Rmu_sc0001208.1_g000048

expansin-A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001208.1
OG0010451
Physical Location & Seq
Forward (+)
198917 .. 203659
4743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001208.1_g000048.1.cds

Sequence Viewer

Length: 807 bp
atgtcgccgccaccgacgctaacgccggtgcttctcctcctcctcctattcacaccacccctcctctcccactcgtcttcttcttcttctgcgataaccttcaccgactccccctcaatctacgaatggcagtccgcacgcgccacctactacgcggcctcggaccctcgcgacgcggtgggcggcgcgtgcggctacggcgacctgacgaaggccggtttcggaatggcgacggtggggctgagcgaggcgctgttcgagcgtggccagatctgcggcgcgtgcttcgaggtccgctgcgtggaggacatgcggtggtgcattcccggaacctccatcattttgacggccacgaacttctgcgctcccaactatggcttcaccgccgagggtgggggccactgtaaccctccgaataagcacttggtcctcccgattgaggcctttgagaagatcgctatctggaaagctggtaatatgcccgtccagtatcgcaggataaagtgtagaaaggaaggaggagttcgttttaccatagatgggtctagtatctttatttcagtgctgatcagcaatgttgccggcactggagacataactgcagtgaagatcaagggttcaagaactggttggcttccaatgggacgaaattggggtcaaaactggcatataaatgctgatttgaagaatcagccgctttcttttgaggtcactagcagcgatgggcagactgttacatcttacaatgtcgctcccaagaattggaacttcgggcagacttttgacgggaagcagtttgagtcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

268

Amino Acids

29.02

Weight (kDa)

7.55

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 762
AccII CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
AcoI YGGCCR 2 cut(s) 265, 348
AfiI CCNNNNNNNGG 7 cut(s) 211, 301, 374, 393, 439, 540, 762
AgsI TTSAA 2 cut(s) 621, 685
AjuI GAANNNNNNNTTGG 4 cut(s) 362, 394, 407, 439
AluBI AGCT 1 cut(s) 470
AluI AGCT 1 cut(s) 470
Alw26I GTCTC 1 cut(s) 585
AoxI GGCC 6 cut(s) 156, 213, 265, 348, 397, 441
ApeKI GCWGC 2 cut(s) 297, 717
ArsI GACNNNNNNTTYG 2 cut(s) 640, 672
AspLEI GCGC 5 cut(s) 143, 188, 253, 281, 365
AspS9I GGNCC 4 cut(s) 163, 292, 397, 427
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 94, 373
AvaII GGWCC 3 cut(s) 163, 292, 427
BalI TGGCCA 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 284, 729
BccI CCATC 3 cut(s) 344, 533, 716
BceAI ACGGC 2 cut(s) 214, 363
BclI TGATCA 1 cut(s) 567
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 2 cut(s) 546, 714
BfmI CTRYAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 254
BglII AGATCT 1 cut(s) 270
BisI GCNGC 8 cut(s) 8, 156, 184, 193, 277, 298, 695, 718
BlpI GCTNAGC 1 cut(s) 242
BlsI GCNGC 8 cut(s) 9, 157, 185, 194, 278, 299, 696, 719
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 3 cut(s) 163, 292, 427
BmgT120I GGNCC 4 cut(s) 163, 292, 397, 427
BmiI GGNNCC 3 cut(s) 165, 331, 398
BmrFI CCNGG 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 609
Bpu1102I GCTNAGC 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 327
BsaBI GATNNNNATC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 159, 387
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 7 cut(s) 211, 301, 374, 393, 439, 540, 762
Bse118I RCCGGY 3 cut(s) 25, 215, 581
Bse1I ACTGG 4 cut(s) 487, 592, 631, 668
Bse3DI GCAATG 1 cut(s) 580
Bse8I GATNNNNATC 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 159, 387
BseJI GATNNNNATC 1 cut(s) 458
BseLI CCNNNNNNNGG 7 cut(s) 211, 301, 374, 393, 439, 540, 762
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 4 cut(s) 487, 592, 631, 668
BseRI GAGGAG 5 cut(s) 26, 29, 32, 53, 534
BseXI GCAGC 2 cut(s) 284, 729
Bsh1236I CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
BshFI GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
BsiSI CCGG 4 cut(s) 26, 216, 327, 582
BslFI GGGAC 1 cut(s) 657
BslI CCNNNNNNNGG 7 cut(s) 211, 301, 374, 393, 439, 540, 762
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 1 cut(s) 657
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
Bsp143I GATC 4 cut(s) 270, 453, 567, 609
Bsp1720I GCTNAGC 1 cut(s) 242
Bsp68I TCGCGA 1 cut(s) 171
BspANI GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
BspCNI CTCAG 1 cut(s) 234
BspFNI CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
BspLI GGNNCC 3 cut(s) 165, 331, 398
BspMAI CTGCAG 1 cut(s) 604
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 3 cut(s) 25, 215, 581
BsrI ACTGG 4 cut(s) 487, 592, 631, 668
BssAI RCCGGY 3 cut(s) 25, 215, 581
BssECI CCNNGG 2 cut(s) 159, 387
BssMI GATC 4 cut(s) 270, 453, 567, 609
Bst4CI ACNGT 3 cut(s) 235, 404, 733
BstC8I GCNNGC 4 cut(s) 139, 190, 283, 583
BstDEI CTNAG 1 cut(s) 242
BstENI CCTNNNNNAGG 1 cut(s) 209
BstFNI CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 5 cut(s) 143, 188, 253, 281, 365
BstKTI GATC 4 cut(s) 273, 456, 570, 612
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 4 cut(s) 270, 453, 567, 609
BstMWI GCNNNNNNNGC 7 cut(s) 16, 189, 192, 198, 259, 273, 282
BstNSI RCATGY 1 cut(s) 313
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 600
BstUI CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
BstV1I GCAGC 2 cut(s) 284, 729
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuRI GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
BtgZI GCGATG 1 cut(s) 735
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 4 cut(s) 400, 567, 585, 609
BtuMI TCGCGA 1 cut(s) 171
Cac8I GCNNGC 4 cut(s) 139, 190, 283, 583
CfoI GCGC 5 cut(s) 143, 188, 253, 281, 365
Cfr10I RCCGGY 3 cut(s) 25, 215, 581
Cfr13I GGNCC 4 cut(s) 163, 292, 397, 427
CseI GACGC 2 cut(s) 25, 182
CviAII CATG 1 cut(s) 310
DdeI CTNAG 1 cut(s) 242
DpnI GATC 4 cut(s) 272, 455, 569, 611
DpnII GATC 4 cut(s) 270, 453, 567, 609
EaeI YGGCCR 2 cut(s) 265, 348
Eco147I AGGCCT 1 cut(s) 443
Eco47I GGWCC 3 cut(s) 163, 292, 427
EcoNI CCTNNNNNAGG 1 cut(s) 209
FaeI CATG 1 cut(s) 313
FaiI YATR 7 cut(s) 311, 375, 479, 536, 596, 669, 671
FaqI GGGAC 1 cut(s) 657
FatI CATG 1 cut(s) 309
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 8 cut(s) 8, 156, 184, 193, 277, 298, 695, 718
Fsp4HI GCNGC 8 cut(s) 8, 156, 184, 193, 277, 298, 695, 718
FspBI CTAG 2 cut(s) 546, 714
GlaI GCGC 5 cut(s) 142, 187, 252, 280, 364
GluI GCNGC 8 cut(s) 8, 156, 184, 193, 277, 298, 695, 718
GsuI CTGGAG 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
HapII CCGG 4 cut(s) 26, 216, 327, 582
HgaI GACGC 2 cut(s) 25, 182
HhaI GCGC 5 cut(s) 143, 188, 253, 281, 365
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 5 cut(s) 141, 186, 251, 279, 363
HinP1I GCGC 5 cut(s) 141, 186, 251, 279, 363
HinfI GANTC 3 cut(s) 107, 688, 800
HpaII CCGG 4 cut(s) 26, 216, 327, 582
HphI GGTGA 2 cut(s) 94, 373
Hpy188I TCNGA 3 cut(s) 163, 224, 414
Hpy188III TCNNGA 5 cut(s) 170, 433, 463, 621, 804
Hpy99I CGWCG 3 cut(s) 19, 176, 235
HpyAV CCTTC 3 cut(s) 109, 205, 509
HpyCH4III ACNGT 3 cut(s) 235, 404, 733
HpyCH4V TGCA 2 cut(s) 321, 602
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 189, 192, 198, 259, 273, 282
HpyF3I CTNAG 1 cut(s) 242
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 5 cut(s) 141, 186, 251, 279, 363
KroI GCCGGC 1 cut(s) 581
KroNI GCCGGC 1 cut(s) 583
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 4 cut(s) 270, 453, 567, 609
LmnI GCTCC 2 cut(s) 370, 757
Lsp1109I GCAGC 2 cut(s) 284, 729
MaeI CTAG 2 cut(s) 546, 714
MaeIII GTNAC 3 cut(s) 404, 709, 733
MalI GATC 4 cut(s) 272, 455, 569, 611
MboI GATC 4 cut(s) 270, 453, 567, 609
MboII GAAGA 7 cut(s) 69, 72, 75, 78, 463, 619, 697
MflI RGATCY 1 cut(s) 270
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 2 cut(s) 649, 760
MluNI TGGCCA 1 cut(s) 267
MlyI GAGTC 1 cut(s) 101
Mox20I TGGCCA 1 cut(s) 267
MroNI GCCGGC 1 cut(s) 581
MscI TGGCCA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 672
Msp20I TGGCCA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 4 cut(s) 26, 216, 327, 582
MspR9I CCNGG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 321
MvnI CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
MwoI GCNNNNNNNGC 7 cut(s) 16, 189, 192, 198, 259, 273, 282
NaeI GCCGGC 1 cut(s) 583
NciI CCSGG 1 cut(s) 327
NdeII GATC 4 cut(s) 270, 453, 567, 609
NgoMIV GCCGGC 1 cut(s) 581
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 3 cut(s) 165, 331, 398
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 1 cut(s) 709
NruI TCGCGA 1 cut(s) 171
NspI RCATGY 1 cut(s) 313
PceI AGGCCT 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 1 cut(s) 321
PdiI GCCGGC 1 cut(s) 583
PfeI GAWTC 1 cut(s) 688
PflMI CCANNNNNTGG 1 cut(s) 762
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 8 cut(s) 9, 157, 185, 194, 278, 299, 696, 719
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PspN4I GGNNCC 3 cut(s) 165, 331, 398
PspPI GGNCC 4 cut(s) 163, 292, 397, 427
PstI CTGCAG 1 cut(s) 604
PsuI RGATCY 1 cut(s) 270
RruI TCGCGA 1 cut(s) 171
RseI CAYNNNNRTG 1 cut(s) 672
SatI GCNGC 8 cut(s) 8, 156, 184, 193, 277, 298, 695, 718
Sau3AI GATC 4 cut(s) 270, 453, 567, 609
Sau96I GGNCC 4 cut(s) 163, 292, 397, 427
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 327
SetI ASST 7 cut(s) 101, 149, 207, 294, 335, 472, 711
SfcI CTRYAG 1 cut(s) 600
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 3 cut(s) 163, 292, 427
SmiMI CAYNNNNRTG 1 cut(s) 672
Sse9I AATT 2 cut(s) 649, 760
SseBI AGGCCT 1 cut(s) 443
SspMI CTAG 2 cut(s) 546, 714
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 1 cut(s) 325
TaaI ACNGT 3 cut(s) 235, 404, 733
TaqI TCGA 2 cut(s) 258, 288
TasI AATT 2 cut(s) 649, 760
TauI GCSGC 6 cut(s) 10, 158, 186, 195, 279, 697
TfiI GAWTC 1 cut(s) 688
TscAI CASTG 4 cut(s) 407, 567, 592, 609
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 2 cut(s) 297, 717
Tsp45I GTSAC 1 cut(s) 709
TspRI CASTG 4 cut(s) 407, 567, 592, 609
Van91I CCANNNNNTGG 1 cut(s) 762
VpaK11BI GGWCC 3 cut(s) 163, 292, 427
XagI CCTNNNNNAGG 1 cut(s) 209
XceI RCATGY 1 cut(s) 313
XspI CTAG 2 cut(s) 546, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.