Rw3G025670

expansin-A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010451
Physical Location & Seq
Reverse (-)
34486240 .. 34491460
5221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025670.1

Sequence Viewer

Length: 819 bp
ATGTCGCCACCACCGACGCTAACGCCGGTGCTCCTCCTCCTCCTCCTCCTATTCACACCACCCCTCCTCTCCCACTCGCCTTCTTCTTCTTCTTCTTCTTCTGCGATAACCTTCACCGACTCCCCCTCAATCTACGAATGGCAGTCCGCACGCGCCACCTACTACGCCGCCTCCGACCCTCGCGACGCGGTGGGCGGCGCGTGCGGCTACGGCGACCTGACGAAGGCCGGTTTCGGAATGGCGACGGTGGGGCTGAGCGAGGCGCTGTTCGAGCGTGGCCAGATCTGCGGCGCGTGCTTCGAGGTCCGCTGCGTGGAGGACATGCGGTGGTGCATTCCCGGAACCTCCATCATTTTGACGGCCACGAACTTCTGCGCTCCCAACTATGGCTTCACCGCCGAGGGTGGGGGCCACTGTAACCCTCCGAATAAGCACTTGGTCCTCCCGATTGAGGCCTTTGAGAAGATCGCTATCTGGAAAGCTGGTAATATGCCCGTCCAGTATCGCAGGATAAAGTGTAGAAAGGAAGGAGGAGTTCGTTTTACCATAGATGGGTCTAGTATCTTTATTTCAGTGCTGATCAGCAATGTTGCCGGCACTGGAGACATAAGTGCAGTGAAGATCAAGGGTTCAAGAACTGGTTGGCTTCCAATGGGACGAAATTGGGGTCAAAACTGGCATATAAATGCTGATTTGAAGAATCAGCCGCTTTCTTTTGAGGTCACTAGCAGCGATGGGCAGACAGTTACATCTTACAATGTCGCTCCCAAGAATTGGAACTTCGGGCAGACTTTTGACGGGAAGCAGTTTGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

272

Amino Acids

29.39

Weight (kDa)

7.55

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 83 - 168 6.7e-17 Lytic transglycolase
Expansin_C PF01357 179 - 256 5.5e-30 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 774
AccII CGCG 5 cut(s) 153, 183, 188, 200, 293
AcoI YGGCCR 2 cut(s) 277, 360
AfiI CCNNNNNNNGG 7 cut(s) 223, 313, 386, 405, 451, 552, 774
AgsI TTSAA 2 cut(s) 633, 697
AjuI GAANNNNNNNTTGG 4 cut(s) 374, 406, 419, 451
AluBI AGCT 1 cut(s) 482
AluI AGCT 1 cut(s) 482
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 597
AoxI GGCC 5 cut(s) 225, 277, 360, 409, 453
ApeKI GCWGC 2 cut(s) 309, 729
ArsI GACNNNNNNTTYG 2 cut(s) 652, 684
AspLEI GCGC 5 cut(s) 155, 200, 265, 293, 377
AspS9I GGNCC 3 cut(s) 304, 409, 439
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 2 cut(s) 106, 385
AvaII GGWCC 2 cut(s) 304, 439
BalI TGGCCA 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 296, 741
BccI CCATC 3 cut(s) 356, 545, 728
BceAI ACGGC 2 cut(s) 226, 375
BclI TGATCA 1 cut(s) 579
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 2 cut(s) 558, 726
BfoI RGCGCY 1 cut(s) 266
BglII AGATCT 1 cut(s) 282
BisI GCNGC 7 cut(s) 168, 196, 205, 289, 310, 707, 730
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 7 cut(s) 169, 197, 206, 290, 311, 708, 731
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 2 cut(s) 304, 439
BmgT120I GGNCC 3 cut(s) 304, 409, 439
BmiI GGNNCC 2 cut(s) 343, 410
BmrFI CCNGG 1 cut(s) 339
BpmI CTGGAG 1 cut(s) 621
Bpu1102I GCTNAGC 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 399
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 155, 185
Bsc4I CCNNNNNNNGG 7 cut(s) 223, 313, 386, 405, 451, 552, 774
Bse118I RCCGGY 3 cut(s) 25, 227, 593
Bse1I ACTGG 4 cut(s) 499, 604, 643, 680
Bse3DI GCAATG 1 cut(s) 592
Bse8I GATNNNNATC 1 cut(s) 470
BseDI CCNNGG 1 cut(s) 399
BseJI GATNNNNATC 1 cut(s) 470
BseLI CCNNNNNNNGG 7 cut(s) 223, 313, 386, 405, 451, 552, 774
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 1 cut(s) 245
BseNI ACTGG 4 cut(s) 499, 604, 643, 680
BseRI GAGGAG 7 cut(s) 23, 26, 29, 32, 35, 56, 546
BseXI GCAGC 2 cut(s) 296, 741
BsgI GTGCAG 1 cut(s) 633
Bsh1236I CGCG 5 cut(s) 153, 183, 188, 200, 293
BshFI GGCC 5 cut(s) 227, 279, 362, 411, 455
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 4 cut(s) 26, 228, 339, 594
BslFI GGGAC 1 cut(s) 669
BslI CCNNNNNNNGG 7 cut(s) 223, 313, 386, 405, 451, 552, 774
BsmAI GTCTC 1 cut(s) 597
BsmFI GGGAC 1 cut(s) 669
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 5 cut(s) 227, 279, 362, 411, 455
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 4 cut(s) 282, 465, 579, 621
Bsp1720I GCTNAGC 1 cut(s) 254
Bsp68I TCGCGA 1 cut(s) 183
BspANI GGCC 5 cut(s) 227, 279, 362, 411, 455
BspCNI CTCAG 1 cut(s) 246
BspFNI CGCG 5 cut(s) 153, 183, 188, 200, 293
BspLI GGNNCC 2 cut(s) 343, 410
BsrDI GCAATG 1 cut(s) 592
BsrFI RCCGGY 3 cut(s) 25, 227, 593
BsrI ACTGG 4 cut(s) 499, 604, 643, 680
BssAI RCCGGY 3 cut(s) 25, 227, 593
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 4 cut(s) 282, 465, 579, 621
Bst4CI ACNGT 3 cut(s) 247, 416, 745
BstC8I GCNNGC 4 cut(s) 151, 202, 295, 595
BstDEI CTNAG 1 cut(s) 254
BstENI CCTNNNNNAGG 1 cut(s) 221
BstFNI CGCG 5 cut(s) 153, 183, 188, 200, 293
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 5 cut(s) 155, 200, 265, 293, 377
BstKTI GATC 4 cut(s) 285, 468, 582, 624
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 4 cut(s) 282, 465, 579, 621
BstMWI GCNNNNNNNGC 6 cut(s) 201, 204, 210, 271, 285, 294
BstNSI RCATGY 1 cut(s) 325
BstSCI CCNGG 1 cut(s) 337
BstUI CGCG 5 cut(s) 153, 183, 188, 200, 293
BstV1I GCAGC 2 cut(s) 296, 741
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BsuRI GGCC 5 cut(s) 227, 279, 362, 411, 455
BtgZI GCGATG 1 cut(s) 747
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 4 cut(s) 412, 579, 597, 621
BtuMI TCGCGA 1 cut(s) 183
Cac8I GCNNGC 4 cut(s) 151, 202, 295, 595
CfoI GCGC 5 cut(s) 155, 200, 265, 293, 377
Cfr10I RCCGGY 3 cut(s) 25, 227, 593
Cfr13I GGNCC 3 cut(s) 304, 409, 439
CseI GACGC 2 cut(s) 25, 194
CviAII CATG 1 cut(s) 322
DdeI CTNAG 1 cut(s) 254
DpnI GATC 4 cut(s) 284, 467, 581, 623
DpnII GATC 4 cut(s) 282, 465, 579, 621
EaeI YGGCCR 2 cut(s) 277, 360
Eco147I AGGCCT 1 cut(s) 455
Eco47I GGWCC 2 cut(s) 304, 439
EcoNI CCTNNNNNAGG 1 cut(s) 221
FaeI CATG 1 cut(s) 325
FaiI YATR 7 cut(s) 323, 387, 491, 548, 608, 681, 683
FaqI GGGAC 1 cut(s) 669
FatI CATG 1 cut(s) 321
FbaI TGATCA 1 cut(s) 579
Fnu4HI GCNGC 7 cut(s) 168, 196, 205, 289, 310, 707, 730
Fsp4HI GCNGC 7 cut(s) 168, 196, 205, 289, 310, 707, 730
FspBI CTAG 2 cut(s) 558, 726
GlaI GCGC 5 cut(s) 154, 199, 264, 292, 376
GluI GCNGC 7 cut(s) 168, 196, 205, 289, 310, 707, 730
GsuI CTGGAG 1 cut(s) 621
HaeII RGCGCY 1 cut(s) 266
HaeIII GGCC 5 cut(s) 227, 279, 362, 411, 455
HapII CCGG 4 cut(s) 26, 228, 339, 594
HgaI GACGC 2 cut(s) 25, 194
HhaI GCGC 5 cut(s) 155, 200, 265, 293, 377
Hin1II CATG 1 cut(s) 325
Hin6I GCGC 5 cut(s) 153, 198, 263, 291, 375
HinP1I GCGC 5 cut(s) 153, 198, 263, 291, 375
HinfI GANTC 3 cut(s) 119, 700, 812
HpaII CCGG 4 cut(s) 26, 228, 339, 594
HphI GGTGA 2 cut(s) 106, 385
Hpy188I TCNGA 3 cut(s) 175, 236, 426
Hpy188III TCNNGA 5 cut(s) 182, 445, 475, 633, 816
Hpy99I CGWCG 3 cut(s) 19, 188, 247
HpyAV CCTTC 4 cut(s) 90, 121, 217, 521
HpyCH4III ACNGT 3 cut(s) 247, 416, 745
HpyCH4V TGCA 2 cut(s) 333, 614
HpyF10VI GCNNNNNNNGC 6 cut(s) 201, 204, 210, 271, 285, 294
HpyF3I CTNAG 1 cut(s) 254
Hsp92II CATG 1 cut(s) 325
HspAI GCGC 5 cut(s) 153, 198, 263, 291, 375
KroI GCCGGC 1 cut(s) 593
KroNI GCCGGC 1 cut(s) 595
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 4 cut(s) 282, 465, 579, 621
LmnI GCTCC 3 cut(s) 36, 382, 769
Lsp1109I GCAGC 2 cut(s) 296, 741
MaeI CTAG 2 cut(s) 558, 726
MaeIII GTNAC 3 cut(s) 416, 721, 745
MalI GATC 4 cut(s) 284, 467, 581, 623
MboI GATC 4 cut(s) 282, 465, 579, 621
MboII GAAGA 9 cut(s) 75, 78, 81, 84, 87, 90, 475, 631, 709
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 2 cut(s) 661, 772
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 198
Mox20I TGGCCA 1 cut(s) 279
MroNI GCCGGC 1 cut(s) 593
MscI TGGCCA 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 684
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 4 cut(s) 26, 228, 339, 594
MspR9I CCNGG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 333
MvnI CGCG 5 cut(s) 153, 183, 188, 200, 293
MwoI GCNNNNNNNGC 6 cut(s) 201, 204, 210, 271, 285, 294
NaeI GCCGGC 1 cut(s) 595
NciI CCSGG 1 cut(s) 339
NdeII GATC 4 cut(s) 282, 465, 579, 621
NgoMIV GCCGGC 1 cut(s) 593
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 2 cut(s) 343, 410
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 721
NruI TCGCGA 1 cut(s) 183
NspI RCATGY 1 cut(s) 325
PceI AGGCCT 1 cut(s) 455
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 171
PctI GAATGC 1 cut(s) 333
PdiI GCCGGC 1 cut(s) 595
PfeI GAWTC 1 cut(s) 700
PflMI CCANNNNNTGG 1 cut(s) 774
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 7 cut(s) 169, 197, 206, 290, 311, 708, 731
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PspN4I GGNNCC 2 cut(s) 343, 410
PspPI GGNCC 3 cut(s) 304, 409, 439
PsuI RGATCY 1 cut(s) 282
RruI TCGCGA 1 cut(s) 183
RseI CAYNNNNRTG 1 cut(s) 684
SatI GCNGC 7 cut(s) 168, 196, 205, 289, 310, 707, 730
Sau3AI GATC 4 cut(s) 282, 465, 579, 621
Sau96I GGNCC 3 cut(s) 304, 409, 439
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 33
SetI ASST 7 cut(s) 113, 161, 219, 306, 347, 484, 723
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 2 cut(s) 304, 439
SmiMI CAYNNNNRTG 1 cut(s) 684
Sse9I AATT 2 cut(s) 661, 772
SseBI AGGCCT 1 cut(s) 455
SspMI CTAG 2 cut(s) 558, 726
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 1 cut(s) 337
TaaI ACNGT 3 cut(s) 247, 416, 745
TaqI TCGA 2 cut(s) 270, 300
TasI AATT 2 cut(s) 661, 772
TauI GCSGC 5 cut(s) 170, 198, 207, 291, 709
TfiI GAWTC 1 cut(s) 700
TscAI CASTG 4 cut(s) 419, 579, 604, 621
TseFI GTSAC 1 cut(s) 721
TseI GCWGC 2 cut(s) 309, 729
Tsp45I GTSAC 1 cut(s) 721
TspRI CASTG 4 cut(s) 419, 579, 604, 621
Van91I CCANNNNNTGG 1 cut(s) 774
VpaK11BI GGWCC 2 cut(s) 304, 439
XagI CCTNNNNNAGG 1 cut(s) 221
XceI RCATGY 1 cut(s) 325
XspI CTAG 2 cut(s) 558, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.