MD17G1271500.v1.1

expansin-A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010451
Physical Location & Seq
Forward (+)
33231456 .. 33234226
2771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1271500.v1.1.491

Sequence Viewer

Length: 798 bp
ATGTGGCCGCCAGCTCTCCCCCTGGTCCTCCTCCTCCCACTCGCGCTCGCCTCCACCGCCCTCACCCACTCCCACTCCTCCTCCTTCTTCCCCACCGCCTCACCCTCACTCTACGACTGGAGCTCCGCGCGCGCCACCTACTACGCCGCCTCAGACCCCCGCGACAAGGTCGGCGGCGCGTGCGGCTACGGAGACCTCGAGAAGGCCGGGTACGGGCTGGCGACGGTGGGGCTGAGCGAGGCGCTGTTCGAGCGGGGCCAGATCTGCGGCGCATGCTTCCAGGTCCGGTGCGTGGAGGACACGCGGTGGTGCATTTCGGGGACGTCGATCATCGTGACGGCGACCAACTTCTGCGCGCCGAATTACGGGCTCAGGCCGGAGGGTGGGGGCCACTGCAACTCTCCGAACAAGCACTTGGTGTTGCCCATCGAGGCCTTTGAGAAGATCGCTGTTTGGAAGGCTGGCAATACGGCCGTCGAGTATCGCAGGATCAAGTGTAGAAAGGAAGGAGGAATTCGTTTCACCATAGATGGTTCTAACATCTTTATATCAGTACTCATCAGCAATGTTGCTGGTGCTGGAGACATAGTTGCGGTGAAGGTCAAGGGTTCAAGAACCGGTTGGCTTCCAATGGGACGGAATTGGGGCCAAAACTGGCATGTAAGTGCTGATTTACTGAACCAACCTCTTTCTTTCGAGGTCACAAGCAGCGATGGCCACACAGTTACATCTTACAATGTTGCTCCCAAGGCTTGGAACTTTGGGCAGACCTTTGAAGGAAAGCAGTTTGAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

266

Amino Acids

28.58

Weight (kDa)

8.1

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 76 - 161 8.6e-17 Lytic transglycolase
Expansin_C PF01357 172 - 249 1.9e-29 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 753
AccBSI CCGCTC 1 cut(s) 253
AccII CGCG 8 cut(s) 44, 128, 130, 132, 162, 179, 304, 356
AclWI GGATC 1 cut(s) 497
AcoI YGGCCR 3 cut(s) 5, 471, 715
AcsI RAATTY 1 cut(s) 513
AcyI GRCGYC 1 cut(s) 323
AdeI CACNNNGTG 2 cut(s) 306, 418
AfaI GTAC 2 cut(s) 212, 555
AfiI CCNNNNNNNGG 7 cut(s) 166, 202, 213, 292, 365, 383, 753
AgeI ACCGGT 1 cut(s) 617
AgsI TTSAA 2 cut(s) 612, 776
AjnI CCWGG 2 cut(s) 21, 279
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AluBI AGCT 2 cut(s) 14, 123
AluI AGCT 2 cut(s) 14, 123
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 2 cut(s) 186, 576
AlwI GGATC 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 9 cut(s) 5, 204, 256, 374, 388, 432, 471, 646, 715
ApeKI GCWGC 1 cut(s) 708
ApoI RAATTY 1 cut(s) 513
AsiGI ACCGGT 1 cut(s) 617
AspLEI GCGC 9 cut(s) 46, 130, 132, 134, 179, 244, 272, 356, 358
AspS9I GGNCC 5 cut(s) 25, 256, 283, 388, 646
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 4 cut(s) 55, 93, 514, 607
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 2 cut(s) 25, 283
BalI TGGCCA 1 cut(s) 717
BanII GRGCYC 2 cut(s) 125, 372
BarI GAAGNNNNNNTAC 2 cut(s) 194, 226
Bbv12I GWGCWC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 720
BccI CCATC 3 cut(s) 434, 524, 707
BceAI ACGGC 3 cut(s) 354, 458, 486
BciT130I CCWGG 2 cut(s) 23, 281
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 186, 576
BfoI RGCGCY 1 cut(s) 245
BglII AGATCT 1 cut(s) 261
BisI GCNGC 6 cut(s) 8, 147, 175, 184, 268, 709
BlpI GCTNAGC 1 cut(s) 233
BlsI GCNGC 6 cut(s) 9, 148, 176, 185, 269, 710
BmcAI AGTACT 1 cut(s) 555
Bme1390I CCNGG 3 cut(s) 23, 208, 281
Bme18I GGWCC 2 cut(s) 25, 283
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 5 cut(s) 25, 256, 283, 388, 646
BmiI GGNNCC 3 cut(s) 257, 389, 647
BmrFI CCNGG 3 cut(s) 23, 208, 281
BpmI CTGGAG 2 cut(s) 139, 600
Bpu10I CCTNAGC 1 cut(s) 371
Bpu1102I GCTNAGC 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 21, 747
BsaWI WCCGGW 2 cut(s) 285, 617
BsaXI ACNNNNNCTCC 8 cut(s) 59, 65, 89, 95, 107, 137, 573, 603
Bsc4I CCNNNNNNNGG 7 cut(s) 166, 202, 213, 292, 365, 383, 753
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 2 cut(s) 122, 659
Bse3DI GCAATG 1 cut(s) 571
BseBI CCWGG 2 cut(s) 23, 281
BseDI CCNNGG 2 cut(s) 21, 747
BseLI CCNNNNNNNGG 7 cut(s) 166, 202, 213, 292, 365, 383, 753
BseMI GCAATG 1 cut(s) 571
BseMII CTCAG 3 cut(s) 165, 224, 385
BseNI ACTGG 2 cut(s) 122, 659
BsePI GCGCGC 3 cut(s) 128, 130, 354
BseRI GAGGAG 4 cut(s) 20, 23, 67, 70
BseX3I CGGCCG 1 cut(s) 471
BseXI GCAGC 1 cut(s) 720
Bsh1236I CGCG 8 cut(s) 44, 128, 130, 132, 162, 179, 304, 356
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 9 cut(s) 7, 206, 258, 376, 390, 434, 473, 648, 717
BshTI ACCGGT 1 cut(s) 617
BsiEI CGRYCG 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 4 cut(s) 207, 286, 377, 618
BslFI GGGAC 2 cut(s) 334, 648
BslI CCNNNNNNNGG 7 cut(s) 166, 202, 213, 292, 365, 383, 753
BsmAI GTCTC 2 cut(s) 186, 576
BsmFI GGGAC 2 cut(s) 334, 648
BsnI GGCC 9 cut(s) 7, 206, 258, 376, 390, 434, 473, 648, 717
Bso31I GGTCTC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 2 cut(s) 125, 372
Bsp143I GATC 4 cut(s) 261, 327, 444, 489
Bsp1720I GCTNAGC 1 cut(s) 233
BspANI GGCC 9 cut(s) 7, 206, 258, 376, 390, 434, 473, 648, 717
BspCNI CTCAG 3 cut(s) 164, 225, 384
BspFNI CGCG 8 cut(s) 44, 128, 130, 132, 162, 179, 304, 356
BspLI GGNNCC 3 cut(s) 257, 389, 647
BspPI GGATC 1 cut(s) 497
BspTNI GGTCTC 1 cut(s) 186
BsrBI CCGCTC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 571
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 2 cut(s) 122, 659
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 2 cut(s) 21, 747
BssHII GCGCGC 3 cut(s) 128, 130, 354
BssMI GATC 4 cut(s) 261, 327, 444, 489
BssNI GRCGYC 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 747
Bst2UI CCWGG 2 cut(s) 23, 281
Bst4CI ACNGT 2 cut(s) 226, 724
BstACI GRCGYC 1 cut(s) 323
BstC8I GCNNGC 9 cut(s) 12, 48, 130, 132, 181, 219, 274, 356, 463
BstDEI CTNAG 3 cut(s) 151, 233, 371
BstENI CCTNNNNNAGG 1 cut(s) 200
BstFNI CGCG 8 cut(s) 44, 128, 130, 132, 162, 179, 304, 356
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 9 cut(s) 46, 130, 132, 134, 179, 244, 272, 356, 358
BstKTI GATC 4 cut(s) 264, 330, 447, 492
BstMAI GTCTC 2 cut(s) 186, 576
BstMBI GATC 4 cut(s) 261, 327, 444, 489
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 9 cut(s) 56, 129, 180, 183, 250, 264, 273, 714, 749
BstNI CCWGG 2 cut(s) 23, 281
BstNSI RCATGY 2 cut(s) 276, 662
BstSCI CCNGG 3 cut(s) 21, 206, 279
BstUI CGCG 8 cut(s) 44, 128, 130, 132, 162, 179, 304, 356
BstV1I GCAGC 1 cut(s) 720
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BstZI CGGCCG 1 cut(s) 471
BsuRI GGCC 9 cut(s) 7, 206, 258, 376, 390, 434, 473, 648, 717
BtgZI GCGATG 1 cut(s) 726
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 9 cut(s) 12, 48, 130, 132, 181, 219, 274, 356, 463
CfoI GCGC 9 cut(s) 46, 130, 132, 134, 179, 244, 272, 356, 358
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 5 cut(s) 25, 256, 283, 388, 646
Csp6I GTAC 2 cut(s) 211, 554
CspAI ACCGGT 1 cut(s) 617
CviAII CATG 2 cut(s) 273, 659
CviQI GTAC 2 cut(s) 211, 554
DdeI CTNAG 3 cut(s) 151, 233, 371
DpnI GATC 4 cut(s) 263, 329, 446, 491
DpnII GATC 4 cut(s) 261, 327, 444, 489
DraIII CACNNNGTG 2 cut(s) 306, 418
EaeI YGGCCR 3 cut(s) 5, 471, 715
EagI CGGCCG 1 cut(s) 471
Ecl136II GAGCTC 1 cut(s) 123
EclXI CGGCCG 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 747
Eco147I AGGCCT 1 cut(s) 434
Eco24I GRGCYC 2 cut(s) 125, 372
Eco31I GGTCTC 1 cut(s) 186
Eco47I GGWCC 2 cut(s) 25, 283
Eco52I CGGCCG 1 cut(s) 471
Eco53kI GAGCTC 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 197
EcoICRI GAGCTC 1 cut(s) 123
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 21, 279
EcoT14I CCWWGG 1 cut(s) 747
EcoT38I GRGCYC 2 cut(s) 125, 372
ErhI CCWWGG 1 cut(s) 747
FaeI CATG 2 cut(s) 276, 662
FaiI YATR 5 cut(s) 274, 527, 548, 587, 660
FaqI GGGAC 2 cut(s) 334, 648
FatI CATG 2 cut(s) 272, 658
FauI CCCGC 2 cut(s) 167, 246
Fnu4HI GCNGC 6 cut(s) 8, 147, 175, 184, 268, 709
FriOI GRGCYC 2 cut(s) 125, 372
Fsp4HI GCNGC 6 cut(s) 8, 147, 175, 184, 268, 709
GlaI GCGC 9 cut(s) 45, 129, 131, 133, 178, 243, 271, 355, 357
GluI GCNGC 6 cut(s) 8, 147, 175, 184, 268, 709
GsuI CTGGAG 2 cut(s) 139, 600
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 9 cut(s) 7, 206, 258, 376, 390, 434, 473, 648, 717
HapII CCGG 4 cut(s) 207, 286, 377, 618
HhaI GCGC 9 cut(s) 46, 130, 132, 134, 179, 244, 272, 356, 358
Hin1I GRCGYC 1 cut(s) 323
Hin1II CATG 2 cut(s) 276, 662
Hin6I GCGC 9 cut(s) 44, 128, 130, 132, 177, 242, 270, 354, 356
HinP1I GCGC 9 cut(s) 44, 128, 130, 132, 177, 242, 270, 354, 356
HinfI GANTC 1 cut(s) 791
HpaII CCGG 4 cut(s) 207, 286, 377, 618
HphI GGTGA 4 cut(s) 55, 93, 514, 607
Hpy188I TCNGA 2 cut(s) 154, 405
Hpy188III TCNNGA 3 cut(s) 199, 334, 612
Hpy99I CGWCG 3 cut(s) 226, 328, 479
HpyAV CCTTC 6 cut(s) 94, 196, 451, 500, 592, 770
HpyCH4III ACNGT 2 cut(s) 226, 724
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 2 cut(s) 312, 396
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 129, 180, 183, 250, 264, 273, 714, 749
HpyF3I CTNAG 3 cut(s) 151, 233, 371
HpySE526I ACGT 1 cut(s) 323
Hsp92I GRCGYC 1 cut(s) 323
Hsp92II CATG 2 cut(s) 276, 662
HspAI GCGC 9 cut(s) 44, 128, 130, 132, 177, 242, 270, 354, 356
Kzo9I GATC 4 cut(s) 261, 327, 444, 489
LmnI GCTCC 3 cut(s) 120, 128, 748
Lsp1109I GCAGC 1 cut(s) 720
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 3 cut(s) 334, 700, 724
MalI GATC 4 cut(s) 263, 329, 446, 491
MauBI CGCGCGCG 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 253
MboI GATC 4 cut(s) 261, 327, 444, 489
MboII GAAGA 2 cut(s) 79, 454
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 2 cut(s) 125, 372
MlsI TGGCCA 1 cut(s) 717
MluCI AATT 3 cut(s) 361, 513, 640
MluNI TGGCCA 1 cut(s) 717
Mox20I TGGCCA 1 cut(s) 717
MscI TGGCCA 1 cut(s) 717
MseI TTAA 1 cut(s) 796
MslI CAYNNNNRTG 1 cut(s) 663
Msp20I TGGCCA 1 cut(s) 717
MspI CCGG 4 cut(s) 207, 286, 377, 618
MspR9I CCNGG 3 cut(s) 23, 208, 281
MvaI CCWGG 2 cut(s) 23, 281
MvnI CGCG 8 cut(s) 44, 128, 130, 132, 162, 179, 304, 356
MwoI GCNNNNNNNGC 9 cut(s) 56, 129, 180, 183, 250, 264, 273, 714, 749
NciI CCSGG 1 cut(s) 208
NdeII GATC 4 cut(s) 261, 327, 444, 489
NlaIII CATG 2 cut(s) 276, 662
NlaIV GGNNCC 3 cut(s) 257, 389, 647
NmuCI GTSAC 2 cut(s) 334, 700
NspI RCATGY 2 cut(s) 276, 662
PaeI GCATGC 1 cut(s) 276
PaeR7I CTCGAG 1 cut(s) 197
PauI GCGCGC 3 cut(s) 128, 130, 354
PceI AGGCCT 1 cut(s) 434
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 323
PflFI GACNNNGTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 753
PinAI ACCGGT 1 cut(s) 617
PkrI GCNGC 6 cut(s) 9, 148, 176, 185, 269, 710
Psp124BI GAGCTC 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 21, 279
PspGI CCWGG 2 cut(s) 21, 279
PspN4I GGNNCC 3 cut(s) 257, 389, 647
PspPI GGNCC 5 cut(s) 25, 256, 283, 388, 646
PsuI RGATCY 1 cut(s) 261
PsyI GACNNNGTC 1 cut(s) 167
PteI GCGCGC 3 cut(s) 128, 130, 354
RsaI GTAC 2 cut(s) 212, 555
RsaNI GTAC 2 cut(s) 211, 554
RseI CAYNNNNRTG 1 cut(s) 663
SacI GAGCTC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 796
SatI GCNGC 6 cut(s) 8, 147, 175, 184, 268, 709
Sau3AI GATC 4 cut(s) 261, 327, 444, 489
Sau96I GGNCC 5 cut(s) 25, 256, 283, 388, 646
ScaI AGTACT 1 cut(s) 555
ScrFI CCNGG 3 cut(s) 23, 208, 281
SduI GDGCHC 2 cut(s) 125, 372
Sfr274I CTCGAG 1 cut(s) 197
SinI GGWCC 2 cut(s) 25, 283
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SphI GCATGC 1 cut(s) 276
Sse9I AATT 3 cut(s) 361, 513, 640
SseBI AGGCCT 1 cut(s) 434
SstI GAGCTC 1 cut(s) 125
StuI AGGCCT 1 cut(s) 434
StyD4I CCNGG 3 cut(s) 21, 206, 279
StyI CCWWGG 1 cut(s) 747
TaaI ACNGT 2 cut(s) 226, 724
TaiI ACGT 1 cut(s) 326
TaqI TCGA 6 cut(s) 198, 249, 326, 429, 477, 696
TasI AATT 3 cut(s) 361, 513, 640
TatI WGTACW 1 cut(s) 553
TauI GCSGC 5 cut(s) 10, 149, 177, 186, 270
Tru1I TTAA 1 cut(s) 796
Tru9I TTAA 1 cut(s) 796
TscAI CASTG 1 cut(s) 398
TseFI GTSAC 2 cut(s) 334, 700
TseI GCWGC 1 cut(s) 708
Tsp45I GTSAC 2 cut(s) 334, 700
TspGWI ACGGA 2 cut(s) 204, 652
TspRI CASTG 1 cut(s) 398
Tth111I GACNNNGTC 1 cut(s) 167
Van91I CCANNNNNTGG 1 cut(s) 753
VpaK11BI GGWCC 2 cut(s) 25, 283
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 1 cut(s) 513
XceI RCATGY 2 cut(s) 276, 662
XhoI CTCGAG 1 cut(s) 197
ZraI GACGTC 1 cut(s) 324
ZrmI AGTACT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.