Rroxscaffold_6G00393860

expansin-A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010451
Physical Location & Seq
Forward (+)
14166404 .. 14171583
5180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393860.1

Sequence Viewer

Length: 783 bp
ATGTCGCCACCACCGAAGCTAACGGCGGTGCTCCTCCTCCTCCTATTCACACCACCCCTCCTCTCCCACTCGCCTTCTTCTTCTGCGATAACCTTCACCGACTCCCCCTCAATCTACGAATGGCAGTCCGCACGCGCCACCTACTACGCCGCCTCCGACCCTCGCGACGCTGTGGGCGGCGCGTGCGGCTACGGCGACCTGATGAAGGCCGGTTTCGGAATGGCGACGGTGGGGCTGAGCGAGGCGCTGTTCGAGCGTGGCCAGATCTGCGGCGCGTGCTTCGAGGTCCGCTGCGTCGAGGACATGCGGTGGTGCATTCCCGGAACCTCCATCATTTTGACGGCCACGAACTTCTGCGCTCCCAACTATGGCTTCACCGCCGAGGGTGGGGGCCACTCCCTGCGGCAGCTTAAACAATACTTTAGAAATTTAACCAATGCGAGTCTCAATACAATATGCAGACGACAAAAAAGGATAAAGTGTAGAAAGGAAGGAGGAGTTCGTTTTACCATAGATGGGTCTAGTATCTTTATTTCAGTGCTGATCAGCAATGTTGCCGGCACTGGAGACATAACTGCAGTGAAGATCAAGGGTTCAAGAACTGGTTGGCTTCCAATGGGACGAAATTGGGGTCAAAACTGGCATATAAATGCTGATTTGAAGAATCAGCCTCTTTCTTTTGAGGTCACTAGCAGCGATGGGAAGACAGTTACATCTTACAATGTCGCTCCCAAGAATTGGAACTTCGGGCAGACTTTTGAAGGGAAGCAGTTTGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

260

Amino Acids

28.35

Weight (kDa)

9.03

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 77 - 121 2e-07 Lytic transglycolase
Expansin_C PF01357 167 - 244 6.7e-30 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 738
AccII CGCG 4 cut(s) 135, 165, 182, 275
AcoI YGGCCR 2 cut(s) 259, 342
AcsI RAATTY 1 cut(s) 427
AfiI CCNNNNNNNGG 5 cut(s) 205, 368, 387, 516, 738
AgsI TTSAA 3 cut(s) 597, 661, 761
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
AluBI AGCT 2 cut(s) 19, 409
AluI AGCT 2 cut(s) 19, 409
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 449, 561
AoxI GGCC 4 cut(s) 207, 259, 342, 391
ApeKI GCWGC 3 cut(s) 291, 406, 693
ApoI RAATTY 1 cut(s) 427
ArsI GACNNNNNNTTYG 2 cut(s) 616, 648
AspLEI GCGC 5 cut(s) 137, 182, 247, 275, 359
AspS9I GGNCC 2 cut(s) 286, 391
AsuC2I CCSGG 1 cut(s) 321
AsuHPI GGTGA 2 cut(s) 88, 367
AvaII GGWCC 1 cut(s) 286
BalI TGGCCA 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 710
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 278, 418, 705
BccI CCATC 3 cut(s) 338, 509, 692
BceAI ACGGC 3 cut(s) 39, 208, 357
BclI TGATCA 1 cut(s) 543
BcnI CCSGG 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 449, 561
BfaI CTAG 2 cut(s) 522, 690
BfmI CTRYAG 1 cut(s) 576
BfoI RGCGCY 1 cut(s) 248
BglII AGATCT 1 cut(s) 264
BisI GCNGC 8 cut(s) 150, 178, 187, 271, 292, 404, 407, 694
BlpI GCTNAGC 1 cut(s) 236
BlsI GCNGC 8 cut(s) 151, 179, 188, 272, 293, 405, 408, 695
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 286, 391
BmiI GGNNCC 2 cut(s) 325, 392
BmrFI CCNGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 710
BpmI CTGGAG 1 cut(s) 585
Bpu1102I GCTNAGC 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 381
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 137, 167
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 368, 387, 516, 738
Bse118I RCCGGY 2 cut(s) 209, 557
Bse1I ACTGG 3 cut(s) 568, 607, 644
Bse3DI GCAATG 1 cut(s) 556
BseDI CCNNGG 1 cut(s) 381
BseLI CCNNNNNNNGG 5 cut(s) 205, 368, 387, 516, 738
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 3 cut(s) 568, 607, 644
BseRI GAGGAG 5 cut(s) 23, 26, 29, 50, 510
BseXI GCAGC 3 cut(s) 278, 418, 705
Bsh1236I CGCG 4 cut(s) 135, 165, 182, 275
BshFI GGCC 4 cut(s) 209, 261, 344, 393
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 3 cut(s) 210, 321, 558
BslFI GGGAC 1 cut(s) 633
BslI CCNNNNNNNGG 5 cut(s) 205, 368, 387, 516, 738
BsmAI GTCTC 2 cut(s) 449, 561
BsmFI GGGAC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 4 cut(s) 209, 261, 344, 393
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 3 cut(s) 264, 543, 585
Bsp1720I GCTNAGC 1 cut(s) 236
Bsp68I TCGCGA 1 cut(s) 165
BspANI GGCC 4 cut(s) 209, 261, 344, 393
BspCNI CTCAG 1 cut(s) 228
BspFNI CGCG 4 cut(s) 135, 165, 182, 275
BspLI GGNNCC 2 cut(s) 325, 392
BspMAI CTGCAG 1 cut(s) 580
BsrDI GCAATG 1 cut(s) 556
BsrFI RCCGGY 2 cut(s) 209, 557
BsrI ACTGG 3 cut(s) 568, 607, 644
BssAI RCCGGY 2 cut(s) 209, 557
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 3 cut(s) 264, 543, 585
Bst4CI ACNGT 2 cut(s) 229, 709
BstC8I GCNNGC 4 cut(s) 133, 184, 277, 559
BstDEI CTNAG 1 cut(s) 236
BstENI CCTNNNNNAGG 1 cut(s) 203
BstFNI CGCG 4 cut(s) 135, 165, 182, 275
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 5 cut(s) 137, 182, 247, 275, 359
BstKTI GATC 3 cut(s) 267, 546, 588
BstMAI GTCTC 2 cut(s) 449, 561
BstMBI GATC 3 cut(s) 264, 543, 585
BstMWI GCNNNNNNNGC 6 cut(s) 183, 186, 192, 253, 267, 276
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 576
BstUI CGCG 4 cut(s) 135, 165, 182, 275
BstV1I GCAGC 3 cut(s) 278, 418, 705
BstV2I GAAGAC 1 cut(s) 710
BstX2I RGATCY 1 cut(s) 264
BstYI RGATCY 1 cut(s) 264
BsuRI GGCC 4 cut(s) 209, 261, 344, 393
BtgZI GCGATG 1 cut(s) 711
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 3 cut(s) 543, 561, 585
BtuMI TCGCGA 1 cut(s) 165
Cac8I GCNNGC 4 cut(s) 133, 184, 277, 559
CfoI GCGC 5 cut(s) 137, 182, 247, 275, 359
Cfr10I RCCGGY 2 cut(s) 209, 557
Cfr13I GGNCC 2 cut(s) 286, 391
CseI GACGC 2 cut(s) 176, 283
CviAII CATG 1 cut(s) 304
DdeI CTNAG 1 cut(s) 236
DpnI GATC 3 cut(s) 266, 545, 587
DpnII GATC 3 cut(s) 264, 543, 585
EaeI YGGCCR 2 cut(s) 259, 342
Eco47I GGWCC 1 cut(s) 286
EcoNI CCTNNNNNAGG 1 cut(s) 203
FaeI CATG 1 cut(s) 307
FaiI YATR 7 cut(s) 305, 369, 457, 512, 572, 645, 647
FaqI GGGAC 1 cut(s) 633
FatI CATG 1 cut(s) 303
FbaI TGATCA 1 cut(s) 543
Fnu4HI GCNGC 8 cut(s) 150, 178, 187, 271, 292, 404, 407, 694
Fsp4HI GCNGC 8 cut(s) 150, 178, 187, 271, 292, 404, 407, 694
FspBI CTAG 2 cut(s) 522, 690
GlaI GCGC 5 cut(s) 136, 181, 246, 274, 358
GluI GCNGC 8 cut(s) 150, 178, 187, 271, 292, 404, 407, 694
GsuI CTGGAG 1 cut(s) 585
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 4 cut(s) 209, 261, 344, 393
HapII CCGG 3 cut(s) 210, 321, 558
HgaI GACGC 2 cut(s) 176, 283
HhaI GCGC 5 cut(s) 137, 182, 247, 275, 359
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 5 cut(s) 135, 180, 245, 273, 357
HinP1I GCGC 5 cut(s) 135, 180, 245, 273, 357
HinfI GANTC 4 cut(s) 101, 442, 664, 776
HpaII CCGG 3 cut(s) 210, 321, 558
HphI GGTGA 2 cut(s) 88, 367
Hpy188I TCNGA 2 cut(s) 157, 218
Hpy188III TCNNGA 3 cut(s) 164, 597, 780
Hpy99I CGWCG 3 cut(s) 170, 229, 299
HpyAV CCTTC 5 cut(s) 84, 103, 199, 485, 755
HpyCH4III ACNGT 2 cut(s) 229, 709
HpyCH4V TGCA 3 cut(s) 315, 459, 578
HpyF10VI GCNNNNNNNGC 6 cut(s) 183, 186, 192, 253, 267, 276
HpyF3I CTNAG 1 cut(s) 236
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 5 cut(s) 135, 180, 245, 273, 357
KroI GCCGGC 1 cut(s) 557
KroNI GCCGGC 1 cut(s) 559
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 3 cut(s) 264, 543, 585
LmnI GCTCC 3 cut(s) 36, 364, 733
LpnPI CCDG 9 cut(s) 212, 223, 275, 334, 413, 549, 571, 588, 625
Lsp1109I GCAGC 3 cut(s) 278, 418, 705
MaeI CTAG 2 cut(s) 522, 690
MaeIII GTNAC 2 cut(s) 685, 709
MalI GATC 3 cut(s) 266, 545, 587
MboI GATC 3 cut(s) 264, 543, 585
MboII GAAGA 5 cut(s) 69, 72, 595, 673, 715
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 3 cut(s) 427, 625, 736
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 2 cut(s) 95, 451
MmeI TCCRAC 1 cut(s) 180
Mox20I TGGCCA 1 cut(s) 261
MroNI GCCGGC 1 cut(s) 557
MscI TGGCCA 1 cut(s) 261
MseI TTAA 2 cut(s) 411, 431
MslI CAYNNNNRTG 1 cut(s) 648
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 291
MspI CCGG 3 cut(s) 210, 321, 558
MspR9I CCNGG 1 cut(s) 321
Mva1269I GAATGC 1 cut(s) 315
MvnI CGCG 4 cut(s) 135, 165, 182, 275
MwoI GCNNNNNNNGC 6 cut(s) 183, 186, 192, 253, 267, 276
NaeI GCCGGC 1 cut(s) 559
NciI CCSGG 1 cut(s) 321
NdeII GATC 3 cut(s) 264, 543, 585
NgoMIV GCCGGC 1 cut(s) 557
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 2 cut(s) 325, 392
NmeAIII GCCGAG 1 cut(s) 406
NmuCI GTSAC 1 cut(s) 685
NruI TCGCGA 1 cut(s) 165
NspI RCATGY 1 cut(s) 307
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 153
PctI GAATGC 1 cut(s) 315
PdiI GCCGGC 1 cut(s) 559
PfeI GAWTC 1 cut(s) 664
PflMI CCANNNNNTGG 1 cut(s) 738
PfoI TCCNGGA 1 cut(s) 319
PkrI GCNGC 8 cut(s) 151, 179, 188, 272, 293, 405, 408, 695
PleI GAGTC 2 cut(s) 95, 450
PpsI GAGTC 2 cut(s) 95, 450
PspN4I GGNNCC 2 cut(s) 325, 392
PspPI GGNCC 2 cut(s) 286, 391
PstI CTGCAG 1 cut(s) 580
PsuI RGATCY 1 cut(s) 264
RruI TCGCGA 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 648
SaqAI TTAA 2 cut(s) 411, 431
SatI GCNGC 8 cut(s) 150, 178, 187, 271, 292, 404, 407, 694
Sau3AI GATC 3 cut(s) 264, 543, 585
Sau96I GGNCC 2 cut(s) 286, 391
SchI GAGTC 2 cut(s) 95, 451
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 1 cut(s) 33
SetI ASST 8 cut(s) 21, 95, 143, 201, 288, 329, 411, 687
SfcI CTRYAG 1 cut(s) 576
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 648
Sse9I AATT 3 cut(s) 427, 625, 736
SspMI CTAG 2 cut(s) 522, 690
StyD4I CCNGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 229, 709
TaqI TCGA 3 cut(s) 252, 282, 297
TasI AATT 3 cut(s) 427, 625, 736
TauI GCSGC 5 cut(s) 152, 180, 189, 273, 406
TfiI GAWTC 1 cut(s) 664
Tru1I TTAA 2 cut(s) 411, 431
Tru9I TTAA 2 cut(s) 411, 431
TscAI CASTG 3 cut(s) 543, 568, 585
TseFI GTSAC 1 cut(s) 685
TseI GCWGC 3 cut(s) 291, 406, 693
Tsp45I GTSAC 1 cut(s) 685
TspDTI ATGAA 1 cut(s) 218
TspRI CASTG 3 cut(s) 543, 568, 585
Van91I CCANNNNNTGG 1 cut(s) 738
VpaK11BI GGWCC 1 cut(s) 286
XagI CCTNNNNNAGG 1 cut(s) 203
XapI RAATTY 1 cut(s) 427
XceI RCATGY 1 cut(s) 307
XspI CTAG 2 cut(s) 522, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.