pycom09g18760

expansin-A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010451
Physical Location & Seq
Forward (+)
19530573 .. 19532546
1974 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g18760.2

Sequence Viewer

Length: 954 bp
ATGCAAAATGTTTTCTCTTTCCGTCTTCACCTGTACTCTGTCTCTCTGGATCGATCTCTCACTCACTCATTCACACTAATCCTTCCTCGAACGAGAAAGCAAATTAATAACGCCGACGACAAAAAGCCGACGGCGTTTAAACAAAGAACGGCGGAAATGTCGCCGCCAGCTCTCGCCCTGCTCCTCCTCCTCCCACTCGCGCTCGCCTCCACCGCCCTCTCCCACTTCCACTCCTCCTCCTCCTTCCCCACCGCCTCCCCCTCCCTCTACGACTGGACCTCCGCACGTGCCACCTACTACGCCGCCGCCGACCCCCGCGACAAGGTTGGGGGCGCGTGCGGGTACGGCGACCTCGAGAAGGCCGGGTATGGGCTGGCGACGGTGGGGCTGAGCGAGGCGCTGTTCGAGCGGGGCCAGATCTGCGGCGCGTGCTTCGAGGTCCGGTGCGTGGAGGACATGCGGTGGTGCATTCCTGGGACGTCGATTATCGTGACGGCGACCAACTTCTGCCCGCCGAATTACGGGTTCACGGCGGAAGGCGGGGGCCACTGCAACGCGCCGAACAAGCATTTGGTGCTTCCCATCGAGGCGTTTGAGAAGATCGCTATTTGGAAGGCTGGCAATATGCCCGTCCAGTATCGTAGGATCAAGTGTAGAAAGGAAGGAGGAATTCGTTTCACCATAGATGGTTCTAATATCTTTATCTCAGTGCTCATCAGCAATGTGGCTGGTGCTGGAGACATTGTTGCGGTGAAGATCAAGGGTTCAAGAACCGGCTGGCTTCCAATGGGACGGAATTGGGGCCAAAACTGGCATGCAAGCGCCGATTTACTGAACCAACCTCTTTCTTTCGAGGTCACAAGCAGCGATGGGCGCACAGTTACATCTTACAACATTGCTCCCAAGTCTTGGAACTTTGGGCAGACTTTTGAAGGGAAGCAGTTTGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

318

Amino Acids

34.66

Weight (kDa)

8.98

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 482
AccB7I CCANNNNNTGG 1 cut(s) 909
AccBSI CCGCTC 1 cut(s) 409
AccII CGCG 5 cut(s) 200, 318, 335, 428, 557
AclWI GGATC 2 cut(s) 57, 653
AcsI RAATTY 1 cut(s) 669
AcvI CACGTG 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 2 cut(s) 35, 344
AfiI CCNNNNNNNGG 6 cut(s) 322, 358, 369, 448, 521, 909
AgsI TTSAA 2 cut(s) 768, 932
AjnI CCWGG 1 cut(s) 472
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 2 cut(s) 46, 732
AlwI GGATC 2 cut(s) 57, 653
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 4 cut(s) 360, 412, 544, 802
ApeKI GCWGC 1 cut(s) 864
ApoI RAATTY 1 cut(s) 669
AseI ATTAAT 1 cut(s) 105
AspLEI GCGC 7 cut(s) 202, 335, 400, 428, 559, 824, 876
AspS9I GGNCC 5 cut(s) 276, 412, 439, 544, 802
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 3 cut(s) 20, 670, 763
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 2 cut(s) 276, 439
BbrPI CACGTG 1 cut(s) 287
BbsI GAAGAC 1 cut(s) 17
Bbv12I GWGCWC 1 cut(s) 714
BbvI GCAGC 1 cut(s) 876
BccI CCATC 3 cut(s) 590, 680, 863
BceAI ACGGC 5 cut(s) 147, 165, 361, 510, 546
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 1 cut(s) 364
BcoDI GTCTC 2 cut(s) 46, 732
BfoI RGCGCY 2 cut(s) 401, 825
BglII AGATCT 1 cut(s) 417
BisI GCNGC 5 cut(s) 164, 303, 306, 424, 865
BlpI GCTNAGC 1 cut(s) 389
BlsI GCNGC 5 cut(s) 165, 304, 307, 425, 866
Bme1390I CCNGG 2 cut(s) 364, 474
Bme18I GGWCC 2 cut(s) 276, 439
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 5 cut(s) 276, 412, 439, 544, 802
BmiI GGNNCC 3 cut(s) 413, 545, 803
BmrFI CCNGG 2 cut(s) 364, 474
BpiI GAAGAC 1 cut(s) 17
BpmI CTGGAG 1 cut(s) 756
Bpu1102I GCTNAGC 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 364
Bsa29I ATCGAT 1 cut(s) 52
BsaAI YACGTR 1 cut(s) 287
BsaHI GRCGYC 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 473
BsaWI WCCGGW 1 cut(s) 441
BsaXI ACNNNNNCTCC 8 cut(s) 215, 221, 245, 251, 263, 293, 729, 759
Bsc4I CCNNNNNNNGG 6 cut(s) 322, 358, 369, 448, 521, 909
Bse118I RCCGGY 1 cut(s) 773
Bse1I ACTGG 3 cut(s) 278, 634, 815
Bse3DI GCAATG 2 cut(s) 727, 894
BseBI CCWGG 1 cut(s) 474
BseCI ATCGAT 1 cut(s) 52
BseDI CCNNGG 1 cut(s) 473
BseLI CCNNNNNNNGG 6 cut(s) 322, 358, 369, 448, 521, 909
BseMI GCAATG 2 cut(s) 727, 894
BseMII CTCAG 2 cut(s) 380, 720
BseNI ACTGG 3 cut(s) 278, 634, 815
BseRI GAGGAG 6 cut(s) 173, 176, 179, 223, 226, 229
BseXI GCAGC 1 cut(s) 876
Bsh1236I CGCG 5 cut(s) 200, 318, 335, 428, 557
BshFI GGCC 4 cut(s) 362, 414, 546, 804
BshVI ATCGAT 1 cut(s) 52
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 3 cut(s) 363, 442, 774
BslFI GGGAC 2 cut(s) 490, 804
BslI CCNNNNNNNGG 6 cut(s) 322, 358, 369, 448, 521, 909
BsmAI GTCTC 2 cut(s) 46, 732
BsmFI GGGAC 2 cut(s) 490, 804
BsmI GAATGC 1 cut(s) 468
BsnI GGCC 4 cut(s) 362, 414, 546, 804
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 714
Bsp143I GATC 6 cut(s) 49, 53, 417, 600, 645, 756
Bsp1720I GCTNAGC 1 cut(s) 389
BspANI GGCC 4 cut(s) 362, 414, 546, 804
BspCNI CTCAG 2 cut(s) 381, 719
BspDI ATCGAT 1 cut(s) 52
BspFNI CGCG 5 cut(s) 200, 318, 335, 428, 557
BspLI GGNNCC 3 cut(s) 413, 545, 803
BspPI GGATC 2 cut(s) 57, 653
BsrBI CCGCTC 1 cut(s) 409
BsrDI GCAATG 2 cut(s) 727, 894
BsrFI RCCGGY 1 cut(s) 773
BsrI ACTGG 3 cut(s) 278, 634, 815
BssAI RCCGGY 1 cut(s) 773
BssECI CCNNGG 1 cut(s) 473
BssMI GATC 6 cut(s) 49, 53, 417, 600, 645, 756
BssNI GRCGYC 1 cut(s) 479
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 2 cut(s) 382, 880
BstACI GRCGYC 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 574
BstBAI YACGTR 1 cut(s) 287
BstDEI CTNAG 2 cut(s) 389, 706
BstENI CCTNNNNNAGG 1 cut(s) 356
BstFNI CGCG 5 cut(s) 200, 318, 335, 428, 557
BstH2I RGCGCY 2 cut(s) 401, 825
BstHHI GCGC 7 cut(s) 202, 335, 400, 428, 559, 824, 876
BstKTI GATC 6 cut(s) 52, 56, 420, 603, 648, 759
BstMAI GTCTC 2 cut(s) 46, 732
BstMBI GATC 6 cut(s) 49, 53, 417, 600, 645, 756
BstMWI GCNNNNNNNGC 8 cut(s) 212, 345, 406, 420, 429, 565, 574, 873
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 2 cut(s) 460, 818
BstSCI CCNGG 2 cut(s) 362, 472
BstUI CGCG 5 cut(s) 200, 318, 335, 428, 557
BstV1I GCAGC 1 cut(s) 876
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
Bsu15I ATCGAT 1 cut(s) 52
BsuRI GGCC 4 cut(s) 362, 414, 546, 804
BsuTUI ATCGAT 1 cut(s) 52
BtgZI GCGATG 1 cut(s) 882
BtsI GCAGTG 1 cut(s) 547
BtsIMutI CAGTG 2 cut(s) 547, 714
CfoI GCGC 7 cut(s) 202, 335, 400, 428, 559, 824, 876
Cfr10I RCCGGY 1 cut(s) 773
Cfr13I GGNCC 5 cut(s) 276, 412, 439, 544, 802
ClaI ATCGAT 1 cut(s) 52
Csp6I GTAC 2 cut(s) 34, 343
CviAII CATG 2 cut(s) 457, 815
CviQI GTAC 2 cut(s) 34, 343
DdeI CTNAG 2 cut(s) 389, 706
DpnI GATC 6 cut(s) 51, 55, 419, 602, 647, 758
DpnII GATC 6 cut(s) 49, 53, 417, 600, 645, 756
DraI TTTAAA 1 cut(s) 139
EciI GGCGGA 2 cut(s) 167, 548
Eco47I GGWCC 2 cut(s) 276, 439
Eco72I CACGTG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 353
EcoNI CCTNNNNNAGG 1 cut(s) 356
EcoRI GAATTC 1 cut(s) 669
EcoRII CCWGG 1 cut(s) 472
FaeI CATG 2 cut(s) 460, 818
FaiI YATR 5 cut(s) 369, 458, 626, 683, 816
FaqI GGGAC 2 cut(s) 490, 804
FatI CATG 2 cut(s) 456, 814
FauI CCCGC 5 cut(s) 323, 332, 402, 519, 533
Fnu4HI GCNGC 5 cut(s) 164, 303, 306, 424, 865
Fsp4HI GCNGC 5 cut(s) 164, 303, 306, 424, 865
GlaI GCGC 7 cut(s) 201, 334, 399, 427, 558, 823, 875
GluI GCNGC 5 cut(s) 164, 303, 306, 424, 865
GsuI CTGGAG 1 cut(s) 756
HaeII RGCGCY 2 cut(s) 401, 825
HaeIII GGCC 4 cut(s) 362, 414, 546, 804
HapII CCGG 3 cut(s) 363, 442, 774
HhaI GCGC 7 cut(s) 202, 335, 400, 428, 559, 824, 876
Hin1I GRCGYC 1 cut(s) 479
Hin1II CATG 2 cut(s) 460, 818
Hin6I GCGC 7 cut(s) 200, 333, 398, 426, 557, 822, 874
HinP1I GCGC 7 cut(s) 200, 333, 398, 426, 557, 822, 874
HinfI GANTC 1 cut(s) 947
HpaII CCGG 3 cut(s) 363, 442, 774
HphI GGTGA 3 cut(s) 20, 670, 763
Hpy166II GTNNAC 1 cut(s) 528
Hpy188III TCNNGA 4 cut(s) 47, 355, 490, 768
Hpy8I GTNNAC 1 cut(s) 528
Hpy99I CGWCG 4 cut(s) 119, 133, 382, 484
HpyAV CCTTC 7 cut(s) 92, 253, 352, 530, 607, 656, 926
HpyCH4III ACNGT 2 cut(s) 382, 880
HpyCH4IV ACGT 2 cut(s) 286, 479
HpyCH4V TGCA 4 cut(s) 4, 468, 552, 818
HpyF10VI GCNNNNNNNGC 8 cut(s) 212, 345, 406, 420, 429, 565, 574, 873
HpyF3I CTNAG 2 cut(s) 389, 706
HpySE526I ACGT 2 cut(s) 286, 479
Hsp92I GRCGYC 1 cut(s) 479
Hsp92II CATG 2 cut(s) 460, 818
HspAI GCGC 7 cut(s) 200, 333, 398, 426, 557, 822, 874
Kzo9I GATC 6 cut(s) 49, 53, 417, 600, 645, 756
LmnI GCTCC 2 cut(s) 186, 904
Lsp1109I GCAGC 1 cut(s) 876
MaeII ACGT 2 cut(s) 286, 479
MaeIII GTNAC 3 cut(s) 490, 856, 880
MalI GATC 6 cut(s) 51, 55, 419, 602, 647, 758
MbiI CCGCTC 1 cut(s) 409
MboI GATC 6 cut(s) 49, 53, 417, 600, 645, 756
MboII GAAGA 3 cut(s) 17, 610, 766
MflI RGATCY 1 cut(s) 417
MhlI GDGCHC 1 cut(s) 714
MluCI AATT 4 cut(s) 102, 517, 669, 796
MseI TTAA 3 cut(s) 105, 138, 952
MspI CCGG 3 cut(s) 363, 442, 774
MspR9I CCNGG 2 cut(s) 364, 474
MssI GTTTAAAC 1 cut(s) 139
Mva1269I GAATGC 1 cut(s) 468
MvaI CCWGG 1 cut(s) 474
MvnI CGCG 5 cut(s) 200, 318, 335, 428, 557
MwoI GCNNNNNNNGC 8 cut(s) 212, 345, 406, 420, 429, 565, 574, 873
NciI CCSGG 1 cut(s) 364
NdeII GATC 6 cut(s) 49, 53, 417, 600, 645, 756
NlaIII CATG 2 cut(s) 460, 818
NlaIV GGNNCC 3 cut(s) 413, 545, 803
NmuCI GTSAC 2 cut(s) 490, 856
NspI RCATGY 2 cut(s) 460, 818
PaeI GCATGC 1 cut(s) 818
PaeR7I CTCGAG 1 cut(s) 353
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 351
PctI GAATGC 1 cut(s) 468
PfeI GAWTC 1 cut(s) 947
PflMI CCANNNNNTGG 1 cut(s) 909
PkrI GCNGC 5 cut(s) 165, 304, 307, 425, 866
PmaCI CACGTG 1 cut(s) 287
PmeI GTTTAAAC 1 cut(s) 139
PmlI CACGTG 1 cut(s) 287
Ppu21I YACGTR 1 cut(s) 287
PshBI ATTAAT 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 472
PspCI CACGTG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 3 cut(s) 413, 545, 803
PspPI GGNCC 5 cut(s) 276, 412, 439, 544, 802
PsuI RGATCY 1 cut(s) 417
RsaI GTAC 2 cut(s) 35, 344
RsaNI GTAC 2 cut(s) 34, 343
SaqAI TTAA 3 cut(s) 105, 138, 952
SatI GCNGC 5 cut(s) 164, 303, 306, 424, 865
Sau3AI GATC 6 cut(s) 49, 53, 417, 600, 645, 756
Sau96I GGNCC 5 cut(s) 276, 412, 439, 544, 802
ScrFI CCNGG 2 cut(s) 364, 474
SduI GDGCHC 1 cut(s) 714
Sfr274I CTCGAG 1 cut(s) 353
SinI GGWCC 2 cut(s) 276, 439
SlaI CTCGAG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 818
Sse9I AATT 4 cut(s) 102, 517, 669, 796
StyD4I CCNGG 2 cut(s) 362, 472
TaaI ACNGT 2 cut(s) 382, 880
TaiI ACGT 2 cut(s) 289, 482
TaqI TCGA 8 cut(s) 52, 88, 354, 405, 435, 482, 585, 852
TasI AATT 4 cut(s) 102, 517, 669, 796
TatI WGTACW 1 cut(s) 33
TauI GCSGC 4 cut(s) 166, 305, 308, 426
TfiI GAWTC 1 cut(s) 947
Tru1I TTAA 3 cut(s) 105, 138, 952
Tru9I TTAA 3 cut(s) 105, 138, 952
TscAI CASTG 2 cut(s) 554, 714
TseFI GTSAC 2 cut(s) 490, 856
TseI GCWGC 1 cut(s) 864
Tsp45I GTSAC 2 cut(s) 490, 856
TspGWI ACGGA 2 cut(s) 11, 808
TspRI CASTG 2 cut(s) 554, 714
Van91I CCANNNNNTGG 1 cut(s) 909
VpaK11BI GGWCC 2 cut(s) 276, 439
VspI ATTAAT 1 cut(s) 105
XagI CCTNNNNNAGG 1 cut(s) 356
XapI RAATTY 1 cut(s) 669
XceI RCATGY 2 cut(s) 460, 818
XhoI CTCGAG 1 cut(s) 353
ZraI GACGTC 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.