RLG00000022922

expansin-A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010451
Physical Location & Seq
Forward (+)
15204338 .. 15209194
4857 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022922

Sequence Viewer

Length: 804 bp
ATGTCGAAACCGCCGACGCTAACGGCGGTGCTCCTCCTTCTCCTGCTATTCACACCACCCCTCCTCTGCCACTCGCCTTCTTCTTCTGCGATAACCTTCACCGACTCCCCCTCAATCTACGAATGGCAGTCCGCACGCGCCACCTACTACGCCGCCTCCGACCCTCGCGACGCGGTGGGCGGCGCGTGCGGGTACGGCGACCTGGTGAAGGCCGGTTTCGGAATGGCGACTGTGGGGCTGAGCGAGGCGCTGTTCGAGCGTGGCCAGATCTGCGGCGCGTGCTTCGAGGTCCGCTGCGTCGAGGACATGCGGTGGTGCATTCCCGGAACCTCCATCATTTTGACGGCCACGAACTTCTGCGCTCCCAACTATGGCTTCACCGCCGAGGGTGGGGGCCACTGTAACCCTCCGAATAAGCACTTGGTCCTCCCGATTGAGGCCTTTGAGAAGATCGCTATCTGGAAAGCTGGTAATATGCCCGTCCAGTATCGCAGGATAAAGTGTAGAAAGGAAGGAGGAGTTCGTTTTACCATAGATGGGTCTAGTATCTTTATTTCAGTGCTGATCAGCAATGTTGCCGGCACTGGAGACATAACTGCAGTGAAGATTAAGGGTTCAAGAACTGGTTGGCTTCCAATGGGACGAAATTGGGGTCAAAACTGGCATATAAATGCTGATTTGAAGAATCAGCCTCTTTCTTTTGAGGTCACTAGCAGCGATGGGCAGACAGTTACATCTTACAATGTCGCTCCCAAGAATTGGAACTTTGGGCAGAGTTTTGAAGGGAAGCAGTTTGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

268

Amino Acids

28.96

Weight (kDa)

8.05

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 78 - 163 6.5e-17 Lytic transglycolase
Expansin_C PF01357 174 - 251 8e-30 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 759
AccII CGCG 5 cut(s) 138, 168, 173, 185, 278
AcoI YGGCCR 2 cut(s) 262, 345
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 6 cut(s) 208, 371, 390, 436, 537, 759
AgsI TTSAA 3 cut(s) 618, 682, 782
AjnI CCWGG 1 cut(s) 201
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 404, 436
AluBI AGCT 1 cut(s) 467
AluI AGCT 1 cut(s) 467
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 582
AoxI GGCC 5 cut(s) 210, 262, 345, 394, 438
ApeKI GCWGC 2 cut(s) 294, 714
ArsI GACNNNNNNTTYG 2 cut(s) 637, 669
AspLEI GCGC 5 cut(s) 140, 185, 250, 278, 362
AspS9I GGNCC 3 cut(s) 289, 394, 424
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 3 cut(s) 91, 217, 370
AvaII GGWCC 2 cut(s) 289, 424
BalI TGGCCA 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 281, 726
BccI CCATC 3 cut(s) 341, 530, 713
BceAI ACGGC 3 cut(s) 39, 211, 360
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 564
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 582
BfaI CTAG 2 cut(s) 543, 711
BfmI CTRYAG 1 cut(s) 597
BfoI RGCGCY 1 cut(s) 251
BglII AGATCT 1 cut(s) 267
BisI GCNGC 5 cut(s) 153, 181, 274, 295, 715
BlpI GCTNAGC 1 cut(s) 239
BlsI GCNGC 5 cut(s) 154, 182, 275, 296, 716
Bme1390I CCNGG 2 cut(s) 203, 324
Bme18I GGWCC 2 cut(s) 289, 424
BmgT120I GGNCC 3 cut(s) 289, 394, 424
BmiI GGNNCC 2 cut(s) 328, 395
BmrFI CCNGG 2 cut(s) 203, 324
BpmI CTGGAG 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 384
BsaXI ACNNNNNCTCC 4 cut(s) 45, 75, 140, 170
Bsc4I CCNNNNNNNGG 6 cut(s) 208, 371, 390, 436, 537, 759
Bse118I RCCGGY 2 cut(s) 212, 578
Bse1I ACTGG 4 cut(s) 484, 589, 628, 665
Bse3DI GCAATG 1 cut(s) 577
Bse8I GATNNNNATC 1 cut(s) 455
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 384
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 6 cut(s) 208, 371, 390, 436, 537, 759
BseMI GCAATG 1 cut(s) 577
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 4 cut(s) 484, 589, 628, 665
BseRI GAGGAG 3 cut(s) 23, 53, 531
BseXI GCAGC 2 cut(s) 281, 726
Bsh1236I CGCG 5 cut(s) 138, 168, 173, 185, 278
BshFI GGCC 5 cut(s) 212, 264, 347, 396, 440
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 3 cut(s) 213, 324, 579
BslFI GGGAC 1 cut(s) 654
BslI CCNNNNNNNGG 6 cut(s) 208, 371, 390, 436, 537, 759
BsmAI GTCTC 1 cut(s) 582
BsmFI GGGAC 1 cut(s) 654
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 5 cut(s) 212, 264, 347, 396, 440
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 3 cut(s) 267, 450, 564
Bsp1720I GCTNAGC 1 cut(s) 239
Bsp68I TCGCGA 1 cut(s) 168
BspANI GGCC 5 cut(s) 212, 264, 347, 396, 440
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 5 cut(s) 138, 168, 173, 185, 278
BspLI GGNNCC 2 cut(s) 328, 395
BspMAI CTGCAG 1 cut(s) 601
BsrDI GCAATG 1 cut(s) 577
BsrFI RCCGGY 2 cut(s) 212, 578
BsrI ACTGG 4 cut(s) 484, 589, 628, 665
BssAI RCCGGY 2 cut(s) 212, 578
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 3 cut(s) 267, 450, 564
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 3 cut(s) 232, 401, 730
BstC8I GCNNGC 4 cut(s) 136, 187, 280, 580
BstDEI CTNAG 1 cut(s) 239
BstENI CCTNNNNNAGG 1 cut(s) 206
BstFNI CGCG 5 cut(s) 138, 168, 173, 185, 278
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 5 cut(s) 140, 185, 250, 278, 362
BstKTI GATC 3 cut(s) 270, 453, 567
BstMAI GTCTC 1 cut(s) 582
BstMBI GATC 3 cut(s) 267, 450, 564
BstMWI GCNNNNNNNGC 5 cut(s) 186, 195, 256, 270, 279
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 310
BstSCI CCNGG 2 cut(s) 201, 322
BstSFI CTRYAG 1 cut(s) 597
BstUI CGCG 5 cut(s) 138, 168, 173, 185, 278
BstV1I GCAGC 2 cut(s) 281, 726
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
BsuRI GGCC 5 cut(s) 212, 264, 347, 396, 440
BtgZI GCGATG 1 cut(s) 732
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 4 cut(s) 397, 564, 582, 606
BtuMI TCGCGA 1 cut(s) 168
Cac8I GCNNGC 4 cut(s) 136, 187, 280, 580
CfoI GCGC 5 cut(s) 140, 185, 250, 278, 362
Cfr10I RCCGGY 2 cut(s) 212, 578
Cfr13I GGNCC 3 cut(s) 289, 394, 424
CseI GACGC 3 cut(s) 25, 179, 286
CsiI ACCWGGT 1 cut(s) 201
Csp6I GTAC 1 cut(s) 193
CviAII CATG 1 cut(s) 307
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 239
DpnI GATC 3 cut(s) 269, 452, 566
DpnII GATC 3 cut(s) 267, 450, 564
EaeI YGGCCR 2 cut(s) 262, 345
Eco147I AGGCCT 1 cut(s) 440
Eco47I GGWCC 2 cut(s) 289, 424
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 201
FaeI CATG 1 cut(s) 310
FaiI YATR 7 cut(s) 308, 372, 476, 533, 593, 666, 668
FaqI GGGAC 1 cut(s) 654
FatI CATG 1 cut(s) 306
FauI CCCGC 1 cut(s) 182
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 5 cut(s) 153, 181, 274, 295, 715
Fsp4HI GCNGC 5 cut(s) 153, 181, 274, 295, 715
FspBI CTAG 2 cut(s) 543, 711
GlaI GCGC 5 cut(s) 139, 184, 249, 277, 361
GluI GCNGC 5 cut(s) 153, 181, 274, 295, 715
GsuI CTGGAG 1 cut(s) 606
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 5 cut(s) 212, 264, 347, 396, 440
HapII CCGG 3 cut(s) 213, 324, 579
HgaI GACGC 3 cut(s) 25, 179, 286
HhaI GCGC 5 cut(s) 140, 185, 250, 278, 362
Hin1II CATG 1 cut(s) 310
Hin6I GCGC 5 cut(s) 138, 183, 248, 276, 360
HinP1I GCGC 5 cut(s) 138, 183, 248, 276, 360
HinfI GANTC 3 cut(s) 104, 685, 797
HpaII CCGG 3 cut(s) 213, 324, 579
HphI GGTGA 3 cut(s) 91, 217, 370
Hpy188I TCNGA 3 cut(s) 160, 221, 411
Hpy188III TCNNGA 5 cut(s) 167, 430, 460, 618, 801
Hpy99I CGWCG 3 cut(s) 19, 173, 302
HpyAV CCTTC 6 cut(s) 47, 87, 106, 202, 506, 776
HpyCH4III ACNGT 3 cut(s) 232, 401, 730
HpyCH4V TGCA 2 cut(s) 318, 599
HpyF10VI GCNNNNNNNGC 5 cut(s) 186, 195, 256, 270, 279
HpyF3I CTNAG 1 cut(s) 239
Hsp92II CATG 1 cut(s) 310
HspAI GCGC 5 cut(s) 138, 183, 248, 276, 360
KroI GCCGGC 1 cut(s) 578
KroNI GCCGGC 1 cut(s) 580
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 3 cut(s) 267, 450, 564
LmnI GCTCC 3 cut(s) 36, 367, 754
Lsp1109I GCAGC 2 cut(s) 281, 726
MabI ACCWGGT 1 cut(s) 201
MaeI CTAG 2 cut(s) 543, 711
MaeIII GTNAC 3 cut(s) 401, 706, 730
MalI GATC 3 cut(s) 269, 452, 566
MboI GATC 3 cut(s) 267, 450, 564
MboII GAAGA 5 cut(s) 72, 75, 460, 616, 694
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 2 cut(s) 646, 757
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 264
MroNI GCCGGC 1 cut(s) 578
MscI TGGCCA 1 cut(s) 264
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 669
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 3 cut(s) 213, 324, 579
MspR9I CCNGG 2 cut(s) 203, 324
Mva1269I GAATGC 1 cut(s) 318
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 5 cut(s) 138, 168, 173, 185, 278
MwoI GCNNNNNNNGC 5 cut(s) 186, 195, 256, 270, 279
NaeI GCCGGC 1 cut(s) 580
NciI CCSGG 1 cut(s) 324
NdeII GATC 3 cut(s) 267, 450, 564
NgoMIV GCCGGC 1 cut(s) 578
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 2 cut(s) 328, 395
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 706
NruI TCGCGA 1 cut(s) 168
NspI RCATGY 1 cut(s) 310
PceI AGGCCT 1 cut(s) 440
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 156
PctI GAATGC 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 580
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 1 cut(s) 759
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 5 cut(s) 154, 182, 275, 296, 716
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 328, 395
PspPI GGNCC 3 cut(s) 289, 394, 424
PstI CTGCAG 1 cut(s) 601
PsuI RGATCY 1 cut(s) 267
RruI TCGCGA 1 cut(s) 168
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 669
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 5 cut(s) 153, 181, 274, 295, 715
Sau3AI GATC 3 cut(s) 267, 450, 564
Sau96I GGNCC 3 cut(s) 289, 394, 424
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 2 cut(s) 203, 324
SduI GDGCHC 1 cut(s) 33
SetI ASST 7 cut(s) 98, 146, 204, 291, 332, 469, 708
SexAI ACCWGGT 1 cut(s) 201
SfcI CTRYAG 1 cut(s) 597
SinI GGWCC 2 cut(s) 289, 424
SmiMI CAYNNNNRTG 1 cut(s) 669
Sse9I AATT 2 cut(s) 646, 757
SseBI AGGCCT 1 cut(s) 440
SspMI CTAG 2 cut(s) 543, 711
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 2 cut(s) 201, 322
TaaI ACNGT 3 cut(s) 232, 401, 730
TaqI TCGA 4 cut(s) 5, 255, 285, 300
TasI AATT 2 cut(s) 646, 757
TauI GCSGC 3 cut(s) 155, 183, 276
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 4 cut(s) 404, 564, 589, 606
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 2 cut(s) 294, 714
Tsp45I GTSAC 1 cut(s) 706
TspRI CASTG 4 cut(s) 404, 564, 589, 606
Van91I CCANNNNNTGG 1 cut(s) 759
VpaK11BI GGWCC 2 cut(s) 289, 424
XagI CCTNNNNNAGG 1 cut(s) 206
XceI RCATGY 1 cut(s) 310
XspI CTAG 2 cut(s) 543, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.