAACCTCCATCATATTAACGGCGACGAACTTCTGCGCTCCGAACTACGGCTTCACCGCGGACGGTGGGGGCCACTGTAACCCTCCCAATAAGCACTTGGTCCTCCCGATTGAGGCCTTTGAGAAGATCGCTATCTGGAAAGCTGGTAATATGCCGGTCCAGTATCGCAGGATCAAGTGTAGAAAGGAGGGAGGAGTTCGTTTTACTATAGATGGGTCTAGTATCTTTATTTCCGTGCTGATCAGCAATGTTGCCGGCGCTGGAGACATAGCTGCAGTGAAGATCAAGGGTTCAACAACTGGTTGGCTTCCAATGGGACGGAATTGGGGTCAAAACTGGCATATAAATGCTGATTTGAAGAATCAGCCTCTTTCTTTTGAGGTCACTAGTAGCGATGGGTTGACGCTCACATCTTACAACGTTGCTCCCAAGAATTGGAACTTTGGGCAGACTTTTGAAGGGAAGCAGTTCGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

253

Amino Acids

27.33

Weight (kDa)

7.55

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Expansin_C PF01357 87 - 164 1.4e-29 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 714
AccII CGCG 6 cut(s) 93, 123, 128, 140, 233, 338
AclI AACGTT 1 cut(s) 699
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 217
AfiI CCNNNNNNNGG 6 cut(s) 127, 163, 253, 326, 391, 714
AgsI TTSAA 3 cut(s) 573, 637, 737
AhlI ACTAGT 1 cut(s) 665
AluBI AGCT 2 cut(s) 422, 551
AluI AGCT 2 cut(s) 422, 551
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 1 cut(s) 458
AoxI GGCC 3 cut(s) 217, 349, 393
ApeKI GCWGC 2 cut(s) 25, 551
Asp700I GAANNNNTTC 1 cut(s) 746
AspLEI GCGC 6 cut(s) 95, 140, 205, 233, 317, 539
AspS9I GGNCC 4 cut(s) 244, 349, 379, 436
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 1 cut(s) 325
AvaII GGWCC 3 cut(s) 244, 379, 436
BalI TGGCCA 1 cut(s) 219
BbvI GCAGC 2 cut(s) 12, 538
BccI CCATC 3 cut(s) 296, 485, 668
BceAI ACGGC 3 cut(s) 166, 315, 343
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 537
BcuI ACTAGT 1 cut(s) 665
BfaI CTAG 2 cut(s) 498, 666
BfmI CTRYAG 2 cut(s) 486, 552
BfoI RGCGCY 2 cut(s) 206, 540
BglII AGATCT 1 cut(s) 222
BisI GCNGC 8 cut(s) 8, 23, 26, 108, 136, 145, 229, 552
BlpI GCTNAGC 1 cut(s) 194
BlsI GCNGC 8 cut(s) 9, 24, 27, 109, 137, 146, 230, 553
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 3 cut(s) 244, 379, 436
BmgT120I GGNCC 4 cut(s) 244, 349, 379, 436
BmiI GGNNCC 2 cut(s) 283, 350
BmrFI CCNGG 1 cut(s) 279
BpmI CTGGAG 1 cut(s) 561
Bpu1102I GCTNAGC 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 410
BsaJI CCNNGG 1 cut(s) 336
BsaWI WCCGGW 1 cut(s) 246
BsaXI ACNNNNNCTCC 6 cut(s) 39, 69, 95, 125, 233, 263
Bsc4I CCNNNNNNNGG 6 cut(s) 127, 163, 253, 326, 391, 714
Bse118I RCCGGY 2 cut(s) 433, 533
Bse1I ACTGG 3 cut(s) 439, 583, 620
Bse3DI GCAATG 1 cut(s) 532
Bse8I GATNNNNATC 1 cut(s) 410
BseDI CCNNGG 1 cut(s) 336
BseJI GATNNNNATC 1 cut(s) 410
BseLI CCNNNNNNNGG 6 cut(s) 127, 163, 253, 326, 391, 714
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 3 cut(s) 439, 583, 620
BseRI GAGGAG 5 cut(s) 20, 23, 26, 47, 486
BseXI GCAGC 2 cut(s) 12, 538
Bsh1236I CGCG 6 cut(s) 93, 123, 128, 140, 233, 338
BshFI GGCC 3 cut(s) 219, 351, 395
BsiSI CCGG 4 cut(s) 247, 279, 434, 534
BslFI GGGAC 1 cut(s) 609
BslI CCNNNNNNNGG 6 cut(s) 127, 163, 253, 326, 391, 714
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 609
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 3 cut(s) 219, 351, 395
Bsp143I GATC 5 cut(s) 222, 405, 450, 519, 561
Bsp1720I GCTNAGC 1 cut(s) 194
BspANI GGCC 3 cut(s) 219, 351, 395
BspCNI CTCAG 1 cut(s) 186
BspFNI CGCG 6 cut(s) 93, 123, 128, 140, 233, 338
BspLI GGNNCC 2 cut(s) 283, 350
BspMAI CTGCAG 1 cut(s) 556
BspPI GGATC 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 2 cut(s) 433, 533
BsrI ACTGG 3 cut(s) 439, 583, 620
BssAI RCCGGY 2 cut(s) 433, 533
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 5 cut(s) 222, 405, 450, 519, 561
Bst4CI ACNGT 3 cut(s) 187, 344, 356
BstC8I GCNNGC 4 cut(s) 91, 142, 235, 535
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 1 cut(s) 336
BstENI CCTNNNNNAGG 1 cut(s) 161
BstFNI CGCG 6 cut(s) 93, 123, 128, 140, 233, 338
BstH2I RGCGCY 2 cut(s) 206, 540
BstHHI GCGC 6 cut(s) 95, 140, 205, 233, 317, 539
BstKTI GATC 5 cut(s) 225, 408, 453, 522, 564
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 5 cut(s) 222, 405, 450, 519, 561
BstMWI GCNNNNNNNGC 6 cut(s) 141, 144, 150, 211, 225, 234
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 277
BstSFI CTRYAG 2 cut(s) 486, 552
BstUI CGCG 6 cut(s) 93, 123, 128, 140, 233, 338
BstV1I GCAGC 2 cut(s) 12, 538
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuRI GGCC 3 cut(s) 219, 351, 395
BtgI CCRYGG 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 687
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 2 cut(s) 352, 561
Cac8I GCNNGC 4 cut(s) 91, 142, 235, 535
CfoI GCGC 6 cut(s) 95, 140, 205, 233, 317, 539
Cfr10I RCCGGY 2 cut(s) 433, 533
Cfr13I GGNCC 4 cut(s) 244, 349, 379, 436
Cfr42I CCGCGG 1 cut(s) 339
CseI GACGC 2 cut(s) 134, 691
CviAII CATG 1 cut(s) 262
DdeI CTNAG 1 cut(s) 194
DpnI GATC 5 cut(s) 224, 407, 452, 521, 563
DpnII GATC 5 cut(s) 222, 405, 450, 519, 561
EaeI YGGCCR 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 395
Eco47I GGWCC 3 cut(s) 244, 379, 436
EcoNI CCTNNNNNAGG 1 cut(s) 161
FaeI CATG 1 cut(s) 265
FaiI YATR 7 cut(s) 263, 293, 431, 488, 548, 621, 623
FaqI GGGAC 1 cut(s) 609
FatI CATG 1 cut(s) 261
FauI CCCGC 1 cut(s) 128
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 8 cut(s) 8, 23, 26, 108, 136, 145, 229, 552
Fsp4HI GCNGC 8 cut(s) 8, 23, 26, 108, 136, 145, 229, 552
FspBI CTAG 2 cut(s) 498, 666
GlaI GCGC 6 cut(s) 94, 139, 204, 232, 316, 538
GluI GCNGC 8 cut(s) 8, 23, 26, 108, 136, 145, 229, 552
GsuI CTGGAG 1 cut(s) 561
HaeII RGCGCY 2 cut(s) 206, 540
HaeIII GGCC 3 cut(s) 219, 351, 395
HapII CCGG 4 cut(s) 247, 279, 434, 534
HgaI GACGC 2 cut(s) 134, 691
HhaI GCGC 6 cut(s) 95, 140, 205, 233, 317, 539
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 6 cut(s) 93, 138, 203, 231, 315, 537
HinP1I GCGC 6 cut(s) 93, 138, 203, 231, 315, 537
HincII GTYRAC 1 cut(s) 681
HindII GTYRAC 1 cut(s) 681
HinfI GANTC 2 cut(s) 640, 752
HpaII CCGG 4 cut(s) 247, 279, 434, 534
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 1 cut(s) 681
Hpy188I TCNGA 3 cut(s) 115, 176, 321
Hpy188III TCNNGA 3 cut(s) 385, 415, 756
Hpy8I GTNNAC 1 cut(s) 681
Hpy99I CGWCG 3 cut(s) 128, 187, 307
HpyAV CCTTC 2 cut(s) 157, 731
HpyCH4III ACNGT 3 cut(s) 187, 344, 356
HpyCH4IV ACGT 1 cut(s) 699
HpyCH4V TGCA 2 cut(s) 273, 554
HpyF10VI GCNNNNNNNGC 6 cut(s) 141, 144, 150, 211, 225, 234
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 1 cut(s) 699
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 6 cut(s) 93, 138, 203, 231, 315, 537
KroI GCCGGC 1 cut(s) 533
KroNI GCCGGC 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 519
KspI CCGCGG 1 cut(s) 339
Kzo9I GATC 5 cut(s) 222, 405, 450, 519, 561
LmnI GCTCC 3 cut(s) 33, 322, 709
Lsp1109I GCAGC 2 cut(s) 12, 538
MaeI CTAG 2 cut(s) 498, 666
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 2 cut(s) 356, 661
MalI GATC 5 cut(s) 224, 407, 452, 521, 563
MboI GATC 5 cut(s) 222, 405, 450, 519, 561
MboII GAAGA 3 cut(s) 415, 571, 649
MflI RGATCY 1 cut(s) 222
MlsI TGGCCA 1 cut(s) 219
MluCI AATT 2 cut(s) 601, 712
MluNI TGGCCA 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 138
Mox20I TGGCCA 1 cut(s) 219
MroNI GCCGGC 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 746
MscI TGGCCA 1 cut(s) 219
MseI TTAA 1 cut(s) 296
MslI CAYNNNNRTG 1 cut(s) 624
Msp20I TGGCCA 1 cut(s) 219
MspA1I CMGCKG 2 cut(s) 25, 338
MspI CCGG 4 cut(s) 247, 279, 434, 534
MspR9I CCNGG 1 cut(s) 279
Mva1269I GAATGC 1 cut(s) 273
MvnI CGCG 6 cut(s) 93, 123, 128, 140, 233, 338
MwoI GCNNNNNNNGC 6 cut(s) 141, 144, 150, 211, 225, 234
NaeI GCCGGC 1 cut(s) 535
NciI CCSGG 1 cut(s) 279
NdeII GATC 5 cut(s) 222, 405, 450, 519, 561
NgoMIV GCCGGC 1 cut(s) 533
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 2 cut(s) 283, 350
NmuCI GTSAC 1 cut(s) 661
NspI RCATGY 1 cut(s) 265
PceI AGGCCT 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 746
PfeI GAWTC 1 cut(s) 640
PflMI CCANNNNNTGG 1 cut(s) 714
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 8 cut(s) 9, 24, 27, 109, 137, 146, 230, 553
Psp1406I AACGTT 1 cut(s) 699
PspN4I GGNNCC 2 cut(s) 283, 350
PspPI GGNCC 4 cut(s) 244, 349, 379, 436
PstI CTGCAG 1 cut(s) 556
PsuI RGATCY 1 cut(s) 222
RseI CAYNNNNRTG 1 cut(s) 624
SacII CCGCGG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 296
SatI GCNGC 8 cut(s) 8, 23, 26, 108, 136, 145, 229, 552
Sau3AI GATC 5 cut(s) 222, 405, 450, 519, 561
Sau96I GGNCC 4 cut(s) 244, 349, 379, 436
ScrFI CCNGG 1 cut(s) 279
SetI ASST 8 cut(s) 101, 159, 246, 287, 424, 553, 663, 702
SfcI CTRYAG 2 cut(s) 486, 552
Sfr303I CCGCGG 1 cut(s) 339
SgrBI CCGCGG 1 cut(s) 339
SinI GGWCC 3 cut(s) 244, 379, 436
SmiMI CAYNNNNRTG 1 cut(s) 624
SpeI ACTAGT 1 cut(s) 665
Sse9I AATT 2 cut(s) 601, 712
SseBI AGGCCT 1 cut(s) 395
SspMI CTAG 2 cut(s) 498, 666
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 277
TaaI ACNGT 3 cut(s) 187, 344, 356
TaiI ACGT 1 cut(s) 702
TaqI TCGA 2 cut(s) 210, 750
TasI AATT 2 cut(s) 601, 712
TauI GCSGC 6 cut(s) 10, 25, 110, 138, 147, 231
TfiI GAWTC 1 cut(s) 640
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TscAI CASTG 2 cut(s) 359, 561
TseFI GTSAC 1 cut(s) 661
TseI GCWGC 2 cut(s) 25, 551
Tsp45I GTSAC 1 cut(s) 661
TspDTI ATGAA 1 cut(s) 176
TspGWI ACGGA 2 cut(s) 502, 613
TspRI CASTG 2 cut(s) 359, 561
Van91I CCANNNNNTGG 1 cut(s) 714
VpaK11BI GGWCC 3 cut(s) 244, 379, 436
XagI CCTNNNNNAGG 1 cut(s) 161
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 746
XspI CTAG 2 cut(s) 498, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.